Immunology

Synovial macrophage diversity and activation of M-CSF signaling in post-traumatic osteoarthritis

1. Alexander J Knights
2. Easton C Farrell
3. Olivia M Ellis
4. Michelle J Song
5. C Thomas Appleton
6. Tristan Maerz

• Curated by eLife

  eLife assessment

  This study provides useful information by identifying the cell type (macrophages) in synovial tissues involved in the pathogenesis of post-traumatic osteoarthritis (OA) and clarifying distinct transcriptomic signatures that may be a good therapeutic target for OA. However, the analysis performed so far is incomplete, with a main weakness being the lack of data to confirm the authors' speculation about the underlying mechanisms.

Reviewed by eLife

Acquisition of an immunosuppressive microenvironment after CAR-T therapy drives T-cell dysfunction and resistance

1. Marianna Ponzo
2. Lorenzo Drufuca
3. Chiara Buracchi
4. Marco M. Sindoni
5. Silvia Nucera
6. Cristina Bugarin
7. Ramona Bason
8. Grazisa Rossetti
9. Raoul Bonnal
10. Cristian Meli
11. Benedetta Rambaldi
12. Federico Lussana
13. Silvia Ferrari
14. Alex Moretti
15. Giulia Risca
16. Christian Pellegrino
17. Markus G. Manz
18. Stefania Galimberti
19. Alessandro Rambaldi
20. Giuseppe Gaipa
21. Andrea Biondi
22. Massimiliano Pagani
23. Chiara F. Magnani

Reviewed by PREreview

T3SS translocon induces pyroptosis by direct interaction with NLRC4/NAIP inflammasome

1. Yan Zhao
2. Hanshuo Zhu
3. Jinqian Li
4. Hang Xu
5. Li Sun

• Curated by eLife

  eLife Assessment

  This important study shows that Type 3 secretion translocons in Edwardsiella tarda and other bacteria activate the NAIP-NLRC4 inflammasome. The data from cellular and biochemical experiments showing that EseB is required for activation of the NLRC4 inflammasome are convincing. This paper is broadly relevant to those investigating host-pathogen interactions in diverse organisms.

Reviewed by eLife

Phosphoglycerate mutase regulates Treg differentiation through control of serine synthesis and one-carbon metabolism

1. Wesley H Godfrey
2. Kaho Cho
3. Xiaojing Deng
4. Chandra Shekar R Ambati
5. Vasanta Putluri
6. Abu Hena Mostafa Kamal
7. Nagireddy Putluri
8. Michael D Kornberg

• Curated by eLife

  eLife Assessment

  The results highlight an important physiological function of PGAM in the differentiation and suppressive activity of Treg cells by regulating serine synthesis. This role is proposed to intersect with glycolysis and one-carbon metabolism. Although the study's conclusion is supported by solid evidence from in-vitro cellular and in-vivo mouse models, there are some weaknesses and the reviewers suggested ways to improve the manuscript.

Reviewed by eLife

A VgrG2b fragment cleaved by caspase-11/4 promotes Pseudomonas aeruginosa infection through suppressing the NLRP3 inflammasome

1. Yan Qian
2. Qiannv Liu
3. Xiangyun Cheng
4. Chunlei Wang
5. Chun Kong
6. Mengqian Li
7. Chao Ren
8. Dong Jiang
9. Shuo Wang
10. Pengyan Xia

• Curated by eLife

  eLife Assessment

  This valuable study on Pseudomonas subverting host immunity identifies a new immune evasion strategy. There is solid evidence for the cleavage of VgrG2B by Caspase 11 and the generation of fragments that inhibit activity of the NLRP3 inflammasome. This work should be of interest to immunologists and microbiologists.

Reviewed by eLife

C-C chemokine receptor 4 deficiency exacerbates early atherosclerosis in mice

1. Toru Tanaka
2. Naoto Sasaki
3. Aga Krisnanda
4. Hilman Zulkifli Amin
5. Ken Ito
6. Sayo Horibe
7. Kazuhiko Matsuo
8. Ken-ichi Hirata
9. Takashi Nakayama
10. Yoshiyuki Rikitake

• Curated by eLife

  eLife Assessment

  This valuable study provides in-vivo evidence that CCR4 regulates the early inflammatory response during atherosclerotic plaque formation. The authors propose that altered T-cell response plays a role in this process, shedding light on mechanisms that may be of interest to medical biologists, biochemists, cell biologists, and immunologists. The work is currently considered incomplete pending textual changes and the inclusion of proper controls.

Reviewed by eLife

Fish CDK2 recruits Dtx4 to degrade TBK1 through ubiquitination in the antiviral response

1. Long-Feng Lu
2. Can Zhang
3. Zhuo-Cong Li
4. Bao-Jie Cui
5. Yang-Yang Wang
6. Ke-Jia Han
7. Xiao Xu
8. Chu-Jing Zhou
9. Xiao-Yu Zhou
10. Yue Wu
11. Na Xu
12. Xiao-Li Yang
13. Dan-Dan Chen
14. Xi-Yin Li
15. Li Zhou
16. Shun Li

• Curated by eLife

  eLife Assessment

  This valuable study uses zebrafish as a model to reveal a role for the cell cycle protein kinase CDK2 as a negative regulator of type I interferon signaling. The evidence supporting the authors' claims is convincing, including both in vivo and in vitro investigative approaches that corroborate a role for CDK2 in regulating TBK1 degradation. In this latest version, the authors included data addressing concerns raised by the reviewers at the first peer review round that strengthens the conclusions. This work will interest cell biologists, immunologists, and virologists.

Reviewed by eLife

Interleukin-1 prevents SARS-CoV-2-induced membrane fusion to restrict viral transmission via induction of actin bundles

1. Xu Zheng
2. Shi Yu
3. Yanqiu Zhou
4. Kuai Yu
5. Yuhui Gao
6. Mengdan Chen
7. Dong Duan
8. Yunyi Li
9. Xiaoxian Cui
10. Jiabin Mou
11. Yuying Yang
12. Xun Wang
13. Min Chen
14. Yaming Jiu
15. Jincun Zhao
16. Guangxun Meng

• Curated by eLife

  eLife Assessment

  This study provides important insights into how IL-1 cytokines protect cells against SARS-CoV-2 infection. By inducing a non-canonical RhoA/ROCK signaling pathway, IL-1beta inhibits the ability of SARS-CoV-2 infected cells to fuse with uninfected cells and produce syncytia. Convincing evidence underlies the identification of the key signaling components required for this inhibitory phenotype, and suggests that this process may also function to inhibit SARS-CoV-2 infection in vivo.

Reviewed by eLife

Hepatitis E Virus-induced antiviral response by plasmacytoid dendritic cells is modulated by the ORF2 protein

1. Garima Joshi
2. Elodie Décembre
3. Jacques Brocard
4. Claire Montpellier
5. Martin Ferrié
6. Omran Allatif
7. Ann-Kathrin Mehnert
8. Johann Pons
9. Delphine Galiana
10. Viet Loan Dao Thi
11. Nolwenn Jouvenet
12. Laurence Cocquerel
13. Marlène Dreux

Reviewed by Review Commons

B cell expression of the enzyme PexRAP, an intermediary in ether lipid biosynthesis, promotes antibody responses and germinal center size

1. Sung Hoon Cho
2. Marissa A Jones
3. Kaylor Meyer
4. David M Anderson
5. Sergei Chetyrkin
6. M Wade Calcutt
7. Richard M Caprioli
8. Clay F Semenkovich
9. Mark R Boothby

• Curated by eLife

  eLife Assessment

  This study provides useful insights into the ways in which germinal center B cell metabolism, particularly lipid metabolism, affects cellular responses. The authors use sophisticated mouse models to demonstrate that ether lipids are relevant for B cell homeostasis and efficient humoral responses. Although the data were collected from in vitro and in vivo experiments and analyzed using solid and validated methodology, more careful experiments and extensive revision of the manuscript will be required to strengthen the authors' conclusions.

Reviewed by eLife