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  1. A Sterol-Binding Cavity Underlies Sterol Recognition and Differential Activation of the ABCG5/G8

    1. Fatemeh Rezaei
    2. Isra F. Omar
    3. Danny Farhat
    4. Zi-Wen Weng
    5. Kadambari Vijay Sai
    6. Qing-Fang Xiao
    7. Yuan-Chih Chang
    8. Shang-Te Danny Hsu
    9. Jyh-Yeuan Lee

    • Curated by eLife

      eLife Assessment

      This work presents valuable findings that identify ergosterol binding by the human ABC transporter ABCG5/G8 and show that ergosterol stimulates its ATPase activity more strongly than cholesterol, advancing understanding of sterol recognition and transport. The evidence from single-particle cryo-EM, molecular dynamics simulations, and biochemical assays is convincing and solid, although additional structures and mutational studies would strengthen the proposed mechanism. These results provide key insight into the molecular basis of sterol recognition and regulation of ABCG5/G8 and will be of interest to researchers studying sterol transport and ABC transporter function.

    Reviewed by eLife

    5 evaluationsAppears in 1 listLatest version Latest activity

  5. Phylogenomics of free-living neobodonids reveals they are a paraphyletic group from which all other metakinetoplastids are descended

    1. Daryna Zavadska
    2. Julia A. Packer
    3. Daria Tashyreva
    4. Ryoma Kamikawa
    5. Alastair Simpson
    6. Daniel J. Richter

    • Curated by eLife

      eLife Assessment

      This valuable study substantially improves the phylogenetic framework for Kinetoplastea through greatly expanded sampling of free-living neobodonids; the phylogenomic analyses provide convincing evidence that neobodonids are paraphyletic and establish a substantially revised framework for deep relationships within Metakinetoplastina. The work forms the foundation for future comparative studies of kinetoplastid evolution and systematics, although its implications for the origins of parasitism should not be overstated. An 18S rRNA phylogeny documenting the placement of the newly sampled strains within previously recognized neobodonid lineages and/or confirmation of the principal topology using a well-documented phylogenomic set of marker genes would further strengthen the study. The work will be of particular interest to researchers studying protist evolution and the evolutionary context of trypanosomatids.

    Reviewed by eLife

    5 evaluationsAppears in 1 listLatest version Latest activity
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