Cell Biology

PP2A-B56 regulates Mid1 protein levels for proper cytokinesis in fission yeast

1. Madeline L. Chrupcala
2. James B. Moseley

Reviewed by Review Commons

Glutamine catabolism supports amino acid biosynthesis and suppresses the integrated stress response to promote photoreceptor survival

1. Moloy T Goswami
2. Eric Weh
3. Shubha Subramanya
4. Katherine M Weh
5. Hima Bindu Durumutla
6. Heather Hager
7. Nicholas Miller
8. Sraboni Chaudhury
9. Anthony Andren
10. Peter Sajjakulnukit
11. Li Zhang
12. Cagri G Besirli
13. Costas A Lyssiotis
14. Thomas J Wubben

• Curated by eLife

  eLife assessment

  Goswami and colleagues used rod-specific Gls1 (the gene encoding glutaminase 1) knockout mice to investigate the role of GLS1 in photoreceptor health when GLS1 was deleted from developing or adult photoreceptor cells. This study is important as it shows the critical role of glutamine catabolism in photoreceptor cell health using in vivo model systems. The evidence supporting the authors' claims is convincing. The current manuscript would further benefit from validating the evidence with additional supporting data from IND-cKO with tamoxifen induction at adult age, testing GLS1 activity to provide glutamate for synaptic transmission, and examining metabolic crosstalk between RPE and neural retinas.

Reviewed by eLife

Nuclear basket proteins regulate the distribution and mobility of nuclear pore complexes in budding yeast

1. Janka Zsok
2. Francois Simon
3. Göksu Bayrak
4. Luljeta Isaki
5. Nina Kerff
6. Yoana Kicheva
7. Amy Wolstenholme
8. Lucien E Weiss
9. Elisa Dultz

Reviewed by Review Commons

TMX5/TXNDC15, a natural trapping mutant of the PDI family is a client of the proteostatic factor ERp44

1. Tatiana Soldà
2. Carmela Galli
3. Concetta Guerra
4. Carolin Hoefner
5. Maurizio Molinari

Reviewed by Review Commons

Triglyceride metabolism controls inflammation and APOE4 -associated disease states in microglia

1. Roxan A. Stephenson
2. Kory R. Johnson
3. Linling Cheng
4. Linda G. Yang
5. Jessica T. Root
6. Jaanam Gopalakrishnan
7. Han-Yu Shih
8. Priyanka S. Narayan

Reviewed by preLights

Formation of multinucleated osteoclasts depends on an oxidized species of cell surface-associated La protein

1. Evgenia Leikina
2. Jarred M Whitlock
3. Kamran Melikov
4. Wendy Zhang
5. Michael P Bachmann
6. Leonid Chernomordik

• Curated by eLife

  eLife assessment

  This manuscript provides an important advance in our understanding of the molecular events that promote osteoclast fusion. Compelling data support the conclusion that an oxidized form of the ubiquitous protein La promotes osteoclast fusion following enrichment at the cell surface of osteoclast progenitors. These data improve our understanding of the processes that regulate bone resorption and will be of broad interest to researchers in the fields of cell biology and musculoskeletal physiology.

Reviewed by eLife

S-acylation of NLRP3 provides a nigericin sensitive gating mechanism that controls access to the Golgi

1. Daniel M Williams
2. Andrew A Peden

• Curated by eLife

  eLife assessment

  This important paper implicates S-acylation of Cys-130 in recruitment of the inflammasome receptor NLRP3 to the Golgi, and it provides convincing evidence that S-acylation plays a key role in response to the stress induced by nigericin treatment. While Cys-130 does seem to play a previously unappreciated role in membrane association of NLRP3, further work will be needed to clarify the details of the mechanism.

Reviewed by eLife

Time-resolved proximity proteomics uncovers a membrane tension-sensitive caveolin-1 interactome at the rear of migrating cells

1. Rossana Girardello
2. Eleanor Martin
3. Gunnar Dittmar
4. Alexander Ludwig

• Curated by eLife

  eLife assessment

  This important study uses convincing time-resolved proximity proteomics, validated with proximity ligation assays, to provide new insight into mechanical regulation of caveolin-1 complexes that form in migrating cells. Solid follow up experiments reveal a reciprocal relationship between mechanosensitive caveolae and RhoGTPase signalling in migrating cells, but evidence supporting a direct link between the newly identified factors with a specific caveolae subpopulation remains incomplete at this stage.

Reviewed by eLife

A MSTNDel73C mutation with FGF5 knockout sheep by CRISPR/Cas9 promotes skeletal muscle myofiber hyperplasia

1. Ming-Ming Chen
2. Yue Zhao
3. Kun Yu
4. Xue-Ling Xu
5. Xiao-Sheng Zhang
6. Jin-Long Zhang
7. Su-Jun Wu
8. Zhi-Mei Liu
9. Yi-Ming Yuan
10. Xiao-Fei Guo
11. Shi-Yu Qi
12. Guang Yi
13. Shu-Qi Wang
14. Huang-Xiang Li
15. Ao-Wu Wu
16. Guo-Shi Liu
17. Shou-Long Deng
18. Hong-Bing Han
19. Feng-Hua Lv
20. Di Lian
21. Zheng-Xing Lian

• Curated by eLife

  eLife assessment

  The authors present a useful analysis of the phenotype of sheep in which the muscle developmental regulator myostatin has been mutated in a FGF5 knockout background. The goal was to produce sheep with a "double-muscled" phenotype, yet the genetically engineered sheep exhibited meat with a smaller cross-sectional area and higher number of muscle fibers. The work extends the extensive body of knowledge already published in this area. The authors provide evidence using in vitro experiments that Fosl1 regulates myogenesis, but the strength of evidence relating to the muscle phenotype and underlying cellular and molecular mechanism remains incomplete.

Reviewed by eLife