Cell Biology

Defining the chromatin-associated protein landscapes on Trypanosoma brucei repetitive elements using synthetic TALE proteins

1. Roberta Carloni
2. Tadhg Devlin
3. Pin Tong
4. Christos Spanos
5. Tanya Auchynnikava
6. Juri Rappsilber
7. Keith R Matthews
8. Robin C Allshire

• Curated by eLife

  eLife Assessment

  This work significantly advances our understanding of chromatin organization within regions of repetitive sequences in the parasitic protozoan Trypanosoma brucei. Using cutting edge interdisciplinary tools, the authors provide compelling evidence for two discrete types of repetitive DNA element-associated proteins- one set involved in essential centromere function; and, the other involved in glycoprotein antigenic variation via homologous recombination. Thus, these fundamental findings have implications for this parasite's biology, and for therapeutic targeting in kinetoplastid diseases. This work will be exciting to those in the centromere/mitosis and parasite immunity fields.

  [Editors' note: this paper was reviewed by Review Commons.]

Reviewed by eLife, Review Commons

KANK2 at focal adhesion regulates their maintenance and dynamics, while at fibrillar adhesions it influences cell migration via microtubule-dependent mechanism

1. Nikolina Stojanović
2. Anja Rac
3. Marija Lončarić
4. Ana Tadijan
5. Mladen Paradžik
6. Marta Acman
7. Jonathan D. Humphries
8. Martin J. Humphries
9. Andreja Ambriović-Ristov

Reviewed by preLights

Histone methyltransferase DOT1L differentially affects the development of dendritic cell subsets

1. Rianne G. Bouma
2. Willem-Jan de Leeuw
3. Aru Z. Wang
4. Muddassir Malik
5. Joeke G.C. Stolwijk
6. Veronique A.L. Konijn
7. Anne Mensink
8. Natalie Proost
9. Maarten K. Nijen Twilhaar
10. Tibor van Welsem
11. Negisa Seyed Toutounchi
12. Alsya J. Affandi
13. Jip T. van Dinter
14. Fred van Leeuwen
15. Joke M.M. den Haan

Reviewed by Review Commons

Division Asymmetry Drives Cell Size Variability in Budding Yeast

1. Félix Proulx-Giraldeau
2. Xin Gao
3. Yagya Chadha
4. Jordan Xiao
5. Kurt M Schmoller
6. Jan M Skotheim
7. Paul Francois

• Curated by eLife

  eLife Assessment

  The presented findings are important for the field of cell-cycle control. They provide new insights into the origin of cell size variability in budding yeast. The strength of evidence is solid. However, the conclusions could be more strongly supported by additional analysis.

Reviewed by eLife, Arcadia Science

Lysosome-Dependent Sphingolipid Regulation as a potential therapeutic Target for Cohen Syndrome

1. Fabrizio Vacca
2. Renuka Prasad
3. Huda Barakullah
4. Romain Da Costa
5. Stefania Vossio
6. Dimitri Moreau
7. Woong Sun
8. Howard Riezman
9. Muhammad Ansar

Reviewed by Review Commons

Cdk1 and PP2A constitute a molecular switch controlling orderly degradation of atypical E2Fs

1. Sapir Nachum-Raines
2. Noy Gamliel
3. Danit Wasserman
4. Nasrin Qassem
5. Inbal Sher
6. Julia Guez-Haddad
7. Michael J Emanuele
8. Jordan H Chill
9. Amit Tzur

Reviewed by Review Commons

Efficiency and localisation of AURKA degradation by PROTACs is modulated by deubiquitinases UCHL5 and target-selective OTUD6A

1. Annabel Cardno
2. Karen Roberts
3. Catherine Lindon

• Curated by eLife

  eLife Assessment

  This study describes a genetic screen to identify deubiquitinases (DUBs) that counteract the activity of small molecule degraders (PROTACs). The presented data is valuable, identifying OTUD6A and UCHL5 as DUBs that impact the efficacy and potency of PROTAC-mediated degradation in distinct subcellular compartments. While the conclusions are broadly supported and the methods employed are solid, the validation of OTUD6A and UCHL5 mechanisms requires additional study. Overall, these findings merit further evaluation by the targeted protein degradation community when developing and optimizing PROTACs and efforts to achieve compartment-specific degradation.

Reviewed by eLife

Centrosome Migration and Apical Membrane Formation in Polarized Epithelial Cells: Insights from the MDCK Cyst Model

1. Po-Kai Wang
2. Keng-Hui Lin
3. Tang K Tang

• Curated by eLife

  eLife Assessment

  This important study contributes to our understanding of how epithelial cells establish polarity by identifying a hierarchy in which Par3 acts upstream of centrosome positioning and apical membrane initiation. The evidence supporting the main conclusions is convincing, although several aspects of the model remain only partially supported due to unresolved questions about microtubule organization and the need for clearer integration of quantitative and conceptual points raised in review. The work will be of interest to cell and developmental biologists, but the conclusions would be strengthened by greater precision in methodology, terminology, and interpretation.

Reviewed by eLife

A conserved Hsp70 phosphorylation regulates cell cycle progression after DNA damage

1. Thomas Moss
2. Alexandra Wooldredge
3. Koustav Bhakta
4. Matthew Cronin
5. Jason E Gestwicki
6. Shaeri Mukherjee

• Curated by eLife

  eLife Assessment

  This potentially valuable manuscript focuses on the phosphorylation of residue T495 as a mechanism to inactivate HSP70 and disrupt cell cycle progression in response to DNA damage. The evidence supporting this model is incomplete and would be strengthened by additional studies defining the extent of T495 phosphorylation induced by DNA damage, identifying the kinase responsible for phosphorylating T495 of HSP70, and further elucidation of the functional implications of T495 phosphorylation in human cells. This work will be of interest to scientists focused on topics including chaperone biology, proteostasis, cell cycle progression, and DNA damage.

Reviewed by eLife, preLights

PIP5K-Ras bistability triggers plasma membrane symmetry breaking to define cellular polarity and regulate migration

1. Yu Deng
2. Tatsat Banerjee
3. Satomi Matsuoka
4. Debojyoti Biswas
5. Liz A. Kurtz
6. Jane Borleis
7. Yu Long
8. Parijat Banerjee
9. Huiwang Zhan
10. Dhiman Sankar Pal
11. Nathan H. Roy
12. Masahiro Ueda
13. Pablo A. Iglesias
14. Peter N. Devreotes

Reviewed by preLights