Immunology

Phospholipid Scramblase 1 (PLSCR1) Regulates Interferon-Lambda Receptor 1 (IFN-λR1) and IFN-λ Signaling in Influenza A Virus (IAV) Infection

1. Alina X Yang
2. Lisa Ramos-Rodriguez
3. Parand Sorkhdini
4. Dongqin Yang
5. Carmelissa Norbrun
6. Sonoor Majid
7. Yong Zhang
8. Michael J Holtzman
9. David F Boyd
10. Yang Zhou

• Curated by eLife

  eLife Assessment

  This valuable manuscript presents a potentially novel mechanism by which the phospholipid scramblase, PLSCR1, defends against influenza A virus infection. The paper was based on solid findings involving knockout and lung-specific over-expressing Plscr1 mice, airway tissue expression, and mechanistic studies to show Plscr1 enhances type III interferon-mediated viral clearance. The study is extensive and overall well performed.

Reviewed by eLife

Sex-dependent gastrointestinal colonization resistance to MRSA is microbiota and Th17 dependent

1. Alannah Lejeune
2. Chunyi Zhou
3. Defne Ercelen
4. Gregory Putzel
5. Xiaomin Yao
6. Alyson R Guy
7. Miranda Pawline
8. Magdalena Podkowik
9. Alejandro Pironti
10. Victor J Torres
11. Bo Shopsin
12. Ken Cadwell

• Curated by eLife

  eLife Assessment

  This fundamental study highlights potential mechanisms underlying the sex-dependent bias in susceptibility to gut colonization by Methicillin-resistant Staphylococcus aureus (MRSA). The evidence supporting the conclusion is compelling. The work will interest biologists who study intestinal infection and immunity.

Reviewed by eLife

Deciphering the preeclampsia-specific immune microenvironment and the role of pro-inflammatory macrophages at the maternal–fetal interface

1. Haiyi Fei
2. Xiaowen Lu
3. Zhan Shi
4. Xiu Liu
5. Cuiyu Yang
6. Xiaohong Zhu
7. Yuhan Lin
8. Ziqun Jiang
9. Jianmin Wang
10. Dong Huang
11. Liu Liu
12. Songying Zhang
13. Lingling Jiang

• Curated by eLife

  eLife Assessment

  This valuable study investigates the immune system's role in pre-eclampsia. The authors map the immune cell landscape of the human placenta and find an increase in macrophages and Th17 cells in patients with pre-eclampsia. Following mouse studies, the authors suggest that the IGF1-IGF1R pathway might play a role in how macrophages influence T cells, potentially driving the pathology of pre-eclampsia. There is convincing evidence in this study that will be of interest to immunologists and developmental biologists.

Reviewed by eLife

Clonal stochasticity in early NK cell response to mouse cytomegalovirus is generated by mature subsets of varying proliferative ability

1. Darren Wethington
2. Saeed Ahmad
3. Marc Potempa
4. Giuseppe Giuliani
5. Oscar A Aguilar
6. Maheshwor Poudel
7. Simon Grassmann
8. William Stewart
9. Nicholas M Adams
10. Joseph C Sun
11. Lewis L Lanier
12. Jayajit Das

• Curated by eLife

  eLife Assessment

  This study combines mathematical models and experimental data to analyse the emergence of heterogeneity within clonal NK cell responses during antigen-specific cell expansion. Although it comprises different experimental data and tests different theoretical hypotheses, the main claims remain incomplete and would benefit from the consideration of several previous findings about clonal immune responses and corresponding mathematical approaches. The study presents valuable findings with the potential to provide key insights about NK cell development if proposed claims could be confirmed by additional analyses.

Reviewed by eLife

Soluble immune mediators orchestrate protective in vitro granulomatous responses across Mycobacterium tuberculosis complex lineages

1. Ainhoa Arbués
2. Sarah Schmidiger
3. Miriam Reinhard
4. Sonia Borrell
5. Sebastien Gagneux
6. Damien Portevin

• Curated by eLife

  eLife Assessment

  This study describes the impact of mycobacterial genetic diversity on host-infection phenotypes by assessing the effect of different M. tuberculosis lineages on granulomatous inflammation using a 3D in vitro granuloma model. Despite being descriptive and showing mostly correlative relationships, the findings are useful and provide some solid support regarding the functional impact of M. tuberculosis's natural diversity on host-pathogen interactions. The study will interest researchers working on mycobacteria and motivate future studies to understand how genetic diversity influences virulence and immunity outcomes.

Reviewed by eLife

Immunoglobulin M regulates airway hyperresponsiveness independent of T helper 2 allergic inflammation

1. Sabelo Hadebe
2. Anca Flavia Savulescu
3. Jermaine Khumalo
4. Katelyn Jones
5. Sandisiwe Mangali
6. Nontobeko Mthembu
7. Fungai Musaigwa
8. Welcome Maepa
9. Hlumani Ndlovu
10. Amkele Ngomti
11. Martyna Scibiorek
12. Javan Okendo
13. Frank Brombacher

• Curated by eLife

  eLife Assessment

  Studying several allergens in different mouse strains, the authors assessed the role of IgM in airway inflammatory responses and show that IgM deficient mice have reduced airway hyperresponsiveness. Although the findings are useful and interesting and among others show the expression of a protein that regulates actin in smooth cells, the study remains incomplete as the data and analyses only partly support their primary claim.

Reviewed by eLife

Extracellular matrix phenotyping by imaging mass cytometry defines distinct cellular matrix environments associated with allergic airway inflammation

1. J E Parkinson
2. M Ghafoor
3. R J Dodd
4. H E Tompkins
5. M Fergie
6. M Rattray
7. J E Allen
8. T E Sutherland

Reviewed by Review Commons

Inhibiting NINJ1-dependent plasma membrane rupture protects against inflammasome-induced blood coagulation and inflammation

1. Jian Cui
2. Hua Li
3. Dien Ye
4. Guoying Zhang
5. Yan Zhang
6. Ling Yang
7. Martha MS Sim
8. Jeremy P Wood
9. Yinan Wei
10. Zhenyu Li
11. Congqing Wu

• Curated by eLife

  eLife Assessment

  The authors aim to elucidate the mechanism by which pyroptosis (through the formation of Gasdermin D (GSDMD) pores in the plasma membrane) contributes to increased release of procoagulant Tissue Factor-containing microvesicles. The data offers solid mechanistic insights as to the interplay between pyroptosis and microvesicle release with NINJ1. The study provides useful insights into the potential of targeting Ninj1 as a therapeutic strategy.

Reviewed by eLife

Discovery of a Potent and Selective Inhibitor of Human NLRP3 with a Novel Binding Modality and Mechanism of Action

1. Kevin Wilhelmsen
2. Aditi Deshpande
3. Sarah Tronnes
4. Maitriyee Mahanta
5. Matthew Banicki
6. Mary Cochran
7. Samantha Cowdin
8. Kristen Fortney
9. George Hartman
10. Robert Hughes
11. Rusty Montgomery
12. Claudia Portillo
13. Paul Rubin
14. Yan Wang
15. Shijun Yan
16. Barry A Morgan
17. Assem Duisembekova
18. Romane Riou
19. Michael Marleaux
20. Inga V. Hochheiser
21. Hannes Buthmann
22. Dominic Ferber
23. Wei Wang
24. Melanie Cranston
25. Chloe M. McKee
26. Thea Mawhinney
27. Emma McKay
28. Bénédicte F. Py
29. Matthias Geyer
30. Rebecca C. Coll

Reviewed by preLights

SIV-specific neutralizing antibody induction following selection of a PI3K drive-attenuated nef variant

1. Hiroyuki Yamamoto
2. Tetsuro Matano

• Curated by eLife

  eLife Assessment

  Yamamoto and Matano provide convincing evidence that a G63E/R CD8+ T-cell escape mutation in the accessory viral protein Nef promote the induction of neutralizing antibody (nAb) responses in rhesus macaques infected with SIVmac239, which is usually largely resistant to neutralization. Functional analyses support that this mutation specifically impairs Nef`s ability to stimulate PI3K/Akt/mTORC2 signalling. This important study suggests that the accessory viral protein Nef impairs B cell function and effective humoral immune responses and is of interest for researchers and physicians interested in HIV/AIDS and vaccine development.

Reviewed by eLife