Cell Biology

The bile acid receptor TGR5 regulates the hematopoietic support capacity of the bone marrow niche

1. Alejandro Alonso-Calleja
2. Alessia Perino
3. Frédérica Schyrr
4. Silvia Ferreira Lopes
5. Vasiliki Delitsikou
6. Antoine Jalil
7. Ulrike Kettenberger
8. Dominique P. Pioletti
9. Kristina Schoonjans
10. Olaia Naveiras

• Curated by eLife

  eLife assessment

  This study investigates the role of the bile acid receptor TGR5 in adult hematopoiesis of the mouse model. The findings are potentially useful because the loss of TGR5 leads to dysregulation of bone marrow adipose tissue (BMAT) that has emerging regulatory functions. However, the study is still incomplete because the mechanism of TGR5 is not clear, the stromal cells expressing TGR5 have not been well defined, and there is not strong evidence for the role of TGR5 in recovery from transplant stress.

Reviewed by eLife

Excessive F-Actin and Microtubule Formation Mediates Primary Cilia Shortening and Loss in Response to Increased Extracellular Osmotic Pressure

1. Hiroshi Otani
2. Ryota Nakazato
3. Faryal Ijaz
4. Kanae Koike
5. Koji Ikegami

Reviewed by Arcadia Science

Autoacetylation-mediated phase separation of TIP60 is critical for its functions

1. Shraddha Dubey
2. Himanshu Gupta
3. Ashish Gupta

• Curated by eLife

  eLife assessment

  This is a valuable study on K187 acetylation of the nuclear protein, TIP60, required for its phase separation and function. The evidence supporting the primary conclusion is incomplete and warrants more scrutiny.

Reviewed by eLife

CEP44 is required for maintaining centriole duplication and spindle integrity

1. Donghui Zhang
2. Wenlu Wei
3. Xiaopeng Zou
4. Hui Meng
5. Fangyuan Li
6. Minjun Yao
7. Junling Teng
8. Ning Huang
9. Jianguo Chen

• Curated by eLife

  eLife assessment

  This useful study investigates the role of the centrosomal protein CEP44 in centriole duplication and mitotic spindle formation. While the analysis of CEP44 mitotic phosphorylation and spindle recruitment is solid, the characterization of CEP44's role at centrioles is incomplete and would benefit from additional controls and analyses. Since the work links CEP44 reduced expression to poor survival in breast cancer patients, it is of interest not only to cell biologists but also to cancer researchers.

Reviewed by eLife

Ex vivo expansion potential of murine hematopoietic stem cells is a rare property only partially predicted by phenotype

1. Qinyu Zhang
2. Rasmus Olofzon
3. Anna Konturek-Ciesla
4. Ouyang Yuan
5. David Bryder

• Curated by eLife

  eLife assessment

  This study presents a valuable dissection on how functional HSCs are expanded in PVA cultures. The functional and multi-omic analyses provided are convincing, although the additional data and their analysis provided during revision could have been included in the test to assist readers and to strengthen the published manuscript. Nevertheless, the present work will be of value for stem cell biologists interested in HSC regulation.

Reviewed by eLife

Mantis: High-throughput 4D imaging and analysis of the molecular and physical architecture of cells

1. Ivan E Ivanov
2. Eduardo Hirata-Miyasaki
3. Talon Chandler
4. Rasmi Cheloor-Kovilakam
5. Ziwen Liu
6. Soorya Pradeep
7. Chad Liu
8. Madhura Bhave
9. Sudip Khadka
10. Carolina Arias
11. Manuel D Leonetti
12. Bo Huang
13. Shalin B Mehta

Reviewed by Arcadia Science

Yeast TLDc domain-containing proteins control assembly and subcellular localization of the V-ATPase

1. Samira Klössel
2. Ying Zhu
3. Lucia Amado
4. Daniel D. Bisinski
5. Julia Ruta
6. Fan Liu
7. Ayelén González Montoro

Reviewed by Review Commons

A TOPBP1 allele causing male infertility uncouples XY silencing dynamics from sex body formation

1. Carolline Ascenção
2. Jennie R Sims
3. Alexis Dziubek
4. William Comstock
5. Elizabeth A Fogarty
6. Jumana Badar
7. Raimundo Freire
8. Andrew Grimson
9. Robert S Weiss
10. Paula E Cohen
11. Marcus B Smolka

• Curated by eLife

  eLife assessment

  This important study reports a new mutant mouse line with compromised function of a DNA damage response protein. The evidence supporting the conclusion that the mutants display defective maintenance of meiotic sex chromosome inactivation is solid. This work is of interest to biomedical researchers working on meiosis and meiotic sex chromosome inactivation.

Reviewed by eLife

Organelle landscape analysis using a multi-parametric particle-based method

1. Yoshitaka Kurikawa
2. Ikuko Koyama-Honda
3. Keiko Igarashi
4. Norito Tamura
5. Seiichi Koike
6. Noboru Mizushima

Reviewed by Review Commons

Calcium transients trigger switch-like discharge of prostaglandin E2 in an extracellular signal-regulated kinase-dependent manner

1. Tetsuya Watabe
2. Shinya Yamahira
3. Kanako Takakura
4. Dean Thumkeo
5. Shuh Narumiya
6. Michiyuki Matsuda
7. Kenta Terai

• Curated by eLife

  eLife assessment

  This important study reports on the dynamics of PKA investigated at the single-cell level in vitro and in epithelia in vivo. Using different fluorescent biosensors and optogenetic actuators, the authors dissect the signaling pathway responsible for PKA waves, finding that PKA activation is a consequence of PGE2 release, which in turn is triggered by calcium pulses, requiring high ERK activity. The evidence supporting the claims is solid. At this stage the work is still partly descriptive in nature, and additional measurements would increase the strength of mechanistic insights and physiological relevance.

Reviewed by eLife