Microbiology

The Shigella flexneri effector IpaH1.4 facilitates RNF213 degradation and protects cytosolic bacteria against interferon-induced ubiquitylation

1. Luz Saavedra-Sanchez
2. Mary S Dickinson
3. Shruti S Apte
4. Yifeng Zhang
5. Maarten De Jong
6. Samantha Skavicus
7. Nicholas S Heaton
8. Neal M Alto
9. Jorn Coers

• Curated by eLife

  eLife Assessment

  In this manuscript, the authors report the fundamental finding that a secreted ubiquitin ligase of Shigella, called IpaH1.4, mediates the degradation of a host defense factor, RNF213. The data are convincing and represent a major contribution to our understanding of cell-autonomous immunity and bacterial pathogenesis as they provide new mechanistic insight into how the cytosolic bacterial pathogen Shigella flexneri evades IFN-induced host immunity.

Reviewed by eLife

The vaccine candidate Liver Stage Antigen 3 is exported during Plasmodium falciparum infection and required for liver-stage development

1. Robyn McConville
2. Ryan WJ Steel
3. Matthew T O’Neill
4. Alan F Cowman
5. Norman Kneteman
6. Justin A Boddey

• Curated by eLife

  eLife Assessment

  This useful study provides new insights into the liver stage antigen LSA3, its export to erythrocytes, and its role in liver stage development. While the functional importance of LSA3 is well-demonstrated, the data underlying conclusions about antibody specificity, liver stage localization, and phenotype remain incomplete. A key gain is the use of mosquito and humanized mouse models to access life cycle stages rarely studied in most laboratories.

Reviewed by eLife

Leaffooted bugs enrich local soil with their horizontally acquired symbiont

1. Bibek Singh Parajuli
2. John Teodosio
3. Alison Ravenscraft

Reviewed by Arcadia Science

Interferon-β induction heterogeneity during KSHV infection is correlated to levels and activation of the transcription factors ATF2 and RelA, and not IRF3

1. Machika Kaku
2. Marta Maria Gaglia

Reviewed by Review Commons

Detecting, mapping, and suppressing the spread of a decade-long Pseudomonas aeruginosa nosocomial outbreak with genomics

1. William Stribling
2. Lindsey R Hall
3. Aubrey Powell
4. Casey Harless
5. Melissa J Martin
6. Brendan W Corey
7. Erik Snesrud
8. Ana Ong
9. Rosslyn Maybank
10. Jason Stam
11. Katelyn V Bartlett
12. Brendan T Jones
13. Lan N Preston
14. Katherine F Lane
15. Bernadette Thompson
16. Lynn M Young
17. Yoon I Kwak
18. Alice E Barsoumian
19. Ana Elizabeth Markelz
20. John L Kiley
21. Robert J Cybulski
22. Jason W Bennett
23. Patrick T Mc Gann
24. Francois Lebreton

• Curated by eLife

  eLife Assessment

  This important work presents an example of how genomic data can be used to improve understanding of an ongoing, long-term bacterial outbreak in a hospital with an application to multi-drug resistant Pseudomonas aeruginosa, and will be of interest to researchers concerned with the spread of drug-resistant bacteria in hospital settings. The convincing genomic analyses highlight the value of routine surveillance of patients and environmental sampling and show how such data can help in dating the origin of the outbreak and in characterising the epidemic lineages. These findings highlight the importance of understanding environmental factors contributing to the transmission of P. aeruginosa for guiding and tailoring infection control efforts.

Reviewed by eLife

The glycoprotein quality control factor Malectin promotes coronavirus replication and viral protein biogenesis

1. Jonathan P Davies
2. Lars Plate

• Curated by eLife

  eLife Assessment

  This is an important study that utilizes proteomic and genetic approaches to identify the glycoprotein quality control factor malectin as a pro-viral host protein involved in the replication of coronavirus. The evidence supporting this conclusion is convincing, although continued elucidation of the mechanistic basis of malectin-mediated viral replication would further strengthen these findings. This work will be of interest to cell biologists studying the molecular mechanisms of glycoprotein quality control and virologists studying the host-pathogen interactions.

Reviewed by eLife

Neutralizing human monoclonal antibodies that target the PcrV component of the Type III Secretion System of Pseudomonas aeruginosa act through distinct mechanisms

1. Jean-Mathieu Desveaux
2. Eric Faudry
3. Carlos Contreras-Martel
4. François Cretin
5. Leonardo Sebastian Dergan-Dylon
6. Axelle Amen
7. Isabelle Bally
8. Victor TardivyCasemajor
9. Fabien Chenavier
10. Delphine Fouquenet
11. Yvan Caspar
12. Ina Attrée
13. Andréa Dessen
14. Pascal Poignard

• Curated by eLife

  eLife Assessment

  This useful work identifies new monoclonal antibodies produced by cystic fibrosis patients against Pseudomonas aeruginosa type three secretion system. The evidence supporting authors' claim is solid. Nonetheless, the manuscript may benefit from a more in depth description of what the authors learned from their structure-based analyses of antibodies targeting PcrV.

Reviewed by eLife

Non-destructive label-free automated identification of bacterial colonies at the species level directly on agar media using digital holography and convolutional neural network algorithms

1. Prisca Perlemoine
2. Jordan Belissard
3. Bruno Burtschell
4. Nassim Halli
5. Luc Martin
6. Camille Brunet
7. Maxime Gougis
8. Patrick Schiavone
9. Yvan Caspar

Reviewed by Arcadia Science

Provision of Preferred Nutrients to Macrophages Enables Salmonella to Replicate Intracellularly Without Relying on Type III Secretion Systems

1. Francisco-Javier Garcia-Rodriguez
2. Camila Valenzuela
3. Joaquin Bernal-Bayard
4. Pedro Escoll

Reviewed by Review Commons

Resource landscape shapes the composition and stability of the human vaginal microbiota

1. Tsukushi Kamiya
2. Mircea T. Sofonea
3. Michael France
4. Nicolas Tessandier
5. Ignacio G Bravo
6. Carmen Lia Murall
7. Jacques Ravel
8. Samuel Alizon

Reviewed by Peer Community in Ecology