eLife reviews preprints in the life sciences and medicine.

About Followers

Latest preprint reviews

  2. Phylogenomics of free-living neobodonids reveals they are a paraphyletic group from which all other metakinetoplastids are descended

    1. Daryna Zavadska
    2. Julia A. Packer
    3. Daria Tashyreva
    4. Ryoma Kamikawa
    5. Alastair Simpson
    6. Daniel J. Richter

    • Curated by eLife

      eLife Assessment

      This valuable study substantially improves the phylogenetic framework for Kinetoplastea through greatly expanded sampling of free-living neobodonids; the phylogenomic analyses provide convincing evidence that neobodonids are paraphyletic and establish a substantially revised framework for deep relationships within Metakinetoplastina. The work forms the foundation for future comparative studies of kinetoplastid evolution and systematics, although its implications for the origins of parasitism should not be overstated. An 18S rRNA phylogeny documenting the placement of the newly sampled strains within previously recognized neobodonid lineages and/or confirmation of the principal topology using a well-documented phylogenomic set of marker genes would further strengthen the study. The work will be of particular interest to researchers studying protist evolution and the evolutionary context of trypanosomatids.

    Reviewed by eLife

    5 evaluationsAppears in 1 listLatest version Latest activity

  3. Phosphorylation and pocket-binding ligands rewire the coupled networks in multidomain protein condensate

    1. Yu Cao
    2. Jinyu Chen
    3. Xiakun Chu

    • Curated by eLife

      eLife Assessment

      This important study uses coarse-grained molecular simulations to investigate how phosphorylation and small-molecule ligands reshape the interactions underlying condensation and multidomain assembly of HDAC6. The findings provide mechanistic insights into how modifications of intrinsically disordered regions and ligand binding may regulate protein condensation. The evidence is solid: although the simulations broadly agree with previous experimental observations, concerns about model robustness and sampling in full-length assemblies limit support for the proposed mechanism and its pharmacological implications.

    Reviewed by eLife

    5 evaluationsAppears in 1 listLatest version Latest activity

  4. Penumbria: Advanced 3D cell segmentation for biomedical imaging

    1. Laurids Stockert
    2. Joseph Donovan
    3. Herwig Baier

    • Curated by eLife

      eLife Assessment

      This useful study presents Penumbria, a method for segmenting cells in three-dimensional microscopy images, released with publicly available code and documentation. The implementation is technically sound, and the reported performance gains would be a welcome contribution; the advance is incremental, since regression on distances to boundaries is already widespread in the field. The evidence is incomplete: the main text does not state which setting it used for the main competing method, and the setting used is the one that handles data of this kind poorly, so the central claim of better accuracy is unsettled; current leading methods are missing from the comparison; and five architectural and training choices are combined without attributing the gains to any of them. Every evaluation dataset labels nuclei, so the work should present itself as nuclei segmentation; describing and completing the comparisons, and attributing the gains, would raise the strength of evidence.

    Reviewed by eLife

    4 evaluationsAppears in 1 listLatest version Latest activity

  5. Wnt/beta-catenin signaling regulates Vascular Endothelial Growth Factor (VEGF) receptors in central nervous system endothelial cells

    1. Yanshu Wang
    2. Ningyu Zhu
    3. Amir Rattner
    4. Philip M. Smallwood
    5. Jeremy Nathans

    • Curated by eLife

      eLife Assessment

      This study provides important novel insight into how Wnt/beta-catenin signaling may regulate the responsiveness of blood vessels in the brain and retina to vascular endothelial growth factor, with potential relevance to Wnt surrogate therapies aimed at restoring vascular barriers. Solid evidence that Wnt/beta-catenin signaling changes the protein abundance and relative balance of two vascular endothelial growth factor receptors in brain blood vessels is provided by informative genetic mosaic experiments; in the retina, however, regulation of VEGFR2 was difficult to establish because expression by neighboring cells obscured its detection in blood vessels. Physiological implications are discussed, but evidence remains incomplete because the observed changes in receptor abundance were not directly linked to altered signaling or vascular function in living animals. A second mechanism, in which Wnt/beta-catenin signaling suppresses signaling through VEGFR2 with little or no change in receptor abundance, was inferred from reporter experiments in cell-based systems.

    Reviewed by eLife

    4 evaluationsAppears in 1 listLatest version Latest activity

  8. Whole human organ clearing and multimodal mapping

    1. Héloïse Policet-Bétend
    2. Tomàs Jordà-Siquier
    3. Geraldine Cuenu
    4. Maeva Badré
    5. Sami Schranz
    6. Julien Da Costa
    7. Céline Brockmann
    8. Pascal Senn
    9. Daniel Huber
    10. Christophe M Lamy

    • Curated by eLife

      eLife Assessment

      This important paper introduces a generalisable tissue-clearing method to study human anatomy and the organisation of complex tissues and organs. The evidence for its broad applicability is compelling, based on results from 15 diverse human tissues, including highly heterogeneous specimens, with successful labelling using five antibodies and five fluorescent dyes. The method has broad potential applications across multiple disciplines.

    Reviewed by eLife

    3 evaluationsAppears in 1 listLatest version Latest activity
Page 1 of 870 Older