Microbiology

Why SARS-CoV-2 Omicron variant is milder? A single high-frequency mutation of structural envelope protein matters

1. Bingqing Xia
2. Yi wang
3. Xiaoyan pan
4. Xi Cheng
5. Hongying Ji
6. Xiaoli Zuo
7. Jia Li
8. Zhaobing Gao

Reviewed by ScreenIT

Intracellular Flow Cytometry Complements RT-qPCR Detection of Circulating SARS-CoV-2 Variants of Concern

1. Emiel Vanhulle
2. Becky Provinciael
3. Joren Stroobants
4. Anita Camps
5. Piet Maes
6. Kurt Vermeire

Reviewed by ScreenIT

Interactions between strains govern the eco-evolutionary dynamics of microbial communities

1. Akshit Goyal
2. Leonora S Bittleston
3. Gabriel E Leventhal
4. Lu Lu
5. Otto X Cordero

• Curated by eLife

  Evaluation Summary:

  The authors attempt to derive a threshold of genetic distance, beyond which two microbial strains diverge in their behavior in an ecological community. The question is of broad interest to ecology, especially microbial ecology. To answer this question, the authors followed the population dynamics of individual strains derived from a natural microbial community under constant environmental conditions in the lab. The statistical framework could be improved, and a more rigorous account of how the phylogenetic inference of strains and species was made should be given.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

A pseudotyped lentivirus-based assay to titer SARS-CoV-2 neutralizing antibodies in Mexico

1. José Antonio Cruz-Cardenas
2. Michelle Gutierrez-Mayret
3. Alejandra López-Arredondo
4. Julio Enrique Castañeda-Delgado
5. Augusto Rojas-Martinez
6. Gerardo García-Rivas
7. José Antonio Enciso-Moreno
8. Laura A. Palomares
9. Marion E. G. Brunck

Reviewed by ScreenIT

Engineering SARS-CoV-2 cocktail antibodies into a bispecific format improves neutralizing potency and breadth

1. Zhiqiang Ku
2. Xuping Xie
3. Jianqing Lin
4. Peng Gao
5. Abbas El Sahili
6. Hang Su
7. Yang Liu
8. Xiaohua Ye
9. Xin Li
10. Xuejun Fan
11. Boon Chong Goh
12. Wei Xiong
13. Hannah Boyd
14. Antonio E. Muruato
15. Hui Deng
16. Hongjie Xia
17. Zou Jing
18. Birte K. Kalveram
19. Vineet D. Menachery
20. Ningyan Zhang
21. Julien Lescar
22. Pei-Yong Shi
23. Zhiqiang An

Reviewed by ScreenIT

Significant Broad Spectrum Antiviral activity of Bi121 Against Different Variants of SARS-CoV-2

1. Bobban Subhadra
2. Ragini Agrawal
3. Virender Kumar Pal
4. Agnes-Laurence Chenine
5. Jeffy George Mattathil
6. Amit Singh

Reviewed by ScreenIT

A booster dose of Delta × Omicron hybrid mRNA vaccine produced broadly neutralizing antibody against Omicron and other SARS-CoV-2 variants

1. I-Jung Lee
2. Cheng-Pu Sun
3. Ping-Yi Wu
4. Yu-Hua Lan
5. I-Hsuan Wang
6. Wen-Chun Liu
7. Joyce Pei-Yi Yuan
8. Yu-Wei Chang
9. Sheng-Che Tseng
10. Szu-I Tsung
11. Yu-Chi Chou
12. Monika Kumari
13. Yin-Shiou Lin
14. Hui-Feng Chen
15. Tsung-Yen Chen
16. Chih-Chao Lin
17. Chi-Wen Chiu
18. Chung-Hsuan Hsieh
19. Cheng-Ying Chuang
20. Chao-Min Cheng
21. Hsiu-Ting Lin
22. Wan-Yu Chen
23. Fu-Fei Hsu
24. Ming-Hsiang Hong
25. Chun-Che Liao
26. Chih-Shin Chang
27. Jian-Jong Liang
28. Hsiu-Hua Ma
29. Ming-Tsai Chiang
30. Hsin-Ni Liao
31. Hui-Ying Ko
32. Liang-Yu Chen
33. Yi-An Ko
34. Pei-Yu Yu
35. Tzu-Jing Yang
36. Po-Cheng Chiang
37. Shang-Te Hsu
38. Yi-Ling Lin
39. Chong-Chou Lee
40. Han-Chung Wu
41. Mi-Hua Tao

Reviewed by ScreenIT

Listeria monocytogenes requires cellular respiration for NAD+ regeneration and pathogenesis

1. Rafael Rivera-Lugo
2. David Deng
3. Andrea Anaya-Sanchez
4. Sara Tejedor-Sanz
5. Eugene Tang
6. Valeria M Reyes Ruiz
7. Hans B Smith
8. Denis V Titov
9. John-Demian Sauer
10. Eric P Skaar
11. Caroline M Ajo-Franklin
12. Daniel A Portnoy
13. Samuel H Light

• Curated by eLife

  Evaluation Summary:

  In their study, Rivera-Luogo et al. investigate the main role of respiration in Listeria monocytogenes pathogenicity, whether it is more important for redox balance control (ratio of balance NAD+/NADH) or generation of proton motive force. Drawing on results from an elegant heterologous NOX system supporting regeneration of NAD without producing proton motive force, the authors propose that NAD regeneration, i.e. redox balance, is more important for Listeria than proton motive force as a result of respiration. This is clearly a new way of perceiving respiration that should be of interest broadly to the microbiology community and broader readership.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #3 agreed to share their name with the authors.)

Reviewed by eLife

Distinct genetic determinants and mechanisms of SARS-CoV-2 resistance to remdesivir

1. Laura J. Stevens
2. Andrea J. Pruijssers
3. Hery W. Lee
4. Calvin J. Gordon
5. Egor P. Tchesnokov
6. Jennifer Gribble
7. Amelia S. George
8. Tia M. Hughes
9. Xiaotao Lu
10. Jiani Li
11. Jason K. Perry
12. Danielle P. Porter
13. Tomas Cihlar
14. Timothy P. Sheahan
15. Ralph S. Baric
16. Matthias Götte
17. Mark R. Denison

Reviewed by ScreenIT

Susceptibility of Wild Canids to SARS-CoV-2

1. Stephanie M. Porter
2. Airn E. Hartwig
3. Helle Bielefeldt-Ohmann
4. Angela M. Bosco-Lauth
5. J. Jeffrey Root

Reviewed by ScreenIT