Immunology

Env-antibody coevolution identifies B cell priming as the principal bottleneck to HIV-1 V2 apex broadly neutralizing antibody development

1. Rumi Habib
2. Ryan S. Roark
3. Hui Li
4. Andrew Jesse Connell
5. Michael P. Hogarty
6. Kshitij Wagh
7. Shuyi Wang
8. Lorie Marchitto
9. Ashwin N. Skelly
10. John W. Carey
11. Kirsten J. Sowers
12. Kasirajan Ayyanathan
13. Samantha J. Plante
14. Frederic Bibollet-Ruche
15. Younghoon Park
16. Colby J. Agostino
17. Ajay Singh
18. Christian L. Martella
19. Emily Lewis
20. Juliette M. Rando
21. Neha Chohan
22. Jinery Lora
23. Wenge Ding
24. Mary S. Campion
25. Chengyan Zhao
26. Weimin Liu
27. Yingying Li
28. Xuduo Li
29. Bo Liang
30. Rohan Roy Chowdhury
31. Khaled Amereh
32. Elizabeth Van Itallie
33. Zizhang Sheng
34. Amrit R. Ghosh
35. Katharine J. Bar
36. Wilton B. Williams
37. Kevin Wiehe
38. Kevin O. Saunders
39. Robert J. Edwards
40. Derek W. Cain
41. Mark Lewis
42. Facundo D. Batista
43. Dennis R. Burton
44. Raiees Andrabi
45. Daniel W. Kulp
46. Barton F. Haynes
47. Bette Korber
48. Lawrence Shapiro
49. Peter D. Kwong
50. Beatrice H. Hahn
51. George M. Shaw

Reviewed by Rapid Reviews Infectious Diseases

NK cells undergo transcriptional and functional reprogramming following Streptococcus pneumoniae infection

1. Júlia Torné
2. Claudia Chica
3. Tiphaine Camarasa
4. Bernd Jagla
5. Matilde Enes Dias
6. Aymeric Zellner
7. Sébastien Mella
8. Valentina Libri
9. Mélanie Anne Hamon

Reviewed by Rapid Reviews Infectious Diseases, PREreview

Alterations in Circulating T-Cell Subsets with Gut-Homing/Residency Phenotypes Predict HIV-1 Status and Subclinical Atherosclerosis

1. Etiene Moreira Gabriel
2. Jonathan Dias
3. Abdelali Filali-Mouhim
4. Ramon Edwin Caballero
5. Tomas Raul Wiche Salinas
6. Manon Nayrac
7. Carl Chartrand-Lefebvre
8. Jean-Pierre Routy
9. Madeleine Durand
10. Mohamed El-Far
11. Cécile Tremblay
12. Petronela Ancuta

Reviewed by Rapid Reviews Infectious Diseases

The 1000+ mouse project for large-scale spatiotemporal parametrization and modeling of preclinical cancer immunotherapies

1. Adam L Kenet
2. Sooraj Achar
3. Alka Dwivedi
4. John Buckley
5. Marie Pouzolles
6. Haying Qin
7. Christopher Chien
8. Naomi Taylor
9. Grégoire Y Altan-Bonnet

• Curated by eLife

  eLife Assessment

  The authors developed a fundamental computational method, which is intended to automatically process bioluminescence imaging-derived tumour images across anatomical regions and over time. This allows quantitative analysis of such data, and the authors applied it to describe the spatiotemporal distribution of tumour cells in response to CD19-targeted CAR-T cells that contained either CD28 or 4-1BB costimulatory domains. Some operational limitations were identified, which relate to the pipeline's reliance on predefined regions of interest instead of aligning signal sites with anatomical information, scaling, and limitations in taking animal pose into account. Overall, the authors provide compelling evidence for the functionality of their computational approach towards automated analysis of bioluminescence imaging data, while applying it to a current topic of wide interest in cell therapy research.

Reviewed by eLife

Cas9+ conditionally immortalized neutrophil progenitors as a tool for genome-wide CRISPR screening for neutrophil differentiation and function

1. Robyn M Jong
2. Krystal L Ching
3. Nicholas E Garelis
4. Alex Zilinskas
5. Xammy Huu Wrynla
6. Sagar Rawal
7. Bianca C Hill
8. Bridget A Luckie
9. Lillian Shallow
10. Jeffery S Cox
11. Gregory M Barton
12. Sarah A Stanley

• Curated by eLife

  eLife Assessment

  In this manuscript, Jong et al. provide and validate a very useful resource for performing CRISPR screenings to study neutrophil differentiation and function by generating Hoxb8 cells that constitutively express Cas9. This library-screening approach has the potential to improve on the established lentiviral CRISPR-Cas9 editing of Hoxb8 cells. However, the technical advances provided are only incremental and the results presented in this study do not significantly further our understanding of these cells, but rather provide a good validation of their Cas9+ modified version.

Reviewed by eLife

Predestined neutrophil heterogeneity in homeostasis varies in transcriptional and phenotypic response to Candida

1. Allison K. Scherer
2. Alex Hopke
3. Shuying Xu
4. Adam Viens
5. Natalie J. Alexander
6. Kyle D. Timmer
7. Dakota Archambault
8. Daniel Floyd
9. Natalie J. Atallah
10. Catherine Rhee
11. Murat Cetinbas
12. David T. Scadden
13. Daniel Irimia
14. David B. Sykes
15. Ruslan I. Sadreyev
16. Michael K. Mansour

• Curated by eLife

  eLife Assessment

  In their study, Scherer and colleagues aim to use analyses of single-cell clones of murine granulocyte monocyte progenitors that are conditionally immortalized, and analyses of neutrophils derived from those clones to characterize an experimental system for studying neutrophil heterogeneity. The multi-omic and functional analyses reported are valuable but the strength of the evidence presented in support of them is incomplete because the study lacks a rigorous demonstration that the neutrophil-like cells that they derive are fully mature neutrophils.

Reviewed by eLife

Tolerance to Lung Infection in TWIK2 K+ Efflux Mediated Macrophage Trained Immunity

1. Josh Thompson
2. Yufan Li
3. Yuanling Song
4. Anas Anas
5. Jaewon Cho
6. Ki-Wook Kim
7. Asrar B Malik
8. Jingsong Xu

• Curated by eLife

  eLife Assessment

  This study presents valuable data suggesting that ATP-induced modulation of alveolar macrophage (AM) functions is associated with NLRP3 inflammasome activation and enhanced phagocytic capacity. While the in vivo and in vitro data reveal an interesting phenotype, the evidence provided is incomplete and does not fully support the paper's conclusions. Additional investigations would be of value in complementing the data and strengthening the interpretation of the results. This study should be of interest to immunologists and the mucosal immunity community.

Reviewed by eLife

An IL-21R hypomorph circumvents functional redundancy to define STAT1 signaling in germinal center responses

1. Christoph Jandl
2. Joanna Warren
3. Samantha Owens
4. Marcel Batten
5. Howard Wang
6. Cecile King

• Curated by eLife

  eLife Assessment

  IL21R, being a key cytokine receptor for shaping the T follicular helper and B cell functions, utilizes two STAT family members, STAT1 and STAT3. The authors utilize the IL21R ENU-induced mutant, together with relevant in vitro and in vivo experiments, to dissect the function of STAT1 and STAT3. The approach by itself sounds reasonable, but the main conclusions are incompletely supported by the data presented in this manuscript.

Reviewed by eLife

Innate immune responses to Plasmodium falciparum disrupt the blood-brain barrier

1. Alina Batzilla
2. Pia Dernick
3. Jon Bezney
4. Andreas R. Gschwind
5. Fumio Nakaki
6. Mireia Altadill
7. Hannah Fleckenstein
8. Livia Piatti
9. Silvia Sanz Sender
10. Manuel Fiegl
11. Olawunmi Rashidat Oyerinde
12. Borja Lopez Gutierrez
13. Miguel Lopez-Botet
14. James Sharpe
15. Lars Steinmetz
16. Gemma Moncunill
17. Maria Bernabeu

Reviewed by Rapid Reviews Infectious Diseases

Drosophila host defense mechanisms against filamentous fungal pathogens with diverse lifestyles

1. Guiqing Liu
2. Yao Tian
3. Mark Austin Hanson
4. Prince Kumar Sah
5. Jun Li
6. Bruno Lemaitre

Reviewed by Review Commons