Cell Biology

The dynamics of ciliogenesis in prepubertal mouse meiosis reveal new clues about testicular maturation during puberty

1. I Pérez-Moreno
2. P López-Jiménez
3. H Zapata
4. S Pérez-Martín
5. M López-Panadés
6. B Barbeito
7. J Urtasun-Elizari
8. I Roig
9. FR Garcia-Gonzalo
10. J Page
11. R Gómez

Reviewed by Review Commons

Non-synaptic exocytosis along the axon shaft and its regulation by the submembrane periodic skeleton

1. Theresa Wiesner
2. Christopher Parperis
3. Fanny Boroni-Rueda
4. Nicolas Jullien
5. Afonso Mendes
6. Léa Marie
7. Louisa Mezache
8. Marie-Jeanne Papandréou
9. Ricardo Henriques
10. Christophe Leterrier

Reviewed by Review Commons

The environmental stress response controls the biophysical properties of the cytoplasm and is critical for survival in quiescence

1. Lorena Kronig
2. Carmen A. Weber
3. Pablo Aurelio Gómez-García
4. Jonas S. Fischer
5. Christian Doerig
6. Agnès Michel
7. Paolo Ronchi
8. Sarah Khawaja
9. Paola Picotti
10. Benoît Kornmann
11. Karsten Weis

Reviewed by Review Commons

Cardiac neurons expressing a glucagon-like receptor mediate cardiac arrhythmia induced by high-fat diet in Drosophila

1. Yunpo Zhao
2. Jianli Duan
3. Joyce van de Leemput
4. Zhe Han

• Curated by eLife

  eLife Assessment

  This study reports useful information on the mechanisms by which a high-fat diet induces arrhythmias in the model organism Drosophila. Specifically, the authors propose that adipokinetic hormone (Akh) secretion is increased with this diet, and through binding of Akh to its receptor on cardiac neurons, arrhythmia is induced. The authors have revised their manuscript, but in some areas the evidence remains incomplete, which the authors say future studies will be directed to closing the present gaps. Nonetheless, the data presented will be helpful to those who wish to extend the research to a more complex model system, such as the mouse.

Reviewed by eLife

Specific GPCRs elicit unique extracellular vesicle miRNA array signatures

1. Xiao Shi
2. Michelle C Palumbo
3. Sheila Benware
4. Jack Wiedrick
5. Sheila Markwardt
6. Aaron J Janowsky

• Curated by eLife

  eLife Assessment

  This study presents valuable findings by demonstrating that specific GPCR subtypes induce distinct extracellular vesicle miRNA signatures, highlighting a potential novel mechanism for intercellular communication with implications for receptor pharmacology within the field. The evidence is solid, however, more experiments are needed to determine whether the distinct extracellular vesicle miRNA signatures result from GPCR-dependent miRNA expression or GPCR-dependent incorporation of miRNAs into extracellular vesicles.

Reviewed by eLife

Accessibility of the unstructured α-tubulin C-terminal tail is controlled by microtubule lattice conformation

1. Takashi Hotta
2. Morgan L Pimm
3. Ezekiel C Thomas
4. Yang Yue
5. Patrick DeLear
6. Lynne Blasius
7. Michael A Cianfrocco
8. Morgan E DeSantis
9. Ryota Horiuchi
10. Takumi Higaki
11. David Sept
12. Ryoma Ohi
13. Kristen J Verhey

• Curated by eLife

  eLife Assessment

  This fundamental work reveals that the accessibility of the unstructured C-terminal tail of α-tubulin differs with the state of the microtubule lattice. Accessibility increases with the expansion of the lattice induced by GTP and certain MAPs, which can then dictate the subsequent interactions between MAPs and microtubules, and post-translational modifications of tubulin tails. The evidence supporting the conclusion is compelling, although the characterisation of the probes does not answer whether they directly affect the lattice or expose the C-terminal tail of α-tubulin. The probes can be used as tools in the future to study differences in microtubule lattice regulation under different conditions both in vitro and in vivo. This work will be of great interest to the cytoskeleton field.

Reviewed by eLife

Plasmin activity and sterile inflammation synergize to promote lethal embryonic liver degeneration

1. Meng-Ling Wu
2. Courtney T. Griffin

Reviewed by Review Commons

Defining the chromatin-associated protein landscapes on Trypanosoma brucei repetitive elements using synthetic TALE proteins

1. Roberta Carloni
2. Tadhg Devlin
3. Pin Tong
4. Christos Spanos
5. Tanya Auchynnikava
6. Juri Rappsilber
7. Keith R Matthews
8. Robin C Allshire

• Curated by eLife

  eLife Assessment

  This work significantly advances our understanding of chromatin organization within regions of repetitive sequences in the parasitic protozoan Trypanosoma brucei. Using cutting edge interdisciplinary tools, the authors provide compelling evidence for two discrete types of repetitive DNA element-associated proteins- one set involved in essential centromere function; and, the other involved in glycoprotein antigenic variation via homologous recombination. Thus, these fundamental findings have implications for this parasite's biology, and for therapeutic targeting in kinetoplastid diseases. This work will be exciting to those in the centromere/mitosis and parasite immunity fields.

  [Editors' note: this paper was reviewed by Review Commons.]

Reviewed by eLife, Review Commons

KANK2 at focal adhesion regulates their maintenance and dynamics, while at fibrillar adhesions it influences cell migration via microtubule-dependent mechanism

1. Nikolina Stojanović
2. Anja Rac
3. Marija Lončarić
4. Ana Tadijan
5. Mladen Paradžik
6. Marta Acman
7. Jonathan D. Humphries
8. Martin J. Humphries
9. Andreja Ambriović-Ristov

Reviewed by preLights

Histone methyltransferase DOT1L differentially affects the development of dendritic cell subsets

1. Rianne G. Bouma
2. Willem-Jan de Leeuw
3. Aru Z. Wang
4. Muddassir Malik
5. Joeke G.C. Stolwijk
6. Veronique A.L. Konijn
7. Anne Mensink
8. Natalie Proost
9. Maarten K. Nijen Twilhaar
10. Tibor van Welsem
11. Negisa Seyed Toutounchi
12. Alsya J. Affandi
13. Jip T. van Dinter
14. Fred van Leeuwen
15. Joke M.M. den Haan

Reviewed by Review Commons