Molecular Biology

Deficiency of the lipid flippase ATP10A causes diet-induced dyslipidemia in female mice

1. Adriana C. Norris
2. Eugenia M. Yazlovitskaya
3. Lin Zhu
4. Bailey S. Rose
5. Jody C. May
6. Katherine N. Gibson-Corley
7. John A. McLean
8. John M. Stafford
9. Todd R. Graham

Reviewed by PREreview

Molecular basis of interactions between CaMKII and α-actinin-2 that underlie dendritic spine enlargement

1. Ashton J Curtis
2. Jian Zhu
3. Christopher J Penny
4. Matthew G Gold

• Curated by eLife

  eLife assessment

  This fundamental study from Gold and colleagues substantially advances our understanding of the synaptic targeting of a major postsynaptic protein kinase, CaMKII, which is the basis for the persistence of excitatory synaptic strength in synaptic plasticity. The evidence supporting the claims of the authors is convincing, with cell biological, biochemical, as well as structural biological approaches. This work will be of interest to cell and computational biologists working on learning/memory.

Reviewed by eLife

Epigenetic signature of human immune aging in the GESTALT study

1. Roshni Roy
2. Pei-Lun Kuo
3. Julián Candia
4. Dimitra Sarantopoulou
5. Ceereena Ubaida-Mohien
6. Dena Hernandez
7. Mary Kaileh
8. Sampath Arepalli
9. Amit Singh
10. Arsun Bektas
11. Jaekwan Kim
12. Ann Z Moore
13. Toshiko Tanaka
14. Julia McKelvey
15. Linda Zukley
16. Cuong Nguyen
17. Tonya Wallace
18. Christopher Dunn
19. William Wood
20. Yulan Piao
21. Christopher Coletta
22. Supriyo De
23. Jyoti Sen
24. Nan-ping Weng
25. Ranjan Sen
26. Luigi Ferrucci

• Curated by eLife

  eLife assessment

  This is an important study reanalysing previously published datasets to understand methylation changes during aging. The evidence supporting the conclusions is solid and sheds new light on features of aging in cells, highlighting the concept of cell-specific methylation changes and their relationship to other physiological changes such as inflammation that may impact methylation patterns. This work will be of broad interest to cell and molecular biologists.

Reviewed by eLife

A back-door insight into the modulation of Src kinase activity by the polyamine spermidine

1. Sofia Rossini
2. Marco Gargaro
3. Giulia Scalisi
4. Elisa Bianconi
5. Sara Ambrosino
6. Eleonora Panfili
7. Claudia Volpi
8. Ciriana Orabona
9. Antonio Macchiarulo
10. Francesca Fallarino
11. Giada Mondanelli

• Curated by eLife

  eLife assessment

  This is an important study describing the mechanism of Spermidine modulation of Src kinase, identifying the interacting amino acids and the effect on indoleamine 2,3-dioxygenase 1 (IDO1) activation based on solid evidence. Considering the important role of IDO1 in the immune response this study could provide important information for the design of allosteric modulators capable of turning SRC on/off.

Reviewed by eLife

Molecular analysis of binding region of an ACE2 as a receptor for SARS-CoV-2 between humans and mammals

1. Takuma Hayashi
2. Kaoru Aboko
3. Masaki Mandan
4. Nobuo Yaegashi
5. Ikuo Konishi

Reviewed by ScreenIT

SARS-CoV-2 Nsp1 binds the ribosomal mRNA channel to inhibit translation

1. Katharina Schubert
2. Evangelos D. Karousis
3. Ahmad Jomaa
4. Alain Scaiola
5. Blanca Echeverria
6. Lukas-Adrian Gurzeler
7. Marc Leibundgut
8. Volker Thiel
9. Oliver Mühlemann
10. Nenad Ban

Reviewed by ScreenIT

6-Thioguanine blocks SARS-CoV-2 replication by inhibition of PLpro

1. Caleb D. Swaim
2. Varun Dwivedi
3. Yi-Chieh Perng
4. Xu Zhao
5. Larissa A. Canadeo
6. Houda H. Harastani
7. Tamarand L. Darling
8. Adrianus C.M. Boon
9. Deborah J. Lenschow
10. Viraj Kulkarni
11. Jon M. Huibregtse

Reviewed by ScreenIT

A Fast and Accessible Method for the Isolation of RNA, DNA, and Protein To Facilitate the Detection of SARS-CoV-2

1. Jose Carlos Ponce-Rojas
2. Michael S. Costello
3. Duncan A. Proctor
4. Kenneth S. Kosik
5. Maxwell Z. Wilson
6. Carolina Arias
7. Diego Acosta-Alvear

Reviewed by ScreenIT

Conformational dynamics of SARS-CoV-2 trimeric spike glycoprotein in complex with receptor ACE2 revealed by cryo-EM

1. Cong Xu
2. Yanxing Wang
3. Caixuan Liu
4. Chao Zhang
5. Wenyu Han
6. Xiaoyu Hong
7. Yifan Wang
8. Qin Hong
9. Shutian Wang
10. Qiaoyu Zhao
11. Yalei Wang
12. Yong Yang
13. Kaijian Chen
14. Wei Zheng
15. Liangliang Kong
16. Fangfang Wang
17. Qinyu Zuo
18. Zhong Huang
19. Yao Cong

Reviewed by ScreenIT

SARS-CoV-2 Infection of Pluripotent Stem Cell-Derived Human Lung Alveolar Type 2 Cells Elicits a Rapid Epithelial-Intrinsic Inflammatory Response

1. Jessie Huang
2. Adam J. Hume
3. Kristine M. Abo
4. Rhiannon B. Werder
5. Carlos Villacorta-Martin
6. Konstantinos-Dionysios Alysandratos
7. Mary Lou Beermann
8. Chantelle Simone-Roach
9. Jonathan Lindstrom-Vautrin
10. Judith Olejnik
11. Ellen L. Suder
12. Esther Bullitt
13. Anne Hinds
14. Arjun Sharma
15. Markus Bosmann
16. Ruobing Wang
17. Finn Hawkins
18. Eric J. Burks
19. Mohsan Saeed
20. Andrew A. Wilson
21. Elke Mühlberger
22. Darrell N. Kotton

Reviewed by ScreenIT