Cell Biology

The RAB27A effector SYTL5 regulates mitophagy and mitochondrial metabolism

1. Ana Lapao
2. Lauren Sophie Johnson
3. Laura Trachsel-Moncho
4. Sakshi Singh
5. Matthew Yoke Wui Ng
6. Sigve Nakken
7. Eeva-Liisa Eskelinen
8. Anne Simonsen

• Curated by eLife

  eLife Assessment

  This study presents valuable insights into the role of two proteins, Rab27A and SYTL5, which control vesicle transport and delivery. While the data is clear, the overall evidence is somewhat incomplete. Strengthening the mechanistic aspect would enhance the study, making it of greater interest to cell biologists studying membrane trafficking and mitochondria.

Reviewed by eLife

A Commander-independent function of COMMD3 in endosomal trafficking

1. Galen T. Squiers
2. Chun Wan
3. James Gorder
4. Harrison Puscher
5. Jingshi Shen

• Curated by eLife

  eLife Assessment

  This important study explores the commander-independent function of COMMD3-Arf1 in endosomal recycling. The evidence supporting the authors' claims is solid; however, the inclusion of additional validation experiments and control conditions would have further strengthened the study. The findings will be of significant interest to cell biologists working on membrane trafficking.

Reviewed by eLife

Control of ciliary transcriptional programs during spermatogenesis by antagonistic transcription factors

1. Weihua Wang
2. Junqiao Xing
3. Xiqi Zhang
4. Hongni Liu
5. Xingyu Liu
6. Haochen Jiang
7. Cheng Xu
8. Xue Zhao
9. Zhangfeng Hu

• Curated by eLife

  eLife Assessment

  This valuable study presents data suggesting the critical roles of two ancient proteins, XAP5 and XAP5L, in regulating the transcriptional program of ciliogenesis during mouse spermatogenesis. The supporting data are solid, and this work will be of interest to biomedical researchers studying ciliogenesis and reproduction.

Reviewed by eLife

Multi-omics analyses and machine learning prediction of oviductal responses in the presence of gametes and embryos

1. Ryan M Finnerty
2. Daniel J Carulli
3. Akshata Hedge
4. Yanli Wang
5. Frimpong Boadu
6. Sarayut Winuthayanon
7. Jianlin Jack Cheng
8. Wipawee Winuthayanon

• Curated by eLife

  eLife Assessment

  This important study reports the transcriptomic and proteomic landscapes of the oviducts at four different preimplantation stages during natural fertilization, pseudopregnancy, and superovulation. The supporting data are convincing. This work will be of interest to reproductive biologists and clinicians practicing reproductive medicine.

Reviewed by eLife

Enalapril mitigates aging by targeting antioxidative genes via phosphorylated Smad1/5/9

1. Wencong Lyu
2. Haochen Wang
3. Zhehao Du
4. Ran Wei
5. Jianuo He
6. Fanju Meng
7. Jinlong Bi
8. Lijun Zhang
9. Chao Zhang
10. Yiting Guan
11. Wei Tao

• Curated by eLife

  eLife Assessment

  The manuscript presents valuable data suggesting that enalapril elevates pSmad1/5/9 signaling, reduces cellular senescence, and enhances physiological functions in aged mice. However, the in vivo evidence remains incomplete, as studies blocking pSmad1/5/9 or using NAC to negate enalapril's purported benefits are lacking, and no lifespan extension data are shown. While in vitro findings support Smad1/5/9 as a key mediator, additional experiments - including BMP receptor inhibition and comprehensive senescence markers - are necessary to validate its essential role in vivo. Overall, the study provides promising insights into enalapril's anti-senescence potential but requires further rigorous investigation to fully substantiate its mechanism and therapeutic impact.

Reviewed by eLife

Quantification of Cyclin-CDK dissociation constants in living cells using fluorescence cross-correlation spectroscopy with green and near-infrared fluorescent proteins

1. Aika Toyama
2. Yuhei Goto
3. Yuhei Yamauchi
4. Hironori Sugiyama
5. Yohei Kondo
6. Atsushi Mochizuki
7. Kazuhiro Aoki

Reviewed by Review Commons

Bridging the Gap in Cancer Cell Behavior Against Matrix Stiffening: Insights from a Trizonal Model

1. Mohammad E Torki
2. Fan Liu
3. Rongguang Xu
4. Yunfeng Chen
5. Jeffery Fredberg
6. Zi Chen

• Curated by eLife

  eLife Assessment

  This study presents a valuable nonlinear mathematical model that addresses how cell shape transitions in response to ECM stiffness. The evidence supporting the claims of the authors is solid, although additional work is required to improve the manuscript. For instance, the authors should improve the overall readability of the text and amend the experimental validation section. The work will be of interest to scientists working on a spectrum of fields including cell mechanics, cell behaviors, and cancer research.

Reviewed by eLife

Fis1 is required for the development of the dendritic mitochondrial network in pyramidal cortical neurons

1. Klaudia Strucinska
2. Parker Kneis
3. Travis Pennington
4. Katarzyna Cizio
5. Patrycja Szybowska
6. Abigail Morgan
7. Joshua Weertman
8. Tommy L Lewis

Reviewed by preLights

Identification of a sub-population of synovial mesenchymal stem cells with enhanced treatment efficacy in a rat model of Osteoarthritis

1. Nedaa Al-Jezani
2. Asmaa Affan
3. Catherine Leonard
4. Nabangshu Das
5. Luiz Gustavo Almeida
6. Daniel Young
7. Anand O Masson
8. Antoine Dufour
9. Paul Salo
10. Pam Railton
11. James N Powell
12. Roman J Krawetz

• Curated by eLife

  eLife Assessment

  This important study focused on characterizing clonally derived MSC populations from the synovium of normal and osteoarthritis (OA) patients, demonstrating their potential to regenerate cartilage in vivo. Although the strength of evidence is solid, further work is needed to fill the gaps in the CD47Hi cell characterization and the in vivo response assessment. The study will be of interest to scientists advancing MSC based regenerative medicine approaches.

Reviewed by eLife

SRS microscopy identifies inhibition of vitellogenesis as a mediator of lifespan extension by caloric restriction in C. elegans

1. Bowen Yang
2. Bryce Manifold
3. Wuji Han
4. Catherin DeSousa
5. Wanyi Zhu
6. Aaron Streets
7. Denis V. Titov

Reviewed by Arcadia Science