Microbiology

  2. Evaluation of antibiotic and peptide vaccine strategies for mirror bacterial infections

    1. Alexander Kleinman
    2. Joe Torres
    3. Brian Wang

    • Curated by eLife

      eLife Assessment

      This important study experimentally probes potential antibiotic activity against hypothetical "mirror bacteria" with reversed chirality, showing that D-enantiomers of several approved antibiotics largely lack activity against natural bacteria (as a proxy for mirror organisms) and that conjugated D-peptides can elicit strong binding antibody responses in mice when adjuvanted. The evidence is solid for these core observations but incomplete on issues of chiral purity, functional antibody assays, replicates, and pharmacodynamic readouts; the work also overreaches in extrapolations without deeper mechanistic integration or native-format validation. Overall, the work offers a cautious, relevant contribution to mirror microbiology discussions and will interest infectious disease researchers.

    Reviewed by eLife

    4 evaluationsAppears in 1 listLatest version Latest activity

  3. Role of zinc in growth, stress response and virulence gene expression of pathogenic Mucorale Rhizopus arrhizus

    1. Rachna Singh
    2. Anjna Kumari
    3. Pavneet Kaur
    4. Jasdeep Kaur

    Reviewed by Rapid Reviews Infectious Diseases

    1 evaluationAppears in 1 listLatest version Latest activity

  5. Chemostat culturing reduces fecal eukaryotic virus load and delays diarrhea after virome transplantation

    1. Simone Margaard Offersen
    2. Signe Adamberg
    3. Malene Roed Spiegelhauer
    4. Xiaotian Mao
    5. Torben Sølbeck Rasmussen
    6. Frej Larsen
    7. Jingren Zhong
    8. Duc Ninh Nguyen
    9. Dennis Sandris Nielsen
    10. Lise Aunsholt
    11. Thomas Thymann
    12. Kaarel Adamberg
    13. Anders Brunse

    • Curated by eLife

      eLife Assessment

      In this valuable study, the authors report on an innovative chemostat propagation system to reduce eukaryotic viruses while retaining phages in mixtures used for FVTs (fecal virome transplant). The authors hypothesized that chemostat-propagated viromes could modulate the gut microbiota and reduce necrotizing enterocolitis (NEC) lesions while avoiding potential side effects, such as earlier onset of diarrhea. Although no effect on NEC could be demonstrated, the revised document addressed the other concerns and is much improved from its original version. The study is convincing in that it integrates in vitro fermentation, high-resolution metagenomics, immunogenicity assays, and in vivo validation, demonstrating the potential of FVT using eukaryotic-free virome-based therapeutics.

    Reviewed by eLife

    9 evaluationsAppears in 1 listLatest version Latest activity

  6. Fine-tuning of outer membrane–peptidoglycan tethering by the redox-active lipoprotein LppB from Salmonella enterica

    1. Elisa S Pierre Despas
    2. Seung-Hyun Cho
    3. Bogdan I Iorga
    4. Jean-François Collet

    • Curated by eLife

      eLife Assessment

      This important study convincingly demonstrates how bacterial cells can modulate outer membrane-peptidoglycan tethering by expressing two different Lpp homologs with distinct cross-linking efficiencies, revealing that Salmonella typhimurium LppB forms disulfide-based homodimers (or heterotrimers with Lpp when present) and is covalently attached to peptidoglycan primarily via the L,D-transpeptidase LdtB at residue K58. The evidence supporting the authors' claims is solid, including the regulatory role of LppB dimerization for its abundance in E. coli and its ability to inhibit Lpp/A crosslinking to peptidoglycan, although additional analysis and quantification of muropeptides in wild-type E. coli overexpressing LppB would further strengthen the findings. Overall, the work will be of great interest to microbiologists studying cell envelope biogenesis.

    Reviewed by eLife

    5 evaluationsAppears in 1 listLatest version Latest activity

  7. β-Coronaviruses use lysosomal organelles for cellular egress

    1. S Ghosh
    2. TA Dellibovi-Ragheb
    3. E Pak
    4. Q Qiu
    5. M Fisher
    6. PM Takvorian
    7. C Bleck
    8. V Hsu
    9. AR Fehr
    10. S Perlman
    11. SR Achar
    12. MR Straus
    13. GR Whittaker
    14. CAM de Haan
    15. G Altan-Bonnet
    16. N Altan-Bonnet

    Reviewed by Rapid Reviews Infectious Diseases, ScreenIT

    4 evaluationsAppears in 2 listsLatest version Latest activity

  8. The riboflavin biosynthetic pathway as a novel target for antifungal drugs against Candida species

    1. Jana Nysten
    2. Arne Peetermans
    3. Dries Vaneynde
    4. Stef Jacobs
    5. Liesbeth Demuyser
    6. Patrick Van Dijck

    Reviewed by Rapid Reviews Infectious Diseases

    3 evaluationsAppears in 1 listLatest version Latest activity

  9. Immunoreactive peptide maps of SARS-CoV-2

    1. Nischay Mishra
    2. Xi Huang
    3. Shreyas Joshi
    4. Cheng Guo
    5. James Ng
    6. Riddhi Thakkar
    7. Yongjian Wu
    8. Xin Dong
    9. Qianlin Li
    10. Richard S. Pinapati
    11. Eric Sullivan
    12. Adrian Caciula
    13. Rafal Tokarz
    14. Thomas Briese
    15. Jiahai Lu
    16. W. Ian Lipkin

    Reviewed by Rapid Reviews Infectious Diseases, ScreenIT

    4 evaluationsAppears in 2 listsLatest version Latest activity

  10. Post-sampling degradation of viral RNA in wastewater impacts the quality of PCR-based concentration estimates

    1. James D. Munday
    2. Jolinda de Korne-Elenbaas
    3. Charles Gan
    4. Adrian Lison
    5. Julien Riou
    6. Christoph Ort
    7. Timothy R. Julian
    8. Tanja Stadler

    Reviewed by Rapid Reviews Infectious Diseases

    5 evaluationsAppears in 1 listLatest version Latest activity
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