Molecular Biology

CRISPR-dependent Base Editing Screens Identify Separation of Function Mutants of RADX with Altered RAD51 Regulatory Activity

1. Madison B. Adolph
2. Atharv S. Garje
3. Swati Balakrishnan
4. Florian Morati
5. Mauro Modesti
6. Walter J. Chazin
7. David Cortez

Reviewed by preLights

Systematic Analysis of Network-driven Adaptive Resistance to CDK4/6 and Estrogen Receptor Inhibition using Meta-Dynamic Network Modelling

1. Anthony Hart
2. Sung-Young Shin
3. Lan K. Nguyen

• Curated by eLife

  eLife assessment

  This manuscript presents a useful method for a comprehensive numerical simulation to systematically characterise the effect of heterogeneity in either the initial conditions or the biophysical parameters on the dynamic behaviour of protein signalling networks. Nevertheless, the presentation and detail of their model appear incomplete to fully support the main claims of the manuscript.

Reviewed by eLife

SIRT2 inhibition protects against cardiac hypertrophy and ischemic injury

1. Xiaoyan Yang
2. Hsiang-Chun Chang
3. Yuki Tatekoshi
4. Amir Mahmoodzadeh
5. Maryam Balibegloo
6. Zeinab Najafi
7. Rongxue Wu
8. Chunlei Chen
9. Tatsuya Sato
10. Jason Shapiro
11. Hossein Ardehali

• Curated by eLife

  eLife assessment

  In this study, Yang et al. have shown that SIRT2 has adverse effects on the heart in response to injury. Further, they demonstrate that deletion of Sirt2 is protective through stabilization and increased nuclear translocation of NRF2, which leads to increased expression of antioxidant genes. They also show that pharmacological inhibition of SIRT2 protects the heart against the development of cardiac hypertrophy.

Reviewed by eLife

N -6-methyladenosine (m6A) promotes the nuclear retention of mRNAs with intact 5′ splice site motifs

1. Eliza S Lee
2. Harrison W Smith
3. Yifan E Wang
4. Sean SJ Ihn
5. Leticia Scalize de Oliveira
6. Nevraj S Kejiou
7. Yijing L Liang
8. Syed Nabeel-Shah
9. Robert Y Jomphe
10. Shuye Pu
11. Jack F Greenblatt
12. Alexander F Palazzo

Reviewed by Review Commons

BATF relieves hepatic steatosis by inhibiting PD1 and promoting energy metabolism

1. Zhiwang Zhang
2. Qichao Liao
3. Tingli Pan
4. Lin Yu
5. Zupeng Luo
6. Songtao Su
7. Shi Liu
8. Menglong Hou
9. Yixing Li
10. Turtushikh Damba
11. Yunxiao Liang
12. Lei Zhou

• Curated by eLife

  eLife assessment

  This valuable study presents reports on the role of the transcription factor BATF and its target PD1 in lipid metabolism including a model of nonalcoholic fatty liver disease (NAFLD). Overall, the evidence supporting the conclusions is convincing. The work will be of interest to medical biologists working on NAFLD.

Reviewed by eLife

A local ATR-dependent checkpoint pathway is activated by a site-specific replication fork block in human cells

1. Sana Ahmed-Seghir
2. Manisha Jalan
3. Helen E Grimsley
4. Aman Sharma
5. Shyam Twayana
6. Settapong T Kosiyatrakul
7. Christopher Thompson
8. Carl L Schildkraut
9. Simon N Powell

• Curated by eLife

  eLife assessment

  This manuscript reports important data on the cellular response to a single site-specific replication fork block in human MCF7 cells. Compelling evidence shows the efficacy of the bacterial Tus-Ter system to stall replication forks in human cells. Fork stalling let to lasting ATR-dependent phosphorylation of histone H2AX but not of ATR itself and its downstream targets RPA and CHK1.

Reviewed by eLife

Defining amino acid pairs as structural units suggests mutation sensitivity to adjacent residues

1. Aviv A. Rosenberg
2. Nitsan Yehishalom
3. Ailie Marx
4. Alex Bronstein

Reviewed by PREreview

lncRNA read-through regulates the BX-C insulator Fub-1

1. Airat Ibragimov
2. Xin Yang Bing
3. Yulii V Shidlovskii
4. Michael Levine
5. Pavel Georgiev
6. Paul Schedl

• Curated by eLife

  eLife assessment

  This fundamental work substantially advances our understanding of how neighboring genes on a chromosome can be separately controlled in time and space. The evidence supporting the conclusions is compelling, with state-of-the-art genetic perturbations and imaging. The work will be of broad interest to geneticists and cell biologists.

Reviewed by eLife

Inhibition of type I PRMTs reforms muscle stem cell identity enhancing their therapeutic capacity

1. Claudia Dominici
2. Oscar D Villarreal
3. Junio Dort
4. Emilie Heckel
5. Yu Chang Wang
6. Ioannis Ragoussis
7. Jean-Sebastien Joyal
8. Nicolas Dumont
9. Stéphane Richard

• Curated by eLife

  eLife assessment

  This valuable paper informs on the role of type I PRMTs in programming muscle stem cell identification. The evidence presented is mostly solid, with some weaknesses in the evidence regarding the proposed mechanism. The paper will be of particular interest to those who study skeletal muscle satellite cell biology.

Reviewed by eLife, Arcadia Science

CRISPR/Cas9 mediated gene editing in non-model nematode Panagrolaimus sp. PS1159

1. Viktoria Hellekes
2. Denise Claus
3. Johanna Seiler
4. Felix Illner
5. Philipp H. Schiffer
6. Michael Kroiher

Reviewed by Arcadia Science