Molecular Biology

Gle1 is required for tRNA to stimulate Dbp5 ATPase activity in vitro and promote Dbp5-mediated tRNA export in vivo in Saccharomyces cerevisiae

1. Arvind Arul Nambi Rajan
2. Ryuta Asada
3. Ben Montpetit

• Curated by eLife

  eLife assessment

  The work is a valuable contribution to understanding the mechanism of nuclear export of tRNA in budding yeast. The authors present solid evidence that Dbp5 functions in parallel with Los1 and Msn5 in tRNA export, in a manner dependent on Gle1 for activation of its ATPase activity but independently of Mex67, Dbp5's partner in mRNA export. It further presents biochemical evidence that Dbp5 can bind tRNA but that Gle1 and InsP6 are required for activating ATP hydrolysis by the Dbp5-tRNA complex, suggesting a possible mechanism for tRNA export by Dbp5.

Reviewed by eLife

Protein Kinase Structure and Dynamics: Role of the αC-β4 Loop

1. Jian Wu
2. Nisha A. Jonniya
3. Sophia P. Hirakis
4. Cristina Olivieri
5. Gianluigi Veglia
6. Alexandr P. Kornev
7. Susan S. Taylor

• Curated by eLife

  eLife assessment

  This valuable study draws attention to the importance of a previously overlooked structural motif in kinase regulation. While the data presented are intriguing and mostly solid, further analysis and additional experiments will be needed in the future to support the authors' hypothesis. The work will be of interest to protein biochemists and enzymologists with an interest in kinases and allostery.

Reviewed by eLife

Deep quantitative glycoproteomics reveals gut microbiome induced remodeling of the brain glycoproteome

1. Clément M Potel
2. Mira Lea Burtscher
3. Martin Garrido-Rodriguez
4. Amber Brauer-Nikonow
5. Isabelle Becher
6. Athanasios Typas
7. Michael Zimmermann
8. Mikhail M Savitski

Reviewed by PREreview

Adenine methylation is very scarce in the Drosophila genome and not erased by the ten-eleven translocation dioxygenase

1. Manon Boulet
2. Guerric Gilbert
3. Yoan Renaud
4. Martina Schmidt-Dengler
5. Emilie Plantié
6. Romane Bertrand
7. Xinsheng Nan
8. Tomasz Jurkowski
9. Mark Helm
10. Laurence Vandel
11. Lucas Waltzer

• Curated by eLife

  eLife assessment

  This study investigates the presence of DNA adenine methylation (6mA) and the associated function of TET enzyme, a DNA methylation mark eraser, in Drosophila. The study presents valuable findings on the scarcity of 6mA in the Drosophila genome and challenges previous findings regarding the role of TET in 6mA modification. The evidence supporting the claims is solid, and the paper has the potential to stimulate re-evaluations of the significance and regulatory mechanisms of 6mA DNA modifications in Drosophila.

Reviewed by eLife

A SUMO E3 ligase promotes long non-coding RNA transcription to regulate small RNA-directed DNA elimination

1. Salman Shehzada
2. Tomoko Noto
3. Julie Saksouk
4. Kazufumi Mochizuki

Reviewed by Review Commons

Conserved and repetitive motifs in an intrinsically disordered protein drive α-carboxysome assembly

1. Julia B. Turnšek
2. Luke M. Oltrogge
3. David F. Savage

Reviewed by Arcadia Science

Hibernating ribosomes tether to mitochondria as an adaptive response to cellular stress during glucose depletion

1. Olivier Gemin
2. Maciej Gluc
3. Michael Purdy
4. Higor Rosa
5. Moritz Niemann
6. Yelena Peskova
7. Simone Mattei
8. Ahmad Jomaa

Reviewed by preLights

RIOK2 phosphorylation by RSK promotes synthesis of the human small ribosomal subunit

1. Emilie L. Cerezo
2. Thibault Houles
3. Oriane Lié
4. Marie-Kerguelen Sarthou
5. Charlotte Audoynaud
6. Geneviève Lavoie
7. Maral Halladjian
8. Sylvain Cantaloube
9. Carine Froment
10. Odile Burlet-Schiltz
11. Yves Henry
12. Philippe P. Roux
13. Anthony K. Henras
14. Yves Romeo

Reviewed by Review Commons

SUMOylation of Bonus, the Drosophila homolog of Transcription Intermediary Factor 1, safeguards germline identity by recruiting repressive chromatin complexes to silence tissue-specific genes

1. Baira Godneeva
2. Maria Ninova
3. Katalin Fejes-Toth
4. Alexei Aravin

• Curated by eLife

  eLife assessment

  This important study advances our knowledge of Drosophila Bonus, the sole ortholog of the mammalian transcriptional regulator Tif1. Solid evidence, both in vivo and in vitro, shows how SUMOylation controls the function of the Bonus protein and what the impact of SUMOylation on the function of Bonus protein in the ovary is.

Reviewed by eLife

Enrichment of rare codons at 5' ends of genes is a spandrel caused by evolutionary sequence turnover and does not improve translation

1. Richard Sejour
2. Janet Leatherwood
3. Alisa Yurovsky
4. Bruce Futcher

• Curated by eLife

  eLife assessment

  This is an important contribution to the origins and translational consequences of the relatively low rate of translation elongation in the first ∼30-50 codons of genes in most organisms. The authors provide convincing evidence that the prevalence of rare codons in the first ~40 codons in yeast is due to the relatively recent evolution of these coding sequences, or of lower purifying selection operating on them, and that a preponderance of codons encoded by rare tRNAs near the N-terminus is not associated with higher translational efficiency in the manner proposed by the "translational ramp" hypothesis. The work is incomplete in that the results of reporter assays may have been confounded by alterations of mRNA sequence or structure that could have influenced their translation or mRNA stability; that the work cannot fully account for a greater enrichment of slowly translated codons in N-terminal vs. C-terminal regions; and that the work does not resolve whether translation elongation through N-terminal coding is truly slow.

Reviewed by eLife