Cell Biology

Lysosome-Dependent Sphingolipid Regulation as a potential therapeutic Target for Cohen Syndrome

1. Fabrizio Vacca
2. Renuka Prasad
3. Huda Barakullah
4. Romain Da Costa
5. Stefania Vossio
6. Dimitri Moreau
7. Woong Sun
8. Howard Riezman
9. Muhammad Ansar

Reviewed by Review Commons

Cdk1 and PP2A constitute a molecular switch controlling orderly degradation of atypical E2Fs

1. Sapir Nachum-Raines
2. Noy Gamliel
3. Danit Wasserman
4. Nasrin Qassem
5. Inbal Sher
6. Julia Guez-Haddad
7. Michael J Emanuele
8. Jordan H Chill
9. Amit Tzur

Reviewed by Review Commons

Efficiency and localisation of AURKA degradation by PROTACs is modulated by deubiquitinases UCHL5 and target-selective OTUD6A

1. Annabel Cardno
2. Karen Roberts
3. Catherine Lindon

• Curated by eLife

  eLife Assessment

  This study describes a genetic screen to identify deubiquitinases (DUBs) that counteract the activity of small molecule degraders (PROTACs). The presented data is valuable, identifying OTUD6A and UCHL5 as DUBs that impact the efficacy and potency of PROTAC-mediated degradation in distinct subcellular compartments. While the conclusions are broadly supported and the methods employed are solid, the validation of OTUD6A and UCHL5 mechanisms requires additional study. Overall, these findings merit further evaluation by the targeted protein degradation community when developing and optimizing PROTACs and efforts to achieve compartment-specific degradation.

Reviewed by eLife

Centrosome Migration and Apical Membrane Formation in Polarized Epithelial Cells: Insights from the MDCK Cyst Model

1. Po-Kai Wang
2. Keng-Hui Lin
3. Tang K Tang

• Curated by eLife

  eLife Assessment

  This important study contributes to our understanding of how epithelial cells establish polarity by identifying a hierarchy in which Par3 acts upstream of centrosome positioning and apical membrane initiation. The evidence supporting the main conclusions is convincing, although several aspects of the model remain only partially supported due to unresolved questions about microtubule organization and the need for clearer integration of quantitative and conceptual points raised in review. The work will be of interest to cell and developmental biologists, but the conclusions would be strengthened by greater precision in methodology, terminology, and interpretation.

Reviewed by eLife

PIP5K-Ras bistability triggers plasma membrane symmetry breaking to define cellular polarity and regulate migration

1. Yu Deng
2. Tatsat Banerjee
3. Satomi Matsuoka
4. Debojyoti Biswas
5. Liz A. Kurtz
6. Jane Borleis
7. Yu Long
8. Parijat Banerjee
9. Huiwang Zhan
10. Dhiman Sankar Pal
11. Nathan H. Roy
12. Masahiro Ueda
13. Pablo A. Iglesias
14. Peter N. Devreotes

Reviewed by preLights

VAMP8 function reveals tight linkage between endocytic recycling and endocytosis

1. Ailing Liu
2. Yueping Li
3. Zheng Huang
4. Wen Chen
5. Peiliu Xu
6. Xiangying Wei
7. Guosheng Hu
8. Shuangquan Liu
9. Xiaoxia Liu
10. Yaohui He
11. Danling Wang
12. Sandra L. Schmid
13. Zhiming Chen

Reviewed by Review Commons

Ubiquitination regulates granulostasis and DRiP accumulation in SGs under heat stress via the E3 ligase MKRN2

1. Emmanuel Amzallag
2. Yehuda M. Danino
3. Valentina Secco
4. Serena Carra
5. Eran Hornstein

Reviewed by Review Commons

Enteropathogenic E. coli-mediated Fast and Coordinated Ca²⁺ responses regulate NF-κB activation

1. Fangrui Guo
2. Roberto Ornelas Guevara
3. Linda Oussaedine
4. Geneviève Dupont
5. Laurent Combettes
6. Guy Tran Van Nhieu

• Curated by eLife

  eLife Assessment

  This study reports important advances in our understanding of how enteropathogenic E. coli (EPEC) interacts at the intestinal interface. Solid data describe a novel model of spatially coordinated calcium signaling to modulate NF-kB activation; additional data and clarification of methods would improve the strength of these conclusions. These findings, which integrate imaging, genetics, and computational modeling, provide a new way to consider host-pathogen interactions in EPEC infections that may lead to improved therapies.

Reviewed by eLife

A retrograde transit filter mediated by optineurin controls mitostasis in distal axons

1. Natalia A. Marahori
2. Barbara Gailer
3. Martina Schifferer
4. Tatjana Kleele
5. Anna Iatroudi
6. Shabab B. Hannan
7. Petros Avramopoulos
8. Stefan Engelhardt
9. Melike Lakadamyali
10. Monika S. Brill
11. Thomas Misgeld

Reviewed by PREreview

mTORC1 signaling modulate microtubule tyrosination/detyrosination status to regulate lysosome dynamics

1. Jeganath Ammavasai
2. Nitin Mohan

Reviewed by PREreview