Microbiology

A connection between Vibrio cholerae motility and inter-animal transmission

1. Ian W. Campbell
2. Ruchika Dehinwal
3. Alexander A. Morano
4. Katherine G. Dailey
5. Franz G. Zingl
6. Matthew K. Waldor

Reviewed by PREreview

Global transcription factors analyses reveal hierarchy and synergism of regulatory networks and master virulence regulators in Pseudomonas aeruginosa

1. Jiadai Huang
2. Yue Sun
3. Fang Chen
4. Shumin Li
5. Liangliang Han
6. Jingwei Li
7. Zhe He
8. Canfeng Hua
9. Chunyan Yao
10. Tianmin Li
11. Beifang Lu
12. Yung-Fu Chang
13. Xin Deng

• Curated by eLife

  eLife Assessment

  This study provides a valuable and comprehensive dataset on transcription factor binding in Pseudomonas aeruginosa, along with analyses of its regulatory network, key virulence and metabolic regulators, and a pangenomic examination of transcription factors. Utilizing large-scale ChIP-seq and multi-omics integration, the research convincingly supports the hierarchical regulatory structures and offers insights into virulence mechanisms. While further experimental validation is needed, this publicly accessible PATF_Net database enhances its utility for researchers investigating this significant pathogen associated with hospital infections and antibiotic resistance.

Reviewed by eLife

Prodrug activation in malaria parasites mediated by an imported erythrocyte esterase, acylpeptide hydrolase (APEH)

1. Sesh A. Sundararaman
2. Justin J. Miller
3. Ellora C. Daley
4. Kelsey A. O’Brien
5. Paulina Kasak
6. Abigail M. Daniels
7. Rachel L. Edwards
8. Kenneth M. Heidel
9. Darean A. Bague
10. Madeleine A. Wilson
11. Andrew J. Koelper
12. Elexi C. Kourtoglou
13. Alex D. White
14. Sloan A. August
15. Georgia A. Apple
16. Regis W. Rouamba
17. Anthony J. Durand
18. John J. Esteb
19. Florian L. Muller
20. R. Jeremy Johnson
21. Geoffrey C. Hoops
22. Cynthia S. Dowd
23. Audrey R. Odom John

Reviewed by Rapid Reviews Infectious Diseases

Chalkophore-mediated respiratory oxidase flexibility controls M. tuberculosis virulence

1. John A Buglino
2. Yaprak Ozakman
3. Chad E Hatch
4. Anna Benjamin
5. Derek S Tan
6. Michael S Glickman

• Curated by eLife

  eLife Assessment

  In this important study, the authors advance our understanding of copper uptake by chalkophores and their targeted metalloproteins in Mycobacterium tuberculosis. These convincing data demonstrate that chalkophore-acquired copper is solely incorporated into the Mtb bcc:aa3 copper-iron respiratory oxidase under low copper conditions, and that chalkophore-mediated protection of the respiratory chain is critical to Mtb virulence. These findings may be leveraged for drug discovery and will be of broad interest to those studying bacterial pathogenesis.

Reviewed by eLife, Rapid Reviews Infectious Diseases

Secreted small RNAs of Naegleria fowleri are biomarkers for diagnosis of primary amoebic meningoencephalitis

1. A. Cassiopeia Russell
2. Joseph Dainis
3. Jose Alexander
4. Ibne Karim M. Ali
5. Dennis E. Kyle

• Curated by eLife

  eLife Assessment

  This paper presents a method for detecting Naegleria fowleri infection, which is almost always fatal, using small RNA from blood. This could be an important advance since early detection might improve treatment outcomes. The mouse work is methodologically solid, but only a very small number of human samples were available for human validation.

Reviewed by eLife

In vitro and in vivo synergy of Vancomycin and β-lactams against drug-resistant Mycobacterium tuberculosis and Non-tuberculous mycobacteria

1. Alok Kumar Singh
2. Pradip Malik
3. Atri Mukhopadhyay
4. Mohmmad Imran
5. Mohammad Naiyaz Ahmad
6. Juned Ali
7. Sangita Pramanik
8. Reetu Maindolia
9. Aditi Ghosh
10. Pratiksha Karaulia
11. Umesh D Gupta
12. Kalyan Mitra
13. Sidharth Chopra
14. Arunava Dasgupta

Reviewed by PREreview

Closest relatives of poxviruses are spread in the gut of humans and animals worldwide: the egoviruses

1. Morgan Gaïa
2. Hans-Joachim Ruscheweyh
3. A. Murat Eren
4. Eugene V. Koonin
5. Shinichi Sunagawa
6. Mart Krupovic
7. Tom O. Delmont

Reviewed by Arcadia Science

Complex system modeling reveals oxalate homeostasis is driven by diverse oxalate-degrading bacteria

1. Sromona D Mukherjee
2. Carlos Batagello
3. Ava Adler
4. Jose Agudelo
5. Anna Zampini
6. Mangesh Suryavanshi
7. Andrew Nguyen
8. Terry Orr
9. Denise Dearing
10. Manoj Monga
11. Aaron W Miller

• Curated by eLife

  eLife Assessment

  This work presents a valuable approach based on a complex systems theoretical framework to characterize diet-host-microbe interactions and develop targeted bacteriotherapies through a three-phase workflow. Despite the partial support of the description and experimental setup of the 'complex systems theoretical approach,' the collected data are solid and advance our understanding of oxalate bacterial metabolism in microbial communities. This study will interest researchers working on gut microbiomes and the possible modulation of host-microbial interactions.

Reviewed by eLife

Wag31, a membrane tether, is crucial for lipid homeostasis in mycobacteria

1. Yogita Kapoor
2. Himani Khurana
3. Debatri Dutta
4. Arnab Chakraborty
5. Anshu Priya
6. Archana Singh
7. Siddhesh S Kamat
8. Neeraj Dhar
9. Thomas John Pucadyil
10. Vinay Kumar Nandicoori

• Curated by eLife

  eLife Assessment

  Understanding bacterial growth mechanisms potentially uncover novel drug targets which are crucial for maintaining cellular viability, particularly for bacterial pathogens. In this important study, Kapoor et al, investigate the role of Wag31 in lipid and peptidoglycan biosynthesis in mycobacteria. A detailed analysis of Wag31 domain architecture revealed a role in membrane tethering. More specifically, the N-terminal and C-terminal domains appeared to have distinct functional roles. The data presented are solid and support the conclusion made. This study will be of broad interest to microbiologists and molecular biologists.

Reviewed by eLife

Enhanced virulence and stress tolerance are signatures of epidemiologically successful Shigella sonnei

1. Sydney L Miles
2. Dilys Santillo
3. Vincenzo Torraca
4. Ana Teresa López Jiménez
5. Claire Jenkins
6. Stephen Baker
7. Kate S Baker
8. Vanessa Sancho-Shimizu
9. Kathryn E Holt
10. Serge Mostowy

Reviewed by PREreview