Microbiology

NAD(H) homeostasis is essential for host protection mediated by glycolytic myeloid cells in tuberculosis

1. Hayden T. Pacl
2. Krishna C. Chinta
3. Vineel P. Reddy
4. Sajid Nadeem
5. Ritesh R. Sevalkar
6. Kierveshan Nargan
7. Kapongo Lumamba
8. Threnesan Naidoo
9. Joel N. Glasgow
10. Anupam Agarwal
11. Adrie J. C. Steyn

Reviewed by preLights

The commensal protist Tritrichomonas musculus exhibits a dynamic life cycle that induces extensive remodeling of the gut microbiota

1. Ana Popovic
2. Eric Yixiao Cao
3. Joanna Han
4. Nirvana Nursimulu
5. Eliza V.C. Alves-Ferreira
6. Kyle Burrows
7. Andrea Kennard
8. Noor Alsmadi
9. Michael E. Grigg
10. Arthur Mortha
11. John Parkinson

Reviewed by Arcadia Science

Structural basis for kinase inhibition in the tripartite E. coli HipBST toxin–antitoxin system

1. René L Bærentsen
2. Stine V Nielsen
3. Ragnhild B Skjerning
4. Jeppe Lyngsø
5. Francesco Bisiak
6. Jan Skov Pedersen
7. Kenn Gerdes
8. Michael A Sørensen
9. Ditlev E Brodersen

• Curated by eLife

  eLife assessment

  This important study presents an exhaustive structural analysis of a complete tripartite HipBST toxin-antitoxin system of the Enteropathogenic E. coli O127:H6, which represents a fascinating variation on the well-studied HipAB toxin-antitoxin system. The convincing data show that major features of the canonical HipAB system have been rerouted to form the tripartite HipBST, revealing a new mode of inhibition of a toxin kinase.

Reviewed by eLife

Antibiotic potentiation and inhibition of cross-resistance in pathogens associated with cystic fibrosis

1. Nikol Kadeřábková
2. R. Christopher D. Furniss
3. Evgenia Maslova
4. Kathryn E. Potter
5. Lara Eisaiankhongi
6. Patricia Bernal
7. Alain Filloux
8. Cristina Landeta
9. Diego Gonzalez
10. Ronan R. McCarthy
11. Despoina A.I. Mavridou

• Curated by eLife

  eLife assessment

  This important study demonstrates that disrupting a common protein-folding system restores sensitivity to antibiotics in drug-resistant clinical bacterial pathogens. Although some additional controls would be welcome, the work is overall convincing in showing that targeting protein folding can be used to combat multi-drug resistant pathogens, both by potentiating the efficacy of existing drugs and by therapeutic use of small-molecule inhibitors. This study is significant and timely as it furnishes a new strategy that is relevant to microbiologists and clinicians interested in combating antimicrobial resistance.

Reviewed by eLife

Structure of the two-component S-layer of the archaeon Sulfolobus acidocaldarius

1. Lavinia Gambelli
2. Mathew McLaren
3. Rebecca Conners
4. Kelly Sanders
5. Matthew C Gaines
6. Lewis Clark
7. Vicki AM Gold
8. Daniel Kattnig
9. Mateusz Sikora
10. Cyril Hanus
11. Michail N Isupov
12. Bertram Daum

• Curated by eLife

  eLife assessment

  This fundamental work substantially advances our structural understanding of S-layers in Archaea and how they are built to form formidable cell support structures able to stabilise the cytoplasmic membrane under harsh physicochemical conditions. The supporting evidence for the S-layer model is convincing, making excellent use of state-of-the-art 3D cryo-electron tomography reconstructions, although the proposed S-layer model would benefit from some additional validation.

Reviewed by eLife

Salmonella succinate utilisation is inhibited by multiple regulatory systems

1. Nicolas Wenner
2. Xiaojun Zhu
3. Will P. M. Rowe
4. Kristian Händler
5. Jay C. D. Hinton

Reviewed by Review Commons

Plasma activated water as a pre-treatment strategy in the context of biofilm-infected chronic wounds

1. Heema K.N. Vyas
2. Binbin Xia
3. David Alam
4. Nicholas P. Gracie
5. Joanna G. Rothwell
6. Scott A. Rice
7. Dee Carter
8. Patrick J. Cullen
9. Anne Mai-Prochnow

Reviewed by PREreview

Cell arrangement impacts metabolic activity and antibiotic tolerance in Pseudomonas aeruginosa biofilms

1. Hannah Dayton
2. Julie Kiss
3. Mian Wei
4. Shradha Chauhan
5. Emily LaMarre
6. William Cole Cornell
7. Chase J. Morgan
8. Anuradha Janakiraman
9. Wei Min
10. Raju Tomer
11. Alexa Price-Whelan
12. Jasmine A Nirody
13. Lars E.P. Dietrich

Reviewed by PREreview

SIRT-1 is required for release of enveloped enteroviruses

1. Alagie Jassey
2. James Logue
3. Stuart Weston
4. Michael A Wagner
5. Ganna Galitska
6. Katelyn Miller
7. Matthew Frieman
8. William T Jackson

• Curated by eLife

  eLife assessment

  The presence or absence of a surrounding envelope, previously a clear distinguishing feature of different viruses, has been blurred by the recent recognition that many so-called 'nonenveloped' viruses are released from cells as quasi-enveloped virions cloaked in host cell membranes. This mechanism of viral egress allows for non-lytic infection, and has potentially important implications for pathogenesis. In this manuscript, Jassey and colleagues provide solid evidence that the protein deacetylase SIRT-1 is required for the non-lytic release of enteroviruses in extracellular vesicles.

Reviewed by eLife

σ28-dependent small RNA regulation of flagella biosynthesis

1. Sahar Melamed
2. Aixia Zhang
3. Michal Jarnik
4. Joshua Mills
5. Aviezer Silverman
6. Hongen Zhang
7. Gisela Storz

• Curated by eLife

  eLife assessment

  This article provides important findings on how bacteria use small RNAs to regulate flagellar expression with implications for multiple fields. The data supporting the conclusions are convincing with a large amount of data that include results from phenotypic analyses, genomics approaches as well as in-vitro and in-vivo target identification and validation methods. This study on the varied effects of three sRNAs (UhpU, FliX and MotR) is of broad interest to RNA biochemists and microbiologists.

Reviewed by eLife