Cell Biology

Mechanical coupling coordinates microtubule growth

1. Bonnibelle K Leeds
2. Katelyn F Kostello
3. Yuna Y Liu
4. Christian R Nelson
5. Sue Biggins
6. Charles L Asbury

• Curated by eLife

  eLife assessment

  In this technically advanced and important piece of work, the authors study the coordination of microtubule growth in kinetochore fibers using force spectroscopy and numerical simulations. With compelling evidence the authors address the question of how microtubules, which naturally exhibit variable growth rates, can coordinate their behavior by mechanical coupling so as to function as a single unit in generating forces during chromosome segregation.

Reviewed by eLife

Metabolic regulation of misfolded protein import into mitochondria

1. Yuhao Wang
2. Linhao Ruan
3. Jin Zhu
4. Xi Zhang
5. Alexander Chih-Chieh Chang
6. Alexis Tomaszewski
7. Rong Li

• Curated by eLife

  eLife assessment

  This study makes a connection between cellular metabolism and proteostasis through MAGIC, a previously proposed protein quality control pathway of clearance of cytosolic misfolded and aggregated proteins by importing into mitochondria. The authors reveal the role of Snf1, a yeast AMPK, in preventing the import of misfolded proteins to mitochondria for MAGIC controlled by the transcription factor Hap4, depending on the cellular metabolic status. The key message is important, although the evidence for physiological relevance of MAGIC for overall cellular proteostasis and its molecular regulation by Snf1 remains incomplete.

Reviewed by eLife, Life Science Editors Foundation

The loss of DNA polymerase epsilon accessory subunits POLE3-POLE4 leads to BRCA1-independent PARP inhibitor sensitivity

1. Hasan Mamar
2. Roberta Fajka-Boja
3. Mónika Mórocz
4. Eva Pinto Jurado
5. Siham Zentout
6. Alexandra Mihuț
7. Anna Georgina Kopasz
8. Mihály Mérey
9. Rebecca Smith
10. Lajos Haracska
11. Sébastien Huet
12. Gyula Timinszky

Reviewed by preLights

Tight nanoscale clustering of Fcγ receptors using DNA origami promotes phagocytosis

1. Nadja Kern
2. Rui Dong
3. Shawn M Douglas
4. Ronald D Vale
5. Meghan A Morrissey

Reviewed by Review Commons, preLights

Mild mitochondrial impairment enhances innate immunity and longevity through ATFS‐1 and p38 signaling

1. Juliane C Campos
2. Ziyun Wu
3. Paige D Rudich
4. Sonja K Soo
5. Meeta Mistry
6. Julio CB Ferreira
7. T Keith Blackwell
8. Jeremy M Van Raamsdonk

Reviewed by Review Commons

Genetic screen identified PRMT5 as a neuroprotection target against cerebral ischemia

1. Haoyang Wu
2. Peiyuan Lv
3. Jinyu Wang
4. Brian Bennett
5. Jiajia Wang
6. Pishun Li
7. Yi Peng
8. Guang Hu
9. Jiaji Lin

• Curated by eLife

  eLife assessment

  The authors performed a useful RNAi screen to identify epigenetic regulators involved in oxygen-glucose deprivation (OGD)-induced neuronal injury. PRMT5 was identified as a negative regulator of neuronal cell survival after OGD. Solid in vitro and in vivo data suggest that PRMT5 could be a novel therapeutic target for the treatment of ischemic stroke.

Reviewed by eLife

Apoptosis recognition receptors regulate skin tissue repair in mice

1. Olivia Justynski
2. Kate Bridges
3. Will Krause
4. Maria Fernanda Forni
5. Quan M Phan
6. Teresa Sandoval-Schaefer
7. Kristyn Carter
8. Diane E King
9. Henry C Hsia
10. Michael I Gazes
11. Steven D Vyce
12. Ryan R Driskell
13. Kathryn Miller-Jensen
14. Valerie Horsley

• Curated by eLife

  eLife assessment

  The authors studied the mechanisms by which dead cells are removed from the wounded skin in a process called efferocytosis. By analyzing different cell populations in the skin, the authors find that proteins involved in mediating the cell death and marking the cells as undergoing this process are elevated during distinct times in the wound healing program. Interestingly, these same proteins are elevated even higher in diabetic wounds. Finally the authors demonstrate that blocking the process of efferocytosis alters the wound healing program, thus illustrating its importance in effective wound repair.

Reviewed by eLife

Phosphate starvation signaling increases mitochondrial membrane potential through respiration-independent mechanisms

1. Yeyun Ouyang
2. Mi-Young Jeong
3. Corey N Cunningham
4. Jordan A Berg
5. Ashish G Toshniwal
6. Casey E Hughes
7. Kristina Seiler
8. Jonathan G Van Vranken
9. Ahmad A Cluntun
10. Geanette Lam
11. Jacob M Winter
12. Emel Akdogan
13. Katja K Dove
14. Sara M Nowinski
15. Matthew West
16. Greg Odorizzi
17. Steven P Gygi
18. Cory D Dunn
19. Dennis R Winge
20. Jared Rutter

• Curated by eLife

  eLife assessment

  This study presents important findings on the regulation of the phosphate export cycle and identify the phosphatase Sit4 as a crucial player in regulation of the inner membrane potential of mitochondria. Whereas some of the data are convincing, the analyses will profit from deeper insights concerning metabolism alterations (carbon sources, amino acids). The major strength however is a new insight on how the cells use alternative ways for maintaining a critical mitochondrial inner membrane potential, and therefore this study is interesting to the broad audience with interests spanning from bioenergetics, metabolism and organellar and cell biology.

Reviewed by eLife

A transcriptionally active lipid vesicle encloses the injected Chimalliviridae genome in early infection

1. Emily G. Armbruster
2. Phoolwanti Rani
3. Jina Lee
4. Niklas Klusch
5. Joshua Hutchings
6. Lizbeth Y. Hoffman
7. Hannah Buschkaemper
8. Eray Enustun
9. Benjamin A. Adler
10. Koe Inlow
11. Arica R. VanderWal
12. Madelynn Y. Hoffman
13. Daksh Daksh
14. Ann Aindow
15. Amar Deep
16. Zaida K. Rodriguez
17. Chase J. Morgan
18. Majid Ghassemian
19. Thomas G. Laughlin
20. Emeric Charles
21. Brady F. Cress
22. David F. Savage
23. Jennifer A. Doudna
24. Kit Pogliano
25. Kevin D. Corbett
26. Elizabeth Villa
27. Joe Pogliano

Reviewed by Rapid Reviews Infectious Diseases, Arcadia Science

N-WASP-dependent branched actin polymerization attenuates B-cell receptor signaling by increasing the molecular density of receptor clusters

1. Anshuman Bhanja
2. Margaret K Seeley-Fallen
3. Michelle Lazzaro
4. Arpita Upadhyaya
5. Wenxia Song

• Curated by eLife

  eLife assessment

  This is an important study highlighting a distinct role of WASP dependent actin foci in B cell antigen receptor signalling. The evidence supporting the conclusions is compelling. The proposal of higher molecular density in B cell receptor clustering leading to kinase exclusion and attenuated signalling is provocative as it contrasts with models for other antigen receptors.

Reviewed by eLife