Molecular Biology

Brown Adipose Tissue and Skeletal Muscle Coordinately Contribute to Thermogenesis in Mice

1. Yuna Izumi-Mishima
2. Rie Tsutsumi
3. Tetsuya Shiuchi
4. Saori Fujimoto
5. Momoka Taniguchi
6. Yuko Okamatsu-Ogura
7. Takeshi Yoneshiro
8. Masashi Kuroda
9. Kazuhiro Nomura
10. Hiroshi Sakaue

• Curated by eLife

  eLife assessment

  This is a useful paper regarding the roles of brown adipose tissue and skeletal muscle in thermogenesis in mice, with potential significance for the field. The overall approach is innovative but on balance the evidence for the claim is incomplete, as cast immobilization, while innovative, is likely stressful, may impact muscle and BAT directly, and imposes an energetic cost of motion on the animal that is not accounted for. Further experiments are also needed to directly assess the role of adipose-derived BCAAs in thermogenesis.

Reviewed by eLife

An intestinal Sir2-HSF1-ATGL1 pathway regulates lipolysis in C. elegans

1. Milán Somogyvári
2. Saba Khatatneh
3. Gábor Hajdú
4. Bar Sotil
5. József Murányi
6. Csaba Sőti

Reviewed by Review Commons

An in vitro approach reveals molecular mechanisms underlying endocrine disruptor-induced epimutagenesis

1. Jake D Lehle
2. Yu-Huey Lin
3. Amanda Gomez
4. Laura Chavez
5. John R McCarrey

• Curated by eLife

  eLife assessment

  This important study, characterizing the epigenetic and transcriptomic response of a variety of cell types representative of somatic, germline, and pluripotent cells to BPS, reveals the cell type-specific changes in DNA methylation and the relationship with the genome sequence. The findings are convincing and provide a basis for future analyses in vivo. This work should be of interest to biomedical researchers who work on epigenetic reprogramming and epigenetic inheritance.

Reviewed by eLife

Epigenetic delineation of the earliest cardiac lineage segregation by single-cell multi-omics

1. Peng Xie
2. Xu Jiang
3. Jingjing He
4. Qingyun Pan
5. Xianfa Yang
6. Yanying Zheng
7. Wenli Fan
8. Chen Wu
9. Weiheng Zheng
10. Ke Fang
11. Shuhan Si
12. Shiqi Zhu
13. Yi Yang
14. Tao P Zhong
15. Zhongzhou Yang
16. Ke Wei
17. Wei Xie
18. Naihe Jing
19. Zhuojuan Luo
20. Chengqi Lin

• Curated by eLife

  eLife assessment

  This study combines extensive published and new datasets to provide a useful single-cell multi-omics analysis of early cardiac lineage segregation, highlighting the mutual regulation of key regulators for cardiac specification. While the data presentation is robust, the computational methods for delineating cardiac lineage trajectories and the functional analyses are incomplete and require further clarification and additional experiments. If validated, these findings will be of significant interest to researchers in the fields of cardiac development and congenital heart disease.

Reviewed by eLife

A fine kinetic balance of interactions directs transcription factor hubs to genes

1. Samantha Fallacaro
2. Apratim Mukherjee
3. Puttachai Ratchasanmuang
4. Joseph Zinski
5. Yara I Haloush
6. Kareena Shankta
7. Mustafa Mir

Reviewed by preLights

ACE2-independent sarbecovirus cell entry is supported by TMPRSS2-related enzymes and reduces sensitivity to antibody-mediated neutralization

1. Lu Zhang
2. Hsiu-Hsin Cheng
3. Nadine Krüger
4. Bojan Hörnich
5. Luise Graichen
6. Alexander S. Hahn
7. Sebastian R. Schulz
8. Hans-Martin Jäck
9. Metodi V. Stankov
10. Georg M.N. Behrens
11. Marcel A. Müller
12. Christian Drosten
13. Onnen Mörer
14. Martin Sebastian Winkler
15. ZhaoHui Qian
16. Stefan Pöhlmann
17. Markus Hoffmann

Reviewed by Review Commons

Multi-protein chimeric antigens, a novel combined approach for efficiently targeting and blocking the blood stage of Plasmodium falciparum

1. Bhagyashree Deshmukh
2. Dhruv Khatri
3. Sanjay Kumar Kochar
4. Chaitanya Athale
5. Krishanpal Karmodiya

Reviewed by Review Commons

Association with TFIIIC limits MYCN localisation in hubs of active promoters and chromatin accumulation of non-phosphorylated RNA polymerase II

1. Raphael Vidal
2. Eoin Leen
3. Steffi Herold
4. Mareike Müller
5. Daniel Fleischhauer
6. Christina Schülein-Völk
7. Dimitrios Papadopoulos
8. Isabelle Röschert
9. Leonie Uhl
10. Carsten P Ade
11. Peter Gallant
12. Richard Bayliss
13. Martin Eilers
14. Gabriele Büchel

• Curated by eLife

  eLife assessment

  This study presents the valuable finding that TFIIIC interacts with MYCN to regulate RNA polymerase II dynamics by dissecting its impact on 3D chromatin architecture. Authors provide convincing evidence that MYCN and TFIIIC show long-range chromatin contacts, and that the expression of each protein limits the function of the other. The notion emerges that TFIIIC helps MYCN to maintain output at promoters while decreasing less productive associations at larger more extensively connected chromatin hubs. The paper is of interest to molecular biologists working on MYCN-dependent regulation of gene expression.

Reviewed by eLife

Comprehensive Phenotyping of Extracellular Vesicles in Blood of Healthy Humans – Insights into Cellular Origin and Biological Variability

1. Marija Holcar
2. Ivica Marić
3. Tobias Tertel
4. Katja Goričar
5. Urška Čegovnik Primožič
6. Darko Černe
7. Bernd Giebel
8. Metka Lenassi

Reviewed by Arcadia Science

Deciphering the actin structure-dependent preferential cooperative binding of cofilin

1. Kien Xuan Ngo
2. Huong T Vu
3. Kenichi Umeda
4. Minh-Nhat Trinh
5. Noriyuki Kodera
6. Taro Uyeda

• Curated by eLife

  eLife assessment

  In this manuscript the authors present high-speed atomic force microscopy (HSAFM) to analyze real-time structural changes in actin filaments induced by cofilin binding. This important study enhances our understanding of actin dynamics which plays a crucial role in a broad spectrum of cellular activities based on solid experimental evidence. Some technical questions, however, remain, making the data interpretation incomplete.

Reviewed by eLife