Cell Biology

An extracellular vesicle targeting ligand that binds to Arc proteins and facilitates Arc transport in vivo

1. Peter H Lee
2. Michael Anaya
3. Mark S Ladinsky
4. Justin M Reitsma
5. Kai Zinn

• Curated by eLife

  eLife assessment

  Extracellular vesicles (EVs) are emerging as important mediators of cell-to-cell signaling. Lee et al show convincingly that Stranded at second (Sas), a Drosophila cell surface protein, is trafficked by and localized to Drosophila EVs. However, the data supporting interaction with dArc and whether Sas facilitates the intercellular transfer of dArc protein or mRNA is incomplete. Moreover, almost all experiments rely on gain-of-function and over-expression of Sas, thus the relevance to normal physiological signaling is unclear.

Reviewed by eLife

Histone deacetylase 6 inhibition promotes microtubule acetylation and facilitates autophagosome–lysosome fusion in dystrophin‐deficient mdx mice

1. Akanksha Agrawal
2. Erin L. Clayton
3. Courtney L. Cavazos
4. Benjamin A. Clayton
5. George G. Rodney

• Curated by eLife

  eLife assessment

  This important study pinpoints nitrite oxide synthase 2 activity and decreased microtubule acetylation as distinct regulators of altered autophagic flux that may contribute to pathogenesis in a mouse model of Duchenne muscular dystrophy. While most of the evidence to support these claims is convincing, the claim that autophagy is improved with increased microtubule acetylation is incompletely supported. This work may be of broad interest to muscle biologists and has translational potential for the treatment of Duchenne muscular dystrophy.

Reviewed by eLife

TANGO-Light - optogenetic control of transcriptional modulators

1. Alicja Przybyszewska-Podstawka
2. Joanna Kałafut
3. Jakub Czapiński
4. Thu Ha Ngo
5. Arkadiusz Czerwonka
6. Adolfo Rivero-Müller

Reviewed by Arcadia Science

Endocytosis blocks the vesicular secretion of exosome marker proteins

1. Yiwei Ai
2. Chenxu Guo
3. Marta Garcia-Contreras
4. Laura S. Sánchez B.
5. Andras Saftics
6. Oluwapelumi Shodubi
7. Shankar Raghunandan
8. Junhao Xu
9. Shang Jui Tsai
10. Yi Dong
11. Rong Li
12. Tijana Jovanovic-Talisman
13. Stephen J. Gould

Reviewed by Arcadia Science

Arp2/3 complex activity enables nuclear YAP for naïve pluripotency of human embryonic stem cells

1. Nathaniel Paul Meyer
2. Tania Singh
3. Matthew L Kutys
4. Todd G Nystul
5. Diane L Barber

Reviewed by Review Commons

The yeast endocytic early/sorting compartment exists as an independent sub-compartment within the trans-Golgi network

1. Junko Y Toshima
2. Ayana Tsukahara
3. Makoto Nagano
4. Takuro Tojima
5. Daria E Siekhaus
6. Akihiko Nakano
7. Jiro Toshima

• Curated by eLife

  eLife assessment

  In this study, the authors use high-speed and high-resolution imaging to investigate the role of the yeast syntaxin homolog Tlg2p in endocytic vesicle sorting. They obtain compelling data to show that the Tlg2p-residing compartment within the trans-Golgi network functions as an early/sorting compartment, where endocytic cargos are sorted to either the recycling pathway or the endo-lysosomal pathway. The authors also describe additional molecular details of this sorting process, and overall provide important insights into the mechanism of endocytic vesicle sorting in budding yeast.

Reviewed by eLife

Phosphorylation of tyrosine 90 in SH3 domain is a new regulatory switch controlling Src kinase

1. Lenka Koudelková
2. Markéta Pelantová
3. Zuzana Brůhová
4. Martin Sztacho
5. Vojtěch Pavlík
6. Dalibor Pánek
7. Jakub Gemperle
8. Pavel Talacko
9. Jan Brábek
10. Daniel Rösel

• Curated by eLife

  eLife assessment

  This manuscript explores the potential regulatory role of a previously unstudied phosphorylation site in the Src kinase SH3 domain. A mutant intended to mimic the phosphorylation of this site, Y90E, shows enhanced activity and transforming capacity, reduced mobility in the lipid bilayer, and a more open catalytic structure. In general, these findings are supported by compelling evidence. The paper will be of interest to biochemists and structural biologists studying new mechanisms that are capable of modulating the allosteric regulation of multi-domain protein kinases.

Reviewed by eLife

The Uso1 globular head interacts with SNAREs to maintain viability even in the absence of the coiled-coil domain

1. Ignacio Bravo-Plaza
2. Victor G Tagua
3. Herbert N Arst
4. Ana Alonso
5. Mario Pinar
6. Begoña Monterroso
7. Antonio Galindo
8. Miguel A Peñalva

• Curated by eLife

  eLife assessment

  This paper reports a detailed functional analysis of the Rab1 effector Uso1, and it provides a fundamental advance in our understanding of how ER-derived vesicles deliver their cargo. The authors provide compelling evidence that the key function of Uso1 is promoting SNARE complex formation rather than tethering vesicles as generally assumed. These insights will be of interest to cell and structural biologists who study membrane traffic.

Reviewed by eLife

Mitochondrial defects caused by PARL deficiency lead to arrested spermatogenesis and ferroptosis

1. Enrico Radaelli
2. Charles-Antoine Assenmacher
3. Jillian Verrelle
4. Esha Banerjee
5. Florence Manero
6. Salim Khiati
7. Anais Girona
8. Guillermo Lopez-Lluch
9. Placido Navas
10. Marco Spinazzi

• Curated by eLife

  eLife assessment

  This manuscript reports an important finding that spermatogenic defects in Parl KO mice, a genetic model for Leigh syndrome, may result from mitochondrial defects leading to ferroptosis. The finding, if confirmed, would be of great significance because male germ cell ferroptosis has not been well characterized. However, the criteria for determining male germ cell ferroptosis were vague, and the supporting data were inadequate.

Reviewed by eLife

Zebrafish Rif1 impacts zygotic genome activation, replication timing, and sex determination

1. Emily A. Masser
2. Tyler D. Noble
3. Joseph C. Siefert
4. Duane Goins
5. Courtney G. Sansam
6. Christopher L. Sansam

• Curated by eLife

  eLife assessment

  This paper reports on the role of RIF1 during early stages of zebrafish embryonic development, with the important finding that Rif1 seems to be required predominantly to establish the correct embryonic transcriptional program first, followed by a switch to a more replication-timing centered later function. The evidence is convincing, with the major strength being the elegant system and the possibility to also address the problem of the maternal pool of Rif1. A weakness is that the study remains descriptive and the presentation slightly disconnected, with limited mechanistic insight. The work will be of interest for researchers both in the transcription and the replication field, especially for scientists investigating the interplay between the two processes.

Reviewed by eLife