Cell Biology

Nucleus softens during herpesvirus infection

1. Aapo Tervonen
2. Visa Ruokolainen
3. Simon Leclerc
4. Katie Tieu
5. Sébastien Lyonnais
6. Henri Niskanen
7. Jian-Hua Chen
8. Alka Gupta
9. Minna U Kaikkonen
10. Carolyn A Larabell
11. Delphine Muriaux
12. Salla Mattola
13. Daniel E Conway
14. Teemu O Ihalainen
15. Vesa Aho
16. Maija Vihinen-Ranta

Reviewed by PREreview

Targeting ZNRF3 and RNF43 to Restore Regeneration and Reverse Metabolic Dysfunction-Associated Steatotic Liver Disease

1. Federico Di Tullio
2. Sue Bin Yang
3. Lida Yang
4. Bruno Cogliati
5. Shamsa Roshan
6. Kaiyuan Guo
7. Abigail Glezer
8. Jonathan Conrad
9. Siddarth Vinod Kumar
10. Joana Almeida
11. Haoyuan Li
12. Shahina Saeed
13. Marina Barcena-varela
14. Emily Bramel
15. Anthony Lozano
16. Lianyong Su
17. Derrick Zhao
18. Huong Pham
19. Fanglin Ma
20. Amaia Lujambio
21. Sai Ma
22. Yizhou Dong
23. Huiping Zhou
24. Tianliang Sun

Reviewed by PREreview

Orderly mitosis shapes interphase genome architecture

1. Krishnendu Guin
2. Adib Keikhosravi
3. Raj Chari
4. Gianluca Pegoraro
5. Tom Misteli

• Curated by eLife

  eLife Assessment

  This valuable study combines microscopy and CRISPR screening in two different cell lines to identify factors involved in global chromatin organization, using centromere clustering as a proxy. Follow-up cell cycle synchronisation studies confirm roles in centromere clustering in mitosis. However, incomplete characterisation of the cell lines used limits the interpretation of the findings. The study will interest researchers studying genome organisation in mitosis.

Reviewed by eLife

Spatial control of secretory vesicle targeting by the Ync13–Rga7– Rng10 complex during cytokinesis

1. Sha Zhang
2. Davinder Singh
3. Yi-Hua Zhu
4. Katherine J. Zhang
5. Alejandro Melero
6. Sophie G. Martin
7. Jian-Qiu Wu

Reviewed by Review Commons

Large-scale identification of plasma membrane repair proteins revealed spatiotemporal cellular responses to plasma membrane damage

1. Yuta Yamazaki
2. Keiko Kono

• Curated by eLife

  eLife Assessment

  This work provides an important resource identifying 72 proteins as novel candidates for plasma membrane and/or cell wall damage repair in budding yeast, and describes the temporal coordination of exocytosis and endocytosis during the repair process. The data are convincing; however, additional experimental validation will better support the claim that repair proteins shuttle between the bud tip and the damage site.

Reviewed by eLife

MCL1 may not mediate chemoresistance

1. Kylin A. Emhoff
2. Kunho Chung
3. Dongmei Zhang
4. Belinda Willard
5. Timothy Chan
6. Babal Kant Jha
7. Shaun R. Stauffer
8. Jesse A. Coker
9. Jan Joseph Melenhorst

Reviewed by preLights

Multiciliated cells adapt the mechanochemical Piezo1-Erk1/2-Yap1 cell proliferation axis to fine-tune centriole number

1. Vani Narayanan
2. Venkatramanan G Rao
3. Angelo Arrigo
4. Saurabh S Kulkarni

Reviewed by Review Commons

Septins function in exocytosis via physical interactions with the exocyst complex in fission yeast cytokinesis

1. Davinder Singh
2. Yajun Liu
3. Yi-Hua Zhu
4. Sha Zhang
5. Shelby M Naegele
6. Jian-Qiu Wu

• Curated by eLife

  eLife Assessment

  How secretion is regulated during cell division and how membrane trafficking factors cooperate with the cytoskeleton during cell division remain poorly understood. In this work the authors find protein-protein interactions and localization dependencies between the polymeric septin cytoskeleton and the exocyst complex, using fission yeast as a model organism and using alphafold 3 based structural predictions. The work provides a valuable body of new information that will be of great interest to the cell biology community. The evidence is solid and provides the authors and the community a framework to test if the identified interfaces reflect bona fide interaction sites in vivo and in vitro in future.

Reviewed by eLife

High-content high-resolution microscopy and deep learning-assisted analysis reveals host and bacterial heterogeneity during Shigella infection

1. Ana Teresa López-Jiménez
2. Dominik Brokatzky
3. Kamla Pillay
4. Tyrese Williams
5. Gizem Özbaykal Güler
6. Serge Mostowy

• Curated by eLife

  eLife Assessment

  This manuscript describes an AI-automated microscopy-based approach to characterize both bacterial and host cell responses associated with Shigella infection of epithelial cells. The methodology is compelling and should be helpful for investigators studying a variety of intracellular pathogens. The authors have acquired important findings regarding host and bacterial responses in the context of infection, which should be followed up with further mechanistic-based studies.

Reviewed by eLife

A tool to pulse-label yeast Nuclear Pore Complexes in imaging and biochemical experiments

1. Annemiek C Veldsink
2. Jonas S Fischer
3. Sophie Hell
4. Karsten Weis
5. Liesbeth M Veenhoff

• Curated by eLife

  eLife Assessment

  This valuable study introduces a non-perturbative pulse-labeling strategy for yeast nuclear pore complexes (NPCs), employing a nanobody-based approach in order to selectively capture Nup84-containing complexes for imaging and biochemical analysis. The data convincingly demonstrate that a short induction period (20 minutes to 1 hour) yields a strong and sustained signal, enabling affinity purification that faithfully recapitulates the endogenous Nup84 interactome. This tool offers a powerful framework for investigating NPC dynamics and associated interactomes through both imaging and biochemical assays.

Reviewed by eLife