Microbiology

Zika virus remodels and hijacks IGF2BP2 ribonucleoprotein complex to promote viral replication organelle biogenesis

1. Clément Mazeaud
2. Stefan Pfister
3. Jonathan E Owen
4. Higor Sette Pereira
5. Flavie Charbonneau
6. Zachary E Robinson
7. Anaïs Anton
8. Cheyanne L Bemis
9. Aïssatou Aïcha Sow
10. Trushar R Patel
11. Christopher J Neufeldt
12. Pietro Scaturro
13. Laurent Chatel-Chaix

• Curated by eLife

  eLife assessment

  This valuable study combines multidisciplinary approaches to examine the role of insulin-like growth factor 2 mRNA-binding protein 2 (IGF2BP2) as a potential novel host dependency factor for Zika virus. The main claims are supported by the data but remain incomplete. The evidence would be strengthened by improving the western blot analyses and adjusting the toning of their claims in relation to the role of IGF2BP2 for viral replication. With the experimental evidence strengthened, this work will be of interest to virologists working on flaviviruses.

Reviewed by eLife

β-1,6-glucan plays a central role in the structure and remodeling of the bilaminate fungal cell wall

1. Clara Bekirian
2. Isabel Valsecchi
3. Sophie Bachellier-Bassi
4. Cyril Scandola
5. J. Iñaki Guijarro
6. Murielle Chauvel
7. Thierry Mourer
8. Neil A.R. Gow
9. Vishukumar Aimanianda
10. Christophe d’Enfert
11. Thierry Fontaine

• Curated by eLife

  eLife assessment

  The paper will be of broad interest to fungal biologists and fungal immunologists seeking to understand the biosynthesis of the fungal cell wall, in particular of ß-1,6-glucan synthesis and the importance of this so far understudied constituent of the cell wall for cell wall integrity and immune response. The study is of fundamental significance and adds structural clarity to the presence, genetic, and biochemical basis of this difficult-to-analyze carbohydrate. It opens the potential for understanding its role in immune recognition and potentially as a drug target. Overall, the data is compelling, properly controlled and analyzed, but a few aspects need to be reconsidered.

Reviewed by eLife

Persistent cross-species transmission systems dominate Shiga toxin-producing Escherichia coli O157:H7 epidemiology in a high incidence region: a genomic epidemiology study

1. Gillian AM Tarr
2. Linda Chui
3. Kim Stanford
4. Emmanuel W Bumunang
5. Rahat Zaheer
6. Vincent Li
7. Stephen B Freedman
8. Chad R Laing
9. Tim A McAllister

• Curated by eLife

  eLife assessment

  This work revealed numerous distinct lineages that evolved within a local human population in Alberta, Canada, leading to persistent cases of E. coli O157:H7 infections for over a decade and highlighting the ongoing involvement of local cattle in disease transmission, as well as the possibility of intermediate hosts and environmental reservoirs. This is a useful study that also showed a shift towards more virulent stx2a-only strains becoming predominant in the local lineages. The paper's evidence supporting the role played by cattle in the transmission system of E. coli O157:H7 in Alberta is currently incomplete, based on potential sampling issues associated with the selection of isolates as raised by the reviewers.

Reviewed by eLife

Drug-induced differential culturability in diverse strains of Mycobacterium tuberculosis

1. Valerie F. A. March
2. Nino Maghradze
3. Kakha Mchedlishvili
4. Teona Avaliani
5. Rusudan Aspindzelashvili
6. Zaza Avaliani
7. Maia Kipiani
8. Nestani Tukvadze
9. Levan Jugheli
10. Selim Bouaouina
11. Anna Doetsch
12. Galo A. Goig
13. Sebastien Gagneux
14. Sonia Borrell

Reviewed by PREreview

Evolution of Omicron lineage towards increased fitness in the upper respiratory tract in the absence of severe lung pathology

1. Arthur Wickenhagen
2. Meaghan Flagg
3. Julia Port
4. Claude Kwe Yinda
5. Kerry Goldin
6. Shane Gallogly
7. Jonathan E Schulz
8. Tessa Lutterman
9. Brandi Williamson
10. Franziska Kaiser
11. Reshma Mukesh
12. Sarah Van Tol
13. Brian Smith
14. Neeltje van Doremalen
15. Colin Russell
16. Emmie de Wit
17. Vincent Munster

Reviewed by Rapid Reviews Infectious Diseases

Telomerase RNA component knockout exacerbates S. aureus pneumonia by extensive inflammation and dysfunction of T cells

1. Yasmina Reisser
2. Franziska Hornung
3. Antje Häder
4. Thurid Lauf
5. Sandor Nietzsche
6. Bettina Löffler
7. Stefanie Deinhardt-Emmer

• Curated by eLife

  eLife assessment

  This is a very interesting study that links inflammatory reactivity and T-cell immunity in pathologies associated with pneumonia in the context of the aging process (telomerase functionality). The authors have relied on results from experiments using a mouse model (Terc-deletion), that is used in studies on aging. The questions are relevant, the methodology is appropriate, and the results represent a set of useful findings. However, on the whole, the evidence is not very strong owing to the low power of the study, some flaws in experimental design, lack of rigorous controls, and inadequate approaches to analyzing immune function, thus making the study incomplete in support of its claims.

Reviewed by eLife

Akkermansia muciniphila identified as key strain to alleviate gut barrier injury through Wnt signaling pathway

1. Xin Ma
2. Li Meng
3. Yuanyuan Zhang
4. Tingting Xu
5. Xinchen Zhou
6. Mengqi Qian
7. Zhiren Yang
8. Xinyan Han

• Curated by eLife

  eLife assessment

  The work by Han and collaborators describes valuable findings on the role of Akkermansia muciniphila during ETEC infection. If confirmed, these findings will add to a growing list of beneficial properties of this organism. Although the strength of the evidence used to justify the conclusions in the manuscript is solid, the issues raised about the sequencing method used should be addressed.

Reviewed by eLife

Spatial integration of sensory input and motor output in Pseudomonas aeruginosa chemotaxis through colocalized distribution

1. Zhengyu Wu
2. Maojin Tian
3. Sanyuan Fu
4. Min Chen
5. Rongjing Zhang
6. Junhua Yuan

• Curated by eLife

  eLife assessment

  This valuable study by Wu et al presents data on bacterial cell organization, with the goal of demonstrating that the two structures that account for bacterial motility - the chemotaxis complex and the flagella - colocalize to the same pole in Pseudomonas aeruginosa cells and expose the regulation underlying their spatial organization and functioning. The subject is of importance and the manuscript is well written. However, the work is incomplete and the conclusions are too strong for the presented data. This manuscript will be of interest to cell biologists, mainly those studying bacteria, but not only, if the evidence is substantiated, the assumptions clarified, and the novelty is made clear during the revision process.

Reviewed by eLife

The bat Influenza A virus subtype H18N11 induces nanoscale MHCII clustering upon host cell attachment

1. Maria Kaukab Osman
2. Jonathan Robert
3. Lukas Broich
4. Dennis Frank
5. Robert Grosse
6. Martin Schwemmle
7. Antoni Wrobel
8. Kevin Ciminski
9. Christian Sieben
10. Peter Reuther

Reviewed by preLights

The RND efflux pump EefABC is highly conserved within lineages of E. coli commonly associated with infection

1. Hannah L. Pugh
2. Elizabeth M. Darby
3. Leah Burgess
4. Abigail L. Colclough
5. Asti-Rochelle Meosa John
6. Steven Dunn
7. Christopher Connor
8. Eoughin A. Perry
9. Alan McNally
10. Vassiliy N. Bavro
11. Jessica M. A. Blair

Reviewed by PREreview