Microbiology

Commensal skin bacteria exacerbate inflammation and delay skin healing

1. Veda D. Khadka
2. Laura Markey
3. Magalie Boucher
4. Tami D. Lieberman

Reviewed by Arcadia Science

A modified BCG with depletion of enzymes associated with peptidoglycan amidation induces enhanced protection against tuberculosis in mice

1. Moagi Tube Shaku
2. Peter K Um
3. Karl L Ocius
4. Alexis J Apostolos
5. Marcos M Pires
6. William R Bishai
7. Bavesh D Kana

• Curated by eLife

  eLife assessment

  In this manuscript, the authors investigate whether the effects of the BCG vaccine on immunity to Mtb infection could be improved by inhibiting amidation of the peptidoglycan sidechains to allow for recognition by NOD-1. This is a very important area and an interesting new approach to improve vaccination for TB. The authors find that CRISPRi knockdown of murT-gatD causes rather dramatic cell wall defects, more accessible cell wall labeling, and results in attenuated growth in macrophages and mice. This forms a foundation for further study of whether an approach like that which is presented herein would improve vaccination responses in TB.

Reviewed by eLife

Restriction of Arginine Induces Antibiotic Tolerance in Staphylococcus aureus

1. Jeffrey A. Freiberg
2. Valeria M. Reyes Ruiz
3. Erin R. Green
4. Eric P. Skaar

Reviewed by PREreview, Rapid Reviews Infectious Diseases

Predicting phage-bacteria interactions at the strain level from genomes

1. Baptiste Gaborieau
2. Hugo Vaysset
3. Florian Tesson
4. Inès Charachon
5. Nicolas Dib
6. Juliette Bernier
7. Tanguy Dequidt
8. Héloïse Georjon
9. Olivier Clermont
10. Pascal Hersen
11. Laurent Debarbieux
12. Jean-Damien Ricard
13. Erick Denamur
14. Aude Bernheim

Reviewed by Arcadia Science

Cell-selective proteomics reveal novel effectors secreted by an obligate intracellular bacterial pathogen

1. Allen G. Sanderlin
2. Hannah Kurka Margolis
3. Abigail F. Meyer
4. Rebecca L. Lamason

Reviewed by Arcadia Science

Tick extracellular vesicles alter keratinocyte function in the skin epidermis

1. Liron Marnin
2. Luisa M. Valencia
3. Haikel N. Bogale
4. Hanna J. Laukaitis-Yousey
5. Agustin Rolandelli
6. Camila Rodrigues Ferraz
7. Anya J. O’Neal
8. Axel D. Schmitter-Sánchez
9. Emily Bencosme Cuevas
10. Thu-Thuy Nguyen
11. Brenda Leal-Galvan
12. David M. Rickert
13. M. Tays Mendes
14. Sourabh Samaddar
15. L. Rainer Butler
16. Nisha Singh
17. Francy E. Cabrera Paz
18. Alejandra Wu-Chuang
19. Jonathan D. Oliver
20. Julie M Jameson
21. Ulrike G. Munderloh
22. Adela S. Oliva Chávez
23. Albert Mulenga
24. Sangbum Park
25. David Serre
26. Joao H.F. Pedra

Reviewed by Arcadia Science

Host and viral determinants of airborne transmission of SARS-CoV-2 in the Syrian hamster

1. Julia R Port
2. Dylan H Morris
3. Jade C Riopelle
4. Claude Kwe Yinda
5. Victoria A Avanzato
6. Myndi G Holbrook
7. Trenton Bushmaker
8. Jonathan E Schulz
9. Taylor A Saturday
10. Kent Barbian
11. Colin A Russell
12. Rose Perry-Gottschalk
13. Carl Shaia
14. Craig Martens
15. James O Lloyd-Smith
16. Robert J Fischer
17. Vincent J Munster

• Curated by eLife

  eLife assessment

  This manuscript describes rigorous experiments that provide a wealth of virologic, respiratory physiology, and particle aerodynamic data pertaining to aerosol transmission of SARS-CoV-2 between infected Syrian hamsters. The significance of the paper is fundamental because infection is compared between alpha and delta variants, and because viral load is assessed via numerous assays (gRNA, sgRNA, TCID) and in tissues as well as the ambient environment of the cage. The strength of evidence is compelling.

Reviewed by eLife

Genome-wide Functional Characterization of Escherichia coli Promoters and Sequence Elements Encoding Their Regulation

1. Guillaume Urtecho
2. Kimberly D. Insigne
3. Arielle D. Tripp
4. Marcia S. Brinck
5. Nathan B. Lubock
6. Christopher Acree
7. Hwangbeom Kim
8. Tracey Chan
9. Sriram Kosuri

• Curated by eLife

  eLife assessment

  Urtecho et al. use genome-integrated massively parallel reporter assays to catalog and characterize promoters throughout the Escherichia coli genome. The result is a state-of-the-art atlas of promoters, coupled with information on their regulation, that is readily accessible through the website http://ecolipromoterdb.com. This compelling work provides an important resource for researchers studying bacterial transcriptional regulation.

Reviewed by eLife

Refinement of the “ Candidatus Accumulibacter” Genus Based on a Metagenomic Analysis of Biological Nutrient Removal (BNR) Pilot-Scale Plants Operated with Reduced Aeration

1. Rachel D. Stewart
2. Kevin S. Myers
3. Carly Amstadt
4. Matt Seib
5. Katherine D. McMahon
6. Daniel R. Noguera

Reviewed by Arcadia Science

Sterile protection against Plasmodium vivax malaria by repeated blood stage infection in a non-human primate model

1. Nicanor Obaldía
2. Joao Luiz Da Silva Filho
3. Marlon Núñez
4. Katherine A. Glass
5. Tate Oulton
6. Fiona Achcar
7. Grennady Wirjanata
8. Manoj Duraisingh
9. Philip Felgner
10. Kevin K.A. Tetteh
11. Zbynek Bozdech
12. Thomas D. Otto
13. Matthias Marti

Reviewed by Review Commons