Cell Biology

Ciliary length regulation by intraflagellar transport in zebrafish

1. Yi Sun
2. Zhe Chen
3. Minjun Jin
4. Haibo Xie
5. Chengtian Zhao

• Curated by eLife

  eLife Assessment

  The manuscript represents a valuable conceptual and technical contribution to our understanding of ciliogenesis and intraflagellar transport in vertebrates. Through a series of solid and technically superb live imaging experiments to directly visualize intraflagellar transport in various zebrafish ciliated tissues, the authors unveil the surprising breadth of intraflagellar transport speed among differing organs and link this to cell type-specific differences in cilia length and intraflagellar transport train size. This work will be of broad interest to researchers in numerous fields, including development, cell biology, and imaging.

Reviewed by eLife

Epigenetic deprogramming driven by disruption of CIZ1-RNA nuclear assemblies in early-stage breast cancers

1. Dawn Coverley

Reviewed by PREreview

Mechanical Centrosome Fracturing during Cell Navigation

1. Madeleine T. Schmitt
2. Mauricio J.A. Ruiz-Fernandez
3. Kasia Stefanowski
4. Janina Kroll
5. Robert Hauschild
6. Jack Merrin
7. Thomas Penz
8. Eva Kiermaier
9. Christoph Bock
10. Tobias Straub
11. Jörg Renkawitz

Reviewed by Arcadia Science

Quantitative Comparison of Monomeric StayGold Variants Using Protein Nanocages in Living Cells

1. Giulia Viola
2. Kyle A. Jacobs
3. Joël Lemière
4. Matthew L. Kutys
5. Torsten Wittmann

Reviewed by Arcadia Science

CellSeg3D: self-supervised 3D cell segmentation for light-sheet microscopy

1. Cyril Achard
2. Timokleia Kousi
3. Markus Frey
4. Maxime Vidal
5. Yves Paychère
6. Colin Hofmann
7. Asim Iqbal
8. Sebastien B. Hausmann
9. Stéphane Pagès
10. Mackenzie Weygandt Mathis

• Curated by eLife

  eLife assessment

  This work presents a valuable new approach for self-supervised segmentation for fluorescence microscopy data, which could eliminate time-consuming data labeling and speed up quantitative analysis. The experimental evidence supplied is currently incomplete as the comparison with other methods is only done on a single dataset, lacks common metrics, and could not be easily reproduced for other sample data listed in the manuscript.

Reviewed by eLife

Visualizing sarcomere and cellular dynamics in skeletal muscle to improve cell therapies

1. Judith Hüttemeister
2. Franziska Rudolph
3. Michael H. Radke
4. Claudia Fink
5. Dhana Friedrich
6. Stephan Preibisch
7. Martin Falcke
8. Eva Wagner
9. Stephan E. Lehnart
10. Michael Gotthardt

• Curated by eLife

  eLife Assessment

  This study offers valuable information on how titin derived from different nuclei within the syncytium is organized and integrated during skeletal muscle development and remodeling. The authors developed a novel mCherry titin knock-in mice with the fluorophore mCherry inserted into titin's Z-disk region to track the titin during cell fusion. The approach using mcherry adds to understanding of the role and localization of titin in controlling stiffness of striated muscles and fine tuning contraction. The results demonstrate that the integration of titin into the sarcomere is tightly regulated, with its unexpected mobility aiding in the uniform distribution of titin post-cell fusion. Although the experimental approach is convincing, the work is very qualitative in its approaches, and the data needs rigorous statistical analysis. There is a need for some clarification concerning numbers of animals and control groups. Future studies will need more rigorous data analysis and interpretation.

Reviewed by eLife

Endogenous oligomer formation underlies DVL2 condensates and promotes Wnt/β-catenin signaling

1. Senem Ntourmas
2. Martin Sachs
3. Petra Paclíková
4. Martina Brückner
5. Vítĕzslav Bryja
6. Jürgen Behrens
7. Dominic B Bernkopf

• Curated by eLife

  eLife Assessment

  This valuable study contributes to the understanding of phase separation in Dishevelled (DVL) proteins, by investigating the endogenous complexes of DVL2 using ultracentrifugation and contrasting them with DVL1 and DVL3 behaviour and the functional validation of the DVL2 intrinsically disordered regions mediating the protein condensate. The study includes a solid characterisation of several overexpression constructs, including in KO cells. However, investigations of the roles of the described DVL2 regions at the endogenous level remain to be carried out.

Reviewed by eLife

Brain-derived exosomal hemoglobin transfer contributes to neuronal mitochondrial homeostasis under hypoxia

1. Zhengming Tian
2. Feiyang Jin
3. Zirui Xu
4. Yakun Gu
5. Mengyuan Guo
6. Yuning Li
7. Qianqian Shao
8. Yingxia Liu
9. Hanjiang Luo
10. Yue Wang
11. Suyu Zhang
12. Chenlu Yang
13. Xin Liu
14. Xunming Ji
15. Jia Liu

• Curated by eLife

  eLife Assessment

  This valuable paper seeks to determine the role of endogenous CNS hemoglobin in protecting mitochondrial homeostasis in hypoxia. There is merit in the work, although it remains incomplete as there is a question as to the validity of the hypoxia model as relevant to CNS-specific ischemia/hypoxia that should be considered. In particular, a whole-body hypoxia model may liberate exosomes from other hypoxic organs, which should be addressed by the authors. Overall, this work has the potential to be of broad interest to the neuroscience and hypoxia communities.

Reviewed by eLife

Evidence of centromeric histone 3 chaperone involved in DNA damage repair pathway

1. Prakhar Agarwal
2. Santanu Kumar Ghosh

Reviewed by Review Commons

GDF2 and BMP10 coordinate liver cellular crosstalk to maintain liver health

1. Dianyuan Zhao
2. Ziwei Huang
3. Xiaoyu Li
4. Huan Wang
5. Qingwei Hou
6. Yuyao Wang
7. Fang Yan
8. Wenting Yang
9. Di Liu
10. Shaoqiong Yi
11. Chunguang Han
12. Yanan Hao
13. Li Tang

• Curated by eLife

  eLife Assessment

  This valuable study delineates the cellular contributions of BMP signaling in liver development and function. The findings are convincing, and the study employs state-of-the-art molecular, genetic, and cellular approaches to demonstrate that hepatic stellate cells play a central role in liver health by mediating cell-to-cell crosstalk via the production of specific BMP proteins. This study will be of interest to scientists interested in developmental biology and organ physiology.

Reviewed by eLife