Molecular Biology

Scaling of an antibody validation procedure enables quantification of antibody performance in major research applications

1. Riham Ayoubi
2. Joel Ryan
3. Michael S Biddle
4. Walaa Alshafie
5. Maryam Fotouhi
6. Sara Gonzalez Bolivar
7. Vera Ruiz Moleon
8. Peter Eckmann
9. Donovan Worrall
10. Ian McDowell
11. Kathleen Southern
12. Wolfgang Reintsch
13. Thomas M Durcan
14. Claire Brown
15. Anita Bandrowski
16. Harvinder Virk
17. Aled M Edwards
18. Peter McPherson
19. Carl Laflamme

• Curated by eLife

  eLife assessment

  Antibodies are some of the most critical tools in biomedical research. However, their quality and specificity vary significantly. This fundamental study provides guidelines for how the quality of an antibody should be assessed and recorded and provides compelling data on the selected antibodies. This paper will be of interest to researchers working in experimental cell biology.

Reviewed by eLife

Rapid cloning-free mutagenesis of new SARS-CoV-2 variants using a novel reverse genetics platform

1. Enja Tatjana Kipfer
2. David Hauser
3. Martin J Lett
4. Fabian Otte
5. Lorena Urda
6. Yuepeng Zhang
7. Christopher MR Lang
8. Mohamed Chami
9. Christian Mittelholzer
10. Thomas Klimkait

• Curated by eLife

  eLife assessment

  This study describes CLEVER, an improved method for fast and efficient rescue and mutagenesis of SARS-CoV2. While the principle of this method is not new, this work significantly improves upon existing protocols, providing an important advancement in the field of viral infectious clones. Convincing proof-of-concept experiments were performed that demonstrate the utility and efficiency of the method.

Reviewed by eLife

The long non-coding RNA LINC00941 modulates MTA2/NuRD occupancy to suppress premature human epidermal differentiation

1. Eva Morgenstern
2. Uwe Schwartz
3. Johannes Graf
4. Astrid Bruckmann
5. Markus Kretz

Reviewed by Review Commons

TAX1BP1 recruits ATG9 vesicles through SCAMP3 binding

1. Yutaro Hama
2. Yoshitaka Kurikawa
3. Takahide Matsui
4. Noboru Mizushima
5. Hayashi Yamamoto

Reviewed by Review Commons

Multiple cullin-associated E3 ligases regulate cyclin D1 protein stability

1. Ke Lu
2. Ming Zhang
3. Guizheng Wei
4. Guozhi Xiao
5. Liping Tong
6. Di Chen

• Curated by eLife

  eLife assessment

  This paper is of interest to cell biologists studying the mechanisms of protein posttranslational modifications. The study investigates Cullin-RING ubiquitin E3 ligases (CRLs) that regulate cyclin D1 protein stability in cells by utilizing siRNA screening and ectopic expression approaches. By screening a siRNA library containing different E3 ligases, the authors identified a previously uncharacterized combination of Cullin 1-7 and associated E3 ligases (Keap1-CUL3, DDB2-CUL4A/4B, WSB2-CUL2/5, and RBX1-CUL1-7) that are important for cyclin D1 ubiquitination and proteasome-mediated degradation.

Reviewed by eLife

Tardigrades dramatically upregulate DNA repair pathway genes in response to ionizing radiation

1. Courtney M. Clark-Hachtel
2. Jonathan D. Hibshman
3. Tristan De Buysscher
4. Bob Goldstein

Reviewed by preLights

ZMYM2 controls human transposable element transcription through distinct co-regulatory complexes

1. Danielle J Owen
2. Elisa Aguilar-Martinez
3. Zongling Ji
4. Yaoyong Li
5. Andrew D Sharrocks

Reviewed by eLife

Transcription-independent hold of the G1/S transition is exploited to cope with DNA replication stress

1. Yue Jin
2. Guoqing Lan
3. Jiaxin Zhang
4. Haoyuan Sun
5. Li Xin
6. Qinhong Cao
7. Chao Tang
8. Xiaojing Yang
9. Huiqiang Lou
10. Wenya Hou

Reviewed by Review Commons

The helicase domain of human Dicer prevents RNAi-independent activation of antiviral and inflammatory pathways

1. Morgane Baldaccini
2. Léa Gaucherand
3. Béatrice Chane-Woon-Ming
4. Mélanie Messmer
5. Floriane Gucciardi
6. Sébastien Pfeffer

Reviewed by Review Commons

Multiplex DNA fluorescence in situ hybridization to analyze maternal vs. paternal C. elegans chromosomes

1. Silvia Gutnik
2. Ahilya Sawh
3. Susan E. Mango

Reviewed by Review Commons