Microbiology

Unraveling CRP/cAMP-mediated metabolic regulation in Escherichia coli persister cells

1. Han G Ngo
2. Sayed Golam Mohiuddin
3. Aina Ananda
4. Mehmet Orman

• Curated by eLife

  eLife Assessment

  The study reports an important finding on the role of the global metabolic regulator Crp/cAMP in the formation of antibiotic persister Escherichia coli. The evidence supporting the claims is solid including metabolomic analysis and characterization of many mutant strains.

Reviewed by eLife

Heterogeneous efflux pump expression underpins phenotypic resistance to antimicrobial peptides

1. Ka Kiu Lee
2. Urszula Łapińska
3. Giulia Tolle
4. Maureen Micaletto
5. Bing Zhang
6. Wanida Phetsang
7. Anthony D Verderosa
8. Brandon M Invergo
9. Joseph Westley
10. Attila Bebes
11. Raif Yuecel
12. Paul A O'Neill
13. Audrey Farbos
14. Aaron R Jeffries
15. Stineke van Houte
16. Pierluigi Caboni
17. Mark AT Blaskovich
18. Benjamin E Housden
19. Krasimira Tsaneva-Atanasova
20. Stefano Pagliara

• Curated by eLife

  eLife Assessment

  This important study by Lee et al. explores the heterogeneous response of non-growing bacteria to the antimicrobial peptide (AMP) tachyplesin. The authors identify a subpopulation of cells that evade lethal damage by limiting the intracellular accumulation of a fluorescently labeled tachyplesin analog. The study provides compelling evidence that reduced drug accumulation underlies the decreased susceptibility of this subpopulation to the AMP. The molecular basis of this phenotype is well supported by the data.

Reviewed by eLife

Deletion of the moeA gene in Flavobacterium IR1 drives structural color shift from green to blue and alters polysaccharide metabolism

1. Álvaro Escobar Doncel
2. Constantinos Patinios
3. Alexandre Campos
4. Maria Beatriz Walter Costa
5. Maria V Turkina
6. Maria Murace
7. Raymond HJ Staals
8. Silvia Vignolini
9. Bas E Dutilh
10. Colin J Ingham

• Curated by eLife

  eLife Assessment

  This manuscript presents important findings on how structural color can be manipulated through a specific single-gene mutation in a motile bacterium. Compelling data provide a promising model to identify genes and molecular mechanisms supporting this widespread optical phenomenon. This work will be of interest to biophysicists and microbiologists working on structural colors and Flavobacterium.

Reviewed by eLife

Flexibility and modulation of translation initiation in enterovirus genomes

1. Rhian L. O’Connor
2. Georgia M. Cook
3. Jacqueline Hankinson
4. Ksenia Fominykh
5. Samantha H. Cheng
6. Daniel A. Nash
7. Aurélie Cenier
8. Komal M. Nayak
9. Stephen C. Graham
10. Janet E. Deane
11. Matthias Zilbauer
12. Andrew E. Firth
13. Valeria Lulla

Reviewed by Review Commons

Effect of Azithromycin treatment on the microbial composition, functional dynamics and resistomes of endocervical, vaginal and rectal microbiomes of women in Fiji with Chlamydia trachomatis infection

1. Sankhya Bommana
2. Olusola Olagoke
3. Yi-Juan Hu
4. Roselle Wang
5. Mike Kama
6. Morgan Dehdashti
7. Reshma Kodimerla
8. Timothy D. Read
9. Deborah Dean

Reviewed by Rapid Reviews Infectious Diseases

The microbiome interacts with the circadian clock and dietary composition to regulate metabolite cycling in the Drosophila gut

1. Yueliang Zhang
2. Sara B Noya
3. Yongjun Li
4. Jichao Fang
5. Amita Sehgal

• Curated by eLife

  eLife Assessment

  This study presents valuable findings about daily rhythm changes of the Drosophila melanogaster adult gut metabolome, which is shown to be dependent on the circadian clock genotype, dietary regime and composition, and gut microbiota. The phenomena observed are supported by convincing experimental evidence. The general descriptive approach limits the power of the proposed conclusions. The work will be of interest to a broad range of physiology specialists

Reviewed by eLife

Common biological features of Mycobacterium tuberculosis MmpL3 inhibitors

1. Lauren Ames
2. Renee Allen
3. Helena I. Boshoff
4. Laura Cleghorn
5. Curtis Engelhart
6. Dirk Schnappinger
7. Tanya Parish

Reviewed by Rapid Reviews Infectious Diseases

Myosin light chain 3 serves as a receptor for nervous necrosis virus entry into host cells via the macropinocytosis pathway

1. Lan Yao
2. Wanwan Zhang
3. Xiaogang Yang
4. Meisheng Yi
5. Kuntong Jia

• Curated by eLife

  eLife Assessment

  The findings in this manuscript are fundamental because they identify an entry receptor MYL3 that belongs to the myosin family as a possible target that could inhibit a virus that has a high impact on aquaculture. The evidence is convincing as it contains strong in vitro and in vivo data that support their conclusions; however, studies on the presence of MYL3 in NNV target tissues will further strengthen their claims

Reviewed by eLife

Transcriptional antitermination integrates the expression of loci of diverse phage origin in the chimeric Bartonella Gene Transfer Agent BaGTA

1. Aleksandr Korotaev
2. Quirin Niggli
3. Valeria Congedi
4. Christoph Dehio

• Curated by eLife

  eLife Assessment

  This manuscript describes an important finding of the transcriptional control of a chimeric gene transfer agents (GTA) cluster in Bartonella by a processive anti-termination factor (BrrG). The evidence provided is convincing. This manuscript will interest researchers working on transcriptional regulation, horizontal gene transfer, and phages.

Reviewed by eLife