Microbiology

Myosin light chain 3 serves as a receptor for nervous necrosis virus entry into host cells via the macropinocytosis pathway

1. Lan Yao
2. Wanwan Zhang
3. Xiaogang Yang
4. Meisheng Yi
5. Kuntong Jia

• Curated by eLife

  eLife Assessment

  The findings in this manuscript are fundamental because they identify an entry receptor MYL3 that belongs to the myosin family as a possible target that could inhibit a virus that has a high impact on aquaculture. The evidence is convincing as it contains strong in vitro and in vivo data that support their conclusions; however, studies on the presence of MYL3 in NNV target tissues will further strengthen their claims

Reviewed by eLife

Transcriptional antitermination integrates the expression of loci of diverse phage origin in the chimeric Bartonella Gene Transfer Agent BaGTA

1. Aleksandr Korotaev
2. Quirin Niggli
3. Valeria Congedi
4. Christoph Dehio

• Curated by eLife

  eLife Assessment

  This manuscript describes an important finding of the transcriptional control of a chimeric gene transfer agents (GTA) cluster in Bartonella by a processive anti-termination factor (BrrG). The evidence provided is convincing. This manuscript will interest researchers working on transcriptional regulation, horizontal gene transfer, and phages.

Reviewed by eLife

Bacteriophage infection drives loss of β-lactam resistance in methicillin-resistant Staphylococcus aureus

1. My Tran
2. Angel J Hernandez Viera
3. Patricia Q Tran
4. Erick Nilsen
5. Lily Tran
6. Charlie Y Mo

• Curated by eLife

  eLife Assessment

  The manuscript explores how bacterial evolution in the presence of lytic phages modulates b-lactams resistance and virulence properties in methicillin-resistant Staphylococcus aureus (MRSA). This important work improves our knowledge of how mutation in genes required for phage infection confers sensitivity to b-lactams and alter virulence properties. Altogether, the findings are convincing.

Reviewed by eLife

Fast Evolution of SOS-Independent Multi-Drug Resistance in Bacteria

1. Le Zhang
2. Yunpeng Guan
3. Yuen Yee Cheng
4. Nural N. Cokcetin
5. Amy L. Bottomley
6. Andrew Robinson
7. Elizabeth J. Harry
8. Antoine van Oijen
9. Qian Peter Su
10. Dayong Jin

• Curated by eLife

  eLife Assessment

  This study presents a valuable observation of how deletion of a major repair protein in bacteria can facilitate the rise of mutations that confer resistance against a range of different antibiotics. The data presented are convincing, and the authors addressed the concerns raised by the reviewers in their resubmission, improving the strength of their findings.

Reviewed by eLife

Identifying a novel mechanism of L-leucine uptake in Mycobacterium tuberculosis using a chemical genomic approach

1. Nisheeth Agarwal
2. Himanshu Gogoi
3. Eeba
4. Linus Augustin
5. Md. Younus Khan
6. Yashwant Kumar
7. Sayan Kumar Bhowmick
8. Bappaditya Dey

• Curated by eLife

  eLife Assessment

  By performing a chemical screen of an FDA-approved library of small molecules against a leucine-dependent Mtb strain, this work discovered that semapimod inhibits Mtb growth by impairing leucine import. The work is useful because it connects leucine uptake with the cell wall lipids in Mtb; however, it remains incomplete as the evidence supporting semapimod's ability to target leucine uptake needs more substantial proof. The work requires significant experimental evidence to identify leucine transporter(s) and determine how PDIM participates in leucine uptake.

Reviewed by eLife

Caffeic acid phenethyl ester protects Clostridioides difficile infection by toxin inhibition and microbiota modulation

1. Yan Guo
2. Yong Zhang
3. Guizhen Wang
4. Hongtao Liu
5. Jianfeng Wang
6. Xuming Deng
7. Liuqin He
8. Jiazhang Qiu

• Curated by eLife

  eLife Assessment

  This valuable study by Guo and colleagues reports the inhibitory activity of caffeic acid phenethyl ester (CAPE) against TcdB, a key toxin produced by Clostridioides difficile. C. difficile infections are a major public health concern, and this manuscript provides interesting data on toxin inhibition by CAPE, a potentially promising therapeutic alternative for this disease. The strength of the evidence to support the conclusions is solid, with some concerns about the moderate effects on the mouse infection model and direct binding assays of CAPE to the toxin.

Reviewed by eLife

Structure of a new capsid form and comparison with A-, B- and C-capsids clarify herpesvirus assembly

1. Alexander Stevens
2. Saarang Kashyap
3. Ethan Crofut
4. Ana Lucia Alverez-Cabrera
5. Jonathan Jih
6. Yun-Tao Liu
7. Z. Hong Zhou

Reviewed by Rapid Reviews Infectious Diseases

The hepatitis E virus capsid protein ORF2 counteracts cell-intrinsic antiviral responses to enable persistence in hepatocytes

1. Ann-Kathrin Mehnert
2. Sebastian Stegmaier
3. Carlos Ramirez
4. Vladimir Gonçalves Magalhães
5. Carla Siebenkotten
6. Jungen Hu
7. Ana Luisa Costa
8. Daniel Kirrmaier
9. Michael Knop
10. Xianfang Wu
11. Thibault Tubiana
12. Carl Herrmann
13. Marco Binder
14. Viet Loan Dao Thi

Reviewed by Review Commons

Influenza virus antagonizes self sensing by RIG-I to enhance viral replication

1. Mitchell P. Ledwith
2. Thomas Nipper
3. Kaitlin A. Davis
4. Deniz Uresin
5. Anastassia V. Komarova
6. Andrew Mehle

Reviewed by PREreview

A prophage-encoded anti-phage defense system that prevents phage DNA packaging by targeting the terminase complex

1. Gil Azulay
2. Nadejda Sigal
3. Keren Tzohar Rabinovich
4. Dan Koenigsfeld
5. Olga Stadnyuk
6. Avijit Das
7. Polina Lisitzin
8. Einat Biderman
9. Ilya Borovok
10. Anat A. Herskovits

Reviewed by preLights, PREreview