Cell Biology

ROCK2 inhibition has a dual role in reducing ECM remodelling and cell growth, while impairing migration and invasion

1. Daniel A. Reed
2. Anna E. Howell
3. Nadia Kuepper
4. Alice M. H. Tran
5. Astrid Magenau
6. Deborah S. Barkauskas
7. Max Nobis
8. Cecilia R. Chambers
9. Victoria Lee
10. Lily M. Channon
11. Jessie Zhu
12. Shona Ritchie
13. Janett Stoehr
14. Kaitlin Wylie
15. Julia Chen
16. Denise Attwater
17. Kate Harvey
18. Sunny Z. Wu
19. Kate Saw
20. Ruth J. Lyons
21. Anaiis Zaratzian
22. Michael Tayao
23. Andrew Da Silva
24. David Gallego-Ortega
25. Anthony J. Gill
26. Thomas R. Cox
27. Brooke A. Pereira
28. Kendelle J. Murphy
29. Jennifer P. Morton
30. Elgene Lim
31. Alexander Swarbrick
32. Sandra O’Toole
33. Michael S. Samuel
34. C. Elizabeth Caldon
35. Alexandra Zanin-Zhorov
36. Paul Timpson
37. David Herrmann

Reviewed by preLights

Ceramide transfer protein regulates G-protein coupled phospholipase signalling in Drosophila photoreceptors

1. Shirish Mishra
2. Ali Khan
3. Vaisaly R Nath
4. Tejaswini Manoj
5. BG Thejaswini
6. Amruta Naik
7. Ujjaini Dasgupta
8. Padinjat Raghu

Reviewed by Review Commons

Sterols regulate ciliary membrane dynamics and hedgehog signaling in health and disease

1. Antonin Lamazière
2. Lucie Kozlowski
3. Jesus Ayala-Sanmartin
4. Manal Farhat
5. Nabil Belaid
6. Alexandre Certain
7. Bertrand Isidor
8. Thomas Besnard
9. Germain Trugnan
10. Gregory J Pazour
11. Gaëtan Després
12. Céline Deseille
13. Sarah Moreno
14. Thibaut Eguether

Reviewed by Review Commons

Lipid bilayer thinning near a ubiquitin ligase selects ER membrane proteins for degradation

1. Rudolf Pisa
2. Tom A. Rapoport

Reviewed by preLights

Human dynein-dynactin is a fast processive motor in living cells

1. Vikash Verma
2. Patricia Wadsworth
3. Thomas J Maresca

• Curated by eLife

  eLife Assessment

  In this valuable technical report, Verma et al. provide convincing evidence that endogenously tagged dynein and dynactin form processive motor complexes that move along microtubules in living cells. Using quantitative fluorescence microscopy, they directly compare the stoichiometry and motility of these complexes to kinesin-1, revealing distinct transport behaviors and regulatory properties. This study offers key methodological and conceptual advance for understanding the dynamics of native motor proteins within the cellular environment and will be of interest to the cell biology community.

Reviewed by eLife

Overexpression of Ssd1 and calorie restriction extend yeast replicative lifespan by preventing deleterious age-dependent iron uptake

1. J Ignacio Gutierrez
2. Claudia Edgar
3. Jessica K Tyler

• Curated by eLife

  eLife Assessment

  This important study uses innovative microfluidics-based single-cell imaging to monitor replicative lifespan, protein localization, and intracellular iron levels in aging yeast cells. The evidence for the proposed role of Ssd1 and reduced nutrients for lifespan through limiting iron uptake is convincing, even though some mechanistic details remain unclear. This work will be of interest to cell biologists working on aging and iron metabolism.

Reviewed by eLife

A transcriptionally active lipid vesicle encloses the injected Chimalliviridae genome in early infection

1. Emily G. Armbruster
2. Phoolwanti Rani
3. Jina Lee
4. Niklas Klusch
5. Joshua Hutchings
6. Lizbeth Y. Hoffman
7. Hannah Buschkaemper
8. Eray Enustun
9. Benjamin A. Adler
10. Koe Inlow
11. Arica R. VanderWal
12. Madelynn Y. Hoffman
13. Daksh Daksh
14. Ann Aindow
15. Amar Deep
16. Zaida K. Rodriguez
17. Chase J. Morgan
18. Majid Ghassemian
19. Thomas G. Laughlin
20. Emeric Charles
21. Brady F. Cress
22. David F. Savage
23. Jennifer A. Doudna
24. Kit Pogliano
25. Kevin D. Corbett
26. Elizabeth Villa
27. Joe Pogliano

Reviewed by Rapid Reviews Infectious Diseases, Arcadia Science

Endosome-associated Rab GTPases control distinct aspects of neural circuit assembly

1. Katherine X Dong
2. Hui Ji
3. David J Luginbuhl
4. Liqun Luo
5. Colleen N McLaughlin

• Curated by eLife

  eLife Assessment

  Dong et al. present a valuable analysis of mutant phenotypes of the Rab GTPases Rab5, Rab7, and Rab11 in Drosophila second-order olfactory neuron development. This is a solid characterization and comparison of the different Rab mutants on projection neuron development, with clear differences for the three Rabs, and by inference for the early, late, and recycling endosomal functions executed by each.

Reviewed by eLife

CIP2A Mediates the Recruitment of the SLX4-MUS81-XPF Tri-Nuclease Complex in Mitosis and Protects Against Replication Stress

1. Alice Meroni
2. Annica Pellizzari
3. Andrea Hänel
4. Nathalie Varisco
5. Pascale Brasier-Lutz
6. Isabell Witzel
7. Alessandro A Sartori
8. Manuel Stucki

Reviewed by Review Commons

Branched actin polymerization drives invasive protrusion formation to promote myoblast fusion during mouse skeletal muscle regeneration

1. Yue Lu
2. Tezin Walji
3. Pratima Pandey
4. Chuanli Zhou
5. Christa W Habela
6. Scott B Snapper
7. Rong Li
8. Elizabeth H Chen

• Curated by eLife

  eLife Assessment

  This study presents a valuable finding regarding the role of Arp2/3 and the actin nucleators N-WASP and WAVE complexes in myoblast fusion. The data presented is convincing, and the work will be of interest to biologists studying skeletal muscle stem cell biology in the context of skeletal muscle regeneration.

Reviewed by eLife