Microbiology

Engineering probiotic Escherichia coli Nissle 1917 to block transfer of multiple antibiotic resistance genes by exploiting a type I CRISPR-Cas system

1. Mengdie Fang
2. Ruiting Zhang
3. Chenyu Wang
4. Zhizhi Liu
5. Mingyue Fei
6. Biao Tang
7. Hua Yang
8. Dongchang Sun

Reviewed by Rapid Reviews Infectious Diseases

Comparative analysis of culture- and ddPCR-based wastewater surveillance for carbapenem-resistant bacteria

1. Siyi Zhou
2. Esther G. Lou
3. Julia Schedler
4. Katherine B. Ensor
5. Loren Hopkins
6. Lauren B. Stadler

Reviewed by PREreview

The role of MICOS in organizing mitochondrial cristae in malaria parasites

1. Silvia Tassan-Lugrezin
2. Irina Bregy
3. Judith López Orra
4. Nicholas I Proellochs
5. Geert-Jan van Gemert
6. Rianne Stoter
7. Felix Evers
8. Taco WA Kooij
9. Laura van Niftrik

• Curated by eLife

  eLife Assessment

  This study provides valuable insights into mitochondrial cristae organization in Plasmodium falciparum, particularly in the context of its divergent MICOS composition. The authors present convincing evidence, supported by phenotypic and morphological analyses, that cristae junction maintenance can be uncoupled from de novo cristae formation, reinforcing an emerging model of mitochondrial inner membrane organization. Notably, the absence of Mic10 alongside an enlarged and divergent MICOS complex highlights an intriguing evolutionary adaptation, although further characterization of the complex would strengthen the study's overall significance.

  [Editors' note: this paper was reviewed by Review Commons.]

Reviewed by eLife, Review Commons

Identifying a novel mechanism of L-leucine uptake in Mycobacterium tuberculosis using a chemical genomic approach

1. Nisheeth Agarwal
2. Himanshu Gogoi
3. Eeba
4. Linus Augustin
5. Md. Younus Khan
6. Yashwant Kumar
7. Sayan Kumar Bhowmick
8. Bappaditya Dey

• Curated by eLife

  eLife Assessment

  By screening an FDA-approved small-molecule library against a leucine-dependent M. tuberculosis strain, this study identifies semapimod as an inhibitor of Mtb growth that functions by impairing leucine import. The work is useful in linking leucine uptake to cell wall lipid biology in Mtb. However, the mechanistic understanding remains incomplete. Additional experimental evidence is required to clarify how PDIM contributes to or regulates leucine uptake.

Reviewed by eLife

TITAN-RNA: A hybrid-capture sequencing panel detects known and unknown Flaviviridae for diagnostics and vector surveillance

1. Chloe M. Murrell
2. Aiman Sabaawy
3. Xiyu Wang
4. Nagi Hashimoto
5. Kristina Ceres
6. Yining Sun
7. Jordan D. Zehr
8. Aine Lehane
9. Emily Mader
10. Natalie Bailey
11. Marie V. Lilly
12. Laura Plimpton
13. Guillaume Reboul
14. Joel J. Brown
15. Kelly Sams
16. Lauren Singh
17. Ethan Seiz
18. Ellie Bourgikos
19. Chantal B.F. Vogels
20. Alexander T. Ciota
21. Victoria Schnurr
22. Jennifer Grenier
23. Xavier Berthet
24. Ana I. Bento
25. Laura Harrington
26. Maria Diuk-Wasser
27. Ximena Olarte-Castillo
28. Laura B. Goodman

Reviewed by PREreview

Evolution of Omicron lineage towards increased fitness in the upper respiratory tract in the absence of severe lung pathology

1. Arthur Wickenhagen
2. Meaghan Flagg
3. Julia R. Port
4. Claude Kwe Yinda
5. Kerry Goldin
6. Shane Gallogly
7. Jonathan E. Schulz
8. Tessa Lutterman
9. Brandi N. Williamson
10. Franziska Kaiser
11. Reshma K. Mukesh
12. Sarah van Tol
13. Brian Smith
14. Neeltje van Doremalen
15. Colin A. Russell
16. Emmie de Wit
17. Vincent J. Munster

Reviewed by Rapid Reviews Infectious Diseases

Flow cytometry-based isolation of Salmonella -containing phagosomes combined with ultra-sensitive proteomics reveals novel insights into host-pathogen interactions

1. Ritika Chatterjee
2. José Luis Marin Rubio
3. Francesca Romana Cianfanelli
4. Andrew Frey
5. Benjamin Bernard Armando Raymond
6. Abeer Dannoura
7. Camila Valenzuela
8. Meihan Meng
9. Tiaan Heunis
10. Mengchun Li
11. Frances Sidgwick
12. Jost Enninga
13. Andrew Filby
14. Matthias Trost

Reviewed by Review Commons