Immunology

TCR transgenic clone selection guided by immune receptor analysis and single cell RNA expression of polyclonal responders

1. Nincy Debeuf
2. Sahine Lameire
3. Manon Vanheerswynghels
4. Julie Deckers
5. Caroline De Wolf
6. Wendy Toussaint
7. Rein Verbeke
8. Kevin Verstaen
9. Hamida Hammad
10. Stijn Vanhee
11. Bart N. Lambrecht

• Curated by eLife

  eLife assessment

  The paper illustrates a valuable approach to generating TCR transgenic mice specific for known epitopes. There is some solid evidence for the efficacy of this approach, although only limited evidence is provided that the TCR clone in question successfully recapitulates the functional features of the endogenous response to the same antigen, and the claim that this method is superior to more traditional clone selection methods is incompletely substantiated by the data presented.

Reviewed by eLife

The zinc transporter Slc30a1 in macrophages plays a protective role against Salmonella infection

1. Pinanong Na-Phatthalung
2. Shumin Sun
3. Enjun Xie
4. Jia Wang
5. Junxia Min
6. Fudi Wang

• Curated by eLife

  eLife assessment

  Work described in this manuscript reveals the importance of the zinc transporter SLC30A1 in the antimicrobial function of macrophages, specifically against Salmonella. Cell-targeted deletion of the zinc transporter increased susceptibility of mice to systemic infection with Salmonella, leading to decreases in several cell functions such as nos2 expression. The authors argue that zinc homeostasis promotes macrophage cell function that is not conductive to the intracellular proliferation of Salmonella. This study provides novel and supportive evidence for a new pathway in nutritional immunity.

Reviewed by eLife

Fine-tuning spatial-temporal dynamics and surface receptor expression support plasma cell-intrinsic longevity

1. Zhixin Jing
2. Phillip Galbo
3. Luis Ovando
4. Megan Demouth
5. Skylar Welte
6. Rosa Park
7. Kartik Chandran
8. Yinghao Wu
9. Thomas MacCarthy
10. Deyou Zheng
11. David Fooksman

• Curated by eLife

  eLife assessment

  Despite the importance of long-lived plasma cells (LLPCs), particularly for the infection and vaccination field, it is still unclear how they acquire their longevity. With a solid genetic approach, the authors demonstrate quite convincingly a requirement for chemokine/chemokine receptor-mediated interaction in LLPC longevity. The data are very valuable for the development of new types of vaccines.

Reviewed by eLife

Discovery of CD80 and CD86 as recent activation markers on regulatory T cells by protein-RNA single-cell analysis

1. Dominik Trzupek
2. Melanie Dunstan
3. Antony J. Cutler
4. Mercede Lee
5. Leila Godfrey
6. Lorna Jarvis
7. Daniel B. Rainbow
8. Dominik Aschenbrenner
9. Joanne L. Jones
10. Holm H. Uhlig
11. Linda S. Wicker
12. John A. Todd
13. Ricardo C. Ferreira

Reviewed by PREreview

Unraveling the Power of NAP-CNB’s Machine Learning-enhanced Tumor Neoantigen Prediction

1. Almudena Méndez-Pérez
2. Andrés M. Acosta-Moreno
3. Carlos Wert-Carvajal
4. Pilar Ballesteros-Cuartero
5. Rubén Sánchez-García
6. José R Macías
7. Rebeca Sanz-Pamplona
8. Ramon Alemany
9. Carlos Óscar S. Sorzano
10. Arrate Muñoz-Barrutia
11. Esteban Veiga

• Curated by eLife

  eLife assessment

  This important study assesses a novel in silico neoantigen prediction algorithm combined with in vivo validation to determine important parameters of neoantigen immunogenicity and tumor control. The strength of evidence is compelling. This study contributes to the field and will aid in the development of improved personalized cancer vaccines.

Reviewed by eLife

Uremic toxin indoxyl sulfate induces trained immunity via the AhR-dependent arachidonic acid pathway in end-stage renal disease (ESRD)

1. Hee Young Kim
2. Yeon Jun Kang
3. Dong Hyun Kim
4. Jiyeon Jang
5. Su Jeong Lee
6. Gwanghun Kim
7. Hee Byung Koh
8. Ye Eun Ko
9. Hyun Mu Shin
10. Hajeong Lee
11. Tae-Hyun Yoo
12. Won-Woo Lee

• Curated by eLife

  eLife assessment

  The authors expand the concept of a new layer to training immunity, which is currently being highlighted by several colleagues in the field. The work provides important hints to understand end-stage renal disease. Overall, the rational approach leads to experimental results that are solid.

Reviewed by eLife

The protective roles of Eugenol on type 1 diabetes mellitus through NRF2 mediated oxidative stress pathway

1. Yalan Jiang
2. Pingping He
3. Ke Sheng
4. Yongmiao Peng
5. Huilan Wu
6. Songwei Qian
7. Weiping Ji
8. Xiaoling Guo
9. Xiaoou Shan

• Curated by eLife

  eLife assessment

  This valuable study partially succeeds in providing evidence to support the therapeutic potential of the plant-derived compound eugenol for ameliorating symptoms associated with Type 1 Diabetes, identifying Nuclear factor E2 - related factor (Nrf2) as a mediator of the effects induced by eugenol. Although the study provides some interesting data, the evidence for the proposed mechanism is currently incomplete.

Reviewed by eLife

Spontaneous human CD8 T cell and autoimmune encephalomyelitis-induced CD4/CD8 T cell lesions in the brain and spinal cord of HLA-DRB1*15-positive multiple sclerosis humanized immune system mice

1. Irini Papazian
2. Maria Kourouvani
3. Anastasia Dagkonaki
4. Vasileios Gouzouasis
5. Lila Dimitrakopoulou
6. Nikolaos Markoglou
7. Fotis Badounas
8. Theodore Tselios
9. Maria Anagnostouli
10. Lesley Probert

• Curated by eLife

  eLife assessment

  The humanized model of EAE represents a valuable model in which to evaluate mechanisms that may drive EAE-like processes in vivo. The data are solid given the revisions and expansion of numbers of mice to yield more statistical rigor. This model will be used by the greater community studying EAE.

Reviewed by eLife

Downregulation of Semaphorin 4A in keratinocytes reflects the features of non-lesional psoriasis

1. Miki Kume
2. Hanako Koguchi-Yoshioka
3. Shuichi Nakai
4. Yutaka Matsumura
5. Atsushi Tanemura
6. Kazunori Yokoi
7. Shoichi Matsuda
8. Yuumi Nakamura
9. Naoya Otani
10. Mifue Taminato
11. Koichi Tomita
12. Tateki Kubo
13. Mari Wataya-Kaneda
14. Atsushi Kumanogoh
15. Manabu Fujimoto
16. Rei Watanabe

• Curated by eLife

  eLife assessment

  This important study implicates Sempharon 4a in both mice and humans as a key suppressor of psoriatic inflammation. The data are in parts incomplete in defining the precise functionally relevant cellular source and mechanism. Nonetheless, this study brings new insight into psoriasis pathogenesis and a potential new therapeutic target.

Reviewed by eLife

Feedback loop regulation between viperin and viral hemorrhagic septicemia virus through competing protein degradation pathways

1. Xiaobing Lu
2. Meisheng Yi
3. Zhe Hu
4. Taoran Yang
5. Wanwan Zhang
6. E. Neil G. Marsh
7. Kuntong Jia

Reviewed by preLights