Molecular Biology

Oxidized low-density lipoprotein potentiates angiotensin II-induced Gq activation through the AT1-LOX1 receptor complex: Implications for renal dysfunction

1. Jittoku Ihara
2. Yibin Huang
3. Yoichi Takami
4. Yoichi Nozato
5. Toshimasa Takahashi
6. Akemi Kakino
7. Cheng Wang
8. Ziwei Wang
9. Yu Guo
10. Weidong Liu
11. Nanxiang Yin
12. Ryoichi Ohara
13. Taku Fujimoto
14. Shino Yoshida
15. Kazuhiro Hongyo
16. Hiroshi Koriyama
17. Hiroshi Akasaka
18. Hikari Takeshita
19. Shinsuke Sakai
20. Kazunori Inoue
21. Yoshitaka Isaka
22. Hiromi Rakugi
23. Tatsuya Sawamura
24. Koichi Yamamoto

• Curated by eLife

  eLife assessment

  This study provides useful in vitro evidence to support a mechanism whereby dyslipidemia could accelerate renal functional decline through the activation of the AT1R/LOX1 complex by oxLDL and AngII. As such, it improves the knowledge regarding the complex interplay between dyslipidemia and renal disease and provides a solid basis for the discovery of novel therapeutic strategies for patients with lipid disorders. The methods, data, and analyses support the presented findings, although the observed variability and need for further in vivo validation require additional research in this key area.

Reviewed by eLife

Rtf1 HMD domain facilitates global histone H2B monoubiquitination and regulates morphogenesis and virulence in the meningitis-causing pathogen Cryptococcus neoformans

1. Yixuan Jiang
2. Fujie Zhao
3. Ying Liang
4. Zhenguo Lu
5. Siyu Wang
6. Yao Meng
7. Zhanxiang Liu
8. Jing Zhang
9. Youbao Zhao

• Curated by eLife

  eLife assessment

  This is an important study that connects the polymerase-associated factor 1 complex (Paf1C) with Histone 2B monoubiquitination and the expression of genes key to virulence in Cryptococcus neoformans. The provided information is convincing and has the potential to open several opportunities to further understand the basic biology of this significant human fungal pathogen.

Reviewed by eLife

Brown Adipose Tissue and Skeletal Muscle Coordinately Contribute to Thermogenesis in Mice

1. Yuna Izumi-Mishima
2. Rie Tsutsumi
3. Tetsuya Shiuchi
4. Saori Fujimoto
5. Momoka Taniguchi
6. Yuko Okamatsu-Ogura
7. Takeshi Yoneshiro
8. Masashi Kuroda
9. Kazuhiro Nomura
10. Hiroshi Sakaue

• Curated by eLife

  eLife assessment

  This is a useful paper regarding the roles of brown adipose tissue and skeletal muscle in thermogenesis in mice, with potential significance for the field. The overall approach is innovative but on balance the evidence for the claim is incomplete, as cast immobilization, while innovative, is likely stressful, may impact muscle and BAT directly, and imposes an energetic cost of motion on the animal that is not accounted for. Further experiments are also needed to directly assess the role of adipose-derived BCAAs in thermogenesis.

Reviewed by eLife

Inhibition of mitochondrial protein import and proteostasis by a pro-apoptotic lipid

1. Josep Fita-Torró
2. José Luis Garrido-Huarte
3. Agnès H Michel
4. Benoît Kornmann
5. Amparo Pascual-Ahuir
6. Markus Proft

• Curated by eLife

  eLife assessment

  This study is a valuable observation that deals with the toxic effects of an intermediary in lipid degradation [trans-2-hexadecenal (t-2-hex)] in yeast through modification of mitochondrial protein import via the TOM complex. However, we find that the claim that the TOM complex is a main target of t-2-hex are supported by incomplete evidence, thus allowing multiple various interpretation. Despite the shortcomings, this study is inspiring for researchers from the organellar, protein trafficking and lipid field and serves as a starting point to further precise and mechanistic analyses of the phenomenon.

Reviewed by eLife

An intestinal Sir2-HSF1-ATGL1 pathway regulates lipolysis in C. elegans

1. Milán Somogyvári
2. Saba Khatatneh
3. Gábor Hajdú
4. Bar Sotil
5. József Murányi
6. Csaba Sőti

Reviewed by Review Commons

An in vitro approach reveals molecular mechanisms underlying endocrine disruptor-induced epimutagenesis

1. Jake D Lehle
2. Yu-Huey Lin
3. Amanda Gomez
4. Laura Chavez
5. John R McCarrey

• Curated by eLife

  eLife assessment

  This important study, characterizing the epigenetic and transcriptomic response of a variety of cell types representative of somatic, germline, and pluripotent cells to BPS, reveals the cell type-specific changes in DNA methylation and the relationship with the genome sequence. The findings are convincing and provide a basis for future analyses in vivo. This work should be of interest to biomedical researchers who work on epigenetic reprogramming and epigenetic inheritance.

Reviewed by eLife

Epigenetic delineation of the earliest cardiac lineage segregation by single-cell multi-omics

1. Peng Xie
2. Xu Jiang
3. Jingjing He
4. Qingyun Pan
5. Xianfa Yang
6. Yanying Zheng
7. Wenli Fan
8. Chen Wu
9. Weiheng Zheng
10. Ke Fang
11. Shuhan Si
12. Shiqi Zhu
13. Yi Yang
14. Tao P Zhong
15. Zhongzhou Yang
16. Ke Wei
17. Wei Xie
18. Naihe Jing
19. Zhuojuan Luo
20. Chengqi Lin

• Curated by eLife

  eLife assessment

  This study combines extensive published and new datasets to provide a useful single-cell multi-omics analysis of early cardiac lineage segregation, highlighting the mutual regulation of key regulators for cardiac specification. While the data presentation is robust, the computational methods for delineating cardiac lineage trajectories and the functional analyses are incomplete and require further clarification and additional experiments. If validated, these findings will be of significant interest to researchers in the fields of cardiac development and congenital heart disease.

Reviewed by eLife

A fine kinetic balance of interactions directs transcription factor hubs to genes

1. Samantha Fallacaro
2. Apratim Mukherjee
3. Puttachai Ratchasanmuang
4. Joseph Zinski
5. Yara I Haloush
6. Kareena Shankta
7. Mustafa Mir

Reviewed by preLights

ACE2-independent sarbecovirus cell entry is supported by TMPRSS2-related enzymes and reduces sensitivity to antibody-mediated neutralization

1. Lu Zhang
2. Hsiu-Hsin Cheng
3. Nadine Krüger
4. Bojan Hörnich
5. Luise Graichen
6. Alexander S. Hahn
7. Sebastian R. Schulz
8. Hans-Martin Jäck
9. Metodi V. Stankov
10. Georg M.N. Behrens
11. Marcel A. Müller
12. Christian Drosten
13. Onnen Mörer
14. Martin Sebastian Winkler
15. ZhaoHui Qian
16. Stefan Pöhlmann
17. Markus Hoffmann

Reviewed by Review Commons

Multi-protein chimeric antigens, a novel combined approach for efficiently targeting and blocking the blood stage of Plasmodium falciparum

1. Bhagyashree Deshmukh
2. Dhruv Khatri
3. Sanjay Kumar Kochar
4. Chaitanya Athale
5. Krishanpal Karmodiya

Reviewed by Review Commons