Cell Biology

p66Shc Mediates SUMO2-induced Endothelial Dysfunction

1. Jitendra Kumar
2. Shravan K Uppulapu
3. Sujata Kumari
4. Kanika Sharma
5. William Paradee
6. Ravi Prakash Yadav
7. Vikas Kumar
8. Santosh Kumar

• Curated by eLife

  eLife Assessment

  This study offers valuable insights into the role of post-translational modifiers, specifically SUMO2ylation at K81 in p66Shc, and its impact on endothelial function through reactive oxygen species. A series of compelling experiments demonstrated that lysine 81 of p66Shc is the site of SUMO2 conjugation, which is crucial for mitochondrial localization and essential for S36 phosphorylation, leading to specific pathological effects. The combination of cell overexpression and animal studies provides solid data supporting this mechanistic link.

Reviewed by eLife

Ubiquitination-activated TAB–TAK1–IKK–NF-κB axis modulates gene expression for cell survival in the lysosomal damage response

1. Akinori Endo
2. Chikage Takahashi
3. Yasumasa Nishito
4. Keiji Tanaka
5. Yukiko Yoshida

• Curated by eLife

  eLife Assessment

  This study presents the important finding that lysosomal damage triggers inflammatory signaling through ubiquitination and the TAB-TAK1-IKK-NF-kB axis. The data obtained from the unbiased transcriptomic and proteomic analyses are convincing and provide invaluable information to the field. Although further experiments will be required to clarify how TAB2/3 are activated, this work will be of interest to researchers in the fields of organelle biology and inflammation.

Reviewed by eLife

Mutations that prevent phosphorylation of the BMP4 prodomain impair proteolytic maturation of homodimers leading to lethality in mice

1. Hyung-Seok Kim
2. Mary L Sanchez
3. Joshua Silva
4. Heidi L Schubert
5. Rebecca Dennis
6. Christopher P Hill
7. Jan L Christian

• Curated by eLife

  eLife Assessment

  This fundamental work presents two clinically relevant BMP4 mutations that contribute to vertebrate development. The compelling evidence, both from wet lab and AI generated predictions, supports that the site-specific cleavage at the BMP4 pro-domain precisely regulates its function and provides mechanistic insight how homodimers and heterodimers behave differently. The work will be of broad interest to researchers working on growth factor signaling mechanisms and vertebrate development.

Reviewed by eLife

Monocyte-derived macrophage recruitment mediated by TRPV1 is required for eardrum wound healing

1. Yunpei Zhang
2. Pingting Wang
3. Lingling Neng
4. Kushal Sharma
5. Allan Kachelmeier
6. Xiaorui Shi

• Curated by eLife

  eLife Assessment

  This study exploring the role of TRPV1 signaling in recruiting macrophages and promoting angiogenesis during tympanic membrane wound healing presents useful findings. However, the strength of evidence supporting the central claims is incomplete, as the mechanistic links between TRPV1 activation and immune cell recruitment remain largely correlative and rely heavily on previously published datasets without sufficient functional validation. The work will be of interest to researchers studying wound healing and sensory-immune interactions, though substantial revisions are needed to support its broader significance.

Reviewed by eLife

Dual regulation of the unfolded protein response by IGF2BP3 during ER stress

1. Aleksandra S Anisimova
2. Harald Hornegger
3. Irmgard Fischer
4. Gijs A Versteeg
5. Stefan L Ameres
6. G Elif Karagöz

Reviewed by PREreview

Random UV Mutagenesis for the production of Chlorella vulgaris mutants with low chlorophyll content for the food industry

1. Ivan N. Ivanov
2. Jiří Kopecký
3. Karolína Štěrbová
4. Pavel Hrouzek
5. Martin Lukeš
6. Kateřina Bišová
7. Setyo Budi Kurniawan

Reviewed by Arcadia Science

Brain-derived exosomal hemoglobin transfer contributes to neuronal mitochondrial homeostasis under hypoxia

1. Zhengming Tian
2. Yuning Li
3. Feiyang Jin
4. Zirui Xu
5. Yakun Gu
6. Mengyuan Guo
7. Qianqian Shao
8. Yingxia Liu
9. Hanjiang Luo
10. Yue Wang
11. Suyu Zhang
12. Chenlu Yang
13. Xin Liu
14. Xunming Ji
15. Jia Liu

• Curated by eLife

  eLife Assessment

  This valuable paper analyses the role of endogenous CNS hemoglobin in protecting mitochondrial homeostasis in hypoxic conditions. The work is solid and opens the doors to future work in this field. However, it leaves many questions open regarding CNS-specific ischemia/hypoxia that should be considered in future work. In particular, a whole-body hypoxia model may liberate exosomes from other hypoxic organs, which may contribute to the protective effect. Overall, this work has the potential to be of broad interest to the neuroscience and hypoxia communities.

Reviewed by eLife

Polarity reversal of stable microtubules during neuronal development

1. Malina K. Iwanski
2. Albert K. Serweta
3. H. Noor Verwei
4. Bronte C. Donders
5. Lukas C. Kapitein

Reviewed by Review Commons

Drosophila larval gut transcriptome reveals a microbe-mediated intestinal tissue growth via Ecdysone during adaptive growth

1. Longwei Bai
2. Stéphanie Bellemin
3. Coralie Drelon
4. Pauline Joncour
5. Elodie Guillemot
6. Maura Strigini
7. Benjamin Gillet
8. François Leulier
9. Cathy Isaura Ramos

Reviewed by Review Commons

AARS2 ameliorates myocardial ischemia via fine-tuning PKM2-mediated metabolism

1. Zongwang Zhang
2. Lixia Zheng
3. Yang Chen
4. Yuanyuan Chen
5. Junjie Hou
6. Chenglu Xiao
7. Xiaojun Zhu
8. Shi-Min Zhao
9. Jing-Wei Xiong

• Curated by eLife

  eLife Assessment

  This important study highlights the essential role of AARS2 in safeguarding cardiomyocytes against ischemic stress by modulating energy metabolism towards glycolysis via PKM2. This mechanism unveils a promising new therapeutic target for treating myocardial infarction. Convincing findings are underpinned by a comprehensive dataset, including cardiomyocyte-specific genetic modifications, functional assays, and ribosome profiling, all collectively providing strong evidence for the critical involvement of the AARS2-PKM2 signalling pathway in cardiac protection.

Reviewed by eLife