Microbiology

SARS-CoV-2 Spike Affinity and Dynamics Exclude the Strict Requirement of an Intermediate Host

1. Matteo Castelli
2. Luigi Scietti
3. Nicola Clementi
4. Mattia Cavallaro
5. Silvia Faravelli
6. Alberta Pinnola
7. Elena Criscuolo
8. Roberta Antonia Diotti
9. Massimo Clementi
10. Federico Forneris
11. Nicasio Mancini

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Multiplexed detection of SARS-CoV-2 genomic and subgenomic RNA using in situ hybridization

1. Kofi K. Acheampong
2. Dylan L. Schaff
3. Benjamin L. Emert
4. Jonathan Lake
5. Sam Reffsin
6. Emily K. Shea
7. Courtney E. Comar
8. Leslie A. Litzky
9. Nigar A. Khurram
10. Rebecca L. Linn
11. Michael Feldman
12. Susan R. Weiss
13. Kathleen T. Montone
14. Sara Cherry
15. Sydney M. Shaffer

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VPS29 Exerts Opposing Effects on Endocytic Viral Entry

1. Daniel Poston
2. Yiska Weisblum
3. Alvaro Hobbs
4. Paul D. Bieniasz

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Spatial alanine metabolism determines local growth dynamics of Escherichia coli colonies

1. Francisco Díaz-Pascual
2. Martin Lempp
3. Kazuki Nosho
4. Hannah Jeckel
5. Jeanyoung K Jo
6. Konstantin Neuhaus
7. Raimo Hartmann
8. Eric Jelli
9. Mads Frederik Hansen
10. Alexa Price-Whelan
11. Lars EP Dietrich
12. Hannes Link
13. Knut Drescher

• Curated by eLife

  Evaluation Summary:

  In this manuscript the authors use a combination of transcriptomics, metabolomics, and quantitative measurements of growth to characterize the temporal and spatial distribution of cells with different metabolic states within colony of biofilms of the model bacterium Escherichia coli. They show that within the biofilm cells performing different metabolic functions are distributed in different regions of the colonies, and propose a model where nutrient cross-feeding through the amino acid alanine explains the phenotypic heterogeneity observed within the biofilm. The findings reported have potential broad implications for thinking about the spatial structure of communities of all bacterial species.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

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Age-Related Susceptibility of Ferrets to SARS-CoV-2 Infection

1. Mathias Martins
2. Maureen H. V. Fernandes
3. Lok R. Joshi
4. Diego G. Diel

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Identification of potent small molecule inhibitors of SARS-CoV-2 entry

1. Sonia Mediouni
2. Huihui Mou
3. Yuka Otsuka
4. Joseph Anthony Jablonski
5. Robert Scott Adcock
6. Lalit Batra
7. Dong-Hoon Chung
8. Christopher Rood
9. Ian Mitchelle S. de Vera
10. Ronald Rahaim Jr.
11. Sultan Ullah
12. Xuerong Yu
13. Yulia A. Getmanenko
14. Nicole M. Kennedy
15. Chao Wang
16. Tu-Trinh Nguyen
17. Mitchell Hull
18. Emily Chen
19. Thomas D. Bannister
20. Pierre Baillargeon
21. Louis Scampavia
22. Michael Farzan
23. Susana T. Valente
24. Timothy P. Spicer

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CPC-containing oral rinses inactivate SARS-CoV-2 variants and are active in the presence of human saliva

1. Enyia R. Anderson
2. Edward I. Patterson
3. Siobhan Richards
4. Ana K. Pitol
5. Thomas Edwards
6. Dominic Wooding
7. Kate Buist
8. Alison Green
9. Sayandip Mukherjee
10. Michael Hoptroff
11. Grant L. Hughes

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Rapid assessment of SARS-CoV-2–evolved variants using virus-like particles

1. Abdullah M. Syed
2. Taha Y. Taha
3. Takako Tabata
4. Irene P. Chen
5. Alison Ciling
6. Mir M. Khalid
7. Bharath Sreekumar
8. Pei-Yi Chen
9. Jennifer M. Hayashi
10. Katarzyna M. Soczek
11. Melanie Ott
12. Jennifer A. Doudna

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Limited Variation between SARS-CoV-2-Infected Individuals in Domain Specificity and Relative Potency of the Antibody Response against the Spike Glycoprotein

1. Hanora A. Van Ert
2. Dana W. Bohan
3. Kai Rogers
4. Mohammad Fili
5. Roberth A. Rojas Chávez
6. Enya Qing
7. Changze Han
8. Spencer Dempewolf
9. Guiping Hu
10. Nathan Schwery
11. Kristina Sevcik
12. Natalie Ruggio
13. Devlin Boyt
14. Michael A. Pentella
15. Tom Gallagher
16. J. Brooks Jackson
17. Anna E. Merrill
18. C. Michael Knudson
19. Grant D. Brown
20. Wendy Maury
21. Hillel Haim

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SARS-CoV-2 variants of concern have acquired mutations associated with an increased spike cleavage

1. Alba Escalera
2. Ana S. Gonzalez-Reiche
3. Sadaf Aslam
4. Ignacio Mena
5. Rebecca L. Pearl
6. Manon Laporte
7. Andrea Fossati
8. Raveen Rathnasinghe
9. Hala Alshammary
10. Adriana van de Guchte
11. Mehdi Bouhaddou
12. Thomas Kehrer
13. Lorena Zuliani-Alvarez
14. David A. Meekins
15. Velmurugan Balaraman
16. Chester McDowell
17. Jürgen A. Richt
18. Goran Bajic
19. Emilia Mia Sordillo
20. Nevan Krogan
21. Viviana Simon
22. Randy A. Albrecht
23. Harm van Bakel
24. Adolfo Garcia-Sastre
25. Teresa Aydillo

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