Microbiology

Quantification of sporozoite expelling by Anopheles mosquitoes infected with laboratory and naturally circulating P. falciparum gametocytes

1. Chiara Andolina
2. Wouter Graumans
3. Moussa Guelbeogo
4. Geert-Jan van Gemert
5. Jordache Ramijth
6. Soré Harouna
7. Zongo Soumanaba
8. Rianne Stoter
9. Marga Vegte-Bolmer
10. Martina Pangos
11. Photini Sinnis
12. Katharine Collins
13. Sarah G Staedke
14. Alfred B Tiono
15. Chris Drakeley
16. Kjerstin Lanke
17. Teun Bousema

• Curated by eLife

  eLife assessment

  This important study combines experimental infections with laboratory and field Plasmodium falciparum isolates to quantify the force of human malaria parasite transmission. By using compelling methodological approaches, the authors establish clear positive correlations between mosquito infection levels (as determined by oocyst numbers), sporozoite loads in salivary glands, and sporozoites expelled during feeding. The link between heterogenous infection levels in the mosquitoes and malaria transmission would be of interest to vector biologists, parasitologists, immunologists, and mathematical modellers.

Reviewed by eLife

A treasure trove of 1,034 actinomycete genomes

1. Tue Sparholt Jørgensen
2. Omkar Mohite
3. Eva B Sterndorff
4. Maria Alvarez-Arevalo
5. Kai Blin
6. Thomas J Booth
7. Pep Charusanti
8. David Faurdal
9. Troels Ø Hansen
10. Matin Nuhamunada
11. Anna-Sophie Mourched
12. Bernhard Ø Palsson
13. Tilmann Weber

Reviewed by Arcadia Science

Growing a Cystic Fibrosis-Relevant Polymicrobial Biofilm to Probe Community Phenotypes

1. Sarah Poirier
2. Fabrice Jean-Pierre

Reviewed by Arcadia Science

Pinpointing the microbiota of tardigrades: What is really there?

1. Bartłomiej Surmacz
2. Daniel Stec
3. Monika Prus‐Frankowska
4. Mateusz Buczek
5. Łukasz Michalczyk
6. Piotr Łukasik

Reviewed by Arcadia Science

The Toxoplasma monocarboxylate transporters are involved in the metabolism within the apicoplast and are linked to parasite survival

1. Hui Dong
2. Jiong Yang
3. Kai He
4. Wen-Bin Zheng
5. De-Hua Lai
6. Jing Liu
7. Hui-Yong Ding
8. Rui-Bin Wu
9. Kevin M Brown
10. Geoff Hide
11. Zhao-Rong Lun
12. Xing-Quan Zhu
13. Shaojun Long

• Curated by eLife

  eLife assessment

  This study identifies two new transporters in the apicoplast, a non-photosynthetic organelle of apicomplexan parasites. While this is important work, it only partially reveals how essential these transporters are, as it does not address the metabolic function of the transporters for the parasite. Although the evidence is still incomplete, the results should be of interest to parasitologists and eukaryotic cell biologists.

Reviewed by eLife

Cryptic proteins translated from deletion-containing viral genomes dramatically expand the influenza virus proteome

1. Jordan N Ranum
2. Mitchell P Ledwith
3. Fadi G Alnaji
4. Meghan Diefenbacher
5. Richard Orton
6. Elizabeth Sloan
7. Melissa Güereca
8. Elizabeth M Feltman
9. Katherine Smollett
10. Ana da Silva Filipe
11. Michaela Conley
12. Alistair B Russell
13. Christopher B Brooke
14. Edward Hutchinson
15. Andrew Mehle

Reviewed by PREreview

Human pluripotent stem cell-derived hepatocyte-like cells for hepatitis D virus studies

1. Huanting Chi
2. Bingqian Qu
3. Angga Prawira
4. Lars Maurer
5. Jungen Hu
6. Rebecca M. Fu
7. Florian A. Lempp
8. Zhenfeng Zhang
9. Dirk Grimm
10. Xianfang Wu
11. Stephan Urban
12. Viet Loan Dao Thi

Reviewed by Review Commons

An assessment of Nano-RECall: Interpretation of Oxford Nanopore sequence data for HIV-1 drug resistance testing

1. Kayla Eileen Delaney
2. Trevor Ngobeni
3. Conan K. Woods
4. Carli Gordijn
5. Mathilda Claassen
6. Urvi Parikh
7. P. Richard Harrigan
8. Gert Uves van Zyl

Reviewed by PREreview

A dynamic bactofilin cytoskeleton cooperates with an M23 endopeptidase to control bacterial morphogenesis

1. Sebastian Pöhl
2. Manuel Osorio-Valeriano
3. Emöke Cserti
4. Jannik Harberding
5. Rogelio Hernandez-Tamayo
6. Jacob Biboy
7. Patrick Sobetzko
8. Waldemar Vollmer
9. Peter L Graumann
10. Martin Thanbichler

• Curated by eLife

  eLife assessment

  The manuscript explores the interplay between cytoskeletal bactofilins and cell wall hydrolases in bacterial morphogenesis, utilizing a range of methodologies from bacteriological to biochemical. The study provides important insights into bactofilin polymers' control over peptidoglycan synthesis and the identification of LdmC, supported by a comprehensive array of genetic, bioinformatic, biochemical, and biophysical tools. These convincing findings propose a conserved module governing bacterial morphogenesis, emphasizing the direct association of cell wall remodeling enzymes with a dynamic cytoskeleton, akin to mechanisms observed in other cellular processes such as cell growth and division.

Reviewed by eLife

Ebola virus sequesters IRF3 in viral inclusion bodies to evade host antiviral immunity

1. Lin Zhu
2. Jing Jin
3. Tingting Wang
4. Yong Hu
5. Hainan Liu
6. Ting Gao
7. Qincai Dong
8. Yanwen Jin
9. Ping Li
10. Zijing Liu
11. Yi Huang
12. Xuan Liu
13. Cheng Cao

• Curated by eLife

  eLife assessment

  This study explores how Ebola virus evades human immune responses. The study reports a potential new mechanism wherein Ebola virus traps human IRF3, a key transcription factor involved in immune signaling, into virus-produced "inclusion bodies". The topic is important, the paper has many merits, and the biochemical assays are solid. However, the current data do not clearly explain the relationship between the VP35 protein and IRF3.

Reviewed by eLife