Molecular Biology

A local ATR-dependent checkpoint pathway is activated by a site-specific replication fork block in human cells

1. Sana Ahmed-Seghir
2. Manisha Jalan
3. Helen E Grimsley
4. Aman Sharma
5. Shyam Twayana
6. Settapong T Kosiyatrakul
7. Christopher Thompson
8. Carl L Schildkraut
9. Simon N Powell

• Curated by eLife

  eLife assessment

  This manuscript reports important data on the cellular response to a single site-specific replication fork block in human MCF7 cells. Compelling evidence shows the efficacy of the bacterial Tus-Ter system to stall replication forks in human cells. Fork stalling let to lasting ATR-dependent phosphorylation of histone H2AX but not of ATR itself and its downstream targets RPA and CHK1.

Reviewed by eLife

Defining amino acid pairs as structural units suggests mutation sensitivity to adjacent residues

1. Aviv A. Rosenberg
2. Nitsan Yehishalom
3. Ailie Marx
4. Alex Bronstein

Reviewed by PREreview

lncRNA read-through regulates the BX-C insulator Fub-1

1. Airat Ibragimov
2. Xin Yang Bing
3. Yulii V Shidlovskii
4. Michael Levine
5. Pavel Georgiev
6. Paul Schedl

• Curated by eLife

  eLife assessment

  This fundamental work substantially advances our understanding of how neighboring genes on a chromosome can be separately controlled in time and space. The evidence supporting the conclusions is compelling, with state-of-the-art genetic perturbations and imaging. The work will be of broad interest to geneticists and cell biologists.

Reviewed by eLife

Inhibition of type I PRMTs reforms muscle stem cell identity enhancing their therapeutic capacity

1. Claudia Dominici
2. Oscar D Villarreal
3. Junio Dort
4. Emilie Heckel
5. Yu Chang Wang
6. Ioannis Ragoussis
7. Jean-Sebastien Joyal
8. Nicolas Dumont
9. Stéphane Richard

• Curated by eLife

  eLife assessment

  This valuable paper informs on the role of type I PRMTs in programming muscle stem cell identification. The evidence presented is mostly solid, with some weaknesses in the evidence regarding the proposed mechanism. The paper will be of particular interest to those who study skeletal muscle satellite cell biology.

Reviewed by eLife, Arcadia Science

CRISPR/Cas9 mediated gene editing in non-model nematode Panagrolaimus sp. PS1159

1. Viktoria Hellekes
2. Denise Claus
3. Johanna Seiler
4. Felix Illner
5. Philipp H. Schiffer
6. Michael Kroiher

Reviewed by Arcadia Science

A novel eukaryotic RdRP-dependent small RNA pathway represses antiviral immunity by controlling an ERK pathway component in the black-legged tick

1. Canran Feng
2. Kyosuke Torimaru
3. Mandy Yu Theng Lim
4. Li-Ling Chak
5. Masami Shiimori
6. Kosuke Tsuji
7. Tetsuya Tanaka
8. Junko Iida
9. Katsutomo Okamura

Reviewed by Arcadia Science

Iterative immunostaining combined with expansion microscopy and image processing reveals nanoscopic network organization of nuclear lamina

1. Elina Mäntylä
2. Toni Montonen
3. Lucio Azzari
4. Salla Mattola
5. Markus Hannula
6. Maija Vihinen-Ranta
7. Jari Hyttinen
8. Minnamari Vippola
9. Alessandro Foi
10. Soile Nymark
11. Teemu O. Ihalainen

Reviewed by Arcadia Science

Exploring G-quadruplex structure in PRCC-TFE3 fusion Oncogene: Plausible use as anti cancer therapy for translocation Renal cell carcinoma (tRCC)

1. Neha
2. Parimal Das

Reviewed by PREreview

Deficiency of the lipid flippase ATP10A causes diet-induced dyslipidemia in female mice

1. Adriana C. Norris
2. Eugenia M. Yazlovitskaya
3. Lin Zhu
4. Bailey S. Rose
5. Jody C. May
6. Katherine N. Gibson-Corley
7. John A. McLean
8. John M. Stafford
9. Todd R. Graham

Reviewed by PREreview

Molecular basis of interactions between CaMKII and α-actinin-2 that underlie dendritic spine enlargement

1. Ashton J Curtis
2. Jian Zhu
3. Christopher J Penny
4. Matthew G Gold

• Curated by eLife

  eLife assessment

  This fundamental study from Gold and colleagues substantially advances our understanding of the synaptic targeting of a major postsynaptic protein kinase, CaMKII, which is the basis for the persistence of excitatory synaptic strength in synaptic plasticity. The evidence supporting the claims of the authors is convincing, with cell biological, biochemical, as well as structural biological approaches. This work will be of interest to cell and computational biologists working on learning/memory.

Reviewed by eLife