May a Strain Chlamydia Isolated From SARS Patient’s Autopsy Issues Inhibit the Proliferation of SARS-CoV? An Early Observation in Vitro
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Abstract
We found that a strain chlamydia isolated from SARS patient’s autopsy issues could decrease the proliferation of SARS-CoV in vitro; The inhibitory factors distribute both in the extracellular and intracellular cultures.
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SciScore for 10.1101/2021.08.27.457969: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics Field Sample Permit: 1.1 Virus, chlamydia and cells: SARS-CoV (BJ01 strain), the strain Chlamydia-1 (isolated from the autopsy specimens of SARS-CoV patients), and Human Embryo Kidney 293 Cells were kindly provided by Prof. Hong T (Chinese Center for Disease Control and Prevention, Beijing, China); the strain Chlamydia psittaci (C. psittaci) was purchased from the China veterinary Culture Collection Center (No.CVCC2410). Sex as a biological variable not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources The final titration of the strain Chlamydia-1 were … SciScore for 10.1101/2021.08.27.457969: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics Field Sample Permit: 1.1 Virus, chlamydia and cells: SARS-CoV (BJ01 strain), the strain Chlamydia-1 (isolated from the autopsy specimens of SARS-CoV patients), and Human Embryo Kidney 293 Cells were kindly provided by Prof. Hong T (Chinese Center for Disease Control and Prevention, Beijing, China); the strain Chlamydia psittaci (C. psittaci) was purchased from the China veterinary Culture Collection Center (No.CVCC2410). Sex as a biological variable not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources The final titration of the strain Chlamydia-1 were 1.2×105 IFUs/mL, and the final titration of the strain C. psittaci were 3.0×105 IFUs/mL. 1.2 Co-infection of SARS-CoV and chlamydia in 293 cells: For the co-infection of SARS-CoV and the strain Chlamydia-1: 293 cells were cultured in 200 uL dulbecco’s modified eagle’s medium (DMKM) supplemented with 10% fetal bovine serum (FBS) and 1% antibiotics/antimycotic, seeded in a 96-well cell plate with 1×105 cells per well (a 13 mm round coverslip was paved in each well in advance), and then kept in 5% CO2, saturated humidity, and 37°C. 293suggested: NoneSoftware and Algorithms Sentences Resources All of the statistical analyses were performed with statistical product and service solutions (SPSS) version 25.0. SPSSsuggested: (SPSS, RRID:SCR_002865)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
Results from scite Reference Check: We found no unreliable references.
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