Cell Biology

Mechanisms of cilia regeneration in Xenopus multiciliated epithelium in vivo

1. Venkatramanan G. Rao
2. Vignesharavind Subramanianbalachandar
3. Magdalena M. Magaj
4. Stefanie Redemann
5. Saurabh S. Kulkarni

Reviewed by Review Commons, Arcadia Science

Actin dynamics sustains spatial gradients of membrane tension in adherent cells

1. Juan Manuel García-Arcos
2. Amine Mehidi
3. Julissa Sánchez Velázquez
4. Pau Guillamat
5. Caterina Tomba
6. Laura Houzet
7. Laura Capolupo
8. Giovanni D’Angelo
9. Adai Colom
10. Elizabeth Hinde
11. Charlotte Aumeier
12. Aurélien Roux

Reviewed by preLights

Proteomic landscape of tunneling nanotubes reveals CD9 and CD81 tetraspanins as key regulators

1. Roberto Notario Manzano
2. Thibault Chaze
3. Eric Rubinstein
4. Esthel Penard
5. Mariette Matondo
6. Chiara Zurzolo
7. Christel Brou

• Curated by eLife

  eLife assessment

  Notario Manzano et al. offer a valuable first analysis of proteins within tunneling nanotubes (TNTs), membranous bridges connecting cells. This work distinguishes TNTs from extracellular vesicles, but further experimental and analytical tools are needed to refine the TNT proteome. Solid data supports a role for tetraspanins CD9 and CD81 in TNT function. The proposed model for CD9 and CD81 is over-interpreted and requires additional evidence for stronger support.

Reviewed by eLife

Ca ²⁺ binding to Esyt is required to modulate membrane contact site density in Drosophila photoreceptors

1. Vaisaly R Nath
2. Harini Krishnan
3. Shirish Mishra
4. Padinjat Raghu

Reviewed by Review Commons

Interferon-Induced PARP14-Mediated ADP-Ribosylation in p62 Bodies Requires an Active Ubiquitin-Proteasome System

1. Rameez Raja
2. Banhi Biswas
3. Rachy Abraham
4. Hongrui Liu
5. Che-Yuan Chang
6. Hien Vu
7. Anthony K. L. Leung

Reviewed by Review Commons

Identification of Glucosidase II that regulates STIM1 activation dynamics

1. Yangchun Du
2. Sisi Zheng
3. Feifan Wang
4. Jia Li
5. Xiaoman Yuan
6. Chao Xi
7. Yandong Zhou
8. Youjun Wang

Reviewed by PREreview

Impact of Maximal Overexpression of a Non-toxic Protein on Yeast Cell Physiology

1. Yuri Fujita
2. Shotaro Namba
3. Hisao Moriya

• Curated by eLife

  eLife assessment

  This convincing study advances our understanding of the physiological consequences of the strong overexpression of non-toxic proteins in baker's yeast. The findings suggest that a massive protein burden results in nitrogen starvation and a shift in metabolism likely regulated via the TORC1 pathway, as well as defects in ribosome biogenesis in the nucleolus. The study presents findings and tools that are important for the cell biology and protein homeostasis fields.

Reviewed by eLife

A systematic bi-genomic split-GFP assay illuminates the mitochondrial matrix proteome and protein targeting routes

1. Yury S Bykov
2. Solène Zuttion
3. Bruno Senger
4. Ofir Klein
5. Yeynit Asraf
6. Hadar Meyer
7. Hubert D Becker
8. Róża Kucharczyk
9. Maya Schuldiner

• Curated by eLife

  eLife assessment

  This study represents a valuable addition to the catalog of mitochondrial proteins. With the use of methodology based on the bi-genomic split-GFP technology, the authors generate convincing data, including dually localized proteins and topological information, under various growth conditions in yeast. The study represents a starting point for further functional and/or mechanistic studies on mitochondrial protein biogenesis.

Reviewed by eLife

The ORP9-ORP11 dimer promotes sphingomyelin synthesis

1. Birol Cabukusta
2. Shalom Borst Pauwels
3. Jimmy JLL Akkermans
4. Niek Blomberg
5. Aat A Mulder
6. Roman I Koning
7. Martin Giera
8. Jacques Neefjes

• Curated by eLife

  eLife assessment

  This valuable manuscript systematically addresses the role of intracellular lipid transfer proteins on cellular lipid levels. It provides convincing evidence on the role of ORP9 and ORP11 in sphingolipid metabolism at the Golgi complex. This article will be of broad interest to cell biologists interested in lipid metabolism and membrane biology.

Reviewed by eLife

Spatial proteomics of ER tubules reveals CLMN, an ER-actin tether at focal adhesions that promotes cell migration

1. Holly Merta
2. Tadamoto Isogai
3. Blessy Paul
4. Gaudenz Danuser
5. W Mike Henne

Reviewed by PREreview