Cell Biology

Rho-ROCK liberates sequestered claudin for rapid de novo tight junction formation

1. Yuma Cho
2. Akari Taniguchi
3. Akiharu Kubo
4. Junichi Ikenouchi

• Curated by eLife

  eLife Assessment

  This paper identifies a crucial step in the regulation of tight junction formation by identifying Rho-ROCK activity-dependent activation of the serine protease Matriptase, making Claudins available for tight junction formation. The reviewers were satisfied with the revisions and found the work important and the approach convincing.

Reviewed by eLife

Targeting the WSB2–NOXA axis in cancer cells for enhanced sensitivity to BCL-2 family protein inhibitors

1. Dongyue Jiao
2. Kun Chang
3. Jiamin Jin
4. Yingji Chen
5. Mo Ren
6. Yucong Zhang
7. Kun Gao
8. Yaoting Xu
9. Lixin Wang
10. Chenji Wang

• Curated by eLife

  eLife Assessment

  This study reports a fundamental observation concerning cell death regulation by the anti-apoptotic BCL2 family NOXA. The authors convincingly demonstrate that NOXA is destabilized through the interaction with WSB2, a substrate receptor in CRL5 ubiquitin ligase complex, sensitizing the cells to treatments. These are key findings for cell biologists and cancer researchers as they identified a new target impacting drug responsiveness in cancer therapies.

Reviewed by eLife

Human formin FHOD3-mediated actin elongation is required for sarcomere integrity in cardiomyocytes

1. Dylan A Valencia
2. Angela N Koeberlein
3. Haruko Nakano
4. Akos Rudas
5. Aanand A Patel
6. Airi Harui
7. Cassandra Spencer
8. Atsushi Nakano
9. Margot E Quinlan

• Curated by eLife

  eLife Assessment

  Valencia et al. combine elegant in vitro biochemical experiments with functional assays in cardiomyocytes to determine which properties of the FHOD3 formin are essential for sarcomere assembly. Using separation-of-function mutants, they show that FHOD3's elongation activity, rather than its nucleation, capping, or bundling activities, is key to its sarcomeric function. This is an important finding and the data presented in the manuscript are convincing; however, the presence of FHOD3 at filament barbed ends in the TIRF elongation assays should probably be verified directly in a future study.

Reviewed by eLife

Mitochondrial fission during mitophagy requires both inner and outer mitofissins

1. Kentaro Furukawa
2. Tatsuro Maruyama
3. Shun-ichi Yamashita
4. Keiichi Inoue
5. Tomoyuki Fukuda
6. Nobuo N. Noda
7. Tomotake Kanki

Reviewed by Review Commons

Cardiac neurons expressing a glucagon-like receptor mediate cardiac arrhythmia induced by high-fat diet in Drosophila

1. Yunpo Zhao
2. Jianli Duan
3. Joyce van de Leemput
4. Zhe Han

• Curated by eLife

  eLife Assessment

  This study reports useful information on the mechanisms by which a high-fat diet induces arrhythmias in the model organism Drosophila. Specifically, the authors propose that adipokinetic hormone (Akh) secretion is increased with this diet, and through binding of Akh to its receptor on cardiac neurons, arrhythmia is induced. The authors have revised their manuscript but the evidence remains incomplete. Nonetheless, the data presented will be helpful to those who wish to extend the research to a more complex model system, such as the mouse.

Reviewed by eLife

Optogenetic actin network assembly on lipid bilayer uncovers the network density-dependent functions of actin-binding proteins

1. Kei Yamamoto
2. Makito Miyazaki

Reviewed by Arcadia Science

A Commander-independent function of COMMD3 in endosomal trafficking

1. Galen T Squiers
2. Chun Wan
3. James Gorder
4. Harrison Puscher
5. Jingshi Shen

• Curated by eLife

  eLife Assessment

  This important study explores the mechanisms underlying the maintenance of cell surface protein levels. The authors present solid evidence to support their claims, though the addition of certain validation experiments could have further strengthened the conclusions. This work will be of particular interest to cell biologists focused on membrane trafficking.

Reviewed by eLife

The triad interaction of ULK1, ATG13, and FIP200 is required for ULK complex formation and autophagy

1. Yutaro Hama
2. Yuko Fujioka
3. Hayashi Yamamoto
4. Noboru Mizushima
5. Nobuo N Noda

• Curated by eLife

  eLife Assessment

  Building on previous structural studies, this work provides valuable new insights into the architecture of the autophagy initiation complex, comprising ULK1, ATG13, and FIP200. The authors present their findings with solid supporting evidence, making this study a significant contribution to the autophagy field.

Reviewed by eLife

Reversible One-way Lipid Transfer at ER–Autophagosome Membrane Contact Sites via Bridge-like Lipid Transfer Protein Atg2

1. Li Hao
2. Tomoki Midorikawa
3. Yuta Ogasawara
4. Yutaro Hama
5. Huichao Lang
6. Nobuo N. Noda
7. Kuninori Suzuki

Reviewed by Review Commons