Molecular Biology

Inducible and reversible inhibition of miRNA-mediated gene repression in vivo

1. Gaspare La Rocca
2. Bryan King
3. Bing Shui
4. Xiaoyi Li
5. Minsi Zhang
6. Kemal M Akat
7. Paul Ogrodowski
8. Chiara Mastroleo
9. Kevin Chen
10. Vincenzo Cavalieri
11. Yilun Ma
12. Viviana Anelli
13. Doron Betel
14. Joana Vidigal
15. Thomas Tuschl
16. Gunter Meister
17. Craig B Thompson
18. Tullia Lindsten
19. Kevin Haigis
20. Andrea Ventura

• Curated by eLife

  Evaluation Summary:

  MicroRNAs (miRNAs) control gene expression during development and play crucial roles in disease. Loss-of-function mutations in key pathway components are embryonically lethal. Here, La Rocca et al. establish an elegant mouse model that enables acute and reversible inhibition of miRNA-guided silencing. Analysis of this model has convincingly demonstrated that miRNA activity is dispensable for homeostasis in most adult tissues, with the notable exception of heart and skeletal muscle. This work provides an extremely useful tool for the study of miRNAs in vivo and provides new insights into the roles of miRNAs in adult mammalian tissues. The findings presented will impact many fields given the well-established roles of miRNAs in normal development and diseases.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their names with the authors.)

Reviewed by eLife

Single-molecule imaging of chromatin remodelers reveals role of ATPase in promoting fast kinetics of target search and dissociation from chromatin

1. Jee Min Kim
2. Pat Visanpattanasin
3. Vivian Jou
4. Sheng Liu
5. Xiaona Tang
6. Qinsi Zheng
7. Kai Yu Li
8. Jonathan Snedeker
9. Luke D Lavis
10. Timothee Lionnet
11. Carl Wu

• Curated by eLife

  Evaluation Summary:

  In this study, Kim and co-workers track the dynamics of a large set of different ATP-dependent chromatin remodelers in living cells by utilizing state-of-the-art single-molecule imaging. They report that the remodelers exhibit very high turnover rates at target loci/nucleosomes, find evidence for cooperativity among the remodelers, and reveal the role of ATP hydrolysis in those interactions. These observations allow the authors to put forward a model for tug-of-war activities that modulate the accessibility of promoter regions for transcriptional activity. This manuscript brings important new information to the remodeler and chromatin dynamics field.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their names with the authors.)

Reviewed by eLife

Ribosome slowdown triggers codon‐mediated mRNA decay independently of ribosome quality control

1. Yuichiro Mishima
2. Peixun Han
3. Kota Ishibashi
4. Seisuke Kimura
5. Shintaro Iwasaki

Reviewed by Review Commons

Monitoring the spread of the SARS-CoV-2 (Coronaviridae: Coronavirinae: Betacoronavirus; Sarbecovirus) variants in the Moscow region using targeted high-throughput sequencing

1. N. I. Borisova
2. I. A. Kotov
3. A. A. Kolesnikov
4. V. V. Kaptelova
5. A. S. Speranskaya
6. L. Yu. Kondrasheva
7. E. V. Tivanova
8. K. F. Khafizov
9. V. G. Akimkin

Reviewed by ScreenIT

Conversion rate to the secondary conformation state in the binding mode of SARS-CoV-2 spike protein to human ACE2 may predict infectivity efficacy of the underlying virus mutant

1. Marc M. Sevenich
2. Joop van den Heuvel
3. Ian Gering
4. Jeannine Mohrlüder
5. Dieter Willbold

Reviewed by ScreenIT

Receptor-binding domain recombinant protein on alum-CpG induces broad protection against SARS-CoV-2 variants of concern

1. Jeroen Pollet
2. Ulrich Strych
3. Wen-Hsiang Chen
4. Leroy Versteeg
5. Brian Keegan
6. Bin Zhan
7. Junfei Wei
8. Zhuyun Liu
9. Jungsoon Lee
10. Rahki Kundu
11. Rakesh Adhikari
12. Cristina Poveda
13. Maria Jose Villar
14. Syamala Rani Thimmiraju
15. Brianna Lopez
16. Portia M. Gillespie
17. Shannon Ronca
18. Jason T. Kimata
19. Martin Reers
20. Vikram Paradkar
21. Peter J. Hotez
22. Maria Elena Bottazzi

Reviewed by ScreenIT

Mouse Antibodies with Activity Against the SARS-CoV-2 D614G and B.1.351 Variants

1. Larisa Troitskaya
2. Nelson Lap Shun Chan
3. Brendon Frank
4. Daniel J. Capon
5. Brian A. Zabel
6. Xiaomei Ge
7. Dan Luo
8. Rachel Martinelli
9. Jing Jin
10. Graham Simmons

Reviewed by ScreenIT

SARS-CoV-2 Nsp14 mediates the effects of viral infection on the host cell transcriptome

1. Michela Zaffagni
2. Jenna M Harris
3. Ines L Patop
4. Nagarjuna Reddy Pamudurti
5. Sinead Nguyen
6. Sebastian Kadener

• Curated by eLife

  Evaluation Summary:

  The paper makes the provocative claim that SARS-CoV-2 Nsp14 is the key protein that mediates the effects of viral infection on the host cell transcriptome. The current evidence for this claim is good, but the paper would benefit from a few additional experiments. If confirmed by these experiments, the conclusion is unexpected and important, especially in these COVID times.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their name with the authors.)

Reviewed by eLife, ScreenIT

circFL-seq reveals full-length circular RNAs with rolling circular reverse transcription and nanopore sequencing

1. Zelin Liu
2. Changyu Tao
3. Shiwei Li
4. Minghao Du
5. Yongtai Bai
6. Xueyan Hu
7. Yu Li
8. Jian Chen
9. Ence Yang

• Curated by eLife

  Evaluation Summary:

  This study provides an experimental and computational method for the identification and reconstruction of full-length circRNAs using nanopore sequencing,. With a better comparison to other existing methods in the field and a clearer demonstration of the advantages of the described methodology, the work would be of great interest to researchers in the circular RNA community.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #3 agreed to share their name with the authors.)

Reviewed by eLife

N4-hydroxycytidine and inhibitors of dihydroorotate dehydrogenase synergistically suppress SARS-CoV-2 replication

1. Kim M. Stegmann
2. Antje Dickmanns
3. Natalie Heinen
4. Uwe Groß
5. Dirk Görlich
6. Stephanie Pfaender
7. Matthias Dobbelstein

Reviewed by ScreenIT