Immunology

G protein subunit Gγ13-mediated signaling pathway is critical to the inflammation resolution and functional recovery of severely injured lungs

1. Yi-Hong Li
2. Yi-Sen Yang
3. Yan-Bo Xue
4. Hao Lei
5. Sai-Sai Zhang
6. Junbin Qian
7. Yushi Yao
8. Ruhong Zhou
9. Liquan Huang

• Curated by eLife

  eLife assessment

  This, in principle, useful study suggests that the G-protein subunit Gng13 is required for limiting injury and inflammation following H1N1 influenza infection via anti-inflammatory effects from ectopic tuft cells. While support for Gng13 helping to limit influenza injury in the transgenic mouse models used here is solid, evidence for these effects being mediated by normal tuft cells remains incomplete, giving conflicting data from mice that lack tuft cells entirely.

Reviewed by eLife

An Evaluation of the Tumor Microenvironment through CALR, IL1R1, IFNB1, and IFNG to Assess Prognosis and Immunotherapy Response in Bladder Cancer Patients

1. Lilong Liu
2. Zhenghao Liu
3. Lei Fan
4. Zhipeng Yao
5. Junyi Hu
6. Yaxin Hou
7. Yang Li
8. Yuhong Ding
9. Yingchun Kuang
10. Ke Chen
11. Yi Hao
12. Zheng Liu

• Curated by eLife

  eLife assessment

  This study investigates the associations of four ICD-related genes in bladder cancer with increased immune cell infiltration and more prolonged survival. The study is valuable because it identifies a risk-scoring model, showing a correlation between high-risk scores based on four ICD-related genes and weak anti-tumour immune function. However, the evidence supporting the association of these genes and immunotherapy response is incomplete.

Reviewed by eLife

Deconstructing Complexity: A Computational Topology Approach to Trajectory Inference in the Human Thymus with tviblindi

1. Jan Stuchly
2. David Novak
3. Nadezda Brdickova
4. Petra Hadlova
5. Ahmad Iksi
6. Daniela Kuzilkova
7. Michael Svaton
8. George Alehandro Saad
9. Pablo Engel
10. Herve Luche
11. Ana E. Sousa
12. Afonso R. M. Almeida
13. Tomas Kalina

• Curated by eLife

  eLife assessment

  The authors present an algorithm and workflow for the inference of developmental trajectories from single-cell data, including a mathematical approach to increase computational efficiency. While such efforts are in principle useful, the absence of benchmarking against synthetic data and a wide range of different single-cell data sets make this study incomplete. Based on what is presented, one can neither ultimately judge if this will be an advance over previous work nor whether the approach will be of general applicability.

Reviewed by eLife

RAG1 and RAG2 non-core regions are implicated in leukemogenesis and off-target V(D)J recombination in BCR-ABL1-driven B-cell lineage lymphoblastic leukemia

1. Xiaozhuo Yu
2. Wen Zhou
3. Xiaodong Chen
4. Shunyu He
5. Mengting Qin
6. Meng Yuan
7. Yang Wang
8. Woodvine Otieno Odhiambo
9. Yinsha Miao
10. Yanhong Ji

• Curated by eLife

  eLife assessment

  Using a set of animal models, this valuable paper shows tumor suppressive function of the non-core regions of RAG1/2 recombinases. The conclusions are supported by solid evidence.

Reviewed by eLife

CD81+ fibroblasts, a unique subpopulation with accelerated cellular senescence, exaggerate inflammation and activate neutrophils via C3/C3aR1 axis in periodontitis

1. Liangliang Fu
2. Chenghu Yin
3. Qin Zhao
4. Shuling Guo
5. Wenjun Shao
6. Ting Xia
7. Quan Sun
8. Liangwen Chen
9. Min Wang
10. Haibin Xia

• Curated by eLife

  eLife assessment

  This useful study identifies a population of CD81-positive fibroblasts showing senescence signatures that can activate neutrophils through the C3/C3aR1 axis, hence contributing to the inflammatory response in periodontitis. Solid evidence, combining in vitro and in vivo analyses and mouse and human data, supports these findings. The work could be of interest to researchers working in the senescence and oral medicine fields

Reviewed by eLife

Thymic dendritic cell-derived IL-27p28 promotes the establishment of functional bias against IFN-γ production in newly generated CD4+ T cells through STAT1-related epigenetic mechanisms

1. Jie Zhang
2. Hui Tang
3. Haoming Wu
4. Xuewen Pang
5. Rong Jin
6. Yu Zhang

• Curated by eLife

  eLife assessment

  This study presents a useful reassessment of the potential role of dendritic cell-derived IL-27 p28 cytokine in the functional maturation of CD4+CD8- thymocytes, and CD4+ recent thymic emigrants. The evidence supporting the claims of the authors is solid and serves to reaffirm what has been previously described, with the overall advance in understanding the mechanism(s) responsible for the intrathymic functional programming of CD4+ T cells being limited.

Reviewed by eLife

Expression of modified FcγRI enables myeloid cells to elicit robust tumor-specific cytotoxicity

1. Leen Farhat-Younis
2. Manho Na
3. Amichai Zarfin
4. Aseel Khateeb
5. Nadine Santana-Magal
6. Alon Richter
7. Amit Gutwillig
8. Diana Rasoulouniriana
9. Annette Gleiberman
10. Lir Beck
11. Tamar Giger
12. Avraham Ashkenazi
13. Adi Barzel
14. Peleg Rider
15. Yaron Carmi

• Curated by eLife

  eLife assessment

  The findings are fundamental for understanding IgM signaling in myeloid cells. The work is compelling in its ability to manipulate and harness myeloid cells to further anti-tumor immunity.

Reviewed by eLife

Downregulation of Mirlet7 miRNA family promotes Tc17 differentiation and emphysema via de-repression of RORγt

1. Phillip A Erice
2. Xinyan Huang
3. Matthew J Seasock
4. Matthew J Robertson
5. Hui-Ying Tung
6. Melissa A Perez-Negron
7. Shivani L Lotlikar
8. David B Corry
9. Farrah Kheradmand
10. Antony Rodriguez

• Curated by eLife

  eLife assessment

  This important study indicates a significant role for individual let-7 miRNA clusters in regulating generation of Tc17 CD8 cells and emphysema severity in a mouse model. The authors provide convincing evidence for let-7-mediated repression of the transcription factor RORgt and consequent modulation of IL-17-producing CD8 T cells, with correlated data from human emphysema material, though some of the effective let-7 clusters remain to be tested for the ability to modulate disease. The findings, which substantially advance the understanding of roles that let-7 miRNA clusters play in modulating both T cell responses and emphysematous lung disease, will be of interest to T cell and lung disease researchers.

Reviewed by eLife

A modular platform to display multiple hemagglutinin subtypes on a single immunogen

1. Dana Thornlow Lamson
2. Faez Amokrane Nait Mohamed
3. Mya Vu
4. Daniel P. Maurer
5. Larance Ronsard
6. Daniel Lingwood
7. Aaron G. Schmidt

• Curated by eLife

  eLife assessment

  This valuable study showcases a novel and exciting vaccine platform but the evidence supporting the claims is incomplete. The work would benefit from robust statistical analysis of experimental groups with a larger number of individuals. There is also comparison to other existing vaccine platforms (such as the mosaic nanoparticle where hemagglutinin trimers are used).

Reviewed by eLife

Machine Learning Assisted Spectral Fingerprinting for Immune Cell Phenotyping

1. Aceer Nadeem
2. Sarah Lyons
3. Aidan Kindopp
4. Amanda Jamieson
5. Daniel Roxbury

Reviewed by Arcadia Science