Molecular Biology

Influence of viral transport media and freeze-thaw cycling on the sensitivity of qRT-PCR detection of SARS-CoV-2 nucleic acids

1. Cian Holohan
2. Sophia Hanarahan
3. Nathan Feely
4. Peng Li
5. John O’Connell
6. Catherine Moss
7. Michael Carr
8. Oya Tagit
9. Gil U Lee

Reviewed by ScreenIT

Molecular Dynamics Analysis of Fast-Spreading Severe Acute Respiratory Syndrome Coronavirus 2 Variants and Their Effects on the Interaction with Human Angiotensin-Converting Enzyme 2

1. Anacleto Silva de Souza
2. Vitor Martins de Freitas Amorim
3. Gabriela D. A. Guardia
4. Felipe R. C. dos Santos
5. Filipe F. dos Santos
6. Robson Francisco de Souza
7. Guilherme de Araujo Juvenal
8. Yihua Huang
9. Pingju Ge
10. Yinan Jiang
11. Coco Li
12. Prajwal Paudel
13. Henning Ulrich
14. Pedro A. F. Galante
15. Cristiane Rodrigues Guzzo

Reviewed by ScreenIT

Double-stranded RNA drives SARS-CoV-2 nucleocapsid protein to undergo phase separation at specific temperatures

1. Christine A Roden
2. Yifan Dai
3. Catherine A Giannetti
4. Ian Seim
5. Myungwoon Lee
6. Rachel Sealfon
7. Grace A McLaughlin
8. Mark A Boerneke
9. Christiane Iserman
10. Samuel A Wey
11. Joanne L Ekena
12. Olga G Troyanskaya
13. Kevin M Weeks
14. Lingchong You
15. Ashutosh Chilkoti
16. Amy S Gladfelter

Reviewed by ScreenIT

In vitro evaluation of the effect of mutations in primer binding sites on detection of SARS-CoV-2 by RT-qPCR

1. Fee Zimmermann
2. Maria Urban
3. Christian Krüger
4. Mathias Walter
5. Roman Wölfel
6. Katrin Zwirglmaier

Reviewed by ScreenIT

Irradiation of UVC LED at 277 nm inactivates coronaviruses by photodegradation of spike protein

1. Qunxiang Ong
2. J. W. Ronnie Teo
3. Joshua Dela Cruz
4. Elijah Wee
5. Winson Wee
6. Weiping Han

Reviewed by ScreenIT

MINERVA: A Facile Strategy for SARS-CoV-2 Whole-Genome Deep Sequencing of Clinical Samples

1. Chen Chen
2. Jizhou Li
3. Lin Di
4. Qiuyu Jing
5. Pengcheng Du
6. Chuan Song
7. Jiarui Li
8. Qiong Li
9. Yunlong Cao
10. X. Sunney Xie
11. Angela R. Wu
12. Hui Zeng
13. Yanyi Huang
14. Jianbin Wang

Reviewed by ScreenIT, preLights

A twin UGUA motif directs the balance between gene isoforms through CFIm and the mTORC1 signaling pathway

1. R Samuel Herron
2. Alexander K Kunisky
3. Jessica R Madden
4. Vivian I Anyaeche
5. May Z Maung
6. Hun-Way Hwang

• Curated by eLife

  eLife assessment

  In this valuable report, the authors explore the connection between mTORC signaling and APA regulated by the CFIm complex. Using a combination of genetic and functional genomics approaches, the study reveals that a twin UGUA motif is a potent cis acting regulation of PAS usage that is recognized by CFIm. Overall, the evidence in general is convincing and supports the conclusions and provides the field with additional mechanistic insight of how signaling connects to APA regulatory machinery.

Reviewed by eLife

The short isoform of the host antiviral protein ZAP acts as an inhibitor of SARS-CoV-2 programmed ribosomal frameshifting

1. Matthias M. Zimmer
2. Anuja Kibe
3. Ulfert Rand
4. Lukas Pekarek
5. Liqing Ye
6. Stefan Buck
7. Redmond P. Smyth
8. Luka Cicin-Sain
9. Neva Caliskan

Reviewed by ScreenIT

The N-terminal domain of SARS-CoV-2 nsp1 plays key roles in suppression of cellular gene expression and preservation of viral gene expression

1. Aaron S. Mendez
2. Michael Ly
3. Angélica M. González-Sánchez
4. Ella Hartenian
5. Nicholas T. Ingolia
6. Jamie H. Cate
7. Britt A. Glaunsinger

Reviewed by ScreenIT

Long non-coding RNA Neat1 and paraspeckle components are translational regulators in hypoxia

1. Anne-Claire Godet
2. Emilie Roussel
3. Florian David
4. Fransky Hantelys
5. Florent Morfoisse
6. Joffrey Alves
7. Françoise Pujol
8. Isabelle Ader
9. Edouard Bertrand
10. Odile Burlet-Schiltz
11. Carine Froment
12. Anthony K Henras
13. Patrice Vitali
14. Eric Lacazette
15. Florence Tatin
16. Barbara Garmy-Susini
17. Anne-Catherine Prats

• Curated by eLife

  Evaluation Summary:

  This study reports a novel link by which specific cellular mRNAs, that contain internal ribosome sites (IRES), are made competent for translation in paraspeckles in the nucleus. The data showed that a long noncoding RNA, Neat1, is the major player to add transacting factors to the internal ribosome entry site located in fibroblast growth factor 1 mRNAs in the nucleus. This event occurs during hypoxia in mouse cardiomyocytes and is, thus, relevant to gene expression during angiogenesis.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife