eLife

Latest preprint reviews

1. Syntaxin11 Deficiency Inhibits CRAC Channel Priming to Suppress Cytotoxicity and Gene Expression in T Lymphocytes

   This article has 17 authors:

   1. Sritama Datta
   2. Abhikarsh Gupta
   3. Kunal Mukesh Jagetiya
   4. Resmi Bera
   5. Vikas Tiwari
   6. Atharva Rahul Yande
   7. Megumi Yamashita
   8. Abdul Rishad
   9. Vishal Malik
   10. Sreejith Raran-Kurussi
   11. Sandra Ammann
   12. Mohammad Shahrooei
   13. Kalyaneswar Mandal
   14. Ramanathan Sowdhamini
   15. Murali Prakriya
   16. Adish Dani
   17. Monika Vig

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study reports a novel function for syntaxin 11, a specialized SNARE protein critical for the immune system whose mutations cause familial hemophagocytic lymphohistiocytosis type 4. The data convincingly show that depletion of STX11 impairs store-operated calcium entry in Jurkat T cells and that this defect is recapitulated in primary cells from a patient suffering from the disease; the authors further show that the syntaxin interacts with the pore subunit of the ORAI1 channel and propose that it primes the channel by promoting the assembly of multimers before activation by its endogenous ligand, the ER Ca2+ sensing protein STIM1. This is a conceptually important claim that challenges the prevailing view that all structural transitions in ORAI1 are STIM-driven. The data are high-quality and broadly consistent with the interpretation, but alternative mechanisms for the defects are not considered; additional work should rule out vesicular trafficking, discuss other mechanisms, and address methodological issues.

   Reviewed by eLife

2. Mitochondrial metabolic remodeling drives innate immune activation in Drosophila hemocytes

   This article has 8 authors:

   1. Daewon Lee
   2. Ferdinand Koranteng
   3. Nuri Cha
   4. Sangho Yoon
   5. Kyung-Tae Lee
   6. Jin-Wu Nam
   7. Young V. Kwon
   8. Jiwon Shim

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This important study provides detailed insights into the metabolic states of hemocyte populations across developmental stages and in both physiological and pathological contexts, including during immune challenge. The study provides convincing evidence by comparing the relative utilization of glycolysis and oxidative phosphorylation in Drosophila larval immune cells, and can have implications for metabolic programs that shape immune function in health and disease.

   Reviewed by eLife

3. An engineered multi-step differentiation program in Escherichia coli for self-organized spatial patterning

   This article has 7 authors:

   1. Emanuele Boni
   2. Hélène Siboulet
   3. Giacomo Ceracchini
   4. Içvara Aor
   5. Gábor Holló
   6. Hadiastri Kusumawardhani
   7. Yolanda Schaerli

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This valuable study demonstrates that a multi-step differentiation programme in bacteria combining a bistable switch with two quorum-sensing systems is capable of generating autonomous and self-organized spatial patterns. The evidence for the core engineering system supporting patterning across several conditions is convincing, albeit incomplete for the stronger differentiation/maturation claims because the irreversibility of the proposed states is not consistently established, and some modelling and conceptual interpretation details require further clarification.

   Reviewed by eLife

4. Transitory enhancement of GATA2 chromatin engagement during early erythroid differentiation

   This article has 7 authors:

   1. John W Hobbs
   2. Samuel J. Taylor
   3. Rajni Kumari
   4. Nayem Haque
   5. Lou Lou Victor
   6. Ulrich Steidl
   7. Robert A. Coleman

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This important study combines single-molecule imaging and CUT&TAG to address the molecular mechanism underlying the differentiation process that initiates the formation of red blood cells in the bone marrow. The authors provide evidence that the transcription factor GATA2 transiently associates with a new set of genomic loci early in the differentiation process before it is replaced by GATA1. Together, the experiments across three biological systems are solid, but they could benefit from additional details and controls to strengthen the conclusions.

   Reviewed by eLife

5. Functional Separation of mRNA Domains Coordinates Pluripotent Cell Behavior

   This article has 9 authors:

   1. Ze Yang
   2. Shaoyi Ji
   3. Kristina Ivanov
   4. Priyanka Kadav
   5. Mengmeng Song
   6. Leonardi Gozali
   7. Sophie Parsa
   8. Barry Behr
   9. Mary Hynes

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study provides fundamental insights by demonstrating that the Nanog mRNA coding sequence (CDS) and 3′UTR domains are spatially segregated and functionally distinct in pluripotent stem cells and blastocysts, with 3′UTR-enriched border cells primarily influencing morphogenesis and CDS-enriched inner cells largely regulating transcription and epigenetic programs. The work opens a novel conceptual avenue for understanding how separable mRNA domains can differentially control cell behavior and differentiation. However, the evidence is incomplete, as key aspects of the molecular nature, biogenesis, and precise characterization of the separated 3′UTR and CDS RNA species, as well as causal links between their perturbation and the observed phenotypes (e.g., via rescue and deeper characterization of 3′UTR elements), remain to be fully established.

   Reviewed by eLife

6. Simplified model of intrinsically bursting neurons

   This article has 3 authors:

   1. Nikhil X Bhattasali
   2. Lerrel Pinto
   3. Grace W Lindsay

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     The authors propose a "simplified" model for intrinsically bursting neurons with explicitly controllable parameterization of oscillatory dynamics. The evidence that the modeling approach is generally appropriate and practical for modeling rhythmic bursting neurons and neural circuits is currently incomplete. Based on what the authors present, this model appears to have limited neurobiological relevance and utility but may be useful as a controller for an artificial system, such as in neuro-robotics applications.

   Reviewed by eLife

7. Fusion Loop Modified and Mature Dengue Virus Elicits Protective Serum with Minimal Antibody Dependent Enhancement

   This article has 6 authors:

   1. Yago R Dalben
   2. Jacob J Adler
   3. Rita M Meganck
   4. Katelyn Duenas
   5. Lisa J Snoderly-Foster
   6. Longping V Tse

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     There is a need for better and safer dengue virus live attenuated vaccines. This manuscript describes important findings that could lead to the design of a strongly immunogenic, tetravalent live attenuated vaccine for dengue, without the risk of causing antibody-dependent enhancement. However, the experimental evidence presented is incomplete since only constructions of one serotype were tested to prove the principle.

   Reviewed by eLife

8. Macrophages drive inguinal fat pad and lymph node remodelling in response to peripheral inflammation

   This article has 10 authors:

   1. Robin Bartolini
   2. Deepika Sharma
   3. Gillian J Wilson
   4. Gillian Dunphy
   5. Jonathan Cavanagh
   6. Heba A Halawa
   7. John Cole
   8. Stefan Weidt
   9. Kirstyn Gardner-Stephen
   10. Gerard J Graham

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study presents potentially important findings linking peripheral inflammation to the remodeling of perinodal adipose tissue and draining lymph nodes, suggesting a mechanism by which local tissue inflammation can reshape LN structure and metabolism. The idea is solid and supported by observations. However, the evidence remains incomplete in parts, as several conclusions rely on correlative weight and cellularity measurements, and macrophage involvement requires further validation.

   Reviewed by eLife

9. Peristaltic contractions drive gut anisotropic growth through collective cell rearrangements

   This article has 3 authors:

   1. Koji Kawamura
   2. Yoshiko Takahashi
   3. Masafumi Inaba

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This is a useful study investigating the role of peristalsis in the elongation of the gut, using the chick ceca as a model. The work employs optogenetics together with embryological approaches to establish links between peristaltic muscle contractions and downstream cell behaviors that lead to tube elongation. However, the work is somewhat incomplete, limited in mechanistic insights that would extend beyond prior work in the literature, which has already suggested a role for smooth muscle contractility in avian gut elongation.

   Reviewed by eLife

10. Tatton-Brown-Rahman Syndrome-associated DNMT3A mutations de-repress cortical interneuron differentiation to disrupt neuronal network function

    This article has 11 authors:

    1. Gareth Chapman
    2. Julianna J. Determan
    3. John R. Edwards
    4. Faiza Batool
    5. James E. Huettner
    6. Ramachandran Prakasam
    7. Sydney R. Crump
    8. Travis E. Law
    9. Haley Jetter
    10. Harrison W. Gabel
    11. Kristen L. Kroll

    This article has been curated by 1 group:

    • Curated by eLife

      eLife Assessment

      This is an important study that develops multiple human iPSC-based models to study the consequences of DNMT3A mutations in Tatton-Brown-Rahman Syndrome. Convincing evidence shows dysregulation of GABAergic interneuron development and function, and the authors identify some of the key signaling mechanisms underlying these changes. This study will be of interest for understanding the functions of DNMT3A in brain development and the causes of neurological dysfunction in Tatton-Brown-Rahman Syndrome.

    Reviewed by eLife