Molecular Biology

TDP-43 directly inhibits RNA accumulation in neurites through modulation of RNA stability

1. Charlie Moffatt
2. Ankita Arora
3. Katherine F. Vaeth
4. Bryan B. Guzman
5. Gurprit Bhardwaj
6. Audrey Hoelscher
7. Levi B. Gifford
8. Holger A. Russ
9. Daniel Dominguez
10. J. Matthew Taliaferro

Reviewed by preLights

Sld3CBD–Cdc45 structural insights into Cdc45 recruitment for CMG complex formation on DNA replication

1. Hao Li
2. Izumi Ishizaki
3. Koji Kato
4. XiaoMei Sun
5. Sachiko Muramatsu
6. Hiroshi Itou
7. Toyoyuki Ose
8. Hiroyuki Araki
9. Min Yao

• Curated by eLife

  eLife Assessment

  This valuable paper describes the crystal structure of a complex of Sld3-Cdc45-binding domain (CBD) with Cdc45, which is essential for the assembly of an active Cdc45-MCM-GINS (CMG) double hexamers at the replication origin. Although the results shown in the paper are of interest to researchers in DNA replication and genome stability, the biochemical analysis of protein-protein interaction and DNA binding is incomplete, and the paper needs additional data.

Reviewed by eLife

Context specific ubiquitin modification of ribosomes regulates translation under oxidative stress

1. Shannon E. Dougherty
2. Géssica C. Barros
3. Matthew W. Foster
4. Guoshou Teo
5. Hyungwon Choi
6. Gustavo M. Silva

Reviewed by Review Commons

SFSWAP is a negative regulator of OGT intron detention and global pre-mRNA splicing

1. Ashwin Govindan
2. Nicholas K Conrad

• Curated by eLife

  eLife Assessment

  This study provides fundamental insights into the regulation of a retained intron in the mRNA coding for OGT, a process known to be regulated by the O-GlcNAc cycling system, and highlights the functional role of the splicing regulator SFSWAP. The evidence supporting the claims of the authors is convincing; the authors performed an elegant state-of-the-art CRISPR knockout strategy and sophisticated bioinformatic analysis to identify SFSWAP as a negative regulator of alternative splicing. The work will be of interest to researchers in the fields of splicing and glycobiology.

Reviewed by eLife

Inhibition of mitochondrial protein import and proteostasis by a pro-apoptotic lipid

1. Josep Fita-Torró
2. José Luis Garrido-Huarte
3. Lucía López-Gil
4. Agnès H Michel
5. Benoit Kornmann
6. Amparo Pascual-Ahuir
7. Markus Proft

• Curated by eLife

  eLife Assessment

  This study is a valuable observation that deals with the toxic effects of an intermediary in lipid degradation [trans-2-hexadecenal (t-2-hex)] in yeast through modification of mitochondrial protein import via the TOM complex. We find that the claim that the TOM complex is a main target of t-2-hex are supported by solid evidence, however the molecular mechanism remains unclear allowing multiple interpretation. Despite the shortcomings, this study is inspiring for researchers from the organellar, protein trafficking and lipid field and serves as a starting point to further precise and mechanistic analyses of the phenomenon.

Reviewed by eLife

Nitrosation of CD36 Regulates Endothelial Function and Serum Lipids

1. Melissa A. Luse
2. Wyatt J. Schug
3. Luke S. Dunaway
4. Shruthi Nyshadham
5. Skylar A. Loeb
6. Alicia Carvalho
7. Rachel Tessema
8. Caitlin Pavelec
9. T.C. Stevenson Keller
10. Xiaohong Shu
11. Claire A. Ruddiman
12. Anna Kosmach
13. Timothy M. Sveeggen
14. Ray Mitchell
15. Pooneh Bagher
16. Richard D. Minshall
17. Norbert Leitnger
18. Linda Columbus
19. Kandice R. Levental
20. Ilya Levental
21. Miriam Cortese-Krott
22. Brant E. Isakson

Reviewed by Life Science Editors Foundation

SIRT6 activator fucoidan extends healthspan and lifespan in aged wild-type mice

1. Seyed A. Biashad
2. Eric Hillpot
3. Francesco Morandini
4. Cheyenne Rechsteiner
5. Victoria Paige
6. Gregory Tombline
7. Minseon Lee
8. Zhizhong Zheng
9. Yuan Liang
10. John Martinez
11. Natasha Sieczkiewicz
12. Zhihui Zhang
13. Valentin Volobaev
14. Denis Firsanov
15. Matthew Simon
16. Lei J. Zhang
17. Paul D. Robbins
18. Andrei Seluanov
19. Vera Gorbunova

Reviewed by Life Science Editors Foundation

Clu1/Clu form mitochondria-associated granules upon metabolic transitions and regulate mitochondrial protein translation via ribosome interactions

1. Leonor Miller-Fleming
2. Wing Hei Au
3. Laura Raik
4. Pedro Guiomar
5. Jasper Schmitz
6. Ha Yoon Cho
7. Aron Czako
8. Alexander J Whitworth

Reviewed by Review Commons

In silico screening by AlphaFold2 program revealed the potential binding partners of nuage-localizing proteins and piRNA-related proteins

1. Shinichi Kawaguchi
2. Xin Xu
3. Takashi Soga
4. Kenta Yamaguchi
5. Ryuuya Kawasaki
6. Ryota Shimouchi
7. Susumu Date
8. Toshie Kai

• Curated by eLife

  eLife Assessment

  This useful study employs AlphaFold2 to predict interactions among 20 nuage proteins, identifying five novel interaction candidates, three of which are validated experimentally through co-immunoprecipitation. Expanding the analysis to 430 oogenesis-related proteins and screening ~12,000 Drosophila proteins for interactions with Piwi, the study identifies 164 potential binding partners, demonstrating how computational predictions can streamline experimental validation. This study provides a solid basis for further investigations into eukaryotic protein interaction networks.

Reviewed by eLife

Optogenetic control of Protein Kinase C-epsilon activity reveals its intrinsic signaling properties with spatiotemporal resolution

1. Qunxiang Ong
2. Crystal Jing Yi Lim
3. Yilie Liao
4. Justin Tze-Yang Ng
5. Ler Ting Rachel Lim
6. Shernys Xuan Yi Koh
7. Sher En Chan
8. Pheobe Lee Yu Ying
9. Huijun Lim
10. Chen Rui Ye
11. Loo Chien Wang
12. Siok Ghee Ler
13. Radoslaw M Sobota
14. Yaw Sing Tan
15. Gerald I Shulman
16. Xiaoyong Yang
17. Weiping Han

• Curated by eLife

  eLife Assessment

  This manuscript introduces Opto-PKCε, an optogenetic tool that enabled important findings derived from interactome and phosphoproteome studies. Light-dependent recruitment of Opto-PKCε to the plasma membrane revealed the specific phosphorylation of the insulin receptor at Thr 1160. In turn, recruitment to mitochondria led to phosphorylation of the complex I subunit NDUFS4, correlating with reduced spare respiratory capacity. The evidence supporting these conclusions is solid, although additional clarification on data analysis would further enhance readability.

Reviewed by eLife