Cell Biology

Unconventional components complement the cryptic kinetochore of the ciliate Tetrahymena thermophila

1. Emine I. Ali
2. Maximilian W. D. Raas
3. Laura E. van Rooijen
4. Paula Sobrevals Alcaraz
5. Harmjan R. Vos
6. Eelco C. Tromer
7. Berend Snel
8. Geert J.P.L. Kops

Reviewed by Review Commons

Proteome dynamics reveal Leiomodin 1 as a key regulator of myogenic differentiation

1. Ellen Späth
2. Svenja C Schüler
3. Ivonne Heinze
4. Therese Dau
5. Alberto Minetti
6. Maleen Hofmann
7. Katja Hönzke
8. Julia von Maltzahn
9. Alessandro Ori

• Curated by eLife

  eLife Assessment

  This study provides valuable insights into the regulation of myogenic differentiation by identifying Leiomodin 1 as a modulator of proteome dynamics during myogenic differentiation. The combination of quantitative proteomics with functional perturbation experiments offers solid evidence supporting the idea that SIRT1 influences perturbations of myogenic differentiation upon LMOD1 inactivation. These findings advance our understanding of muscle differentiation and will be of interest to researchers studying muscle development and related pathologies

Reviewed by eLife

The insulin / IGF axis is critically important controlling gene transcription in the podocyte

1. Jennifer A Hurcombe
2. Lusyan Dayalan
3. Fern Barrington
4. Frédéric Burdet
5. Lan Ni
6. Joseph T Coward
7. Mark Ibberson
8. Paul T Brinkkoetter
9. Martin Holzenberger
10. Aaron Jeffries
11. Sebastian Oltean
12. Gavin I Welsh
13. Richard JM Coward

• Curated by eLife

  eLife Assessment

  This valuable study used genetic and pharmacological manipulations of insulin/IGF signaling in renal glomerular podocytes to address the role of insulin/IGF axis in podocytes. Solid data are presented to demonstrate that co-inhibition of insulin/IGF signaling in podocytes led to aberrant splicing of mRNAs, which could contribute to the loss of podocytes in vitro and in vivo in mice. As it stands, the study lacks the assessment of developmental phenotype of podocytes in the mouse model.

  [Editors' note: this paper was reviewed by Review Commons.]

Reviewed by eLife

Uev1A counteracts oncogenic Ras stimuli in both polyploid and diploid cells

1. Qi Zhang
2. Yunfeng Wang
3. Xueli Fu
4. Ziguang Wang
5. Yang Zhang
6. Lizhong Yan
7. Yuejia Wang
8. Muhan Yang
9. Dongze Song
10. Ruixing Zhang
11. Hongru Zhang
12. Shian Wu
13. Shaowei Zhao

• Curated by eLife

  eLife Assessment

  This valuable study examines the role of E2 ubiquitin enzyme, Uev1a in tissue resistance to oncogenic RasV12 in Drosophila melanogaster polyploid germline cells and human cancer cell lines. The solid evidence suggests that Uev1a works with the E3 ligase APC/C to degrade Cyclin A. This work would be of interest to researchers in germline biology and cancer.

Reviewed by eLife

Abnormal shear stress induces ferroptosis in endothelial cells via KLF6 downregulation

1. Jingang Cui
2. Zhiyu Fan
3. Suoqi Ding
4. Jiazhen Zhang
5. Huihong Shen
6. Syeda Armana Zaidi
7. Yongsheng Ding

• Curated by eLife

  eLife Assessment

  This study presents valuable findings on the role of KLF6 in in vitro endothelial cells exposed to altered (high or low) shear stress with a customized microfluidic device to investigate mechanisms of atherosclerosis. The finding that altered shear stress results in endothelial cell ferroptosis through reduced expression of KLF6 is compelling and adds a new layer of complexity to the pathogenesis of atherosclerotic plaques. However, more detailed characterization of ferroptosis is needed.

Reviewed by eLife

LRRK2 mutations block NCOA4 trafficking upon iron overload leading to ferroptotic death

1. Andres Goldman
2. Mai Nguyen
3. Joel Lanoix
4. Chongyang Li
5. Ahmed Fahmy
6. Yong Zhong Xu
7. Erwin Schurr
8. Pierre Thibault
9. Michel Desjardins
10. Heidi McBride

Reviewed by Review Commons

Human receptive endometrial assembloid for deciphering the implantation window

1. Yu Zhang
2. Rusong Zhao
3. Chaoyan Yang
4. Jinzhu Song
5. Peishu Liu
6. Yan Li
7. Boyang Liu
8. Tao Li
9. Changjian Yin
10. Minghui Lu
11. Zhenzhen Hou
12. Chuanxin Zhang
13. Zi-Jiang Chen
14. Keliang Wu
15. Han Zhao

• Curated by eLife

  eLife Assessment

  This important study reports an endometrial organoid culture system mimicking the window of implantation. The evidence supporting the conclusion drawn is convincing. The data will be of interest to embryologists and investigators working on reproductive biology and medicine.

Reviewed by eLife

A bipartite, mutation-tolerant NLS regulates interaction of ΔNp63α with importin alpha, nuclear transport and transcriptional activity

1. Anna Demarinis
2. Sara Esmaeili
3. Crystall M. D. Swarbrick
4. Simone Ranno
5. Silvia Pavan
6. Jade K. Forwood
7. Brian P. McSharry
8. Martin Pal
9. Enzo Di Iorio
10. Gualtiero Alvisi

Reviewed by Review Commons

Prion propagation is controlled by a hierarchical network involving the nuclear Tfap2c and hnRNP K factors and the cytosolic mTORC1 complex

1. Stefano Sellitto
2. Davide Caredio
3. Matteo Bimbati
4. Giovanni Mariutti
5. Martina Cerisoli
6. Lukas Frick
7. Vangelis Bouris
8. Carlos Omar Oueslati Morales
9. Dalila Laura Vena
10. Sandesh Neupane
11. Federico Baroni
12. Kathi Ging
13. Jiang-An Yin
14. Elena De Cecco
15. Andrea Armani
16. Adriano Aguzzi

Reviewed by Review Commons

Unremodeled plasma membrane GPI-anchored proteins trigger aberrant clathrin-mediated endocytosis

1. Li Chen
2. David K. Banfield

Reviewed by PREreview