Neuroscience

Nociceptors use multiple neurotransmitters to drive pain

1. Donald Iain MacDonald
2. Rakshita Balaji
3. Alexander T. Chesler

Reviewed by PREreview

Fasting and Caloric Restriction Activate an ADIOL ‐ NHR ‐91‐Kynurenine Pathway Signaling Axis to Promote Healthspan

1. Ana Guijarro‐Hernández
2. Shinja Yoo
3. George A. Lemieux
4. Sena Komatsu
5. Abdullah Q. Latiff
6. Rishika R. Patil
7. Kaveh Ashrafi

Reviewed by PREreview

The encoding of interoceptive-based predictions by the paraventricular nucleus of the thalamus D2R+ neurons

1. Briana Machen
2. Sierra N. Miller
3. Al Xin
4. Carine Lampert
5. Lauren Assaf
6. Julia Tucker
7. Sarah Herrell
8. Francisco Pereira
9. Gabriel Loewinger
10. Sofia Beas

Reviewed by PREreview

Emergence of Functional Heart-Brain Circuits in a Vertebrate

1. Luis Hernandez-Nunez
2. Joana Avrami
3. Sky Shi
4. Areni Markarian
5. Annette Kim
6. Jonathan Boulanger-Weill
7. Virginia Rutten
8. Arman Zarghani-Shiraz
9. Misha B Ahrens
10. Florian Engert
11. Mark C Fishman

• Curated by eLife

  eLife Assessment

  In this important manuscript, the authors establish a vertebrate model for studying the development of circuits that control heart rate. This contribution uses a combination of experimental techniques to provide compelling information for scientists looking to understand how heart rate regulation emerges during development.

Reviewed by eLife

Intersection of transient cell states with stable cell types in hippocampus

1. Jack A Olmstead
2. Lauren E King
3. Brenda L Bloodgood

• Curated by eLife

  eLife Assessment

  This important study provides a detailed analysis of the transcriptional landscape of the mouse hippocampus in the context of various physiological states. The main conclusions have solid support: that most transcriptional targets are generally stable, with notable exceptions in the dentate gyrus and with regard to circadian changes. There are some weaknesses and it would improve the manuscript to address them.

Reviewed by eLife

Voltage imaging reveals the emergence of population activity in the spinal cord

1. Asuka Shiraishi
2. Ayane Hayashi
3. Narumi Fukuda
4. Mari Hishinuma
5. Hiroaki Miyazawa
6. Sachiko Tsuda

• Curated by eLife

  eLife Assessment

  This paper presents an important advance in genetically encoded voltage imaging of the developing zebrafish spinal cord in vivo, capturing voltage dynamics in neuronal populations, single cells, and subcellular compartments inaccessible to patch clamp, and diverse spike waveforms and subthreshold voltage dynamics inaccessible to calcium imaging. The work identifies a developmental progression from irregular voltage fluctuations to coordinated contralateral and ipsilateral activity, providing insight into how electrical dynamics and cellular morphology evolve during circuit formation. The strength of evidence is solid, with imaging data supporting the main conclusions, although the manuscript would be strengthened by more complete methodological documentation and clearer context relative to earlier calcium imaging studies. Overall, this study provides a resource that is of importance for researchers investigating neural development and circuit assembly, illustrating the value of voltage imaging as a general tool for probing bioelectric mechanisms in morphogenesis and circuit development.

Reviewed by eLife

Maximized field-of-view deep-brain calcium imaging through gradient-index lenses

1. Chenmao Wang
2. Zongyue Cheng
3. Yuting Li
4. Jianian Lin
5. Meng Cui

• Curated by eLife

  eLife Assessment

  This study presents a valuable and practical approach for one-photon imaging through GRIN lenses. By scanning a low numerical aperture (NA) beam and collecting fluorescence with a high NA, the method expands the usable field of view and yields clearer cellular signals. The evidence is solid overall, with strong qualitative demonstrations, but some claims would benefit from additional quantitative tests. The work will interest researchers who need simple, scalable tools for large‑area cellular imaging in the brain.

Reviewed by eLife

Insulin resistance alters cortical inhibitory neurons and microglia to exacerbate Alzheimer’s knock-in mouse phenotypes

1. LaShae Nicholson
2. Si Jie Tang
3. Tejaswini Karra
4. Habiba Abouelatta
5. Stephen M. Strittmatter

Reviewed by PREreview