Molecular Biology

Ssl2/TFIIH function in transcription start site scanning by RNA polymerase II in Saccharomyces cerevisiae

1. Tingting Zhao
2. Irina O Vvedenskaya
3. William KM Lai
4. Shrabani Basu
5. B Franklin Pugh
6. Bryce E Nickels
7. Craig D Kaplan

• Curated by eLife

  Evaluation Summary:

  Kaplan and colleagues build upon their earlier work using genetic phenotypes to find and analyze mutations that determine how mRNA start sites are chosen. Here they provide convincing genetic evidence supporting a model in which the Transcription Factor II H (TFIIH) protein complex pushes downstream DNA back into the RNA polymerase active site, creating a window within which the polymerase can choose particular start sites. This will primarily interest those in the transcription field who are thinking about initiation mechanisms.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

SARS-CoV-2 Delta Variant Pathogenesis and Host Response in Syrian Hamsters

1. Sreelekshmy Mohandas
2. Pragya Dhruv Yadav
3. Anita Shete
4. Dimpal Nyayanit
5. Gajanan Sapkal
6. Kavita Lole
7. Nivedita Gupta

Reviewed by ScreenIT

Site-specific recognition of SARS-CoV-2 nsp1 protein with a tailored titanium dioxide nanoparticle – elucidation of the complex structure using NMR data and theoretical calculation

1. Peter Agback
2. Tatiana Agback
3. Francisco Dominguez
4. Elena I. Frolova
5. Gulaim A. Seisenbaeva
6. Vadim G. Kessler

Reviewed by ScreenIT

Improving RT-LAMP detection of SARS-CoV-2 RNA through primer set selection and combination

1. Yinhua Zhang
2. Nathan A. Tanner

Reviewed by ScreenIT

Comparable neutralization of SARS-CoV-2 Delta AY.1 and Delta with individuals sera vaccinated with BBV152

1. Pragya D Yadav
2. Rima R Sahay
3. Gajanan Sapkal
4. Dimpal Nyayanit
5. Anita M Shete
6. Gururaj Deshpande
7. Deepak Y Patil
8. Nivedita Gupta
9. Sanjay Kumar
10. Priya Abraham
11. Samiran Panda
12. Balram Bhargava

Reviewed by ScreenIT

SARS-CoV-2 Fears Green: The Chlorophyll Catabolite Pheophorbide A Is a Potent Antiviral

1. Guillermo H. Jimenez-Aleman
2. Victoria Castro
3. Addis Londaitsbehere
4. Marta Gutierrez-Rodríguez
5. Urtzi Garaigorta
6. Roberto Solano
7. Pablo Gastaminza

Reviewed by ScreenIT

Defining Proximity Proteome of Histone Modifications by Antibody-Mediated Protein A-APEX2 Labeling

1. Xinran Li
2. Jiaqi Zhou
3. Wenjuan Zhao
4. Qing Wen
5. Weijie Wang
6. Huipai Peng
7. Yuan Gao
8. Kelly J. Bouchonville
9. Steven M. Offer
10. Kuiming Chan
11. Zhiquan Wang
12. Nan Li
13. Haiyun Gan

Reviewed by Review Commons

SARS-CoV-2 Restructures the Host Chromatin Architecture

1. Ruoyu Wang
2. Joo-Hyung Lee
3. Feng Xiong
4. Jieun Kim
5. Lana Al Hasani
6. Xiaoyi Yuan
7. Pooja Shivshankar
8. Joanna Krakowiak
9. Chuangye Qi
10. Yanyu Wang
11. Holger K. Eltzschig
12. Wenbo Li

Reviewed by ScreenIT

In situ imaging of bacterial outer membrane projections and associated protein complexes using electron cryo-tomography

1. Mohammed Kaplan
2. Georges Chreifi
3. Lauren Ann Metskas
4. Janine Liedtke
5. Cecily R Wood
6. Catherine M Oikonomou
7. William J Nicolas
8. Poorna Subramanian
9. Lori A Zacharoff
10. Yuhang Wang
11. Yi-Wei Chang
12. Morgan Beeby
13. Megan J Dobro
14. Yongtao Zhu
15. Mark J McBride
16. Ariane Briegel
17. Carrie L Shaffer
18. Grant J Jensen

Reviewed by Review Commons

Role of BRCA2 DNA-binding and C-terminal domain in its mobility and conformation in DNA repair

1. Maarten W Paul
2. Arshdeep Sidhu
3. Yongxin Liang
4. Sarah E van Rossum-Fikkert
5. Hanny Odijk
6. Alex N Zelensky
7. Roland Kanaar
8. Claire Wyman

• Curated by eLife

  Evaluation Summary:

  This work is of interest to readers in the field of genome stability, DNA repair and associated human diseases. The manuscript describes systematic analyses of the crucial DNA repair mediator BRCA2 and its variants lacking the DNA binding domain or RAD51 interacting C-terminal domain, and the conclusions present a conceptual advance as to how BRCA2 promotes DNA repair. The work is a technical tour de force that includes evaluation of the DNA damage response, gene targeting and single particle tracking in mouse embryonic stem cells, as well as biophysical analyses of the human counterparts. The key claims of the manuscript are largely supported by the data.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their names with the authors.)

Reviewed by eLife