eLife

Latest preprint reviews

1. Single-cell and spatial transcriptomic analyses reveals the dynamic transcript profiles of myocardial lymphangiogenesis post-myocardial infarction

   This article has 12 authors:

   1. Jiaqi He
   2. Dali Zhang
   3. Haixu Song
   4. Ziqi Liu
   5. Dan Liu
   6. Xiaolin Zhang
   7. Xiaojie Zhao
   8. Yan Zhang
   9. Jing Liu
   10. Jiaxin Xu
   11. Chenghui Yan
   12. Yaling Han

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study presents useful albeit preliminary findings on transcriptome changes in cardiac lymphatic cells after myocardial infarction in mice. Despite revision, the conclusions of the authors remain uncertain as sample sizes in general are very low, and even sometimes too low to allow for valid statistical comparisons. Accordingly, there are concerns regarding statistical robustness, raised by both the editors and the reviewers. While the single-cell transcriptomic data were analyzed using solid advanced methodology, too few cells were included in the scRNA-seq data set and the spatial transcriptomics analyses. Thus, this study rather represents more a collection of preliminary transcriptomic data than a full scientific report that would definitively advance the field.

   Reviewed by eLife

2. Natural variation in salt-induced changes in root:shoot ratio reveals SR3G as a negative regulator of root suberization and salt resilience in Arabidopsis

   This article has 18 authors:

   1. Maryam Rahmati Ishka
   2. Hayley Sussman
   3. Yunfei Hu
   4. Mashael Daghash Alqahtani
   5. Eric Craft
   6. Ronell Sicat
   7. Minmin Wang
   8. Li'ang Yu
   9. Rachid Ait-Haddou
   10. Bo Li
   11. Georgia Drakakaki
   12. Andrew DL Nelson
   13. Miguel Pineros
   14. Arthur Korte
   15. Łukasz Jaremko
   16. Christa Testerink
   17. Mark Tester
   18. Magdalena M Julkowska

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     Through cellular, developmental, and physiological analysis, this valuable study identifies a gene that regulates the relative growth of roots and shoots under salt stress. The holistic approach taken provides convincing evidence that this member of a larger tandemly duplicated gene family together with an upstream regulator contributes to salt tolerance. The manuscript will be of interest to plant biologists studying mechanisms of abiotic stress tolerance and gene family evolution.

   Reviewed by eLife

3. Real-time transcriptomic profiling in distinct experimental conditions

   This article has 12 authors:

   1. Tamer Butto
   2. Stefan Pastore
   3. Max Müller
   4. Kaushik Viswanathan Iyer
   5. Marko Jörg
   6. Julia Brechtel
   7. Stefan Mündnich
   8. Anna Wierczeiko
   9. Kristina Friedland
   10. Mark Helm
   11. Marie-Luise Winz
   12. Susanne Gerber

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This useful study presents a real-time transcriptomics analysis, with the aim of providing rapid access to sequenced data to reduce the costs associated with Oxford Nanopore long-read technology. The revised manuscript demonstrates the utilities with four sets of experiments with convincing evidence.

   Reviewed by eLife

4. Direct lineage conversion of postnatal mouse cortical astrocytes to oligodendrocyte lineage cells

   This article has 12 authors:

   1. Justine Bajohr
   2. Erica Y Scott
   3. Arman Olfat
   4. Mehrshad Sadria
   5. Kevin Lee
   6. Maria Fahim
   7. Hiba T Taha
   8. Daniela Lozano Casasbuenas
   9. Ann Derham
   10. Scott A Yuzwa
   11. Gary D Bader
   12. Maryam Faiz

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study provides a useful advance in generating mouse oligodendrocytes by direct lineage conversion from cortical astrocytes. The authors demonstrate that Sox10 converts astrocytes to MBP+ oligodendrocytes, whereas Olig2 expression converts astrocytes to PDFRalpha+ oligodendrocyte progenitor cells. The data supporting the conclusions are solid, but there are concerns regarding select figures and the absence of functional validation.

   Reviewed by eLife

5. Distinct release properties of glutamate/GABA co-transmission serve as a frequency-dependent filtering of supramammillary inputs

   This article has 6 authors:

   1. Himawari Hirai
   2. Kohtarou Konno
   3. Miwako Yamasaki
   4. Masahiko Watanabe
   5. Takeshi Sakaba
   6. Yuki Hashimotodani

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This fundamental work provides evidence that glutamate and GABA are released from different synaptic vesicles at supramammillary axon terminals onto granule cells of the dentate gyrus. The study uses complementary electrophysiological and anatomical experimental approaches. Together, these provide convincing evidence that the co-release of glutamate and GABA from different vesicles within the same terminal could modulate granule cell firing in a frequency-dependent manner, although thorough elimination of alternative mechanisms would have strengthened the study. The work will be of interest to neuroscientists investigating co-release of neurotransmitters in various synapses in the brain and those interested in subcortical control of hippocampal function.

   Reviewed by eLife

6. Remote automated delivery of mechanical stimuli coupled to brain recordings in behaving mice

   This article has 23 authors:

   1. Justin Burdge
   2. Anissa Jhumka
   3. Ashar Khan
   4. Simon Ogundare
   5. Nicholas Baer
   6. Sasha Fulton
   7. Alexander Kaplan
   8. Brittany Bistis
   9. William Foster
   10. Joshua Thackray
   11. Andre Toussaint
   12. Miao Li
   13. Yosuke M Morizawa
   14. Jake Nazarian
   15. Leah Yadessa
   16. Arlene J George
   17. Abednego Delinois
   18. Wadzanayi Mayiseni
   19. Noah Loran
   20. Guang Yang
   21. David J Margolis
   22. Victoria E Abraira
   23. Ishmail Abdus-Saboor

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This important study describes the development and validation of an Automated Reproducible Mechano-stimulator (ARM), a tool for standardizing and automating tactile behavior experiments. The data supporting the use of the ARM system are compelling, and demonstrate that by removing experimenter effects on animals, it reduces variability in various parameters of stimulus application. Moreover, the authors demonstrate that any noise emitted from the ARM does not induce an increased stress state. Once commercially available, the ARM system has the potential to increase experimental reproducibility between laboratories in the somatosentation and pain fields.

   Reviewed by eLife

7. Mapping serotonergic dynamics using drug-modulated molecular connectivity in rats

   This article has 7 authors:

   1. Tudor M Ionescu
   2. Mario Amend
   3. Rakibul Hafiz
   4. Andreas Maurer
   5. Bharat Biswal
   6. Hans F Wehrl
   7. Kristina Herfert

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This important paper on measuring molecular connectivity using combined serotonin PET and resting-state fMRI provides both novel methods for studying the brain as well as insights into the effects of ecstasy administration. The methods are convincing, with the high anaesthetic dose used likely limiting network activity.

   Reviewed by eLife

8. Salmonids elicit an acute behavioral response to heterothermal environments

   This article has 7 authors:

   1. Robert Naudascher
   2. Stefano Brizzolara
   3. Jonasz Slomka
   4. Robert M Boes
   5. Markus Holzner
   6. Luiz GM Silva
   7. Roman Stocker

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This valuable paper investigates how fish avoid thermal disturbances that occur on fast timescales. The authors use a creative experimental approach that quickly creates a vertical thermal interface, which they combine with careful behavioral analyses. The evidence supporting their results is solid, but there is a potential confounding factor between temperature and vertical positioning, and characterization of the thermal interface would greatly assist in interpreting the results.

   Reviewed by eLife

9. Fish CDK2 recruits Dtx4 to degrade TBK1 through ubiquitination in the antiviral response

   This article has 16 authors:

   1. Long-Feng Lu
   2. Can Zhang
   3. Zhuo-Cong Li
   4. Bao-Jie Cui
   5. Yang-Yang Wang
   6. Ke-Jia Han
   7. Xiao Xu
   8. Chu-Jing Zhou
   9. Xiao-Yu Zhou
   10. Yue Wu
   11. Na Xu
   12. Xiao-Li Yang
   13. Dan-Dan Chen
   14. Xiyin Li
   15. Li Zhou
   16. Shun Li

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This valuable study uses zebrafish as a model to reveal a role for the cell cycle protein kinase CDK2 as a negative regulator of type I interferon signaling. The evidence supporting the authors' claims is convincing, including both in vivo and in vitro investigative approaches that corroborate a role for CDK2 in regulating TBK1 degradation. In this latest version, the authors included data addressing a concern raised by the reviewer in the previous peer review round. This work will interest cell biologists, immunologists, and virologists.

   Reviewed by eLife

10. Defining mononuclear phagocyte distribution and behaviour in the zebrafish heart

    This article has 6 authors:

    1. Bethany Moyse
    2. Joanna Moss
    3. Laura Bevan
    4. Aaron Scott
    5. Valérie Wittamer
    6. Rebecca J Richardson

    This article has been curated by 1 group:

    • Curated by eLife

      eLife assessment

      The manuscript presented by Moyse and colleagues provides valuable insight into the origin, morphology, dynamics, and behavior of several populations of mononuclear phagocytes in the zebrafish heart. The study presents solid evidence through the use of transgenic lines and live imaging, although some limitations related to lineage tracing and molecular profiles should be considered. This work exemplifies the use of zebrafish as a model to study the role of leukocytes in cardiac development and regeneration and potentially draw broader interest to biologists working in immunology fields.

    Reviewed by eLife