Molecular Biology

An ultrasensitive, rapid, and portable coronavirus SARS-CoV-2 sequence detection method based on CRISPR-Cas12

1. Curti Lucia
2. Pereyra-Bonnet Federico
3. Gimenez Carla Alejandra

Reviewed by ScreenIT

Structural and Evolutionary Analysis Indicate that the SARS-CoV-2 Mpro is an Inconvenient Target for Small-Molecule Inhibitors Design

1. Maria Bzówka
2. Karolina Mitusińska
3. Agata Raczyńska
4. Aleksandra Samol
5. Jack A. Tuszyński
6. Artur Góra

Reviewed by ScreenIT

A simple magnetic nanoparticles-based viral RNA extraction method for efficient detection of SARS-CoV-2

1. Zhen Zhao
2. Haodong Cui
3. Wenxing Song
4. Xiaoling Ru
5. Wenhua Zhou
6. Xuefeng Yu

Reviewed by ScreenIT

Intrinsic protein disorder is insufficient to drive subnuclear clustering in embryonic transcription factors

1. Colleen E Hannon
2. Michael B Eisen

• Curated by eLife

  eLife assessment

  The manuscript addresses a fundamental question: are IDRs responsible for subnuclear clustering of transcription factors? A screen of 75 IDRs yielded convincing evidence that IDRs are rarely sufficient for subnuclear clustering, while the experimental design and data analysis provided limited evidence for the authors' claims regarding transcription factor clustering.

Reviewed by eLife

FAM111A regulates replication origin activation and cell fitness

1. Diana O. Rios-Szwed
2. Elisa Garcia-Wilson
3. Luis Sanchez-Pulido
4. Vanesa Alvarez
5. Hao Jiang
6. Susanne Bandau
7. Angus Lamond
8. Chris P. Ponting
9. Constance Alabert

Reviewed by Review Commons

Tunable DNMT1 degradation reveals cooperation of DNMT1 and DNMT3B in regulating DNA methylation dynamics and genome organization

1. Andrea Scelfo
2. Viviana Barra
3. Nezar Abdennur
4. George Spracklin
5. Florence Busato
6. Catalina Salinas-Luypaert
7. Elena Bonaiti
8. Guillaume Velasco
9. Anna Chipont
10. Coralie Guérin
11. Andréa E. Tijhuis
12. Diana C.J. Spierings
13. Claire Francastel
14. Floris Foijer
15. Jorg Tost
16. Leonid Mirny
17. D. Fachinetti

Reviewed by Review Commons, PREreview

High-precision mapping of nuclear pore-chromatin interactions reveals new principles of genome organization at the nuclear envelope

1. Swati Tyagi
2. Juliana S. Capitanio
3. Jiawei Xu
4. Fei Chen
5. Rahul Sharma
6. Jialiang Huang
7. Martin W. Hetzer

• Curated by eLife

  eLife assessment

  This manuscript describes important findings that will impact our understanding of nuclear pore complex (NPC)-genome interactions and how nuclear pore proteins (nucleoporins) impact super enhancer function. The authors develop a clever new approach termed NPC-DamID to map chromatin-NPC interactions that has several important advantages over existing techniques and may be used in contexts that are incompatible with genetic manipulation. The authors provide compelling evidence that nucleoporins interact with super enhancers in multiple cell types and also suggest that the phase separation behavior of nucleoporins contributes to the hierarchical organization of super enhancers, which is exciting and will stimulate additional work.

Reviewed by eLife

TCR-pMHC complex formation triggers CD3 dynamics

1. Floris J. van Eerden
2. Alrahman Aalaa Sherif
3. Mara Anais Llamas-Covarrubias
4. Arthur Millius
5. Xiuyuan Lu
6. Shigenari Ishizuka
7. Sho Yamasaki
8. Daron M. Standley

• Curated by eLife

  eLife assessment

  There was a range of opinion among three highly expert reviewers from different perspectives in the field. This is a significant topic and it was felt that the contribution at present is valuable to those in the field. However, it was agreed after consultation that the description of the simulation methodology was inadequate.

Reviewed by eLife

p38γ and p38δ modulate innate immune response by regulating MEF2D activation

1. Alejandra Escós
2. Ester Diaz-Mora
3. Michael Pattison
4. Pilar Fajardo
5. Diego González-Romero
6. Ana Risco
7. José Martín-Gómez
8. Éric Bonneil
9. Nahum Sonenberg
10. Seyed Mehdi Jafarnejad
11. Juan José Sanz-Ezquerro
12. Steven C Ley
13. Ana Cuenda

• Curated by eLife

  eLife assessment

  The authors have established a model for studying p38g/d signaling, which is an important accomplishment given that previous models have been compromised by changes in the TPL2/ERK pathway. Compelling evidence is presented to support the conclusions.

Reviewed by eLife

The obesity-linked human lncRNA AATBC regulates adipocyte plasticity by stimulating mitochondrial dynamics and respiration

1. Maude Giroud
2. Stefan Kotschi
3. Yun Kwon
4. Ophélia Le Thuc
5. Anne Hoffmann
6. Manuel Gil-Lozano
7. Michael Karbiener
8. Juan Carlos Higareda-Almaraz
9. Sajjad Khani
10. Daniel Tews
11. Pamela Fischer-Posovszky
12. Wenfei Sun
13. Hua Dong
14. Adhideb Ghosh
15. Christian Wolfrum
16. Martin Wabitsch
17. Kirsi A. Virtanen
18. Matthias Blüher
19. Søren Nielsen
20. Anja Zeigerer
21. Cristina García-Cáceres
22. Marcel Scheideler
23. Stephan Herzig
24. Alexander Bartelt

Reviewed by Review Commons