Molecular Biology

LncRNA Snhg3 aggravates hepatic steatosis via PPARγ signaling

1. Xianghong Xie
2. Mingyue Gao
3. Wei Zhao
4. Chunmei Li
5. Weihong Zhang
6. Jiahui Yang
7. Yinliang Zhang
8. Enhui Chen
9. Yanfang Guo
10. Zeyu Guo
11. Minglong Zhang
12. Ebenezeri Erasto Ngowi
13. Heping Wang
14. Xiaoman Wang
15. Yinghan Zhu
16. Yiting Wang
17. Xiaolu Li
18. Hong Yao
19. Li Yan
20. Fude Fang
21. Meixia Li
22. Aijun Qiao
23. Xiaojun Liu

• Curated by eLife

  eLife assessment

  This study provides useful evidence substantiating a role for long noncoding RNAs in liver metabolism and organismal physiology. Using murine knockout and knock-in models, the authors invoke a previously unidentified role for the lncRNA Snhg3 in fatty liver. The revised manuscript has improved and most studies are backed by solid evidence but the study was found to be incomplete and will require future studies to substantiate some of the claims.

Reviewed by eLife

Fission yeast Caprin protein is required for efficient heterochromatin establishment

1. Haidao Zhang
2. Ekaterina Kapitonova
3. Adriana Orrego
4. Christos Spanos
5. Joanna Strachan
6. Elizabeth H. Bayne

Reviewed by Review Commons

ADAR1 orchestrates the ZBP1-mediated PANoptosis and heart transplant rejection

1. Haitao Lu
2. Jifu Jiang
3. Xuyan Huang
4. Aaron Haig
5. Lakshman Gunaratman
6. Anthony M. Jevnikar
7. Zhu-Xu Zhang

Reviewed by Arcadia Science

Human induced pluripotent stem cell-derived cardiomyocytes to study inflammation-induced aberrant calcium transient

1. Yuki Tatekoshi
2. Chunlei Chen
3. Jason Solomon Shapiro
4. Hsiang-Chun Chang
5. Malorie Blancard
6. Davi M Lyra-Leite
7. Paul W Burridge
8. Matthew Feinstein
9. Richard D'Aquila
10. Priscilla Hsue
11. Hossein Ardehali

• Curated by eLife

  eLife assessment

  This useful study focuses on heart failure with preserved ejection fraction (HFpFE), common in patients with HIV. Researchers used induced human pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) to stimulate HEFpEF) and found that inflammatory cytokines alter Ca2+ transients. SGLT2 inhibitors and mitochondrial antioxidants reversed this effect. While the study is incomplete and preliminary, its strength lies in introducing hiPSC-CMs as a tool for investigating HFpEF mechanisms. A major weakness was found to be limited functional assessment relevant to HFpEF.

Reviewed by eLife

A cryo-electron tomography study of ciliary rootlet organization

1. Chris van Hoorn
2. Andrew P Carter

• Curated by eLife

  eLife assessment

  The study offers a compelling molecular model for the organization of rootlets, a critical organelle that links cilia to the basal body, ensuring proper anchoring. While previous research has explored rootlet structure and organization, this study delivers an unprecedented level of resolution, valuable to the centrosome and cilia field. This research marks a significant step forward in our understanding of rootlets' molecular organization.

Reviewed by eLife

Cryo-EM structure of the CBC-ALYREF complex

1. Bradley P Clarke
2. Alexia E Angelos
3. Menghan Mei
4. Pate S Hill
5. Yihu Xie
6. Yi Ren

• Curated by eLife

  eLife assessment

  This important study reports the cryo-electron microscopy structure of a multi-protein complex that recognizes the 5'-end cap of mRNAs and plays a critical role in mRNA export. The structural and biochemical analyses in this study provide convincing evidence to support the major claims of the authors, with the inclusion of more functional characterizations in cell-based systems having corroborated the claims further and and thus strengthening the study. This paper would be of interest to structural biologists and RNA biologists working on mRNA metabolism.

Reviewed by eLife

The zinc-finger transcription factor Sfp1 imprints specific classes of mRNAs and links their synthesis to cytoplasmic decay

1. Moran Kelbert
2. Antonio Jordán-Pla
3. Lola de Miguel-Jiménez
4. José García-Martínez
5. Michael Selitrennik
6. Adi Guterman
7. Noa Henig
8. Sander Granneman
9. José E Pérez-Ortín
10. Sebastián Chávez
11. Mordechai Choder

• Curated by eLife

  eLife assessment

  This important study reports that a transcription factor stimulating mRNA synthesis can stabilize its target transcripts. The convincing results demonstrate, with multiple independent approaches, co-transcriptional binding, stabilization of a family of mRNAs, and cytoplasmic activities of the transcription factor Sfp1. The results lead to the conclusion that the co-transcriptional association of Sfp1 with specific transcripts is a critical step in the stabilization of such transcripts in the cytoplasm.

Reviewed by eLife

Productive mRNA Chromatin Escape is Promoted by PRMT5 Methylation of SNRPB

1. Joseph D. DeAngelo
2. Maxim I. Maron
3. Jacob S. Roth
4. Aliza M. Silverstein
5. Varun Gupta
6. Stephanie Stransky
7. Joel Basken
8. Joey Azofeifa
9. Simone Sidoli
10. Matthew J. Gamble
11. David Shechter

Reviewed by Arcadia Science

Non-canonical activation of IRE1α during Candida albicans infection enhances macrophage fungicidal activity

1. Michael J. McFadden
2. Mack B. Reynolds
3. Britton C Michmerhuizen
4. Einar B. Ólafsson
5. Faith M. Anderson
6. Tracey L. Schultz
7. Mary X.D. O’Riordan
8. Teresa R. O’Meara

Reviewed by Review Commons

PRC2 promotes canalisation during endodermal differentiation

1. Jurriaan Jochem Hölzenspies
2. Dipta Sengupta
3. Wendy Anne Bickmore
4. Joshua Mark Brickman
5. Robert Scott Illingworth

Reviewed by Review Commons