Immunology

Contrasting roles for IKK regulated inflammatory signalling pathways for development and maintenance of type 1 and adaptive γδ T cells

1. Farjana Islam
2. Cayman Williams
3. Thea Hogan
4. Louise Webb
5. Ines Boal-Carvalho
6. Benedict Seddon

• Curated by eLife

  eLife Assessment

  This study provides valuable insights into the role of the NF-kB and IKK signaling pathways in γδ T cell development and survival, using robust genetic mouse models. While the research is methodologically sound, some conclusions require further evidence, with incomplete analyses, particularly regarding cell-intrinsic effects and mechanistic details. Overall, the findings are significant for immunologists interested in innate-like T cell biology and advancing the understanding of γδ T cell differentiation and maintenance.

Reviewed by eLife

Ex vivo and in vivo CRISPR/Cas9 screenings identify the roles of protein N-glycosylation in regulating T-cell activation and functions

1. Yu Hong
2. Xiaofang Si
3. Wenjing Liu
4. Xueying Mai
5. Yu Zhang

• Curated by eLife

  eLife Assessment

  This valuable work investigates the role of protein N-glycosylation in regulating T-cell activation and function and suggests that B4GALT1 is a potential target for tumor immunotherapy. The strength of evidence is solid, and further mechanistic validation could be provided.

Reviewed by eLife

HEB collaborates with TCR signaling to upregulate Id3 and enable γδT17 cell maturation in the fetal thymus

1. Johanna S Selvaratnam
2. Juliana Dutra Barbosa da Rocha
3. Vinothkumar Rajan
4. Helen Wang
5. Emily C Reddy
6. Jenny Jiahuan Liu
7. Miki S Gams
8. Cornelis Murre
9. David L Wiest
10. Cynthia J Guidos
11. Juan Carlos Zúñiga-Pflücker
12. Michele K Anderson

• Curated by eLife

  eLife Assessment

  The study provides important mechanistic insight into the transcriptional control of γδT17 development, elegantly demonstrating how HEB and Id3 act sequentially and cooperatively to regulate γδT17 cell specification and maturation. The study provides compelling evidence that advances the understanding of E-Id protein dynamics in thymic T cell specification. The work is comprehensive, technically rigorous, and conceptually clear, and will be of interest to immunologists, developmental biologists, and those studying the molecular underpinnings of physiological outcomes.

Reviewed by eLife

A major histocompatibility complex (MHC) class II molecule that binds the same viral pathogen peptide with both nonamer and decamer core sequences for presentation to T cells

1. Anastasia Goryanin
2. Atlanta G Cook
3. Shahriar Behboudi
4. Jim Kaufman
5. Samer Halabi

Reviewed by preLights

B cell expression of the enzyme PexRAP, an intermediary in ether lipid biosynthesis, promotes antibody responses and germinal center size

1. Sung Hoon Cho
2. Marissa A Jones
3. Kaylor Meyer
4. David M Anderson
5. Sergei Chetyrkin
6. M Wade Calcutt
7. Richard M Caprioli
8. Clay F Semenkovich
9. Mark R Boothby

• Curated by eLife

  eLife Assessment

  This study provides useful insights into the ways in which germinal center B cell metabolism, particularly lipid metabolism, affects cellular responses. The authors use sophisticated mouse models to convincingly demonstrate that ether lipids are relevant for B cell homeostasis and efficient humoral responses. The authors then conducted in vivo as well as in vitro experiments, thereby strengthening their conclusions.

Reviewed by eLife

Explosive cytotoxicity of ‘ruptoblasts’ bridges hormonal surveillance and immune defense

1. Chew Chai
2. Eliya Sultan
3. Souradeep R. Sarkar
4. Lihan Zhong
5. Dania Nanes Sarfati
6. Orly Gershoni-Yahalom
7. Christine Jacobs-Wagner
8. Hawa Racine Thiam
9. Benyamin Rosental
10. Bo Wang

Reviewed by preLights

RadD from Fusobacterium nucleatum Engages NKp46 to Promote Antitumor Cytotoxicity

1. Ahmed Rishiq
2. Johanna Galski
3. Reem Bsoul
4. Mingdong Liu
5. Rema Darawshe
6. Renate Lux
7. Gilad Bachrach
8. Ofer Mandelboim

• Curated by eLife

  eLife Assessment

  This useful study describes a mechanism of microbial modulation of anti-tumor immunity, which is of considerable interest in the field. However, the experimental supports for the key mechanistic claim, the interaction between RadD and NKp46, are not robust. Multiple experimental inconsistencies, especially in vivo, weaken the conclusions, making the strength of evidence incomplete. Additional controls, direct binding assays, and clarification of in vivo mechanistic relevance would strengthen the work.

Reviewed by eLife

Pre-Omicron Immunity Generates IgG⁺ but Not IgA⁺ Memory B Cells Reactive to Omicron Spike Protein

1. T Tsoleridis
2. I Singh
3. D Onion
4. JG Chappell
5. A Kelly
6. J Nightingale
7. AM Valdes
8. BJ Ollivere
9. KB Hoehn
10. RA Urbanowicz
11. JK Ball

Reviewed by PREreview

Lipopolysaccharide stimulates dynamic changes in B cell metabolism to promote proliferation

1. Dana MS Cheung
2. Momchil Razsolkov
3. Fabrizia Bonacina
4. Stephen Andrews
5. Megan Sumoreeah
6. Linda V Sinclair
7. Andrew JM Howden
8. J Simon C Arthur

• Curated by eLife

  eLife Assessment

  This work reveals metabolic pathways and molecular events mechanistically linked to B cell activation. Using an unbiased, comprehensive proteome profiling method and various functional validation approaches, this study generated convincing evidence suggesting a role for amino acid uptake, cholesterol accumulation, and protein prenylation in the proliferation, survival, and biogenesis of B cells stimulated with LPS and other activating stimuli. The significance of the findings is considered to be fundamental, in that they will advance our understanding of cell metabolism during B cell activation.

Reviewed by eLife

Layers of immunity: Deconstructing the Drosophila effector response

1. Faustine Ryckebusch
2. Yao Tian
3. Mylene Rapin
4. Fanny Schüpfer
5. Mark Austin Hanson
6. Bruno Lemaitre

• Curated by eLife

  eLife Assessment

  This work provides one of the first important attempts to look at Drosophila immune responses against bacterial, viral, and fungal pathogens in a way that combines the roles of four major arms in immunity (Imd signaling, Toll signaling, phagocytosis, and melanization) rather than studying them separately. The findings are compelling and the tools provided can be used as they are, or built upon, in various contexts.

Reviewed by eLife