eLife

Latest preprint reviews

1. Use-dependent regulation of the axonal action potential in parvalbumin-expressing interneurons

   This article has 2 authors:

   1. Sophie R Liebergall
   2. Ethan M Goldberg

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This important study shows that action potentials undergo frequency-dependent failure along the axons of fast-spiking interneurons during sustained high-frequency firing, offering a mechanistic explanation for why inhibition may fail to restrain seizures. The evidence is solid, though additional analyses could further strengthen the mechanistic interpretation. The work will be of broad interest to neuroscientists studying axonal physiology, cortical inhibition, and epilepsy.

   Reviewed by eLife

2. Functional characterization of a multi-cancer risk locus on chromosome band 2q33.1 near CASP8

   This article has 20 authors:

   1. Hyunkyung Kong
   2. Jiyeon Choi
   3. Tongwu Zhang
   4. Cathrin Gräwe
   5. Mai Xu
   6. Rohit Thakur
   7. Hayley Sowards
   8. Rebecca C Hennessey
   9. Andrew Vu
   10. Jianxin Shi
   11. D Timothy Bishop
   12. Julia Newton-Bishop
   13. Jeremie Nsengimana
   14. Mark M Iles
   15. Maria Teresa Landi
   16. Michiel Vermeulen
   17. Matthew H Law
   18. Laufey T Amundadottir
   19. Melanoma Meta-Analysis Consortium
   20. Kevin M Brown

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This important study by Kong et al. systematically and rigorously dissects the gene regulatory network underlying melanoma and breast cancer risk at the multi-cancer 2q33 locus. The authors provide compelling evidence that rs3769823 is a key functional variant that acts through allele-preferential binding of the transcription factors E4F1 and IRF2 to regulate CASP8 and FLACC1 in a cell-type-specific manner. The work makes a significant contribution to understanding the mechanisms operating at multi-cancer risk loci.

   Reviewed by eLife

3. Metabolic basis of the astrocyte-synapse interaction governs dopaminergic-motor connection

   This article has 5 authors:

   1. Yanru Xu
   2. Piaoping Kong
   3. Mengqi Wang
   4. Yanyun Mao
   5. Zhiguo Ma

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This valuable study contributes to the field of neuro-glial biology by establishing a direct causal link between astrocytic metabolism (glycolysis) and the structural wiring of neural circuits. Connecting the metabolic-synaptic mechanism to locomotor reorientation in the dopaminergic circuit offers new insights into how energy metabolism shapes circuit assembly and function. The evidence offers a solid foundation, moving logically from molecular mechanisms to circuit-level anatomy and finally to behavior; however, several central conclusions currently exceed the direct evidence presented. With appropriate calibration of claims and interpretations and/or additional clarifying experiments, the manuscript has the potential to make a significant contribution to our understanding of glial regulation of circuit assembly.

   Reviewed by eLife

4. A single-cell transcriptomic atlas of inner ear morphogenesis in zebrafish

   This article has 9 authors:

   1. Akankshi Munjal
   2. Kalki Kukreja
   3. Samara Williams
   4. Toru Kawanishi
   5. Natasha M O’Brown
   6. Kana Ishimatsu
   7. Allon Klein
   8. Sean G Tsung-Megason
   9. Ian A Swinburne

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This is a valuable study on single-cell transcriptomic analyses, focused on morphogenesis of the zebrafish inner ear in wildtype and lmx1bb mutants. The supporting evidence is mostly convincing, but incomplete in parts.

   Reviewed by eLife

5. Functional definition of the Drosophila airway progenitor field through overlapping compensatory regulators

   This article has 4 authors:

   1. Ryo Matsuda
   2. Chie Hosono
   3. Kaoru Saigo
   4. Christos Samakovlis

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     In this important manuscript, Matsuda and colleagues present a model describing the regulation of tracheal tubulogenesis in Drosophila melanogaster embryos. The authors support this model using convincing approaches that combine novel experimental results with previously published work from their group. While some conclusions are consistent with earlier studies, the present manuscript introduces distinct molecular markers not previously reported, which reinforce the authors' prior findings. In addition, the manuscript analyses, using experimental strategies, the requirement of the Dpp and EGFR signalling pathways for the maintenance of trachealess (trh), one of the key transcription factors governing tracheal development.

   Reviewed by eLife

6. Defining the Antigenic Topology and Prospective Binding Breadth of Vaccination-induced SARS-CoV-2 Neutralizing Antibodies

   This article has 8 authors:

   1. Deepika Jaiswal
   2. Clara G Altomare
   3. Daniel C Adelsberg
   4. Iden A Sapse
   5. Florian Krammer
   6. Viviana Simon
   7. Ali H Ellebedy
   8. Goran Bajic

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This manuscript provides valuable high-resolution structural insights into the interaction between vaccine-elicited antibodies and SARS‑CoV‑2 evolution. The evidence is solid; however, the conclusions could be strengthened with further experimentation and analysis.

   Reviewed by eLife

7. A stress-responsive morphogenetic program of the uterine epithelium safeguards the establishment of early pregnancy

   This article has 9 authors:

   1. Chihiro Ishizawa
   2. Shizu Aikawa
   3. Yamato Fukui
   4. Xueting He
   5. Ryoko Shimizu-Hirota
   6. Daiki Hiratsuka
   7. Mitsunori Matsuo
   8. Takehiro Hiraoka
   9. Yasushi Hirota

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This valuable study reports the architectural reorganization of the uterine luminal epithelium during the implantation period. The data presented are solid, although improvements are needed. This work is of interest to reproductive biologists and physicians practicing reproductive medicine.

   Reviewed by eLife

8. Calibrated analysis framework for nanopore direct RNA sequencing uncovers cell-specific m6A stoichiometry at conserved sites

   This article has 10 authors:

   1. Denise Ohnezeit
   2. Elene Loliashvili
   3. Gregory Putzel
   4. Ruth Verstraten
   5. Jianheng Liu
   6. Luke S Nicholson
   7. Alejandro Pironti
   8. Samie R Jaffrey
   9. Daniel P Depledge
   10. Angus C Wilson

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This valuable study compares orthogonal approaches for detecting RNA chemical modifications and provides a helpful framework for improving the reliability of direct RNA sequencing-based identification of RNA modifications. The evidence supporting the technical benchmarking claims is solid. However, support for the broader biological conclusions is not as strong, and the quantitative interpretation of the results, as well as the limitations of the underlying models, would benefit from further clarification.

   Reviewed by eLife

9. Selective suppression and biasing of chemokine receptors CCR9 and ACKR4 through targeting CCL25 with de novo miniproteins

   This article has 9 authors:

   1. Bas de Boer
   2. Thomas D Lamme
   3. Karlijn Verdwaald
   4. Sara Santamaria Medina
   5. Csongor G Németh
   6. Elisabeth M Elfrink
   7. Martine J Smit
   8. Iwan JP de Esch
   9. Christopher T Schafer

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This important study describes computationally designed proteins that bind to the chemokine CCL25. The authors present evidence that some binders simply prevent chemokine binding to the CCR9 receptor, while one binder changes the downstream signaling triggered by chemokine binding. The evidence is solid overall, but some uncertainty remains with respect to functional selectivity due to sensitivity differences between functional assays and the degree of binder selectivity between the large family of chemokine ligands.

   Reviewed by eLife

10. Programmed Delayed Splicing: A Mechanism for Timed Inflammatory Gene Expression

    This article has 14 authors:

    1. Jacob S Dearborn
    2. Luke Frankiw
    3. Damas W Limoge
    4. Christian H Burns
    5. Logan Vlach
    6. Patricia Turpin
    7. Tylar Kirch
    8. Zachary D Miller
    9. William Dowell
    10. Sylvester Languon
    11. Yvette Garcia-Flores
    12. Robert C Cockrell
    13. David Baltimore
    14. Devdoot Majumdar

    This article has been curated by 1 group:

    • Curated by eLife

      eLife Assessment

      This study analyzes the temporal dynamics of gene expression following TNF stimulation in macrophages. The work brings valuable data and new methodological approaches to implicate the splicing rate of certain introns as a mechanism regulating mature mRNA expression. This will be of interest to audiences in RNA biology and innate immune response regulation. The experimental design is solid for the core findings, although in places the data limit the conclusions.

    Reviewed by eLife