Molecular Biology

NF-κB is a Central Regulator of Hypoxia-Induced Gene Expression

1. Dilem Shakir
2. Michael Batie
3. Chun-Sui Kwok
4. Simon J. Cook
5. Niall S. Kenneth
6. Sonia Rocha

Reviewed by Review Commons

RNase III cleavage signals spread across splice junctions enforce sequential processing of co-hosted snoRNAs

1. Valérie Migeot
2. Yves Mary
3. Etienne Fafard-Couture
4. Pierre Lombard
5. François Bachand
6. Michelle S Scott
7. Carlo Yague-Sanz

Reviewed by Review Commons

Negative regulation of miRNA sorting into EVs is mediated by the capacity of RBP PCBP2 to impair the SYNCRIP-dependent miRNA loading

1. Francesco Marocco
2. Sabrina Garbo
3. Claudia Montaldo
4. Alessio Colantoni
5. Luca Quattrocchi
6. Gioele Gaboardi
7. Giovanna Sabarese
8. Carla Cicchini
9. Mario Lecce
10. Alessia Carnevale
11. Rossella Paolini
12. Gian Gaetano Tartaglia
13. Cecilia Battistelli
14. Marco Tripodi

• Curated by eLife

  eLife Assessment

  This manuscript makes a valuable contribution to the field by uncovering a molecular mechanism for miRNA intracellular retention, mediated by the interaction of PCBP2, SYNCRIP, and specific miRNA motifs. The findings are convincing and advance our understanding of RNA-binding protein-mediated miRNA sorting, providing deeper insights into miRNA dynamics.

Reviewed by eLife

Structure of an oxygen-induced tubular nanocompartment in Pyrococcus furiosus

1. Wenfei Song
2. Jan Fiala
3. Ioannis Skalidis
4. Pascal Albanese
5. Constantinos Patinios
6. Marten L Chaillet
7. Servé WM Kengen
8. Richard A Scheltema
9. Stuart C Howes
10. Albert JR Heck
11. Friedrich Förster

• Curated by eLife

  eLife Assessment

  Using advanced CryoEM and mass spectrometry, the authors provide compelling evidence of how tubule formation occurs in an oxygen-dependent manner. These fundamental findings offer a novel mechanism by which rubrerythrin tubules encapsulate encapsulin to prevent oxidative stress in Pyrococcus furiosus. However, there are a few reasonable concerns about biochemical validations and the lack of adequate description of results and methodology.

Reviewed by eLife

Synonymous mutations in AAV Rep enhance genome packaging in a library selection

1. Tasfia Azim
2. Dru Myerscough
3. Jonathan J Silberg

• Curated by eLife

  eLife Assessment

  Engineering of adeno-associated virus (AAV) replication proteins may provide new insights into Parvoviral replication. The authors created a useful collection of Rep protein variants with changes that alter the amino acid sequence, but these did not lead to clear improvements in how the virus worked. Instead, their screen showed that changes that do not alter the protein ("synonymous" mutations) and changes to the promoter were more common. As it stands the results are incomplete due to potential issues with the screening design. We encourage a more complete characterization, which may enhance the translational potential of the approach.

Reviewed by eLife

PI3Kα inhibition blocks osteochondroprogenitor specification and the hyper-inflammatory response to prevent heterotopic ossification

1. José Antonio Valer
2. Alexandre Deber
3. Marius Wits
4. Carolina Pimenta-Lope
5. Marie-José Goumans
6. Jose Luis Rosa
7. Gonzalo Sánchez-Duffhues
8. Francesc Ventura

• Curated by eLife

  eLife Assessment

  This study, which includes additional experiments in response to the reviewer comments, presents valuable findings illustrating the role of PI3Kα in heterotopic ossification in FOP model mice. The methods, data, and analyses are solid and generally support the claims although as noted by one of the reviewers, there is no data demonstrating the effect of BYL79 on cell growth, and it remains unclear whether BYL79 also inhibits the Smad2/3 pathway. While this study provides new insights into the role of the PI3Kα pathway as a therapeutic target for FOP, questions about the mechanism of BYL79 still exist.

Reviewed by eLife

The Viral AlphaFold Database of monomers and homodimers reveals conserved protein folds in viruses of bacteria, archaea, and eukaryotes

1. Roni Odai
2. Michèle Leemann
3. Tamim Al-Murad
4. Minhal Abdullah
5. Lena Shyrokova
6. Tanel Tenson
7. Vasili Hauryliuk
8. Janani Durairaj
9. Joana Pereira
10. Gemma C. Atkinson

Reviewed by Arcadia Science

tRNA synthetase activity is required for stress granule and P-body assembly

1. Max Baymiller
2. Noah S. Helton
3. Benjamin Dodd
4. Stephanie L. Moon

Reviewed by preLights, Life Science Editors Foundation

Metabolic reprogramming by endothelial ANGPTL4 depletion protects against diabetic kidney disease

1. Swayam Prakash Srivastava
2. Han Zhou
3. Rachel Shenoi
4. Myshal Morris
5. Begoña Lainez-Mas
6. Yuta Takagaki
7. Barani Kumar Rajendran
8. Ocean Setia
9. Binod Aryal
10. Keizo Kanasaki
11. Daisuke Koya
12. Ken Inoki
13. Alan Dardik
14. Carlos Fernández-Hernando
15. Gerald I. Shulman
16. Julie E. Goodwin

Reviewed by Life Science Editors Foundation

Venomous Maggots? A first exploration of the toxin arsenal of larval stages of the horse fly Tabanus autumnalis

1. Jonas Krämer
2. Ludwig Dersch
3. Andreas Vilcinskas
4. Tim Lüddecke

Reviewed by Arcadia Science