Immunology

Cell circuits between leukemic cells and mesenchymal stem cells block lymphopoiesis by activating lymphotoxin beta receptor signaling

1. Xing Feng
2. Ruifeng Sun
3. Moonyoung Lee
4. Xinyue Chen
5. Shangqin Guo
6. Huimin Geng
7. Marcus Müschen
8. Jungmin Choi
9. Joao Pedro Pereira

• Curated by eLife

  eLife assessment

  This study describes a previously unrecognized positive feedback loop between leukemic cells and stromal cells impeding normal hematopoiesis mediated by lymphotoxin produced by cancer cells and its receptor expressed in stromal cells. These valuable findings will guide future research in both basic and clinical medicine. However, additional experimental evidence including more comparator groups would have further substantiated the authors' conclusions.

Reviewed by eLife

Transiently heritable fates and quorum sensing drive early IFN-I response dynamics

1. Laura C Van Eyndhoven
2. Vincent PG Verberne
3. Carlijn VC Bouten
4. Abhyudai Singh
5. Jurjen Tel

• Curated by eLife

  eLife assessment

  This study presents a valuable finding that adds to a growing body of evidence reporting heritable cell states that can guide fate choices in single cells, in this case the fate of early IFN-I response. The evidence supporting the claims of the authors is solid, although testing the generalizability of the result to other cell types or contexts and strengthening the link to epigenetic regulation would have strengthened the study. Overall, this work will be of interest to a wide set of scientists, including cell biologists, immunologists, and systems biologists.

Reviewed by eLife

Post-phagocytosis activation of NLRP3 inflammasome by two novel T6SS effectors

1. Hadar Cohen
2. Noam Baram
3. Chaya Mushka Fridman
4. Liat Edry-Botzer
5. Dor Salomon
6. Motti Gerlic

Reviewed by Review Commons

Antimicrobial peptides do not directly contribute to aging in Drosophila , but improve lifespan by preventing dysbiosis

1. M.A. Hanson
2. B. Lemaitre

Reviewed by Review Commons

Lineage tracing reveals fate bias and transcriptional memory in human B cells

1. Michael Swift
2. Felix Horns
3. Stephen R Quake

Reviewed by Review Commons

Senataxin and RNase H2 act redundantly to suppress genome instability during class switch recombination

1. Hongchang Zhao
2. Stella R Hartono
3. Kirtney Mae Flores de Vera
4. Zheyuan Yu
5. Krishni Satchi
6. Tracy Zhao
7. Roger Sciammas
8. Lionel Sanz
9. Frédéric Chédin
10. Jacqueline Barlow

• Curated by eLife

  eLife assessment

  This paper will be of interest to the audience in the fields of genome stability and B lymphocyte biology for highlighting the role of R loop metabolism in maintaining genome integrity during antigen gene diversification. Although RNA:DNA hybrids and R loops have been described at the immunoglobulin (Ig) loci long ago, their contribution to Ig heavy chain (Igh) class switch recombination and Igh locus integrity have not been fully elucidated yet. Overall, the experiments and results generally support this conclusion; however, several aspects of the model put forward are highly speculative in the current form.

Reviewed by eLife

Quantifying changes in the T cell receptor repertoire during thymic development

1. Francesco Camaglia
2. Arie Ryvkin
3. Erez Greenstein
4. Shlomit Reich-Zeliger
5. Benny Chain
6. Thierry Mora
7. Aleksandra M Walczak
8. Nir Friedman

• Curated by eLife

  eLife assessment

  This paper addresses an important question within adaptive immunity, namely whether the T cell receptor (TCR) repertoire of negatively selected thymocytes shares common features. The authors analyze T cell receptor sequences from mice as they progress through positive selection, CD4/CD8 lineage commitment, and negative selection, to find small but consistent differences between the repertoires at these selection stages. They argue that their findings do not indicate any sequence-specific selection; however, some of the conclusions drawn are currently incompletely supported by the performed analyses.

Reviewed by eLife

SARS-CoV-2-specific CD4+ and CD8+ T cell responses can originate from cross-reactive CMV-specific T cells

1. Cilia R Pothast
2. Romy C Dijkland
3. Melissa Thaler
4. Renate S Hagedoorn
5. Michel GD Kester
6. Anne K Wouters
7. Pieter S Hiemstra
8. Martijn J van Hemert
9. Stephanie Gras
10. JH Frederik Falkenburg
11. Mirjam HM Heemskerk

• Curated by eLife

  eLife assessment

  This is a very elegant study which clearly demonstrates the existence of CMV specific memory T cells in CMV+ pre-pandemic individuals that are capable of recognising epitopes from SARS-CoV-2. It provides new insights into the development of cross-reactive immune cells that was not anticipated. The study has been elegantly performed and presents important findings. In particular, the discovery of a public TCR which mediates the crossreactivity described is an important finding.

Reviewed by eLife

ARPC5 isoforms and their regulation by calcium-calmodulin-N-WASP drive distinct Arp2/3-dependent actin remodeling events in CD4 T cells

1. Lopamudra Sadhu
2. Nikolaos Tsopoulidis
3. Md Hasanuzzaman
4. Vibor Laketa
5. Michael Way
6. Oliver T Fackler

• Curated by eLife

  eLife assessment

  This fundamental study demonstrates that the two isoforms of the ARPC5 subunit (ARPC5 and ARPC5L) of the Arp2/3 complex have specific functions in regulating cytoplasmic and nuclear actin filament assembly in response to DNA replication stress and T cell receptor signaling in T lymphocytes. The data presented in the manuscript are convincing and of good technical quality, and the study provides interesting new insights into specific cellular roles of different Arp2/3 isoforms in T lymphocytes.

Reviewed by eLife

Longitudinal analysis of invariant natural killer T cell activation reveals a cMAF-associated transcriptional state of NKT10 cells

1. Harry Kane
2. Nelson M LaMarche
3. Áine Ní Scannail
4. Amanda E Garza
5. Hui-Fern Koay
6. Adiba I Azad
7. Britta Kunkemoeller
8. Brenneth Stevens
9. Michael B Brenner
10. Lydia Lynch

• Curated by eLife

  Evaluation Summary:

  The manuscript by Kane et al. described transcriptional profiles of various subsets of activated iNKT cells using longitudinal scRNA-Seq analysis. The finding that IL-10 producing iNKT cells have a cMAF-associated gene signature similar to Tr1 cells is novel. Overall, the data is well presented, however, functional consequences of some findings require further investigation.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife