Molecular Biology

Optogenetic control of Protein Kinase C-epsilon activity reveals its intrinsic signaling properties with spatiotemporal resolution

1. Qunxiang Ong
2. Crystal Jing Yi Lim
3. Yilie Liao
4. Justin Tze-Yang Ng
5. Ler Ting Rachel Lim
6. Shernys Xuan Yi Koh
7. Sher En Chan
8. Pheobe Lee Yu Ying
9. Huijun Lim
10. Chen Rui Ye
11. Loo Chien Wang
12. Siok Ghee Ler
13. Radoslaw M Sobota
14. Yaw Sing Tan
15. Gerald I Shulman
16. Xiaoyong Yang
17. Weiping Han

• Curated by eLife

  eLife Assessment

  This manuscript introduces Opto-PKCε, an optogenetic tool that enabled important findings derived from interactome and phosphoproteome studies. Light-dependent recruitment of Opto-PKCε to the plasma membrane revealed the specific phosphorylation of the insulin receptor at Thr 1160. In turn, recruitment to mitochondria led to phosphorylation of the complex I subunit NDUFS4, correlating with reduced spare respiratory capacity. The evidence supporting these conclusions is solid, although additional clarification on data analysis would further enhance readability.

Reviewed by eLife

Transcriptomic meta-analysis in plaque psoriasis: an integrative bioinformatic approach to deciphering the genetic landscape and molecular pathways

1. Teresa Torres-Moral
2. Josep Riera-Monroig
3. Gemma Tell-Martí
4. Jaume Bagué
5. José F. Català-Senent
6. Francisco J. Roig
7. Míriam Potrony
8. Francisco García-García
9. Susana Puig

Reviewed by Arcadia Science

The SUMO activating enzyme subunit, SAE2, contributes SUMO protein bias for mitotic fidelity

1. Alexandra K. Walker
2. Alexander J. Lanz
3. Mohammed Jamshad
4. Alexander J. Garvin
5. Peter Wotherspoon
6. Benjamin F. Cooper
7. Timothy J. Knowles
8. Joanna R. Morris

Reviewed by Review Commons