Cell Biology

Cell cycle-regulated transcriptional pausing of Drosophila replication-dependent histone genes

1. James P. Kemp
2. Mark S. Geisler
3. Mia Hoover
4. Chun-Yi Cho
5. Patrick H. O’Farrell
6. William F. Marzluff
7. Robert J. Duronio

Reviewed by Review Commons

Emergence of Dip2-mediated specific DAG-based PKC signalling axis in eukaryotes

1. Sakshi Shambhavi
2. Sudipta Mondal
3. Arnab Chakraborty
4. Nikita Shukla
5. Bapin Kumar Panda
6. Santhosh Kumar
7. Priyadarshan Kinatukara
8. Biswajit Pal
9. Siddhesh S Kamat
10. Rajan Sankaranarayanan

• Curated by eLife

  eLife Assessment

  This is an interesting study that adds useful new data addressing how different DAG pools influence cellular signaling. The study dissects how the enzyme Dip2 modulates the minor lipid signaling DAG pool, which is distinct from the lipid metabolism DAG pool utilized in membrane production. Overall the analysis is solid and broadly supports the claims.

Reviewed by eLife

Evidence of centromeric histone 3 chaperone involved in DNA damage repair pathway

1. Prakhar Agarwal
2. Santanu Kumar Ghosh

Reviewed by Review Commons

A minimal mathematical model for polarity establishment and centralspindlin-independent cytokinesis

1. Ondrej Maxian
2. Katrina M. Longhini
3. Michael Glotzer

Reviewed by Review Commons

Sexually dimorphic ATF4 expression in the fat confers female stress tolerance in Drosophila melanogaster

1. Lydia Grmai
2. Melissa Mychalczuk
3. Aditya Arkalgud
4. Deepika Vasudevan

Reviewed by Review Commons

Disease-associated programming of cell memory in glycogen storage disorder type 1a

1. U Sprecher
2. J D’Souza
3. K Mishra
4. N Muchtar
5. O Shalev
6. A Eliassaf
7. A Morshina
8. A Canella Miliano
9. G Mithieux
10. F Rajas
11. S Avraham
12. F Castellani Moses
13. H Kauffman
14. Y Bergman
15. N Garti
16. S Garti
17. M Linial
18. Y Anikster
19. O Kakhlon
20. M Weil

Reviewed by Review Commons

NFAT2 drives both Orai3 transcription and protein degradation by harnessing the differences in epigenetic landscape

1. Sharon Raju
2. Akshay Sharma
3. Gyan Ranjan
4. Rajender K Motiani

Reviewed by Review Commons

Differential spatial regulation and activation of integrin nanoclusters inside focal adhesions

1. Sarah Keary
2. Nicolas Mateos
3. Felix Campelo
4. Maria F Garcia-Parajo

• Curated by eLife

  eLife Assessment

  In this manuscript, the authors analyse the nanoscale localisation of α5β1 and αVβ3 integrins in integrin adhesion complexes (IAC) by dual-colour STORM and assess the spatial organisation at the nano and mesoscale of their main adaptors (paxillin, talin and vinculin). This is an important work that provides detailed analyses that reveal how elements of these complex structures are really organised at the nanoscale, an essential perspective for a better understanding of how IACs function and regulate mechanotransduction processes. The evidence presented is solid, with super-resolution imaging experiments conducted using a single, validated methodology and subsequent computational modelling that enabled a quantitative assessment of the resulting data.

Reviewed by eLife

Nim1-related kinases regulate septin organization and cytokinesis by modulating Hof1 at the cell division site

1. Bindu Bhojappa
2. Anubhav Dhar
3. Bagyashree VT
4. Jayanti Kumari
5. Freya Cardozo
6. Vaseef Rizvi
7. Saravanan Palani

• Curated by eLife

  eLife Assessment

  This valuable study determines the functional requirements for localization and activity of S. cerevisiae septin-associated kinases using in vivo imaging, in vitro and in vivo protein-protein interaction assays, and an instructive in vivo "tethering" approach. In addition to confirming previous results, the study offers evidence that the septin-associated kinases may directly interact with the contractile ring machinery. Although the experiments appear to have been conducted correctly, the quantitative analysis of some experiments is incomplete and should be improved to strengthen the conclusions.

Reviewed by eLife

Glutamine catabolism supports amino acid biosynthesis and suppresses the integrated stress response to promote photoreceptor survival

1. Moloy T Goswami
2. Eric Weh
3. Shubha Subramanya
4. Katherine M Weh
5. Hima Bindu Durumutla
6. Heather Hager
7. Nicholas Miller
8. Sraboni Chaudhury
9. Anthony Andren
10. Peter Sajjakulnukit
11. Li Zhang
12. Cagri Besirli
13. Costas A Lyssiotis
14. Thomas J Wubben

• Curated by eLife

  eLife Assessment

  Goswami and colleagues used rod-specific Gls1 (the gene encoding glutaminase 1) knockout mice to investigate the role of GLS1 in photoreceptor health when GLS1 was deleted from developing or adult photoreceptor cells. This study is fundamental as it shows the critical role of glutamine catabolism in photoreceptor cell health using in vivo model systems. The evidence supporting the authors' claims is compelling. The studies add new insight into how specific metabolites support vision.

Reviewed by eLife