Microbiology

Leaffooted bugs enrich local soil with their horizontally acquired symbiont

1. Bibek Singh Parajuli
2. John Teodosio
3. Alison Ravenscraft

Reviewed by Arcadia Science

Interferon-β induction heterogeneity during KSHV infection is correlated to levels and activation of the transcription factors ATF2 and RelA, and not IRF3

1. Machika Kaku
2. Marta Maria Gaglia

Reviewed by Review Commons

Detecting, mapping, and suppressing the spread of a decade-long Pseudomonas aeruginosa nosocomial outbreak with genomics

1. William Stribling
2. Lindsey R Hall
3. Aubrey Powell
4. Casey Harless
5. Melissa J Martin
6. Brendan W Corey
7. Erik Snesrud
8. Ana Ong
9. Rosslyn Maybank
10. Jason Stam
11. Katelyn V Bartlett
12. Brendan T Jones
13. Lan N Preston
14. Katherine F Lane
15. Bernadette Thompson
16. Lynn M Young
17. Yoon I Kwak
18. Alice E Barsoumian
19. Ana Elizabeth Markelz
20. John L Kiley
21. Robert J Cybulski
22. Jason W Bennett
23. Patrick T Mc Gann
24. Francois Lebreton

• Curated by eLife

  eLife Assessment

  This important work presents an example of how genomic data can be used to improve understanding of an ongoing, long-term bacterial outbreak in a hospital with an application to multi-drug resistant Pseudomonas aeruginosa, and will be of interest to researchers concerned with the spread of drug-resistant bacteria in hospital settings. The convincing genomic analyses highlight the value of routine surveillance of patients and environmental sampling and show how such data can help in dating the origin of the outbreak and in characterising the epidemic lineages. These findings highlight the importance of understanding environmental factors contributing to the transmission of P. aeruginosa for guiding and tailoring infection control efforts.

Reviewed by eLife

The glycoprotein quality control factor Malectin promotes coronavirus replication and viral protein biogenesis

1. Jonathan P Davies
2. Lars Plate

• Curated by eLife

  eLife Assessment

  This is an important study that utilizes proteomic and genetic approaches to identify the glycoprotein quality control factor malectin as a pro-viral host protein involved in the replication of coronavirus. The evidence supporting this conclusion is convincing, although continued elucidation of the mechanistic basis of malectin-mediated viral replication would further strengthen these findings. This work will be of interest to cell biologists studying the molecular mechanisms of glycoprotein quality control and virologists studying the host-pathogen interactions.

Reviewed by eLife

Neutralizing human monoclonal antibodies that target the PcrV component of the Type III Secretion System of Pseudomonas aeruginosa act through distinct mechanisms

1. Jean-Mathieu Desveaux
2. Eric Faudry
3. Carlos Contreras-Martel
4. François Cretin
5. Leonardo Sebastian Dergan-Dylon
6. Axelle Amen
7. Isabelle Bally
8. Victor TardivyCasemajor
9. Fabien Chenavier
10. Delphine Fouquenet
11. Yvan Caspar
12. Ina Attrée
13. Andréa Dessen
14. Pascal Poignard

• Curated by eLife

  eLife Assessment

  This useful work identifies new monoclonal antibodies produced by cystic fibrosis patients against Pseudomonas aeruginosa type three secretion system. The evidence supporting authors' claim is solid. Nonetheless, the manuscript may benefit from a more in depth description of what the authors learned from their structure-based analyses of antibodies targeting PcrV.

Reviewed by eLife

Non-destructive label-free automated identification of bacterial colonies at the species level directly on agar media using digital holography and convolutional neural network algorithms

1. Prisca Perlemoine
2. Jordan Belissard
3. Bruno Burtschell
4. Nassim Halli
5. Luc Martin
6. Camille Brunet
7. Maxime Gougis
8. Patrick Schiavone
9. Yvan Caspar

Reviewed by Arcadia Science

Provision of Preferred Nutrients to Macrophages Enables Salmonella to Replicate Intracellularly Without Relying on Type III Secretion Systems

1. Francisco-Javier Garcia-Rodriguez
2. Camila Valenzuela
3. Joaquin Bernal-Bayard
4. Pedro Escoll

Reviewed by Review Commons

Resource landscape shapes the composition and stability of the human vaginal microbiota

1. Tsukushi Kamiya
2. Mircea T. Sofonea
3. Michael France
4. Nicolas Tessandier
5. Ignacio G Bravo
6. Carmen Lia Murall
7. Jacques Ravel
8. Samuel Alizon

Reviewed by Peer Community in Ecology

Applying 3D correlative structured illumination microscopy and X-ray tomography to characterise herpes simplex virus-1 morphogenesis

1. Kamal L Nahas
2. Viv Connor
3. Kaveesha J Wijesinghe
4. Henry G Barrow
5. Ian M Dobbie
6. Maria Harkiolaki
7. Stephen C Graham
8. Colin M Crump

• Curated by eLife

  eLife Assessment

  This landmark manuscript comprehensively examines the roles of nine structural proteins in herpes simplex virus 1 (HSV-1) assembly and nuclear egress. By integrating cryo-light microscopy and soft X-ray tomography, the study presents an innovative approach to investigating viral assembly within cells. The research is thoroughly executed, yielding exceptional data that explain previously unknown functions expected to bear widespread influence. This work is of broad interest to virologists, cellular biologists, and structural biologists, offering a robust, contextually rich methodology for studying large protein complex assembly within the cellular environment, serving as an excellent starting point for high-resolution techniques.

Reviewed by eLife

The increase in cell volume and nuclear number of the koji-fungus Aspergillus oryzae contributes to its high enzyme productivity

1. Ayaka Itani
2. Haruto Motomura
3. Ken Oda
4. Hideyuki Yamashita
5. Kanae Sakai
6. Ken-ichi Kusumoto
7. Shinsuke Shigeto
8. Takehiko Ichikawa
9. Hosain Mohammad Mubarak
10. Takeshi Fukuma
11. Takuya Katayama
12. Jun-ichi Maruyama
13. Shunsuke Masuo
14. Naoki Takaya
15. Norio Takeshita

• Curated by eLife

  eLife Assessment

  The ratio of nuclei to cell volume is a well-controlled parameter in eukaryotic cells. This study now reports important findings that expand our understanding of the regulatory relationship between cell size and number of nuclei. The evidence supporting the conclusions is convincing obtained by applying appropriate and validated methodology in line with current state-of-the-art. The paper will be of broad interest for cell biologists and fungal biotechnologists seeking to understand mechanisms determining cell size and number of nuclei and why this knowledge might also be of importance for the production of enzymes and thus production strains not only of Aspergillus oryzae but also other industrially used fungi.

Reviewed by eLife