Microbiology

Flow cytometry-based isolation of Salmonella -containing phagosomes combined with ultra-sensitive proteomics reveals novel insights into host-pathogen interactions

1. Ritika Chatterjee
2. José Luis Marin Rubio
3. Francesca Romana Cianfanelli
4. Andrew Frey
5. Benjamin Bernard Armando Raymond
6. Abeer Dannoura
7. Camila Valenzuela
8. Meihan Meng
9. Tiaan Heunis
10. Mengchun Li
11. Frances Sidgwick
12. Jost Enninga
13. Andrew Filby
14. Matthias Trost

Reviewed by Review Commons

The cow udder is a potential coinfection site for influenza A viruses

1. Rute Maria Pinto
2. Colin P Sharp
3. Maia Beeson
4. Nunticha Pankaew
5. Jack A Hassard
6. Alexander Moxom
7. Callum Magill
8. Laura Tuck
9. Stephen Meek
10. Hui Min Lee
11. Kirsty Jensen
12. Inga Dry
13. Pedro Melo
14. Jiayun Yang
15. Wenfang Spring Tan
16. Ashwin Ashok Raut
17. Anamika Mishra
18. Sjaak de Wit
19. J Ross Fitzgerald
20. Jayne C Hope
21. Joanne Stevens
22. Tom Burdon
23. Kate Sutton
24. Cristina L Esteves
25. F Xavier Donadeu
26. Ian Brown
27. Wendy Barclay
28. Thomas P Peacock
29. Daniel H Goldhill
30. Munir Iqbal
31. Pablo R Murcia
32. Stuart M Haslam
33. Eleanor Gaunt
34. Paul Digard

• Curated by eLife

  eLife Assessment

  This study presents important findings that bovine mammary epithelial cells can be infected with both avian and human influenza A viruses, providing a potential site for viral reassortment. The evidence to support these claims is generally solid; however, the evidence suggesting lower permissiveness of cells from other organs is incomplete. The work will be of interest to virologists and evolutionary biologists working on cross-species transmission of viruses and pandemic preparedness.

Reviewed by eLife

Screening the MMV Pathogen Box reveals the mitochondrial bc1-complex as a drug target in mature Toxoplasma gondii bradyzoites

1. Deborah Maus
2. Elyzana Putrianti
3. Tobias Hoffmann
4. Michael Laue
5. Frank Seeber
6. Martin Blume

• Curated by eLife

  eLife Assessment

  This valuable study utilizes a newly developed approach to culture T gondii bradyzoites in myotubes, and then takes advantage of the antiparasitic compound collection known as the Pathogen Box, to find compounds that target both tachyzoite and bradyzoite forms of the parasite. A set of compounds yielding patterns consistent with targeting the mitochondrial bc1 complex was explored further, with convincing evidence for changes in ATP production in bradyzoites to support the conclusions about the importance of this complex. The paper will be interesting for parasitologists studying drug discovery of apicomplexan parasites.

Reviewed by eLife

Mitochondrial protein FgDML1 impacts DON toxin biosynthesis and cyazofamid sensitivity in Fusarium graminearum by affecting mitochondrial homeostasis

1. Chenguang Wang
2. Xuewei Mao
3. Weiwei Cong
4. Lin Yang
5. Yiping Hou

• Curated by eLife

  eLife Assessment

  This important study tackles an interesting aspect of fungal physiology: how a mitochondria-associated gene influences production of the secondary metabolite DON and fungicide sensitivity. The authors have improved the manuscript and the supporting evidence is convincing.

Reviewed by eLife

A Novel Rapid Host Cell Entry Pathway Determines Intracellular Fate of Staphylococcus aureus

1. Marcel Rühling
2. Fabio Schmelz
3. Kim Ulbrich
4. Fabian Schumacher
5. Julia Wolf
6. Maximilian Pfefferle
7. Magdalena Priester
8. Adriana Moldovan
9. Nadine Knoch
10. Andreas Iwanowitsch
11. Christian Kappe
12. Kerstin Paprotka
13. Burkhard Kleuser
14. Christoph Arenz
15. Martin J Fraunholz

• Curated by eLife

  eLife Assessment

  This valuable study proposes a novel rapid-entry mechanism for Staphylococcus aureus, involving the rapid release of calcium from lysosomes. The paper's strength lies in its very interesting hypothesis. The methods used are solid and adequately support the conclusions.

Reviewed by eLife

Out-of-balance Growth Enables Cost-free Synthesis of the Flagellum and Other Proteins in a Single Bacterium

1. Mayra Garcia-Alcala
2. Josiah C Kratz
3. Philippe Cluzel

• Curated by eLife

  eLife Assessment

  This important study addresses a discrepancy between population-level growth laws and single-cell correlations. It shows, for flagellar and synthetic genes in E. coli, that while gene expression of certain genes reduces population-average growth, expression levels positively correlate with growth at the single-cell level. The measurements are mostly convincing, and the proposed mechanism-inheritance of growth factors such as ribosomes during asymmetric division- explains this observation. The theoretical analysis would benefit from clearer explanations and robustness checks.

Reviewed by eLife

Quantitative RNA pseudouridine landscape reveals dynamic modification patterns and evolutionary conservation across bacterial species

1. Letong Xu
2. Shenghai Shen
3. Yizhou Zhang
4. Zhihao Guo
5. Beifang Lu
6. Jiadai Huang
7. Runsheng Li
8. Yitong Shen
9. Li-Sheng Zhang
10. Xin Deng

• Curated by eLife

  eLife Assessment

  This study illustrates a valuable application of BID-seq to bacterial RNA, allowing transcriptome-wide mapping of pseudouridine modifications across various bacterial species. The evidence presented includes solid data and analyses that would benefit from additional experimental validation. The work will interest a specialized audience involved in RNA biology.

Reviewed by eLife

Research advance: Unexpected plasticity in the life cycle of Trypanosoma brucei

1. Carina Praisler
2. Jaime N Lisack
3. Anna Sophie Kreis
4. Laura Hauf
5. Johanna Krenzer
6. Fabian Imdahl
7. Markus Engstler

• Curated by eLife

  eLife Assessment

  The manuscript concerns a fundamental and controversial question in Trypanosoma brucei biology and the parasite life cycle, providing further evidence that slender bloodstream forms can indeed infect Tsetse flies. The study is solid in design and execution, and addresses several criticisms made of the authors' earlier work. Nevertheless, some of the main conclusions are only partially supported: one issue is how, precisely, a "slender" bloodstream form is defined, and discrepancies with some results from other laboratories remain unexplained.

Reviewed by eLife

The phenotypic landscape of the mycobacterial cell

1. Nadia Herrera
2. Horia Todor
3. Lili M. Kim
4. Hannah N. Burkhart
5. Evan Billings
6. Allison Fay
7. Theodore C. Warner
8. So Young Lee
9. Natalie Y. Sayegh
10. Barbara Bosch
11. James Chen
12. Laura L. Kiessling
13. Michael S. Glickman
14. Filippo Mancia
15. Jeremy M. Rock
16. Carol A. Gross

Reviewed by Rapid Reviews Infectious Diseases

Ligand binding represses bacterial histidine kinase activity by inhibiting its dimerization

1. Gaurav D. Sankhe
2. Jiawei Xing
3. Merissa Xiao
4. John Buglino
5. Huilin Li
6. Igor B. Zhulin
7. Michael S. Glickman

Reviewed by Review Commons