Cell Biology

Analysis of cancer mutations introduced into the Drosophila Notch Negative Regulatory Region uncovers a diversity of regulatory outcomes

1. Hideyuki Shimizu
2. Martin Baron

• Curated by eLife

  eLife Assessment

  This important study presents a technically rigorous and carefully controlled analysis of the signalling potential of cancer-associated gain-of-function Notch alleles. The work is clearly presented, and the experiments are robust, comprehensive, and well-controlled. While some data primarily establish the system or report negative findings, the comparative approach in a well-characterized model provides convincing mechanistic evidence for how these Notch variants function. This study will be of interest to researchers in both developmental and cancer biology.

Reviewed by eLife

A green lifetime biosensor for calcium that remains bright over its full dynamic range

1. Franka H van der Linden
2. Stephen C Thornquist
3. Rick M ter Beek
4. Jelle Y Huijts
5. Mark A Hink
6. Theodorus W J Gadella
7. Gaby Maimon
8. Joachim Goedhart

• Curated by eLife

  eLife Assessment

  This manuscript reports on an FLIM-based calcium biosensor, G-CaFLITS. It represents an important contribution to the field of genetically-encoded fluorescent biosensors, and will serve as a practical tool for the FLIM imaging community. The paper provides convincing evidence of G-CaFLITS's photophysical properties and its advantages over previous biosensors such as Tq-Ca-FLITS. Although the benefits of G-Ca-FLITS over Tq-Ca-FLITS are limited by the relatively small wavelength shift, it presents some advantages in terms of compatibility with available instrumentation and brightness consistency.

Reviewed by eLife

NudC moonlights in ribosome biogenesis and homeostasis in Drosophila melanogaster polyploid cells

1. Duoduo Shi
2. Yuko Shimada-Niwa
3. Naoki Okamoto
4. Akira Nakamura
5. Yuya Ohhara
6. Wei Sun
7. Ryusuke Niwa

Reviewed by Review Commons

Ciliary and non-ciliary functions of CEP104 in Xenopus

1. Panagiota Louka
2. Louiza Potamiti
3. Mihalis I. Panayiotidis
4. Paris Skourides

Reviewed by Review Commons

A systematic bi-genomic split-GFP assay illuminates the mitochondrial matrix proteome and protein targeting routes

1. Yury S Bykov
2. Solene Zuttion
3. Dunya Edilbi
4. Marina Polozova
5. Johanna Arnold
6. Sergey Malitsky
7. Maxim Itkin
8. Bruno Senger
9. Ofir Klein
10. Yeynit Asraf
11. Hadar Meyer
12. Hubert D Becker
13. Roza Kucharczyk
14. Maya Schuldiner

• Curated by eLife

  eLife Assessment

  This study represents a valuable addition to the catalog of mitochondrial proteins. With the use of methodology based on the bi-genomic split-GFP technology, the authors generate convincing data, including dually localized proteins and topological information, under various growth conditions in yeast. The study represents a key basis for further functional and/or mechanistic studies on mitochondrial protein biogenesis.

Reviewed by eLife

Apelin-13 alleviate inflammatory reaction of ischemia reperfusion in rat kidney transplantation via NF-kappa B signaling pathway

1. Irene Capelli
2. Valeria Aiello
3. Lorenzo Gasperoni
4. Gaetano La Manna

Reviewed by PREreview

HAK-actin, U-ExM-compatible probe to image the actin cytoskeleton

1. Olivier Mercey
2. Luc Reymond
3. Florent Lemaître
4. Isabelle Mean
5. Marine H. Laporte
6. Marine Olivetta
7. Karin Sadoul
8. Omaya Dudin
9. Virginie Hamel
10. Paul Guichard

Reviewed by preLights

Condensin II collaborates with cohesin to establish and maintain interphase chromosome territories

1. Takao Ono
2. Masatoshi Takagi
3. Hideyuki Tanabe
4. Tomoko Fujita
5. Noriko Saitoh
6. Akatsuki Kimura
7. Tatsuya Hirano

Reviewed by Review Commons

The Anti-Inflammatory Role of GPNMB in Post-Traumatic Osteoarthritis

1. Asaad A Al-Adlaan
2. Bryson Cook
3. Nazar J Hussein
4. Fatima A Jaber
5. Trinity Kronk
6. Ernesto Solorzano Z
7. Salvatore Frangiamore
8. Hope C Ball
9. Fayez F Safadi

• Curated by eLife

  eLife Assessment

  This study demonstrates the cartilage-protective effects of osteoactivin in inflammatory experimental models. The work offers valuable insights advancing current knowledge regarding regulation of joint inflammation and tissue degeneration. The evidence provided is compelling and suggests that osteoactivin may serve as a promising therapeutic target for inflammatory joint diseases.

Reviewed by eLife

Acidocalcisome-like vacuoles constitute a feedback-controlled phosphate buffering system for the cytosol

1. Samuel Bru
2. Lydie Michaillat Mayer
3. Geun-Don Kim
4. Danye Qiu
5. Henning J Jessen
6. Andreas Mayer

• Curated by eLife

  eLife Assessment

  This study provides fundamental insights into eukaryotic phosphate homeostasis by demonstrating how yeast vacuoles dynamically regulate cytosolic phosphate levels. The conclusions are convincing, supported by an elegant combination of in vitro assays and in vivo measurements. This study will be of interest to cell biologists, particularly for those who are working in the field of phosphate metabolism.

Reviewed by eLife