Immunology

Analysis of allelic cross-reactivity of monoclonal IgG antibodies by a multiplexed reverse FluoroSpot assay

1. Henriette Hoffmann-Veltung
2. Nsoh Godwin Anabire
3. Michael Fokuo Ofori
4. Peter Janhmatz
5. Niklas Ahlborg
6. Lars Hviid
7. Maria del Pilar Quintana

• Curated by eLife

  Evaluation Summary:

  This manuscript describes a fluorospot-based assay as a model for a methodical, step-wise and rigorous approach - that combines multiple reagents in a complex system - to study the cross-reactivity of antibody to polymorphic antigens using the malaria vaccine candidate, VAR2CSA, as a model. The authors apply monoclonal antibodies and the corresponding B cells to validate their multiplexed assay before testing small number of samples from malaria exposed donors in a pilot application of the assay. The data support the conclusions. This information will attract the attention of immunologists and vaccinologists, who are primarily but not exclusively, involved in research on malaria.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their name with the authors.)

Reviewed by eLife

Engineered natural killer cells impede the immunometabolic CD73-adenosine axis in solid tumors

1. Andrea M Chambers
2. Kyle B Lupo
3. Jiao Wang
4. Jingming Cao
5. Sagar Utturkar
6. Nadia Lanman
7. Victor Bernal-Crespo
8. Shadia Jalal
9. Sharon R Pine
10. Sandra Torregrosa-Allen
11. Bennett D Elzey
12. Sandro Matosevic

• Curated by eLife

  Evaluation Summary:

  The authors have engineered an anti-CD73 chimeric antigen receptor (CAR) that they express in NK cells to counteract tumors bearing CD73, which contributes to the generation of immunosuppressive adenosine in the tumor microenvironment. This is a promising approach for a new anti-cancer immunotherapy and will be of interest to oncologists and cancer immunologists. The CAR-bearing NK cells show slightly enhanced tumor killing in vitro, but preliminary data show more promising results in mice. This could be due to the CD73 CAR blocking catalytic activity in the tumor microenvironment more effectively than directly promoting cytotoxicity responses.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

Reviewed by eLife

The cIAP ubiquitin ligases sustain type 3 γδ T and innate lymphoid cells during aging to allow normal cutaneous and mucosal responses

1. John Rizk
2. Urs M. Mörbe
3. Rasmus Agerholm
4. Isabel Ulmert
5. Elisa Catafal Tardos
6. Darshana Kadekar
7. Maria V. Baglioni
8. Monica Torrellas Viñals
9. Vasileios Bekiaris

Reviewed by Review Commons

Neuron-associated macrophage proliferation in the sensory ganglia is associated with peripheral nerve injury-induced neuropathic pain involving CX3CR1 signaling

1. Rafaela M Guimarães
2. Conceição E Aníbal-Silva
3. Marcela Davoli-Ferreira
4. Francisco Isaac F Gomes
5. Atlante Mendes
6. Maria CM Cavallini
7. Miriam M Fonseca
8. Samara Damasceno
9. Larissa P Andrade
10. Marco Colonna
11. Cyril Rivat
12. Fernando Q Cunha
13. José C Alves-Filho
14. Thiago M Cunha

• Curated by eLife

  Evaluation Summary:

  Guimaraes et al address the origin of the macrophage increase in sensory ganglia after peripheral nerve injury. The authors show that there is no major influx by blood-derived monocytes into ganglia after injury and that resident macrophages proliferate, which is dependent on CX3CR1 signaling. Overall the work is clear and sound and should be of interest to immunologists and neurobiologists.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 and Reviewer #3 agreed to share their names with the authors.)

Reviewed by eLife

The Nucleocapsid Protein Of SARS-CoV-2, Combined With ODN-39M, Is A Potential Component For An Intranasal Bivalent Pancorona Vaccine

1. Yadira Lobaina
2. Rong Chen
3. Edith Suzarte
4. Panchao Ai
5. Vivian Huerta
6. Alexis Musacchio
7. Ricardo Silva
8. Changyuan Tan
9. Alejandro Martin
10. Laura Lazo
11. Gerardo Guillén
12. Ke Yang
13. Yasser Perera
14. Lisset Hermida

Reviewed by ScreenIT

Antiviral immune responses, cellular metabolism and adhesion are differentially modulated by SARS-CoV-2 ORF7a or ORF7b

1. Tránsito García-García
2. Raúl Fernández-Rodríguez
3. Natalia Redondo
4. Ana de Lucas-Rius
5. Sara Zaldívar-López
6. Blanca Dies López-Ayllón
7. José M. Suárez-Cárdenas
8. Ángeles Jiménez-Marín
9. María Montoya
10. Juan J. Garrido

Reviewed by ScreenIT

Plasmacytoid dendritic cells regulate megakaryocyte and platelet homeostasis

1. Florian Gaertner
2. Hellen Ishikawa-Ankerhold
3. Susanne Stutte
4. Wenwen Fu
5. Chenglong Guo
6. Jutta Weitz
7. Anne Dueck
8. Zhe Zhang
9. Dominic van den Heuvel
10. Valeria Fumagalli
11. Michael Lorenz
12. Louisa von Baumgarten
13. Konstantin Stark
14. Tobias Straub
15. Saskia von Stillfried
16. Peter Boor
17. Marco Colonna
18. Christian Schulz
19. Thomas Brocker
20. Barbara Walzog
21. Christoph Scheiermann
22. Stefan Engelhardt
23. William C. Aird
24. Tobias Petzold
25. Michael Sixt
26. Martina Rudelius
27. Claus Nerlov
28. Matteo Iannacone
29. Robert A. J. Oostendorp
30. Steffen Massberg

Reviewed by ScreenIT

Potentiating the cross-reactive IFN-γ T cell and polyfunctional T cell responses by heterologous GX-19N DNA booster in mice primed with either a COVID-19 mRNA vaccine or inactivated vaccine

1. Yong Bok Seo
2. Ara Ko
3. Duckhyang Shin
4. Junyoung Kim
5. You Suk Suh
6. Juyoung Na
7. Ji In Ryu
8. Young Chul Sung

Reviewed by ScreenIT

Respiratory mucosal vaccination of peptide-poloxamine-DNA nanoparticles provides complete protection against lethal SARS-CoV-2 challenge

1. Si Sun
2. Entao Li
3. Gan Zhao
4. Jie Tang
5. Qianfei Zuo
6. Larry Cai
7. Chuanfei Xu
8. Cheng Sui
9. Yangxue Ou
10. Chang Liu
11. Haibo Li
12. Yuan Ding
13. Chao Li
14. Dongshui Lu
15. Weijun Zhang
16. Ping Luo
17. Ping Cheng
18. Yuwei Gao
19. Changchun Tu
20. Bruno Pitard
21. Joseph Rosenecker
22. Bin Wang
23. Yan Liu
24. Quanming Zou
25. Shan Guan

Reviewed by ScreenIT

Dendritic cell Piezo1 directs the differentiation of TH1 and Treg cells in cancer

1. Yuexin Wang
2. Hui Yang
3. Anna Jia
4. Yufei Wang
5. Qiuli Yang
6. Yingjie Dong
7. Yueru Hou
8. Yejin Cao
9. Lin Dong
10. Yujing Bi
11. Guangwei Liu

• Curated by eLife

  Evaluation Summary:

  Wang and colleagues report that the expression of Piezo1 (an ion channel and mechanical sensor) is upregulated on dendritic cells (DC) under conditions of inflammation/high environmental stiffness resulting in DC activation, maturation, and skewing in DC functional polarity and metabolism. They show that Piezo1 knockout results in faster tumor progression and accumulation of more regulatory T cells, and that Smad3 and STAT4 are involved in DC-mediated differentiation of Th1 and Treg. Overall this represents a mechanistic advance in our understanding of DC biology as it relates to cancer and other human pathologies.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #3 agreed to share their name with the authors.)

Reviewed by eLife