Cell Biology

Genome-scale requirements for dynein-based trafficking revealed by a high-content arrayed CRISPR screen

1. Chun Hao Wong
2. Steven W. Wingett
3. Chen Qian
4. J. Matthew Taliaferro
5. Douglas Ross-Thriepland
6. Simon L. Bullock

Reviewed by Review Commons

Galectin-9 interacts with Vamp-3 to regulate cytokine secretion in dendritic cells

1. Rui Santalla Méndez
2. Andrea Rodgers Furones
3. René Classens
4. Manon Haverdil
5. Marta Canela Capdevila
6. Anne van Duffelen
7. Cornelia G. Spruijt
8. Michiel Vermeulen
9. Martin ter Beest
10. Annemiek B. van Spriel
11. Laia Querol Cano

Reviewed by Review Commons

The ER membrane protein complex governs lysosomal turnover of a mitochondrial tail-anchored protein, BNIP3, to restrict mitophagy

1. Jose M Delgado
2. Logan Wallace Shepard
3. Sarah W Lamson
4. Samantha L Liu
5. Christopher J Shoemaker

Reviewed by Review Commons

Activation of Nedd4L Ubiquitin Ligase by FCHO2-generated Membrane Curvature

1. Yasuhisa Sakamoto
2. Akiyoshi Uezu
3. Koji Kikuchi
4. Shiro Suetsugu
5. Hiroyuki Nakanishi

Reviewed by Review Commons

Loss of Ptpmt1 limits mitochondrial utilization of carbohydrates and leads to muscle atrophy and heart failure in tissue-specific knockout mice

1. Hong Zheng
2. Qianjin Li
3. Shanhu Li
4. Zhiguo Li
5. Marco Brotto
6. Daiana Weiss
7. Domenick Prosdocimo
8. Chunhui Xu
9. Ashruth Reddy
10. Michelle Puchowicz
11. Xinyang Zhao
12. M Neale Weitzmann
13. Mukesh K Jain
14. Cheng-Kui Qu

• Curated by eLife

  eLife assessment

  This paper provides a useful set of data examining the role of PTPMT1, a mitochondria-based phosphatase, in mitochondrial fuel selection. The data were collected and analyzed using solid methodology and can be used as a starting point for further studies that build on the findings here.

Reviewed by eLife

Interaction between Teneurin-2 and microtubules via EB proteins provides a platform for GABAA receptor exocytosis

1. Sotaro Ichinose
2. Yoshihiro Susuki
3. Nobutake Hosoi
4. Ryosuke Kaneko
5. Mizuho Ebihara
6. Hirokazu Hirai
7. Hirohide Iwasaki

• Curated by eLife

  eLife assessment

  This potentially important paper investigates the mechanisms that contribute to building inhibitory synapses through differential protein release from microtubules. The experiments are generally designed well, but the evidence supporting the conclusions is incomplete. This manuscript will be of interest to neuroscientists and cell biologists interested in intracellular trafficking and synapse maturation.

Reviewed by eLife

FAM76B regulates NF-κB-mediated inflammatory pathway by influencing the translocation of hnRNPA2B1

1. Dongyang Wang
2. Xiaojing Zheng
3. Lihong Chai
4. Junli Zhao
5. Jiuling Zhu
6. Yanqing Li
7. Peiyan Yang
8. Qinwen Mao
9. Haibin Xia

• Curated by eLife

  eLife assessment

  The paper provides important insight into the function of FAM76B protein as a regulator of inflammation. The knockout/overexpression data are solid, however, the mechanism of regulation and the role of FAM76B in neurodegeneration is incomplete and requires additional experimentation. The work will be of interest to researchers studying inflammation, particularly neuroinflammation.

Reviewed by eLife

An extracellular vesicle targeting ligand that binds to Arc proteins and facilitates Arc transport in vivo

1. Peter H Lee
2. Michael Anaya
3. Mark S Ladinsky
4. Justin M Reitsma
5. Kai Zinn

• Curated by eLife

  eLife assessment

  Extracellular vesicles (EVs) are emerging as important mediators of cell-to-cell signaling. Lee et al show convincingly that Stranded at second (Sas), a Drosophila cell surface protein, is trafficked by and localized to Drosophila EVs. However, the data supporting interaction with dArc and whether Sas facilitates the intercellular transfer of dArc protein or mRNA is incomplete. Moreover, almost all experiments rely on gain-of-function and over-expression of Sas, thus the relevance to normal physiological signaling is unclear.

Reviewed by eLife

Histone deacetylase 6 inhibition promotes microtubule acetylation and facilitates autophagosome–lysosome fusion in dystrophin‐deficient mdx mice

1. Akanksha Agrawal
2. Erin L. Clayton
3. Courtney L. Cavazos
4. Benjamin A. Clayton
5. George G. Rodney

• Curated by eLife

  eLife assessment

  This important study pinpoints nitrite oxide synthase 2 activity and decreased microtubule acetylation as distinct regulators of altered autophagic flux that may contribute to pathogenesis in a mouse model of Duchenne muscular dystrophy. While most of the evidence to support these claims is convincing, the claim that autophagy is improved with increased microtubule acetylation is incompletely supported. This work may be of broad interest to muscle biologists and has translational potential for the treatment of Duchenne muscular dystrophy.

Reviewed by eLife

TANGO-Light - optogenetic control of transcriptional modulators

1. Alicja Przybyszewska-Podstawka
2. Joanna Kałafut
3. Jakub Czapiński
4. Thu Ha Ngo
5. Arkadiusz Czerwonka
6. Adolfo Rivero-Müller

Reviewed by Arcadia Science