Cell Biology

Adaptation of endothelial cells to microenvironment topographical cues through lysyl oxidase like-2-mediated basement membrane scaffolding

1. Marion F Marchand
2. Noémie Brassard-Jollive
3. Claire Leclech
4. Jorge Barrasa-Fano
5. Yoann Atlas
6. Claudia Umana-Diaz
7. Apeksha Shapeti
8. Corinne Ardidie-Robouant
9. Tristan Piolot
10. Sabrina Martin
11. Philippe Mailly
12. Christophe Guilluy
13. Abdul I Bakarat
14. Catherine Monnot
15. Hans Van Oosterwyck
16. Stéphane Germain
17. Laurent Muller

• Curated by eLife

  eLife Assessment

  This study presents important findings describing the early assembly of vascular basement membrane and how vascular cells switch from responding to cues provided by the external environment to those provided by self-assembled basement membrane. The evidence supporting the claims of the authors is convincing, with state-of-the-art microscopy and several different culture conditions examined. The work will be of interest to cell biologists studying the ECM, vascular development, as well as medical scientists focused on diseases that depend on vascular growth.

Reviewed by eLife

Noncanonical amino acid incorporation enables minimally disruptive labeling of stress granule and TDP-43 proteinopathy

1. Hao Chen
2. Haocheng Wang
3. Yuning Lu
4. Peng Chen
5. Zhongfan Zheng
6. Tao Zhang
7. Jiou Wang

• Curated by eLife

  eLife Assessment

  Amyotrophic lateral sclerosis (ALS) affects nerve cells in the brain and spinal cord. The authors' approach to use genetic code expansion to tag two ALS proteins associated with stress granules has value and should be useful in the ALS field. Parts of the work are well done, but there are concerns that the evidence is incomplete overall, and additional controls would strengthen the study.

Reviewed by eLife

Anti-resonance in developmental signaling regulates cell fate decisions

1. Samuel J Rosen
2. Olivier Witteveen
3. Naomi Baxter
4. Ryan S Lach
5. Erik Hopkins
6. Marianne Bauer
7. Maxwell Z Wilson

• Curated by eLife

  eLife Assessment

  This important work combines theoretical analysis with precise experimental perturbation to demonstrate a previously unappreciated quantitative characteristic of the Wnt signaling pathway, which is anti-resonance, or a suppression of pathway output at intermediate activation frequencies. This effect is demonstrated experimentally with compelling evidence from optogenetic stimulation in multiple cell types, alongside modeling results that corroborate the phenomenon. While the demonstration of this phenomenon has yet to be extended to fully physiological situations, its clear existence within optogenetically stimulated systems shows that it is likely a significant factor that contributes to the behavior of this central signaling pathway.

Reviewed by eLife

Persistent contacts between Climp63 and microtubules cause mitotic defects and nuclear fragmentation

1. Jelmi uit de Bos
2. Ulrike Kutay

• Curated by eLife

  eLife Assessment

  This important study reveals that mitotic release of an ER-microtubule tether is critical for normal mitotic progression. Manipulating CLIMP63 phosphorylation, the authors provide convincing evidence that persistent microtubule-ER contacts activate the spindle assembly checkpoint and, if mitosis is forced to proceed, drive severe micronucleation. While the study provides new mechanistic insights, some evidence is indirect, and additional experiments would further refine the model.

Reviewed by eLife

Intraflagellar transport-20 coiled-coil domain mediates the channelrhodopsins trafficking to the cilia in Chlamydomonas reinhardtii

1. Alka Kumari
2. Suneel Kateriya

Reviewed by Arcadia Science

Stress granules and protein aggregates reveal intracellular resource competition

1. Hannah E. Buchholz
2. Sean A. Martin
3. Jane E. Dorweiler
4. Derek C. Prosser
5. Emily M. Sontag
6. Anita L. Manogaran

Reviewed by Arcadia Science

Chemotherapy resistance due to epithelial-to-mesenchymal transition is caused by abnormal lipid metabolic balance

1. Atsushi Matsumoto
2. Akihito Inoko
3. Takuya Tanaka
4. Gen-Ichi Konishi
5. Waki Hosoda
6. Takahiro Kojima
7. Koji Ohnishi
8. Junichi Ikenouchi

• Curated by eLife

  eLife Assessment

  This paper presents the fundamental discovery that lipid metabolic imbalance induced by Snail, an EMT-related transcription factor, contributes to the acquisition of chemoresistance in cancer cells. The evidence, supported by a wide range of methods and adequate quantification, provides a convincing mechanistic explanation of how Snail drives ectopic expression of the cholesterol- and drug-efflux transporter ABCA1. This work, which introduces a novel therapeutic concept targeting invasive cancer, will be of broad interest to researchers in cancer biology, lipid metabolism, and cell biology.

Reviewed by eLife

Phenylhydrazone-based endoplasmic reticulum proteostasis regulator compounds with enhanced biological activity

1. Gabriel M Kline
2. Lisa Boinon
3. Adrian Guerrero
4. Sergei Kutseikin
5. Gabrielle Cruz
6. Marnie P Williams
7. Ryan J Paxman
8. William E Balch
9. Jeffery W Kelly
10. Tingwei Mu
11. R Luke Wiseman

• Curated by eLife

  eLife Assessment

  This study reports the important development and characterization of next-generation analogs of the molecule AA263, which was previously identified for its ability to promote adaptive ER proteostasis remodeling. The evidence supporting the conclusions is convincing, with rigorous assays used to benchmark the changes in potency and efficacy of the AA263 analogs as well as AA263 targets. The ability of AA263 analogs to restore the loss of function associated with disease-associated proteins prone to misfolding will be of interest to pharmacologists, chemical biologists, and cell biologists, as well as those working on protein misfolding disorders.

Reviewed by eLife

ROCK2 inhibition has a dual role in reducing ECM remodelling and cell growth, while impairing migration and invasion

1. Daniel A. Reed
2. Anna E. Howell
3. Nadia Kuepper
4. Alice M. H. Tran
5. Astrid Magenau
6. Deborah S. Barkauskas
7. Max Nobis
8. Cecilia R. Chambers
9. Victoria Lee
10. Lily M. Channon
11. Jessie Zhu
12. Shona Ritchie
13. Janett Stoehr
14. Kaitlin Wylie
15. Julia Chen
16. Denise Attwater
17. Kate Harvey
18. Sunny Z. Wu
19. Kate Saw
20. Ruth J. Lyons
21. Anaiis Zaratzian
22. Michael Tayao
23. Andrew Da Silva
24. David Gallego-Ortega
25. Anthony J. Gill
26. Thomas R. Cox
27. Brooke A. Pereira
28. Kendelle J. Murphy
29. Jennifer P. Morton
30. Elgene Lim
31. Alexander Swarbrick
32. Sandra O’Toole
33. Michael S. Samuel
34. C. Elizabeth Caldon
35. Alexandra Zanin-Zhorov
36. Paul Timpson
37. David Herrmann

Reviewed by preLights

Ceramide transfer protein regulates G-protein coupled phospholipase signalling in Drosophila photoreceptors

1. Shirish Mishra
2. Ali Khan
3. Vaisaly R Nath
4. Tejaswini Manoj
5. BG Thejaswini
6. Amruta Naik
7. Ujjaini Dasgupta
8. Padinjat Raghu

Reviewed by Review Commons