Molecular Biology

PDZ-directed substrate recruitment is the primary determinant of specific 4E-BP1 dephosphorylation by PP1-Neurabin

1. Roman O Fedoryshchak
2. Karim El-Bouri
3. Dhira Joshi
4. Stephane Mouilleron
5. Richard Treisman

• Curated by eLife

  eLife Assessment

  This important study reports on a basis for neurabin-mediated specification of substrate choice by protein phosphatase-1. The data from the comprehensive approach using structural, biochemical, and computational methods are compelling. This paper is broadly relevant to those investigating various cellular signaling cascades that entail phosphorylation as the main mechanism.

Reviewed by eLife

Structural basis of lipopolysaccharide translocon assembly mediated by the small lipoprotein LptM

1. Ryoji Miyazaki
2. Mai Kimoto
3. Hidetaka Kohga
4. Tomoya Tsukazaki

Reviewed by PREreview

Ribosomal RNA synthesis by RNA polymerase I is regulated by premature termination of transcription

1. Chaïma Azouzi
2. Katrin Schwank
3. Sophie Queille
4. Marta Kwapisz
5. Marion Aguirrebengoa
6. Anthony Henras
7. Simon Lebaron
8. Herbert Tschochner
9. Annick Lesne
10. Frédéric Beckouët
11. Olivier Gadal
12. Christophe Dez

• Curated by eLife

  eLife Assessment

  This manuscript characterizes a mutated clone of RNA polymerase I in yeast, referred to as SuperPol, to understand the mechanisms of RNA polymerase I elongation and termination. The authors present solid evidence that SuperPol has higher processivity during transcription elongation than wild-type RNA polymerase I. Notably, the study provides evidence that the transcriptional pause of RNA polymerase I may be a therapeutic vulnerability in cancers. Overall, the characterization of this RNA pol I is important as it provides insights into the regulation of ribosomal RNA transcription and its potential application in cancer pharmacology.

  [Editors' note: this paper was reviewed by Review Commons.]

Reviewed by eLife, Review Commons

A conserved mechanism for the retrieval of polyubiquitinated proteins from cilia

1. Sven M. Lange
2. Robyn J. Eisert
3. Alan Brown

Reviewed by Arcadia Science

Rapid Raman spectroscopy-based test for antimicrobial resistance

1. Vladimir Mushenkov
2. Ksenia Zhigalova
3. Pavel Denisov
4. Alexey Gordeev
5. Dmitry Lukyanov
6. Vladimir Kukushkin
7. Tatiana Priputnevich
8. Elena Zavyalova

Reviewed by Arcadia Science

Autophagy Suppresses CCL2 to Preserve Appetite and Prevent Lethal Cachexia

1. Maria Ibrahim
2. Maria Gomez-Jenkins
3. Adina Scheinfeld
4. Zhengqiao Zhao
5. Eduardo Cararo Lopes
6. Akshada Sawant
7. Zhixian Hu
8. Aditya Dharani
9. Michael Sun
10. Sarah Siddiqui
11. Emily T. Mirek
12. Johan Abram-Saliba
13. Edmund C. Lattime
14. Xiaoyang Su
15. Tobias Janowitz
16. Marcus D. Goncalves
17. Steven M. Dunn
18. Yuri Pritykin
19. Tracy G. Anthony
20. Joshua D. Rabinowitz
21. Eileen White

Reviewed by Life Science Editors Foundation

Rtf1 HMD domain facilitates global histone H2B monoubiquitination and regulates morphogenesis and virulence in the meningitis-causing pathogen Cryptococcus neoformans

1. Yixuan Jiang
2. Ying Liang
3. Fujie Zhao
4. Zhenguo Lu
5. Siyu Wang
6. Yao Meng
7. Zhanxiang Liu
8. Jing Zhang
9. Youbao Zhao

• Curated by eLife

  eLife Assessment

  This is an important study that connects the polymerase-associated factor 1 complex (Paf1C) with Histone 2B monoubiquitination and the expression of genes key to virulence in Cryptococcus neoformans. The provided information is convincing and has the potential to open several opportunities to further understand the basic biology of this significant human fungal pathogen.

Reviewed by eLife

TDP-43 directly inhibits RNA accumulation in neurites through modulation of RNA stability

1. Charlie Moffatt
2. Ankita Arora
3. Katherine F. Vaeth
4. Bryan B. Guzman
5. Gurprit Bhardwaj
6. Audrey Hoelscher
7. Levi B. Gifford
8. Holger A. Russ
9. Daniel Dominguez
10. J. Matthew Taliaferro

Reviewed by preLights

Context specific ubiquitin modification of ribosomes regulates translation under oxidative stress

1. Shannon E. Dougherty
2. Géssica C. Barros
3. Matthew W. Foster
4. Guoshou Teo
5. Hyungwon Choi
6. Gustavo M. Silva

Reviewed by Review Commons

SFSWAP is a negative regulator of OGT intron detention and global pre-mRNA splicing

1. Ashwin Govindan
2. Nicholas K Conrad

• Curated by eLife

  eLife Assessment

  This study provides fundamental insights into the regulation of a retained intron in the mRNA coding for OGT, a process known to be regulated by the O-GlcNAc cycling system, and highlights the functional role of the splicing regulator SFSWAP. The evidence supporting the claims of the authors is convincing; the authors performed an elegant state-of-the-art CRISPR knockout strategy and sophisticated bioinformatic analysis to identify SFSWAP as a negative regulator of alternative splicing. The work will be of interest to researchers in the fields of splicing and glycobiology.

Reviewed by eLife