Immunology

Diversity and functional specialization of oyster immune cells uncovered by integrative single-cell level investigations

1. Sebastien De La Forest Divonne
2. Juliette Pouzadoux
3. Oceane Romatif
4. Caroline Montagnani
5. Guillaume Mitta
6. Delphine Destoumieux-Garzón
7. Benjamin Gourbal
8. Guillaume M Charriere
9. Emmanuel Vignal

• Curated by eLife

  eLife Assessment

  The manuscript by de La Forest Divonne et al. offers an important and detailed exploration of the immune cells in the oyster Crassostrea gigas, by correlating distinct hemocyte morphotypes with specific single-cell transcriptional profiles. The evidence supporting the conclusion is convincing, deriving from the comprehensive dataset that not only captures unicellular diversity but also associates these cells with distinct immune roles, making it an invaluable resource for the broader research community.

Reviewed by eLife

T-follicular helper cell profiles differ by malaria antigen and for children compared to adults

1. Catherine Suzanne Forconi
2. Christina Nixon
3. Hannah W Wu
4. Boaz Odwar
5. Sunthorn Pond-Tor
6. John M Ong'echa
7. Jonathan D Kurtis
8. Ann M Moormann

• Curated by eLife

  eLife Assessment

  This descriptive study used multiparameter spectral flow cytometry and clustering analysis of a subset of CD4 T cells, termed circulating T follicular helper (cTfh), responding to Plasmodium falciparum antigens, PfSEA -1A and PfGARP. The results from this comprehensive study provide valuable information regarding differences in cTfh response profiles between children and adults living in malaria-endemic Kenya and thus offer a potential usefulness towards improving choices of antigen candidates for malaria vaccines. However, the analysis and interpretation of antigen-specific CD4 cTfh responses remain incomplete.

Reviewed by eLife

Temporal dynamics of viral fitness and the adaptive immune response in HCV infection

1. Melanie Rose Walker
2. Preston Leung
3. Elizabeth Keoshkerian
4. Mehdi R Pirozyan
5. Andrew Lloyd
6. Fabio Luciani
7. Rowena A Bull

• Curated by eLife

  eLife Assessment

  The authors examined the evolution of hepatitis C virus (HCV) in a cohort of 14 subjects with recent HCV infections. They showed that viral fitness declines as the virus mutates to escape the immune response and can rebound later in infection as HCV accumulates additional mutations. The study contributes to an important aspect of viral evolution. The combination of approaches contributes to a convincing study.

Reviewed by eLife

A host enzyme reduces metabolic dysfunction-associated steatotic liver disease (MASLD) by inactivating intestinal lipopolysaccharide

1. Zhiyan Wang
2. Nore Ojogun
3. Yiling Liu
4. Lu Gan
5. Zeling Xiao
6. Jintao Feng
7. Wei Jiang
8. Yeying Chen
9. Benkun Zou
10. ChengYun Yu
11. Changshun Li
12. Asha Ashuo
13. Xiaobo Li
14. Mingsheng Fu
15. Jian Wu
16. Yiwei Chu
17. Robert S Munford
18. Mingfang Lu

• Curated by eLife

  eLife Assessment

  This important study highlights the key role of the gut-liver axis mediated by LPS in causing hepatic steatosis. The authors provide solid evidence, in vivo, in vitro, and in silico, for the role of acyloxyacyl hydrolase in mediating this effect using KO mice subjected to MASD-inducing diets. The findings are significant for the liver research community and others interested in the gut-liver axis.

Reviewed by eLife

Anti-citrullinated protein antibodies arise during affinity maturation of germline antibodies to carbamylated proteins in rheumatoid arthritis

1. Marta Escarra-Senmarti
2. Michael Chungyoun
3. Dylan Ferris
4. Jeffrey J. Gray
5. Felipe Andrade

Reviewed by Arcadia Science

Nora virus proliferates in dividing intestinal stem cells and sensitizes flies to intestinal infection and oxidative stress

1. Adrien Franchet
2. Samantha Haller
3. Miriam Yamba
4. Vincent Barbier
5. Angelica Thomaz-Vieira
6. Vincent Leclerc
7. Stefanie Becker
8. Kwang-Zin Lee
9. Igor Orlov
10. Danièle Spehner
11. Laurent Daeffler
12. Dominique Ferrandon

• Curated by eLife

  eLife Assessment

  This important study shows that the Nora virus, a natural Drosophila pathogen that also persistently infects many laboratory fly stocks, infects intestinal stem cells (ISCs), leading to a shorter life span and increased sensitivity to intestinal infection with the Pseudomonas bacterium. The authors provide convincing data to support their conclusions. The paper provides new insights into virus-host interactions in the Drosophila gut and serves as a warning for scientists who use the fruit fly as a model to study gut physiology.

Reviewed by eLife

DHRS7 Integrates NADP ⁺ /NADPH Redox Sensing with Inflammatory Lipid Signalling via the Oxoeicosanoid Pathway

1. Yanan Ma
2. King Lam Hui
3. Yohannes A. Ambaw
4. Tobias C. Walther
5. Robert V. Farese
6. Miklos Lengyel
7. Zaza Gelashvili
8. Dajun Lu
9. Philipp Niethammer

Reviewed by PREreview

The C3-C3aR axis modulates trained immunity in alveolar macrophages

1. Alexander P Earhart
2. Rafael Aponte Alburquerque
3. Marick Starick
4. Aasritha Nallapu
5. Lorena Garnica
6. Ayse Naz Ozanturk
7. Rahul Kumar Maurya
8. Xiaobo Wu
9. Jeffrey A Haspel
10. Hrishikesh S Kulkarni

• Curated by eLife

  eLife Assessment

  This study explores how complement protein C3 and its signalling may modulate immune training in alveolar macrophages. The findings are an important contribution to the field of trained immunity. The data presented is mainly solid, but incomplete in parts.

Reviewed by eLife

ImPaqT - A Golden Gate-based Toolkit for Zebrafish Transgenesis

1. Saskia Hurst
2. Christiane Dimmler
3. Mark R Cronan

• Curated by eLife

  eLife Assessment

  This study introduces a useful toolkit for zebrafish transgenesis, significantly enhancing the flexibility and efficiency of transgene generation for immunological applications. The authors provide supporting evidence through well-designed experiments, demonstrating the toolkit's utility in generating diverse and functional transgenic lines. While the findings are solid, additional functional validation and broader comparisons to existing systems would strengthen the overall evidence base and ensure broader relevance to the zebrafish field, thereby increasing the significance of the study.

Reviewed by eLife

Sex‐dimorphic gene regulation in murine macrophages across niches

1. Cassandra J McGill
2. Olivia S White
3. Ryan J Lu
4. Nirmal K Sampathkumar
5. Bérénice A Benayoun

Reviewed by Review Commons