Microbiology

Spatial alanine metabolism determines local growth dynamics of Escherichia coli colonies

1. Francisco Díaz-Pascual
2. Martin Lempp
3. Kazuki Nosho
4. Hannah Jeckel
5. Jeanyoung K Jo
6. Konstantin Neuhaus
7. Raimo Hartmann
8. Eric Jelli
9. Mads Frederik Hansen
10. Alexa Price-Whelan
11. Lars EP Dietrich
12. Hannes Link
13. Knut Drescher

• Curated by eLife

  Evaluation Summary:

  In this manuscript the authors use a combination of transcriptomics, metabolomics, and quantitative measurements of growth to characterize the temporal and spatial distribution of cells with different metabolic states within colony of biofilms of the model bacterium Escherichia coli. They show that within the biofilm cells performing different metabolic functions are distributed in different regions of the colonies, and propose a model where nutrient cross-feeding through the amino acid alanine explains the phenotypic heterogeneity observed within the biofilm. The findings reported have potential broad implications for thinking about the spatial structure of communities of all bacterial species.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

Reviewed by eLife

Age-Related Susceptibility of Ferrets to SARS-CoV-2 Infection

1. Mathias Martins
2. Maureen H. V. Fernandes
3. Lok R. Joshi
4. Diego G. Diel

Reviewed by ScreenIT

Identification of potent small molecule inhibitors of SARS-CoV-2 entry

1. Sonia Mediouni
2. Huihui Mou
3. Yuka Otsuka
4. Joseph Anthony Jablonski
5. Robert Scott Adcock
6. Lalit Batra
7. Dong-Hoon Chung
8. Christopher Rood
9. Ian Mitchelle S. de Vera
10. Ronald Rahaim Jr.
11. Sultan Ullah
12. Xuerong Yu
13. Yulia A. Getmanenko
14. Nicole M. Kennedy
15. Chao Wang
16. Tu-Trinh Nguyen
17. Mitchell Hull
18. Emily Chen
19. Thomas D. Bannister
20. Pierre Baillargeon
21. Louis Scampavia
22. Michael Farzan
23. Susana T. Valente
24. Timothy P. Spicer

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CPC-containing oral rinses inactivate SARS-CoV-2 variants and are active in the presence of human saliva

1. Enyia R. Anderson
2. Edward I. Patterson
3. Siobhan Richards
4. Ana K. Pitol
5. Thomas Edwards
6. Dominic Wooding
7. Kate Buist
8. Alison Green
9. Sayandip Mukherjee
10. Michael Hoptroff
11. Grant L. Hughes

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Rapid assessment of SARS-CoV-2–evolved variants using virus-like particles

1. Abdullah M. Syed
2. Taha Y. Taha
3. Takako Tabata
4. Irene P. Chen
5. Alison Ciling
6. Mir M. Khalid
7. Bharath Sreekumar
8. Pei-Yi Chen
9. Jennifer M. Hayashi
10. Katarzyna M. Soczek
11. Melanie Ott
12. Jennifer A. Doudna

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Limited Variation between SARS-CoV-2-Infected Individuals in Domain Specificity and Relative Potency of the Antibody Response against the Spike Glycoprotein

1. Hanora A. Van Ert
2. Dana W. Bohan
3. Kai Rogers
4. Mohammad Fili
5. Roberth A. Rojas Chávez
6. Enya Qing
7. Changze Han
8. Spencer Dempewolf
9. Guiping Hu
10. Nathan Schwery
11. Kristina Sevcik
12. Natalie Ruggio
13. Devlin Boyt
14. Michael A. Pentella
15. Tom Gallagher
16. J. Brooks Jackson
17. Anna E. Merrill
18. C. Michael Knudson
19. Grant D. Brown
20. Wendy Maury
21. Hillel Haim

Reviewed by ScreenIT

SARS-CoV-2 variants of concern have acquired mutations associated with an increased spike cleavage

1. Alba Escalera
2. Ana S. Gonzalez-Reiche
3. Sadaf Aslam
4. Ignacio Mena
5. Rebecca L. Pearl
6. Manon Laporte
7. Andrea Fossati
8. Raveen Rathnasinghe
9. Hala Alshammary
10. Adriana van de Guchte
11. Mehdi Bouhaddou
12. Thomas Kehrer
13. Lorena Zuliani-Alvarez
14. David A. Meekins
15. Velmurugan Balaraman
16. Chester McDowell
17. Jürgen A. Richt
18. Goran Bajic
19. Emilia Mia Sordillo
20. Nevan Krogan
21. Viviana Simon
22. Randy A. Albrecht
23. Harm van Bakel
24. Adolfo Garcia-Sastre
25. Teresa Aydillo

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Contribution of single mutations to selected SARS-CoV-2 emerging variants spike antigenicity

1. Shang Yu Gong
2. Debashree Chatterjee
3. Jonathan Richard
4. Jérémie Prévost
5. Alexandra Tauzin
6. Romain Gasser
7. Yuxia Bo
8. Dani Vézina
9. Guillaume Goyette
10. Gabrielle Gendron-Lepage
11. Halima Medjahed
12. Michel Roger
13. Marceline Côté
14. Andrés Finzi

Reviewed by ScreenIT

Within-host evolutionary dynamics of seasonal and pandemic human influenza A viruses in young children

1. Alvin X Han
2. Zandra C Felix Garza
3. Matthijs RA Welkers
4. René M Vigeveno
5. Nhu Duong Tran
6. Thi Quynh Mai Le
7. Thai Pham Quang
8. Dinh Thoang Dang
9. Thi Ngoc Anh Tran
10. Manh Tuan Ha
11. Thanh Hung Nguyen
12. Quoc Thinh Le
13. Thanh Hai Le
14. Thi Bich Ngoc Hoang
15. Kulkanya Chokephaibulkit
16. Pilaipan Puthavathana
17. Van Vinh Chau Nguyen
18. My Ngoc Nghiem
19. Van Kinh Nguyen
20. Tuyet Trinh Dao
21. Tinh Hien Tran
22. Heiman FL Wertheim
23. Peter W Horby
24. Annette Fox
25. H Rogier van Doorn
26. Dirk Eggink
27. Menno D de Jong
28. Colin A Russell

• Curated by eLife

  Evaluation Summary:

  This work offers an in-depth exploration of within-host influenza evolution that helps untangle the process by which global trends arise from host-level dynamics. The manuscript will be of interest to virologists and evolutionary biologists alike, and the dataset provides a unique opportunity to explore evolutionary dynamics late in acute infections of both endemic and emerging viruses. Technical concerns relating to data interpretation and modeling assumptions suggest that some results might change after further investigation.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

SARS-CoV-2 B.1.351 (beta) variant shows enhanced infectivity in K18-hACE2 transgenic mice and expanded tropism to wildtype mice compared to B.1 variant

1. Ferran Tarrés-Freixas
2. Benjamin Trinité
3. Anna Pons-Grífols
4. Miguel Romero-Durana
5. Eva Riveira-Muñoz
6. Carlos Ávila-Nieto
7. Mónica Pérez
8. Edurne Garcia-Vidal
9. Daniel Pérez-Zsolt
10. Jordana Muñoz-Basagoiti
11. Dàlia Raïch-Regué
12. Nuria Izquierdo-Useros
13. Ignacio Blanco
14. Marc Noguera-Julián
15. Victor Guallar
16. Rosalba Lepore
17. Alfonso Valencia
18. Júlia Vergara-Alert
19. Bonaventura Clotet
20. Ester Ballana
21. Jorge Carrillo
22. Joaquim Segalés
23. Julià Blanco

Reviewed by ScreenIT