Molecular Biology

Insights from aquaporin structures into drug-resistant sleeping sickness

1. Modestas Matusevicius
2. Robin A Corey
3. Marcos Gragera
4. Keitaro Yamashita
5. Teresa Sprenger
6. Marzuq Ungogo
7. James N Blaza
8. Pablo Castro-Hartmann
9. Dimitri Y Chirgadze
10. Sundeep Chaitanya Vedithi
11. Pavel Afanasyev
12. Roberto Melero
13. Rangana Warshamanage
14. Anastasiia Gusach
15. José-Maria Carazo
16. Mark Carrington
17. Tom Blundell
18. Garib N Murshudov
19. Phillip J Stansfeld
20. Mark SP Sansom
21. Harry P De Koning
22. Christopher G Tate
23. Simone Weyand

• Curated by eLife

  eLife Assessment

  In this important study, the authors set out to determine the molecular interactions between the AQP2 from Trypanosoma brucei (TbAQP2) and the trypanocidal drugs pentamidine and melarsoprol to understand how TbAQP2 mutations lead to drug resistance. Using cryo-EM, molecular dynamics simulations, and lysis assays the authors present convincing evidence that mutations in TbAQP2 make permeation of trypanocidal drugs energetically less favourable, and that this impacts the ability of drugs to achieve a therapeutic dose. Overall, this data will be of interest for those working on aquaporins, and development of trypanosomiasis drugs as well as drugs targeting aquaporins in general.

Reviewed by eLife

Cryo-EM Reveals Regulatory Mechanisms Governing Substrate Selection and Activation of Human LONP1

1. Jeffrey T Mindrebo
2. Lauren Alexandrescu
3. Jennifer R Baker
4. Garret Wang
5. Gabriel C Lander

• Curated by eLife

  eLife Assessment

  This valuable study presents EM structures of new conformational states of the LONP1 AAA+ protease in conjunction with the mitochondrial protein substrates (StAR, TFAM), along with biochemical functional assays. The EM structures revealed new conformational states in a closed configuration. The structures and associated functional results are solid. However, a notable weakness is the absence of substrates found threaded through the ATPase pores.

Reviewed by eLife

On the path to reference genomes for all biodiversity: lessons learned and laboratory protocols created in the Sanger Tree of Life core laboratory over the first 2000 species

1. Caroline Howard
2. Amy Denton
3. Benjamin Jackson
4. Adam Bates
5. Jessie Jay
6. Halyna Yatsenko
7. Priyanka Sethu Raman
8. Abitha Thomas
9. Graeme Oatley
10. Raquel Vionette do Amaral
11. Zeynep Ene Göktan
12. Juan Pablo Narváez Gómez
13. Isabelle Clayton Lucey
14. Elizabeth Sinclair
15. Michael A. Quail
16. Mark Blaxter
17. Kerstin Howe
18. Mara K. N. Lawniczak

Reviewed by GigaScience

CryoDataBot: a pipeline to curate cryoEM datasets for AI-driven structural biology

1. Qibo Xu
2. Leon Wu
3. Michael Rebelo
4. Shi Feng
5. Xinye Yu
6. Farhanaz Farheen
7. Daisuke Kihara
8. Z. Hong Zhou

Reviewed by GigaScience

Endogenous Real Time Imaging Reveals Dynamic Chromosomal Mobility During Ligand-Mediated Transcriptional Burst Events

1. Susan Wang
2. Thomas Suter
3. Amir Gamliel
4. Yeeun Kim
5. Sreejith J Nair
6. Soohwan Oh
7. Feng Yang
8. Kenneth A Ohgi
9. Tobias Wagner
10. Steven Gan
11. Michael G Rosenfeld

• Curated by eLife

  eLife Assessment

  This manuscript presents significant and important work that advances single-molecule imaging technology of transcription with simultaneous analysis of several parameters. However, currently, the evidence is incomplete and requires further quantitation/description of the technologies used, further controls, and additional analysis of the data by other methods.

Reviewed by eLife

Fibro-Adipogenic Progenitors require autocrine IGF-I in homeostatic and regenerating skeletal muscle

1. Yangyi E. Luo
2. Zoe Abe-Teh
3. Tarek Y. Alsaghir
4. Li-Ying Kuo
5. Fahong Yu
6. Bradley E. Stoker
7. Ambili Bai Appu
8. Yumei Zhou
9. Feng Yue
10. Daniel Kopinke
11. Elisabeth R. Barton

Reviewed by preLights

Bora Bridges Aurora-A Activation and Substrate Recognition of PLK1

1. Jennifer A Miles
2. Matthew Batchelor
3. Martin Walko
4. Vanda Gunning
5. Andrew J Wilson
6. Megan H Wright
7. Richard Bayliss

Reviewed by Review Commons

Cohesin reconstitution and homologous recombination repair of DNA double-strand breaks in late mitosis

1. Jessel Ayra Plasencia
2. Sara Medina-Suárez
3. Esperanza Hernández-Carralero
4. Jonay García-Luis
5. Lorraine S Symington
6. Félix Machín

• Curated by eLife

  eLife Assessment

  This study provides convincing evidence that homologous recombination can occur in telophase-arrested cells, independently of cohesin subunits Smc 1-3. These findings are valuable as they point to investigate the role of cohesins re-association with chromatin in the allelic inter-sister repair by homologous recombination.

Reviewed by eLife

TRF2 Non-Telomeric Function is Indispensable for Neural stemness

1. Soujanya Vinayagamurthy
2. Amit Kumar Bhatt
3. Sulochana Bagri
4. Supratim Ghosh
5. Arpan Parichha
6. Arindam Maitra
7. Shantanu Chowdhury

Reviewed by Review Commons

Epigenetic delineation of the earliest cardiac lineage segregation by single-cell multi-omics

1. Peng Xie
2. Xu Jiang
3. Jingjing He
4. Qingyun Pan
5. Xianfa Yang
6. Yanying Zheng
7. Zhuanzhuan Che
8. Wenli Fan
9. Chen Wu
10. Weiheng Zheng
11. Shuhan Si
12. Kun Gao
13. Shiqi Zhu
14. Ke Fang
15. Haitong Fang
16. Yi Yang
17. Tao P Zhong
18. Zhongzhou Yang
19. Ke Wei
20. Wei Xie
21. Naihe Jing
22. Zhuojuan Luo
23. Chengqi Lin

• Curated by eLife

  eLife Assessment

  This study provides new single-cell multi-omics datasets that may be useful in the study of early cardiac lineages. However, the authors' conclusions regarding the mutual regulation of key regulators for cardiac specification and new cardiac lineage trajectories are inadequately supported by persuasive analysis and do not align with prior published studies. If revised to address the serious caveats adequately, the findings may be of interest to researchers in the field of cardiac development and congenital heart disease.

Reviewed by eLife