Microbiology

S-217622, a SARS-CoV-2 main protease inhibitor, decreases viral load and ameliorates COVID-19 severity in hamsters

1. Michihito Sasaki
2. Koshiro Tabata
3. Mai Kishimoto
4. Yukari Itakura
5. Hiroko Kobayashi
6. Takuma Ariizumi
7. Kentaro Uemura
8. Shinsuke Toba
9. Shinji Kusakabe
10. Yuki Maruyama
11. Shun Iida
12. Noriko Nakajima
13. Tadaki Suzuki
14. Shinpei Yoshida
15. Haruaki Nobori
16. Takao Sanaki
17. Teruhisa Kato
18. Takao Shishido
19. William W. Hall
20. Yasuko Orba
21. Akihiko Sato
22. Hirofumi Sawa

Reviewed by ScreenIT

A live-attenuated SARS-CoV-2 vaccine candidate with accessory protein deletions

1. Yang Liu
2. Xianwen Zhang
3. Jianying Liu
4. Hongjie Xia
5. Jing Zou
6. Antonio E. Muruato
7. Sivakumar Periasamy
8. Chaitanya Kurhade
9. Jessica A. Plante
10. Nathen E. Bopp
11. Birte Kalveram
12. Alexander Bukreyev
13. Ping Ren
14. Tian Wang
15. Vineet D. Menachery
16. Kenneth S. Plante
17. Xuping Xie
18. Scott C. Weaver
19. Pei-Yong Shi

Reviewed by ScreenIT

SARS-CoV-2 Permissive glioblastoma cell line for high throughput antiviral screening

1. Emiel Vanhulle
2. Joren Stroobants
3. Becky Provinciael
4. Anita Camps
5. Sam Noppen
6. Piet Maes
7. Kurt Vermeire

Reviewed by ScreenIT

Malaria parasites utilize pyrophosphate to fuel an essential proton pump in the ring stage and the transition to trophozoite stage

1. Omobukola Solebo
2. Liqin Ling
3. Jing Zhou
4. Tian-Min Fu
5. Hangjun Ke

• Curated by eLife

  Evaluation Summary:

  This work will be of interest to researchers seeking new ways to target malaria parasites. The work provides insight into the energy requirements of parasites during the first day of their two-day life cycle, a period during which they are significantly resistant to a wide variety of antimalarial drugs, and identifies an essential enzyme that could be targeted in early-stage parasites. The study shows this protein is necessary for normal development and growth of parasites in red blood cells.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #3 agreed to share their name with the authors.)

Reviewed by eLife

In Vitro Selection of Remdesivir-Resistant SARS-CoV-2 Demonstrates High Barrier to Resistance

1. Liva Checkmahomed
2. Julie Carbonneau
3. Venice Du Pont
4. Nicholas C. Riola
5. Jason K. Perry
6. Jiani Li
7. Bastien Paré
8. Shawn M. Simpson
9. Martin A. Smith
10. Danielle P. Porter
11. Guy Boivin

Reviewed by ScreenIT

Remdesivir and GS-441524 Retain Antiviral Activity against Delta, Omicron, and Other Emergent SARS-CoV-2 Variants

1. Jared Pitts
2. Jiani Li
3. Jason K. Perry
4. Venice Du Pont
5. Nicholas Riola
6. Lauren Rodriguez
7. Xianghan Lu
8. Chaitanya Kurhade
9. Xuping Xie
10. Gregory Camus
11. Savrina Manhas
12. Ross Martin
13. Pei-Yong Shi
14. Tomas Cihlar
15. Danielle P. Porter
16. Hongmei Mo
17. Evguenia Maiorova
18. John P. Bilello

Reviewed by ScreenIT

Dual Inhibition of Cathepsin L and 3CL-Pro by GC-376 Constrains SARS Cov2 Infection Including Omicron Variant

1. Prabhakaran Kumar
2. Kiira M Ratia
3. Justin M Richner
4. Gregory R J Thatcher
5. Rashmi Kadam
6. Sandra P Smieszek
7. Bartlomiej P Przychodzen
8. Vuk Koprivica
9. Gunther Birznieks
10. Mihael H Polymeropoulos
11. Bellur S Prabhakar

Reviewed by ScreenIT

Host casein kinase 1-mediated phosphorylation modulates phase separation of a rhabdovirus phosphoprotein and virus infection

1. Xiao-Dong Fang
2. Qiang Gao
3. Ying Zang
4. Ji-Hui Qiao
5. Dong-Min Gao
6. Wen-Ya Xu
7. Ying Wang
8. Dawei Li
9. Xian-Bing Wang

• Curated by eLife

  Evaluation Summary:

  Recently liquid-liquid phase separation (LLPS) has emerged as a mechanism by which membraneless compartments are formed inside the cells to compartmentalize biomolecules. In this paper, the authors show that the P protein from a plant-infecting negative sense RNA virus undergoes LLPS to promote virus replication. The host casein kinase 1 phosphorylates P protein and inhibits phase separation and viral replication. This paper will be of interest to virologists and researchers who study LLPS.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #3 agreed to share their name with the authors.)

Reviewed by eLife

Noisy metabolism can promote microbial cross-feeding

1. Jaime G Lopez
2. Ned S Wingreen

• Curated by eLife

  Evaluation Summary:

  This paper will be of broad interest to microbiologists interested in gene expression noise and/or metabolic interactions in microbial communities. It provides a novel hypothesis that complements existing theoretical frameworks. The hypothesis is well supported by data from a mathematical model, and it its predictions could be tested experimentally in future work.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

N-acylethanolamine acid amide hydrolase is a novel target for drugs against SARS-CoV-2 and Zika virus

1. Michele Lai
2. Veronica La Rocca
3. Rachele Amato
4. Elena Iacono
5. Carolina Filipponi
6. Elisa Catelli
7. Lucia Bogani
8. Rossella Fonnesu
9. Giulia Lottini
10. Alessandro De Carli
11. Alessandro Mengozzi
12. Stefano Masi
13. Paola Quaranta
14. Pietro Giorgio Spezia
15. Giulia Freer
16. Paola Lenzi
17. Francesco Fornai
18. Daniele Piomelli
19. Mauro Pistello

Reviewed by ScreenIT