eLife

Latest preprint reviews

1. Nerve Injury-Induced Protein 2 preserves lysosomal membrane integrity to suppress ferroptosis

   This article has 5 authors:

   1. Jin Zhang
   2. Miranda Bustamante
   3. Yang Shi
   4. Ken-ichi Nakajima
   5. Xinbin Chen

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This fundamental work uncovers an unexpected lysosomal function for NINJ2 and links it to ferroptosis and cancer biology. The evidence supporting the conclusions appears to be convincing. Additional mechanistic clarification, particularly around the NINJ2-LAMP1 interaction and ferroptosis specificity, will further strengthen the manuscript. This work will be of general interest to the community of ferroptosis and cancer biology.

   Reviewed by eLife

2. Dynamic RNA Polymerase compartments organize the transcription of gene clusters

   This article has 3 authors:

   1. Yi-Hui Wang
   2. Hannah L Hertz
   3. Wen Tang

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This manuscript explores the dynamic behaviors of Pol II and Pol III puncta that encompass the SL1 and 5S genes, following up on the authors' prior studies on ATTF-6. The authors show that ATTF-6 is required for RNA Pol II but not RNA Pol III foci, demonstrating that within the gene cluster, the regulation of RNA Pol II and RNA Pol III remain distinct from each other. The study is useful for analyzing understudied gene families, but it is incomplete and needs additional edits and experiments.

   Reviewed by eLife

3. Capturing instantaneous neural signal-behavior relationships with concurrent functional mixed models

   This article has 4 authors:

   1. Al W Xin
   2. Erjia Cui
   3. Francisco Pereira
   4. Gabriel Loewinger

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This valuable work extends a previously published regression framework for trial-aligned photometry data incorporating functional variables. However, the evidence is generally incomplete, due to the way that within-trial changes in variables have been incorporated into an inherently cross-trial analysis framework, which will limit general adoption. The ideas in this work will be of interest to researchers analyzing photometry signals.

   Reviewed by eLife

4. Effects of knockdown of autophagy pathway genes on C. elegans longevity are highly condition dependent

   This article has 6 authors:

   1. Kuei Ching Hsiung
   2. Hannah Chapman
   3. Xiaoya Wei
   4. Xiaoyu Sun
   5. Isadora Rawlinson
   6. David Gems

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study presents a valuable finding on the condition dependence of autophagy-mediated lifespan regulation in C. elegans. The evidence is solid, as the data broadly support the main claims, although variability between biological replicates and limited mechanistic exploration leave some conclusions less firmly established. The work will be of interest to researchers studying autophagy, ageing, and intracellular trafficking.

   Reviewed by eLife

5. No evidence for disassortative mating based on HLA in a small-scale, endogamous population

   This article has 12 authors:

   1. Gillian L. Meeks
   2. Brooke Scelza
   3. Katherine. M. Kichula
   4. Catrinel Berevoescu
   5. Kristin Hardy
   6. Ticiana D.J. Farias
   7. Genelle F. Harrison
   8. Nicholas R. Pollock
   9. Neus Font-Porterias
   10. Sean Prall
   11. Paul J. Norman
   12. Brenna M. Henn

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This important study addresses the long-debated hypothesis that humans preferentially choose partners with dissimilar immune genes, using data from a small-scale society that allows comparison between arranged and self-chosen partnerships. Across multiple analyses controlling for genome-wide relatedness and examining functional immune diversity, the authors find no evidence of HLA/MHC-based (dis)assortative mating, suggesting that immune gene variation has limited influence on mate choice in this relatively homogeneous population and that the observed patterns instead reflect selection acting directly on immune loci. While the strength of the evidence is compelling for this population, several conclusions rely on indirect reconstruction methods and imputed data for a very complex region of the genome, which may limit how firmly some claims can be supported.

   Reviewed by eLife

6. ANTIPODE Provides a Global View of Cell Type Homology and Transcriptomic Divergence in the Developing Mammalian Brain

   This article has 10 authors:

   1. Matthew T Schmitz
   2. Jingwen W Ding
   3. Sara Nolbrant
   4. Reed McMullen
   5. Chang N Kim
   6. Bryan J Pavlovic
   7. Tomasz J Nowakowski
   8. Trygve E Bakken
   9. Chun Jimmie Ye
   10. Alex A Pollen

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This valuable study is an approach to integrating and comparing single-cell genomics data across species. The evidence supporting the conclusions of this work is solid, and ANTIPODE presents an updated methodological approach to determining how gene expression at the cell-type level has evolved. Thus, ANTIPODE should provide broad utility to studies of comparative neurogenomics and be of use to neuroscientists and evolutionary biologists.

   Reviewed by eLife

7. Synergistic effects of deleting the tyrosine phosphatases Shp1 and Shp2 on megakaryopoiesis and thrombopoiesis in mice

   This article has 13 authors:

   1. Elsa Barré
   2. Marc-Damien Lourenco-Rodrigues
   3. Lucie Zimmermann
   4. Marion Pugliano
   5. Cécile Loubière
   6. Fabienne Proamer
   7. Jean-Yves Rinckel
   8. Anita Eckly
   9. Zihan Qu
   10. Jinmin Miao
   11. Zhong-Yin Zhang
   12. Yotis A Senis
   13. Alexandra Mazharian

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This manuscript provides an important contribution to the field of platelet biogenesis, and the convincing evidence will advance our understanding of signal transduction driving the development of late megakaryopoiesis and platelet reactivity that results in bleeding diathesis. The paper is noteworthy for analyzing two related, either singly or in combination, tyrosine phosphatases in this conditional, stage development gene knockout. Because SHP1 is a negative regulator and SHP2 is an activator, the synergistic effects found in the double knockout were surprising.

   Reviewed by eLife

8. A minimal tooth enhancer regulates dlx2b expression during zebrafish tooth formation: insights into cis-regulatory logic in organogenesis

   This article has 12 authors:

   1. William R Jackman
   2. Yujin Moon
   3. Carol K Cox
   4. Drew R Anderson
   5. Audrey A DeFusco
   6. Vy M Nguyen
   7. Sarah Y Liu
   8. Elisabeth H Carter
   9. Hana E Littleford
   10. Elizabeth K Richards
   11. Andrea L Jowdry
   12. Yann Gibert

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study provides valuable findings in the study of enhancer biology by identifying and dissecting a minimal enhancer regulating dlx2b expression during zebrafish tooth development, supported by promoter dissection, reporter assays, and genome-editing approaches. The work offers a resource and extends previous findings but has limited broader impact, with several conclusions about general cis-regulatory principles and functional consequences remaining only partially supported. Accordingly, the strength of evidence is at present incomplete, as additional functional validation would be needed to fully substantiate some of the claims.

   Reviewed by eLife

9. Continuous partitioning of neuronal variability

   This article has 3 authors:

   1. Anuththara Rupasinghe
   2. Adam S Charles
   3. Jonathan W Pillow

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This work of fundamental significance introduces a novel statistical model of spiking activity that incorporates continuous-time gain modulation. The authors provide exceptional evidence that the model outperforms earlier approaches and alternative candidates in capturing spiking responses across multiple visual areas in the macaque. Beyond its methodological contribution, the study offers new insights into how stimulus-driven variability and internally generated gain fluctuations evolve over time and between brain areas. The framework is likely to find broad application beyond the datasets examined here.

   Reviewed by eLife

10. Identity and functions of monoaminergic neurons in the predatory nematode Pristionchus pacificus reveal nervous system conservation and divergence

    This article has 15 authors:

    1. Curtis M Loer
    2. Hyunsoo Yim
    3. Luke T Geiger
    4. Yasmin H Ramadan
    5. Megan F Hampton
    6. Diana V Bernal
    7. Heather R Carstensen
    8. Jorge Morgan
    9. Laura Rivard
    10. Theresa Medina
    11. Steven J Cook
    12. Misako Okumura
    13. James Lightfoot
    14. Oliver Hobert
    15. Ray L Hong

    This article has been curated by 1 group:

    • Curated by eLife

      eLife Assessment

      This study presents valuable insights into cellular sites of monoamine production and presence in Pristionchus pacificus, providing a comparative reference for the detailed knowledge of C. elegans, as well as using this information to compare serotonergic anatomy in 22 nematode species. Functional assays support evolved differences in monoaminergic control over certain, but not all, tested behaviors. The evidence is convincing, combining careful genetic experiments and comparative analysis that are well aligned with the conclusions. The results will serve as a basis for (comparative) structural-functional studies of nematode behavior.

    Reviewed by eLife