Serological screening in wild ruminants in Germany, 2021/2022: No evidence of SARS‐CoV‐2, bluetongue virus or pestivirus spread but high seroprevalences against Schmallenberg virus

  1. Kerstin Wernike
  2. Luisa Fischer
  3. Mark Holsteg
  4. Andrea Aebischer
  5. Anja Petrov
  6. Katharina Marquart
  7. Ulrich Schotte
  8. Jacob Schön
  9. Donata Hoffmann
  10. Silke Hechinger
  11. Antonie Neubauer‐Juric
  12. Julia Blicke
  13. Thomas C. Mettenleiter
  14. Martin Beer

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Abstract

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  1. Version published to 10.1111/tbed.14600
  2. ScreenIT

    SciScore for 10.1101/2022.02.21.481262: (What is this?)

    Please note, not all rigor criteria are appropriate for all manuscripts.

    Table 1: Rigor

    Ethicsnot detected.
    Sex as a biological variablenot detected.
    Randomizationnot detected.
    Blindingnot detected.
    Power Analysisnot detected.
    Cell Line Authenticationnot detected.

    Table 2: Resources

    Antibodies
    SentencesResources
    Serological test systems: All samples collected in the 2021/22 hunting season were tested for the presence of anti-SBV antibodies by a glycoprotein Gc-based ELISA performed as described previously [42].
    anti-SBV
    suggested: None
    The latter allows for the detection of anti-BVDV antibodies as well as for antibodies against the ovine BDV.
    anti-BVDV
    suggested: None
    For the detection of antibodies directed against SARS-CoV-1, an ELISA system was established in line with the SARS-CoV-2 test.
    SARS-CoV-1
    suggested: None
    Experimental Models: Cell Lines
    SentencesResources
    Expi293 cells were grown in suspension in Expi293 expression medium (Thermo Fisher Scientific) at 37 °C, 8 % CO2 and 125 rpm.
    Expi293
    suggested: RRID:CVCL_D615)
    Recombinant DNA
    SentencesResources
    For expression of the recombinant protein, the RBD-SD1 domain (aa 306 – 577) of the SARS coronavirus strain Tor2 (NC_004718.3) was ordered as a synthetic DNA string fragment (GeneArt synthesis; Thermo Fisher Scientific, Darmstadt, Germany) and cloned into the pEXPR103 expression vector (IBA Lifesciences, Göttingen, Germany) in-frame with a C-terminal Strep-tag.
    pEXPR103
    suggested: None

    Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

    Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

    Results from TrialIdentifier: No clinical trial numbers were referenced.

    Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

    Results from JetFighter: We did not find any issues relating to colormaps.

    Results from rtransparent:
    • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
    • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
    • No protocol registration statement was detected.

    Results from scite Reference Check: We found no unreliable references.

    About SciScore

    SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.

  3. Version published to 10.1101/2022.02.21.481262 on bioRxiv