Molecular Biology

Neuralized-like proteins differentially activate Notch ligands

1. Alina Airich
2. Oren Gozlan
3. Ekaterina Seib
4. Gittel Leah Shaingarten
5. Lena-Sophie Wilschrey
6. Liora Lindenboim
7. David Sprinzak
8. Thomas Klein

Reviewed by Review Commons

Tom1p ubiquitin ligase structure, interaction with Spt6p, and function in maintaining normal transcript levels and the stability of chromatin in promoters

1. Jennifer Madrigal
2. Heidi L Schubert
3. Matthew A Sdano
4. Laura McCullough
5. Zaily Connell
6. Tim Formosa
7. Christopher P Hill

• Curated by eLife

  eLife Assessment

  This useful manuscript presents findings on Tom1p's interaction with Spt6p and its role in chromatin dynamics, supported by structural analysis through CryoEM. The evidence for the conclusions is currently incomplete, lacking key experiments including continuation in vivo interaction and orthogonal binding assays (e.g., SPR, MST, ITC) to fully support the proposed mechanism. While the results are promising, further validation is needed to strengthen the evidence and improve the manuscript's overall cohesion.

Reviewed by eLife