Molecular Biology

Site-specific recognition of SARS-CoV-2 nsp1 protein with a tailored titanium dioxide nanoparticle – elucidation of the complex structure using NMR data and theoretical calculation

1. Peter Agback
2. Tatiana Agback
3. Francisco Dominguez
4. Elena I. Frolova
5. Gulaim A. Seisenbaeva
6. Vadim G. Kessler

Reviewed by ScreenIT

Improving RT-LAMP detection of SARS-CoV-2 RNA through primer set selection and combination

1. Yinhua Zhang
2. Nathan A. Tanner

Reviewed by ScreenIT

Comparable neutralization of SARS-CoV-2 Delta AY.1 and Delta with individuals sera vaccinated with BBV152

1. Pragya D Yadav
2. Rima R Sahay
3. Gajanan Sapkal
4. Dimpal Nyayanit
5. Anita M Shete
6. Gururaj Deshpande
7. Deepak Y Patil
8. Nivedita Gupta
9. Sanjay Kumar
10. Priya Abraham
11. Samiran Panda
12. Balram Bhargava

Reviewed by ScreenIT

SARS-CoV-2 Fears Green: The Chlorophyll Catabolite Pheophorbide A Is a Potent Antiviral

1. Guillermo H. Jimenez-Aleman
2. Victoria Castro
3. Addis Londaitsbehere
4. Marta Gutierrez-Rodríguez
5. Urtzi Garaigorta
6. Roberto Solano
7. Pablo Gastaminza

Reviewed by ScreenIT

Defining proximity proteomics of post-translationally modified proteins by antibody-mediated protein A-APEX2 labeling

1. Xinran Li
2. Jiaqi Zhou
3. Wenjuan Zhao
4. Qing Wen
5. Weijie Wang
6. Huipai Peng
7. Kelly J. Bouchonville
8. Steven M. Offer
9. Zhiquan Wang
10. Nan Li
11. Haiyun Gan

Reviewed by Review Commons

SARS-CoV-2 Restructures the Host Chromatin Architecture

1. Ruoyu Wang
2. Joo-Hyung Lee
3. Feng Xiong
4. Jieun Kim
5. Lana Al Hasani
6. Xiaoyi Yuan
7. Pooja Shivshankar
8. Joanna Krakowiak
9. Chuangye Qi
10. Yanyu Wang
11. Holger K. Eltzschig
12. Wenbo Li

Reviewed by ScreenIT

In situ imaging of bacterial outer membrane projections and associated protein complexes using electron cryo-tomography

1. Mohammed Kaplan
2. Georges Chreifi
3. Lauren Ann Metskas
4. Janine Liedtke
5. Cecily R Wood
6. Catherine M Oikonomou
7. William J Nicolas
8. Poorna Subramanian
9. Lori A Zacharoff
10. Yuhang Wang
11. Yi-Wei Chang
12. Morgan Beeby
13. Megan J Dobro
14. Yongtao Zhu
15. Mark J McBride
16. Ariane Briegel
17. Carrie L Shaffer
18. Grant J Jensen

Reviewed by Review Commons

Role of BRCA2 DNA-binding and C-terminal domain in its mobility and conformation in DNA repair

1. Maarten W Paul
2. Arshdeep Sidhu
3. Yongxin Liang
4. Sarah E van Rossum-Fikkert
5. Hanny Odijk
6. Alex N Zelensky
7. Roland Kanaar
8. Claire Wyman

• Curated by eLife

  Evaluation Summary:

  This work is of interest to readers in the field of genome stability, DNA repair and associated human diseases. The manuscript describes systematic analyses of the crucial DNA repair mediator BRCA2 and its variants lacking the DNA binding domain or RAD51 interacting C-terminal domain, and the conclusions present a conceptual advance as to how BRCA2 promotes DNA repair. The work is a technical tour de force that includes evaluation of the DNA damage response, gene targeting and single particle tracking in mouse embryonic stem cells, as well as biophysical analyses of the human counterparts. The key claims of the manuscript are largely supported by the data.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their names with the authors.)

Reviewed by eLife

Inducible and reversible inhibition of miRNA-mediated gene repression in vivo

1. Gaspare La Rocca
2. Bryan King
3. Bing Shui
4. Xiaoyi Li
5. Minsi Zhang
6. Kemal M Akat
7. Paul Ogrodowski
8. Chiara Mastroleo
9. Kevin Chen
10. Vincenzo Cavalieri
11. Yilun Ma
12. Viviana Anelli
13. Doron Betel
14. Joana Vidigal
15. Thomas Tuschl
16. Gunter Meister
17. Craig B Thompson
18. Tullia Lindsten
19. Kevin Haigis
20. Andrea Ventura

• Curated by eLife

  Evaluation Summary:

  MicroRNAs (miRNAs) control gene expression during development and play crucial roles in disease. Loss-of-function mutations in key pathway components are embryonically lethal. Here, La Rocca et al. establish an elegant mouse model that enables acute and reversible inhibition of miRNA-guided silencing. Analysis of this model has convincingly demonstrated that miRNA activity is dispensable for homeostasis in most adult tissues, with the notable exception of heart and skeletal muscle. This work provides an extremely useful tool for the study of miRNAs in vivo and provides new insights into the roles of miRNAs in adult mammalian tissues. The findings presented will impact many fields given the well-established roles of miRNAs in normal development and diseases.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their names with the authors.)

Reviewed by eLife

Single-molecule imaging of chromatin remodelers reveals role of ATPase in promoting fast kinetics of target search and dissociation from chromatin

1. Jee Min Kim
2. Pat Visanpattanasin
3. Vivian Jou
4. Sheng Liu
5. Xiaona Tang
6. Qinsi Zheng
7. Kai Yu Li
8. Jonathan Snedeker
9. Luke D Lavis
10. Timothee Lionnet
11. Carl Wu

• Curated by eLife

  Evaluation Summary:

  In this study, Kim and co-workers track the dynamics of a large set of different ATP-dependent chromatin remodelers in living cells by utilizing state-of-the-art single-molecule imaging. They report that the remodelers exhibit very high turnover rates at target loci/nucleosomes, find evidence for cooperativity among the remodelers, and reveal the role of ATP hydrolysis in those interactions. These observations allow the authors to put forward a model for tug-of-war activities that modulate the accessibility of promoter regions for transcriptional activity. This manuscript brings important new information to the remodeler and chromatin dynamics field.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their names with the authors.)

Reviewed by eLife