Immunology

Single-cell profiling of the lung immune cells of diabetes-tuberculosis comorbidity reveals reduced type-II interferon and elevated Th17 responses

1. Shweta Chaudhary
2. Mothe Sravya
3. Falak Pahwa
4. Sureshkumar V
5. Prateek Singh
6. Shivam Chaturvedi
7. Debasisa Mohanty
8. Debasis Dash
9. Ranjan Kumar Nanda

• Curated by eLife

  eLife Assessment

  This study presents a valuable finding on the intersection between tuberculosis and diabetes and the impact on immune responses, notably T cell and myeloid cell responses. The single-cell data collected and analyzed are convincing and provide a rich dataset to develop a more detailed understanding of cellular responses during Mtb infection of diabetic mice. Some of the mechanistic claims are incomplete, as there are no experiments performed to clearly define a role for IL-16 or IL-17 in disease. Inclusion of analysis of human samples would have strengthened the conclusions in the paper for translational impact, as well as the inclusion of a DM group alone in addition to DM-TB vs TB in some of the experiments.

Reviewed by eLife

Phase transition of WTAP regulates m6A modification of interferon-stimulated genes

1. Sihui Cai
2. Jie Zhou
3. Xiaotong Luo
4. Chenqiu Zhang
5. Shouheng Jin
6. Jian Ren
7. Jun Cui

• Curated by eLife

  eLife Assessment

  This important study demonstrates that interferon beta stimulation induces WTAP transition from aggregates to liquid droplets, coordinating m6A modification of a subset of mRNAs that encode interferon-stimulated genes and restricting their expression. The evidence presented is solid, supported by microscopy, immunoprecipitations, m6A sequencing, and ChIP, to show that WTAP phosphorylation controls phase transition and its interaction with STAT1 and the methyltransferase complex.

Reviewed by eLife

Tissue-resident memory CD4+ T cells are sustained by site-specific levels of self-renewal and continuous replacement

1. Jodie Chandler
2. M Elise Bullock
3. Arpit C Swain
4. Cayman Williams
5. Christiaan H van Dorp
6. Benedict Seddon
7. Andrew J Yates

• Curated by eLife

  eLife Assessment

  This paper provides a compelling and rigorous quantitative analysis of the turnover and maintenance of CD4+ tissue-resident memory T cell clones, in the skin and the lamina propria. It provides a fundamental advance in our understanding of CD4 T cell regulation. Interestingly, in both tissues, maintenance involves an influx from progenitors on the time scale of months. The evidence that is based on fate mapping and mathematical inference is strong, although open questions on the interpretation of the Ki67-based fate mapping remain.

Reviewed by eLife

Human giant GTPase GVIN1 forms an antimicrobial coatomer around the intracellular bacterial pathogen Burkholderia thailandensis

1. Weilun Guo
2. Shruti S Apte
3. Mary S Dickinson
4. So Young Kim
5. Miriam Kutsch
6. Jörn Coers

• Curated by eLife

  eLife Assessment

  This is a fundamental discovery revealing two independent IFNγ-induced pathways that restrict bacterial motility: one GBP1-dependent and the other GVIN1-dependent. The findings are supported by compelling evidence. While the paper is already very strong, there are a few points that could be addressed editorially or through the addition of a few key experiments.

Reviewed by eLife

Interleukin 10 controls the balance between tolerance, pathogen elimination and immunopathology in birds

1. Dominique Meunier
2. Ricardo Corona-Torres
3. Kay Boulton
4. Zhiguang Wu
5. Maeve Ballantyne
6. Laura Glendinning
7. Anum Ali Ahmad
8. Dominika Borowska
9. Lorna Taylor
10. Lonneke Vervelde
11. Jorge del Pozo
12. Marili Vasilogianni
13. José Jaramillo-Ortiz
14. Gonzalo Sanchez-Arsuaga
15. Androniki Psifidi
16. Fiona Tomley
17. Kellie A. Watson
18. Michael J. McGrew
19. Mark P. Stevens
20. Damer P. Blake
21. David A. Hume

• Curated by eLife

  eLife Assessment

  Despite the conserved anti-inflammatory activity in birds, whether IL-10 also controls avian intestinal homeostasis remains unclear. Generating genetic knockouts, Meunier et al. firmly established that a complete lack of IL-10 strengthened immunity against enteric bacteria in chickens, while also aggravating infection-inflicted tissue damage upon parasite infection. The findings presented in this manuscript are valuable, and the strength of evidence is convincing; however, it is advised that the deficiencies and weaknesses pointed out by all the reviewers are meticulously addressed.

Reviewed by eLife

CARD8 inflammasome activation during HIV-1 cell-to-cell transmission

1. Jessie Kulsuptrakul
2. Michael Emerman
3. Patrick S Mitchell

• Curated by eLife

  eLife Assessment

  In this study, the authors investigated whether HIV-1 cell-to-cell transmission activates the CARD8 inflammasome in macrophages. The data convincingly support the idea that CARD8 is activated by the viral protease, promoting inflammation. The study's significance is further enhanced by including time-course analyses in primary T cells and macrophages and provides valuable insights into the role of CARD8 in HIV-induced inflammation.

Reviewed by eLife

The secreted Nimrod NimB1 negatively regulates early steps of apoptotic cell phagocytosis in Drosophila

1. Asya Dolgikh
2. Samuel Rommelaere
3. Aseel Ghanem
4. Bianca Petrignani
5. Mickael Poidevin
6. Estee Kurant
7. Bruno Lemaitre

Reviewed by Review Commons

TRPV1+ neurons promote cutaneous immunity against Schistosoma mansoni

1. Juan M. Inclan-Rico
2. Adriana Stephenson
3. Camila M. Napuri
4. Heather L. Rossi
5. Li-Yin Hung
6. Christopher F. Pastore
7. Wenqin Luo
8. De’Broski R. Herbert

Reviewed by Arcadia Science

The immunometabolic topography of tuberculosis granulomas governs cellular organization and bacterial control

1. Erin F. McCaffrey
2. Alea C. Delmastro
3. Isobel Fitzhugh
4. Jolene S. Ranek
5. Sarah Douglas
6. Joshua M. Peters
7. Christine Camacho Fullaway
8. Marc Bosse
9. Candace C. Liu
10. Craig Gillen
11. Noah F. Greenwald
12. Sarah Anzick
13. Craig Martens
14. Seth Winfree
15. Yunhao Bai
16. Cameron Sowers
17. Mako Goldston
18. Alex Kong
19. Potchara Boonrat
20. Carolyn L. Bigbee
21. Roopa Venugopalan
22. Pauline Maiello
23. Edwin Klein
24. Mark A. Rodgers
25. Charles A. Scanga
26. Philana Ling Lin
27. Denise Kirschner
28. Sarah Fortune
29. Bryan D. Bryson
30. J. Russell Butler
31. Joshua T. Mattila
32. JoAnne L. Flynn
33. Michael Angelo

Reviewed by Rapid Reviews Infectious Diseases, PREreview

Longitudinal analysis of drift in the circulating human antibody repertoire over four years

1. Collin Joyce
2. Benjamin Nemoz
3. Raiza Bastidas
4. Bryan Briney
5. Dennis R. Burton

Reviewed by PREreview