Immunology

Label-Free In-Line Characterization of Immune Cell Culture using Quantitative Phase Imaging

1. Caroline E. Serafini
2. Viswanath Gorti
3. Paloma Casteleiro Costa
4. Aaron D. Silva Trenkle
5. Bharat Kanwar
6. Bryan Wang
7. Brian Wicker
8. Linda E. Kippner
9. Isaac LeCompte
10. Rui Qi Chen
11. Benjamin Joffe
12. Ye Li
13. Annie C. Bowles-Welch
14. Jing Li
15. Christine E. Brown
16. Gabriel A. Kwong
17. Stephen Balakirsky
18. Krishnendu Roy
19. Francisco E. Robles

Reviewed by Arcadia Science

The synovial lining macrophage layer develops in the first weeks of life in a CSF1- and TGFβ-dependent but monocyte-independent process

1. Marlene Magalhaes Pinto
2. Bert Malengier-Devlies
3. Guillaume Seuzaret
4. Anna Ahlback
5. Solvig Becker
6. Katelyn Patatsos
7. Georgios Drakoulis
8. Julia Karjalainen
9. Christiane Ruedl
10. David Voehringer
11. Calum C Bain
12. Elaine Emmerson
13. Barbora Schonfeldova
14. Kristina Zec
15. Irina Udalova
16. Theodoros Simakou
17. Lucy MacDonald
18. Mariola Kurowska-Stolarska
19. Jadwiga Zarebska
20. Tonia Vincent
21. Romeo Ricci
22. Eric Erbs
23. Jack Barrington
24. Barry W McColl
25. Georgiana Neag
26. Samuel Kemble
27. Christopher Mahony
28. Adam Croft
29. Louis Boon
30. Nicole Migotsky
31. Megan L Killian
32. Oumaima Ben Brahim
33. Stefan Uderhardt
34. Alexandre Gallerand
35. Stoyan Ivanov
36. Rebecca Gentek

Reviewed by Review Commons

Inhibition of the UFD-1-NPL-4 complex triggers an aberrant immune response in Caenorhabditis elegans

1. Rajneesh Rao
2. Alejandro Aballay
3. Jogender Singh

• Curated by eLife

  eLife Assessment

  In this valuable manuscript, Rao and colleagues investigate the UFD-1/NPL-4 complex, which is involved in extracting misfolded proteins in the plasma membrane and the accumulation of pathogenic bacteria in the intestine. Using convincing methods, the authors find that knockdown of the ufd-1 and npl-4 genes leads to shortened lifespan of the nematode C. elegans and reduced accumulation of the bacterial pathogen P. aeruginosa in the intestine.

Reviewed by eLife

IL-27 limits HSPC differentiation during infection and protects from stem cell exhaustion

1. Daniel L Aldridge
2. Zachary Lanzar
3. Anthony T Phan
4. David A Christian
5. Ryan D Pardy
6. Booki Min
7. Ross Kedl
8. Christopher A Hunter

• Curated by eLife

  eLife Assessment

  The article presents important findings describing the role of IL27 in maintaining HSCs at steady state, and in emergency haematopoiesis in response to T. goodii by limiting the inflammatory monocyte outcomes. The evidence provided are solid and support that IL27 acts at the level of HSCs and not downstream. This study will be of interest to immunologists and hematologists, as well as infectious disease researchers.

Reviewed by eLife

β-glucan reprograms macrophages to attenuate efferocytosis of cancer cells

1. Alexandros Chatzis
2. Jakub Lukaszonek
3. Dimitris Lagos
4. Dave Boucher
5. Ioannis Kourtzelis

• Curated by eLife

  eLife Assessment

  This important study describes the effect of beta-glucan innate training of macrophages and its effect on uptake of tumour cells and on the production of inflammatory cytokines. The data are convincing and show decreased phagocytic activity of apoptotic tumour cells accompanied by lower levels of secreted IL-1β, and in vivo findings are also provided in the revision. This finding has potential impact on designing potential macrophage-targeted cancer immuno-therapeutic approaches.

Reviewed by eLife

Tuberculosis susceptibility in genetically diverse mice reveals functional diversity of neutrophils

1. Marietta M Ravesloot-Chavez
2. Erik Van Dis
3. Douglas Fox
4. Andrea Anaya-Sanchez
5. Scott Espich
6. Xammy Huu Nguyenla
7. Sagar Rawal
8. Helia Samani
9. Mallory Ballinger
10. Henry F Thomas
11. Dmitri I Kotov
12. Russell E Vance
13. Michael W Nachman
14. Sarah A Stanley

• Curated by eLife

  eLife Assessment

  This study provides valuable insights into the host's variable susceptibility to Mycobacterium tuberculosis, using a novel collection of wild-derived inbred mouse lines from diverse geographic locations, along with immunological and single-cell transcriptomic analyses. While the data are convincing, a deeper mechanistic investigation into neutrophil subset functions would have further enhanced the study. This work will interest microbiologists and immunologists in the tuberculosis field.

Reviewed by eLife

Lipid peroxidation and type I interferon coupling fuels pathogenic macrophage activation causing tuberculosis susceptibility

1. Shivraj M Yabaji
2. Vadim Zhernovkov
3. Prasanna Babu Araveti
4. Suruchi Lata
5. Oleksii S Rukhlenko
6. Salam Al Abdullatif
7. Arthur Vanvalkenburg
8. Yuriy O Alekseyev
9. Qicheng Ma
10. Gargi Dayama
11. Nelson C Lau
12. W Evan Johnson
13. William R Bishai
14. Nicholas A Crossland
15. Joshua D Campbell
16. Boris N Kholodenko
17. Alexander A Gimelbrant
18. Lester Kobzik
19. Igor Kramnik

• Curated by eLife

  eLife Assessment

  Yabaji et al. reports a fundamental study highlighting the mechanistic connection for susceptibility to TB infection via the sst1 locus, this was shown to involve increased IFN and Myc production causing the down-regulation of anti-oxidant defence genes and chronic lipidation. Ultimately, lipid peroxidation may underlie infectivity and macrophage dysfunction. Overall, the data presented are compelling, supported by a well designed multi-omics approach and the findings will be of broad interest to researchers investigating the molecular mechanisms of TB infection.

  [Editors' note: this paper was reviewed by Review Commons.]

Reviewed by eLife, Review Commons

Nociceptor Neurons Control Pollution-Mediated Neutrophilic Asthma

1. Jo-Chiao Wang
2. Amelia Kulle
3. Theo Crosson
4. Amin Reza Nikpoor
5. Surbhi Gupta
6. Anais Roger
7. Moutih Rafei
8. Ajitha Thanabalasuriar
9. Sebastien Talbot

• Curated by eLife

  eLife Assessment

  This important work shows that fine particulate matter exposure to the lungs led to nociceptor-dependent neutrophilic inflammation. Likely macrophage-neuronal crosstalk, via release of artemin from macrophages and activation of Gfra3 on the JNC neuron, potentiated the response. The data convincingly strengthens links between pollutants, immune and neural interactions.

Reviewed by eLife

Interleukin 10 controls the balance between tolerance, pathogen elimination, and immunopathology in birds

1. Dominique Meunier
2. Ricardo Corona-Torres
3. Kay Boulton
4. Zhiguang Wu
5. Maeve Ballantyne
6. Laura Glendinning
7. Anum Ali Ahmad
8. Dominika Borowska
9. Lorna Taylor
10. Lonneke Vervelde
11. Jorge del Pozo
12. Marili Vasilogianni
13. José Jaramillo-Ortiz
14. Gonzalo Sanchez-Arsuaga
15. Androniki Psifidi
16. Fiona Tomley
17. Kellie A Watson
18. Michael J McGrew
19. Mark P Stevens
20. Damer P Blake
21. David A Hume

• Curated by eLife

  eLife Assessment

  IL-10 balances protective and deleterious immune functions in mice and humans, but if IL-10 also controls avian intestinal homeostasis remains unclear. Generating genetic knockouts, Meunier et al. established that a complete lack of IL-10 strengthened immunity against enteric bacteria in chickens, while also aggravating infection-inflicted inflammatory tissue damage and dysbiosis upon parasite infection, but unlike mouse models, IL-10 deficiency did not provoke spontaneous colitis in chickens. The findings presented are valuable, and the strength of evidence is convincing. The observation may have implications for the livestock industry and additional studies involving genetic knockouts may further unravel conserved and distinct avian IL-10 controls.

Reviewed by eLife

Beta-Glucan Modulates Monocyte Plasticity and Differentiation Capacity to Mitigate DSS-Induced Colitis

1. Yinyin Lv
2. Yanyun Fan
3. Qingxiang Gao
4. Qiongyun Chen
5. Yiqun Hu
6. Lin Wang
7. Huaxiu Shi
8. Ermei Chen
9. Qinyu Xu
10. Ying Cai
11. Qingqi Fan
12. Linying Li
13. Dan Du
14. Jianlin Ren
15. Shih-Chin Cheng
16. Hongzhi Xu

• Curated by eLife

  eLife Assessment

  This study presents valuable and compelling evidence that β-glucan-induced trained immunity can protect against intestinal inflammation by reprogramming innate immune cells toward a reparative phenotype. The authors employ a convincing combination of functional assays, adoptive transfers, and single-cell transcriptomics to uncover mechanistic insights and demonstrate the therapeutic potential of innate immune memory in IBD. While the work is robust, addressing the underlying epigenetic mechanisms and including additional controls would further reinforce the trained immunity-specific interpretation.

Reviewed by eLife