Molecular Biology

  1. Scaling of an antibody validation procedure enables quantification of antibody performance in major research applications

    1. Riham Ayoubi
    2. Joel Ryan
    3. Michael S Biddle
    4. Walaa Alshafie
    5. Maryam Fotouhi
    6. Sara Gonzalez Bolivar
    7. Vera Ruiz Moleon
    8. Peter Eckmann
    9. Donovan Worrall
    10. Ian McDowell
    11. Kathleen Southern
    12. Wolfgang Reintsch
    13. Thomas M Durcan
    14. Claire Brown
    15. Anita Bandrowski
    16. Harvinder Virk
    17. Aled M Edwards
    18. Peter McPherson
    19. Carl Laflamme

    • Curated by eLife

      eLife assessment

      Antibodies are some of the most critical tools in biomedical research. However, their quality and specificity vary significantly. This fundamental study provides guidelines for how the quality of an antibody should be assessed and recorded and provides compelling data on the selected antibodies. This paper will be of interest to researchers working in experimental cell biology.

    Reviewed by eLife

    4 evaluationsAppears in 1 listLatest version Latest activity

  2. Rapid cloning-free mutagenesis of new SARS-CoV-2 variants using a novel reverse genetics platform

    1. Enja Tatjana Kipfer
    2. David Hauser
    3. Martin J Lett
    4. Fabian Otte
    5. Lorena Urda
    6. Yuepeng Zhang
    7. Christopher MR Lang
    8. Mohamed Chami
    9. Christian Mittelholzer
    10. Thomas Klimkait

    • Curated by eLife

      eLife assessment

      This study describes CLEVER, an improved method for fast and efficient rescue and mutagenesis of SARS-CoV2. While the principle of this method is not new, this work significantly improves upon existing protocols, providing an important advancement in the field of viral infectious clones. Convincing proof-of-concept experiments were performed that demonstrate the utility and efficiency of the method.

    Reviewed by eLife

    6 evaluationsAppears in 1 listLatest version Latest activity

  3. Inhibiting Glutamine Metabolism Blocks Coronavirus Replication in Mammalian Cells

    1. Kai Su Greene
    2. Annette Choi
    3. Matthew Chen
    4. Nianhui Yang
    5. Ruizhi Li
    6. Yijian Qiu
    7. Michael J Lukey
    8. Katherine S Rojas
    9. Jonathan Shen
    10. Kristin F Wilson
    11. William P Katt
    12. Gary R Whittaker
    13. Richard A Cerione

    Reviewed by Rapid Reviews Infectious Diseases

    3 evaluationsAppears in 1 listLatest version Latest activity

  4. The long non-coding RNA LINC00941 modulates MTA2/NuRD occupancy to suppress premature human epidermal differentiation

    1. Eva Morgenstern
    2. Uwe Schwartz
    3. Johannes Graf
    4. Astrid Bruckmann
    5. Markus Kretz

    Reviewed by Review Commons

    4 evaluationsAppears in 1 listLatest version Latest activity

  5. TAX1BP1 recruits ATG9 vesicles through SCAMP3 binding

    1. Yutaro Hama
    2. Yoshitaka Kurikawa
    3. Takahide Matsui
    4. Noboru Mizushima
    5. Hayashi Yamamoto

    Reviewed by Review Commons

    4 evaluationsAppears in 1 listLatest version Latest activity

  6. Multiple cullin-associated E3 ligases regulate cyclin D1 protein stability

    1. Ke Lu
    2. Ming Zhang
    3. Guizheng Wei
    4. Guozhi Xiao
    5. Liping Tong
    6. Di Chen

    • Curated by eLife

      eLife assessment

      This paper is of interest to cell biologists studying the mechanisms of protein posttranslational modifications. The study investigates Cullin-RING ubiquitin E3 ligases (CRLs) that regulate cyclin D1 protein stability in cells by utilizing siRNA screening and ectopic expression approaches. By screening a siRNA library containing different E3 ligases, the authors identified a previously uncharacterized combination of Cullin 1-7 and associated E3 ligases (Keap1-CUL3, DDB2-CUL4A/4B, WSB2-CUL2/5, and RBX1-CUL1-7) that are important for cyclin D1 ubiquitination and proteasome-mediated degradation.

    Reviewed by eLife

    5 evaluationsAppears in 1 listLatest version Latest activity

  7. Tardigrades dramatically upregulate DNA repair pathway genes in response to ionizing radiation

    1. Courtney M. Clark-Hachtel
    2. Jonathan D. Hibshman
    3. Tristan De Buysscher
    4. Bob Goldstein

    Reviewed by preLights

    1 evaluationAppears in 1 listLatest version Latest activity

  8. ZMYM2 controls human transposable element transcription through distinct co-regulatory complexes

    1. Danielle J Owen
    2. Elisa Aguilar-Martinez
    3. Zongling Ji
    4. Yaoyong Li
    5. Andrew D Sharrocks

    Reviewed by eLife

    13 evaluationsAppears in 1 listLatest version Latest activity

  9. Transcription-independent hold of the G1/S transition is exploited to cope with DNA replication stress

    1. Yue Jin
    2. Guoqing Lan
    3. Jiaxin Zhang
    4. Haoyuan Sun
    5. Li Xin
    6. Qinhong Cao
    7. Chao Tang
    8. Xiaojing Yang
    9. Huiqiang Lou
    10. Wenya Hou

    Reviewed by Review Commons

    8 evaluationsAppears in 1 listLatest version Latest activity

  10. Non-canonical contribution of human Dicer helicase domain in antiviral innate immune response

    1. Morgane Baldaccini
    2. Léa Gaucherand
    3. Béatrice Chane-Woon-Ming
    4. Mélanie Messmer
    5. Floriane Gucciardi
    6. Sébastien Pfeffer

    Reviewed by Review Commons

    4 evaluationsAppears in 1 listLatest version Latest activity
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