Molecular Biology

NASP functions in the cytoplasm to prevent histone H3 aggregation during early embryogenesis

1. Mohit Das
2. Eli Coronado-Chavez
3. Anusha D Bhatt
4. Reyhaneh Tirgar
5. Amanda A Amodeo
6. Jared Nordman

Reviewed by Review Commons

Inhibiting Glutamine Metabolism Blocks Coronavirus Replication in Mammalian Cells

1. Kai Su Greene
2. Annette Choi
3. Matthew Chen
4. Nianhui Yang
5. Ruizhi Li
6. Yijian Qiu
7. Michael J Lukey
8. Katherine S Rojas
9. Jonathan Shen
10. Kristin F Wilson
11. William P Katt
12. Gary R Whittaker
13. Richard A Cerione

Reviewed by Rapid Reviews Infectious Diseases

Malaria parasite resistance to azithromycin is not readily transmitted by mosquitoes

1. Hayley D. Buchanan
2. Robyn McConville
3. Lee M. Yeoh
4. Michael F. Duffy
5. Justin A. Boddey
6. Geoffrey I. McFadden
7. Christopher D. Goodman

• Curated by eLife

  eLife assessment

  This important work addressed the identifcation of antimalarial drug resistance mutations that do not readily transmit to new human hosts, focusing on azithromycin resistance. The technically challenging analyses of azithromycin-resistant parasites as they traverse the mosquito host and human liver are conducted using state-of-the-art tools, including humanized mice. While the claim regarding the lack of transmission by atovaquone-resistant P. berghei is convincing, the evidence for the lack of transmission by atovaquone-resistant P. falciparum is insufficient. This work will appeal to biologists and biomedical scientists in parasitology and drug discovery, offering insights into combating antimalarial drug resistance and understanding the fitness costs associated with drug-resistant parasites.

Reviewed by eLife, Rapid Reviews Infectious Diseases

Single cell transcriptomics and development of gametocyte-specific molecular markers for avian malaria parasites

1. A Berthomieu
2. S Gandon
3. AM Talman
4. A Rivero

Reviewed by PREreview

Insights from aquaporin structures into drug-resistant sleeping sickness

1. Modestas Matusevicius
2. Robin A Corey
3. Marcos Gragera
4. Keitaro Yamashita
5. Teresa Sprenger
6. Marzuq Ungogo
7. James N Blaza
8. Pablo Castro-Hartmann
9. Dimitri Y Chirgadze
10. Sundeep Chaitanya Vedithi
11. Pavel Afanasyev
12. Roberto Melero
13. Rangana Warshamanage
14. Anastasiia Gusach
15. José-Maria Carazo
16. Mark Carrington
17. Tom Blundell
18. Garib N Murshudov
19. Phillip J Stansfeld
20. Mark SP Sansom
21. Harry P De Koning
22. Christopher G Tate
23. Simone Weyand

• Curated by eLife

  eLife Assessment

  In this important study, the authors set out to determine the molecular interactions between the AQP2 from Trypanosoma brucei (TbAQP2) and the trypanocidal drugs pentamidine and melarsoprol to understand how TbAQP2 mutations lead to drug resistance. Using cryo-EM, molecular dynamics simulations, and lysis assays the authors present convincing evidence that mutations in TbAQP2 make permeation of trypanocidal drugs energetically less favourable, and that this impacts the ability of drugs to achieve a therapeutic dose. Overall, this data will be of interest for those working on aquaporins, and development of trypanosomiasis drugs as well as drugs targeting aquaporins in general.

Reviewed by eLife

Cryo-EM Reveals Regulatory Mechanisms Governing Substrate Selection and Activation of Human LONP1

1. Jeffrey T Mindrebo
2. Lauren Alexandrescu
3. Jennifer R Baker
4. Garret Wang
5. Gabriel C Lander

• Curated by eLife

  eLife Assessment

  This valuable study presents EM structures of new conformational states of the LONP1 AAA+ protease in conjunction with the mitochondrial protein substrates (StAR, TFAM), along with biochemical functional assays. The EM structures revealed new conformational states in a closed configuration. The structures and associated functional results are solid. However, a notable weakness is the absence of substrates found threaded through the ATPase pores.

Reviewed by eLife

On the path to reference genomes for all biodiversity: lessons learned and laboratory protocols created in the Sanger Tree of Life core laboratory over the first 2000 species

1. Caroline Howard
2. Amy Denton
3. Benjamin Jackson
4. Adam Bates
5. Jessie Jay
6. Halyna Yatsenko
7. Priyanka Sethu Raman
8. Abitha Thomas
9. Graeme Oatley
10. Raquel Vionette do Amaral
11. Zeynep Ene Göktan
12. Juan Pablo Narváez Gómez
13. Isabelle Clayton Lucey
14. Elizabeth Sinclair
15. Michael A. Quail
16. Mark Blaxter
17. Kerstin Howe
18. Mara K. N. Lawniczak

Reviewed by GigaScience

CryoDataBot: a pipeline to curate cryoEM datasets for AI-driven structural biology

1. Qibo Xu
2. Leon Wu
3. Michael Rebelo
4. Shi Feng
5. Xinye Yu
6. Farhanaz Farheen
7. Daisuke Kihara
8. Z. Hong Zhou

Reviewed by GigaScience

Endogenous Real Time Imaging Reveals Dynamic Chromosomal Mobility During Ligand-Mediated Transcriptional Burst Events

1. Susan Wang
2. Thomas Suter
3. Amir Gamliel
4. Yeeun Kim
5. Sreejith J Nair
6. Soohwan Oh
7. Feng Yang
8. Kenneth A Ohgi
9. Tobias Wagner
10. Steven Gan
11. Michael G Rosenfeld

• Curated by eLife

  eLife Assessment

  This manuscript presents significant and important work that advances single-molecule imaging technology of transcription with simultaneous analysis of several parameters. However, currently, the evidence is incomplete and requires further quantitation/description of the technologies used, further controls, and additional analysis of the data by other methods.

Reviewed by eLife

Fibro-Adipogenic Progenitors require autocrine IGF-I in homeostatic and regenerating skeletal muscle

1. Yangyi E. Luo
2. Zoe Abe-Teh
3. Tarek Y. Alsaghir
4. Li-Ying Kuo
5. Fahong Yu
6. Bradley E. Stoker
7. Ambili Bai Appu
8. Yumei Zhou
9. Feng Yue
10. Daniel Kopinke
11. Elisabeth R. Barton

Reviewed by preLights