Cell Biology

FAM134B regulates ER-to-lysosome-associated degradation of misfolded proteins upon pharmacologic or genetic inactivation of ER-associated degradation

1. Elisa Fasana
2. Ilaria Fregno
3. Carmela Galli
4. Maurizio Molinari

Reviewed by Review Commons

Mapping variation in the morphological landscape of human cells with optical pooled CRISPRi screening

1. Ramon Lorenzo D. Labitigan
2. Adrian L. Sanborn
3. Cynthia V. Hao
4. Caleb K. Chan
5. Nathan M. Belliveau
6. Eva M. Brown
7. Mansi Mehrotra
8. Julie A. Theriot

• Curated by eLife

  eLife assessment

  In this important study by Theriot et al., the authors utilize an impressive set of innovative approaches to conduct a CRISPRi pooled screen in human cells using large-scale microscopy screen data. They leverage an improved barcoding approach to identify genes targeted in specific cells and examine the effects on cell morphology using high-dimensional phenotypic analysis. The method and data presented are compelling.

Reviewed by eLife

Neural network emulation of the human ventricular cardiomyocyte action potential for more efficient computations in pharmacological studies

1. Thomas Grandits
2. Christoph M Augustin
3. Gundolf Haase
4. Norbert Jost
5. Gary R Mirams
6. Steven A Niederer
7. Gernot Plank
8. András Varró
9. László Virág
10. Alexander Jung

• Curated by eLife

  eLife assessment

  This valuable prospective study develops a new tool to accelerate pharmacological studies by using neural networks to emulate the human ventricular cardiomyocyte action potential. The evidence supporting the conclusions is convincing, based on using a large and high-quality dataset to train the neural network emulator. There are nevertheless a few areas in which the article may be improved through validating the neural network emulators against extensive experimental data. In addition, the article may be improved through delineating the exact speed-up achieved and the scope for acceleration. The work will be of broad interest to scientists working in cardiac simulation and quantitative system pharmacology.

Reviewed by eLife

Morphogen-driven human iPSCs differentiation in 3D in vitro models of gastrulation is precluded by physical confinement

1. Haneen S. Alsehli
2. Errin Roy
3. Thomas Williams
4. Alicja Kuziola
5. Yunzhe Guo
6. Jeremy Green
7. Eileen Gentleman
8. Davide Danovi

Reviewed by Review Commons

A novel imaging method (FIM-ID) reveals that myofibrillogenesis plays a major role in the mechanically induced growth of skeletal muscle

1. Kent W Jorgenson
2. Jamie E Hibbert
3. Ramy KA Sayed
4. Anthony N Lange
5. Joshua S Godwin
6. Paulo HC Mesquita
7. Bradley A Ruple
8. Mason C McIntosh
9. Andreas N Kavazis
10. Michael D Roberts
11. Troy A Hornberger

• Curated by eLife

  eLife assessment

  The work by Hornberger and team presents a novel workflow for the visualisation of myofibrils with high resolution and contrast that will be highly valued by the scientific community. The methods include solid validation of both sample preparation and analysis, and have been used to make the fundamental discovery of myofibrillogenesis as the mechanism of mechanical loading-induced growth. Whether this mechanism is present in other settings of muscle growth (i.e., non-loading), other striated tissue (e.g myocardium), or is sex-dependent, will require future experiments.

Reviewed by eLife

Bridge-like lipid transfer protein family member 2 suppresses ciliogenesis

1. Jan Parolek
2. Christopher G. Burd

Reviewed by Review Commons

An open-source, high-resolution, automated fluorescence microscope

1. Ando Christian Zehrer
2. Ana Martin-Villalba
3. Benedict Diederich
4. Helge Ewers

• Curated by eLife

  eLife assessment

  This important study provides compelling evidence that the low-cost and open-hardware UC2 microscopy framework can be expanded to enable single-molecule localization microscopy. The authors managed to fit the instrumentation and control thereof in a unit that can be placed in a small stage-top-incubator. Together with providing adapted software for data acquisition and data analysis, the UC.STORM setup can rival the capabilities of comparable commercial instruments at a fraction of the costs.

Reviewed by eLife

Liver regeneration by a population of midzone-located mesenchymal-hepatocyte hybrid cells

1. Guo Yu
2. Shaoyang Zhang
3. Ana Romo
4. Soma Biswas
5. Baojie Li
6. Jing Li

• Curated by eLife

  eLife assessment

  This study is partly useful as it corroborates what is already known about the elevated proliferation capacity of mid lobular hepatocytes in liver regeneration. Lineage tracing and scRNAseq studies are powerful for the investigation of such heterogeneous hepatocyte proliferation capacity. Nevertheless, based on experimental limitations, incomplete method description and inadequate data analyses the presented data are insufficient to support the proposed conclusions of a mesenchymal-hepatocyte hybrid population in the murine liver.

Reviewed by eLife

CDK-4 regulates nucleolar size and metabolism at the cost of late-life fitness in C. elegans

1. Rachel Webster
2. Maria Quintana
3. Bin Yu
4. Stacey Fluke
5. Ran Kafri
6. W. Brent Derry

Reviewed by Arcadia Science

The bile acid receptor TGR5 regulates the hematopoietic support capacity of the bone marrow niche

1. Alejandro Alonso-Calleja
2. Alessia Perino
3. Frédérica Schyrr
4. Silvia Ferreira Lopes
5. Vasiliki Delitsikou
6. Antoine Jalil
7. Ulrike Kettenberger
8. Dominique P. Pioletti
9. Kristina Schoonjans
10. Olaia Naveiras

• Curated by eLife

  eLife assessment

  This study investigates the role of the bile acid receptor TGR5 in adult hematopoiesis of the mouse model. The findings are potentially useful because the loss of TGR5 leads to dysregulation of bone marrow adipose tissue (BMAT) that has emerging regulatory functions. However, the study is still incomplete because the mechanism of TGR5 is not clear, the stromal cells expressing TGR5 have not been well defined, and there is not strong evidence for the role of TGR5 in recovery from transplant stress.

Reviewed by eLife