Cell Biology

MR1 dependent MAIT cell activation is regulated by autophagy associated proteins

1. Prabhjeet Phalora
2. James Ussher
3. Svenja Hester
4. Carl-Philipp Hackstein
5. Emanuele Marchi
6. Jeffrey Y. W. Mak
7. David P. Fairlie
8. Paul Klenerman

Reviewed by Review Commons

The cell cycle variant in multiciliated cells incorporates 2 centriole biogenesis cycles

1. Amélie-Rose Boudjema
2. Rémi Balagué
3. Cayla E Jewett
4. Jacques Serizay
5. Gina M LoMastro
6. Olivier Mercey
7. Adel Al Jord
8. Adrien Candat
9. Marion Faucourt
10. Alexandre Schaeffer
11. Camille Noûs
12. Nathalie Delgehyr
13. Andrew J Holland
14. Nathalie Spassky
15. Alice Meunier

• Curated by eLife

  eLife Assessment

  In this important study, Boudjema et al. use cell culture models and high quality advanced microscopic imaging to provide detailed analyses of the cellular processes underlying centriole amplification, apical migration, and assembly of hundreds of motile cilia in multi-ciliated cells. The authors present convincing evidence showing that in these cells all the molecular and cellular steps controlling centriole biogenesis that in cycling cells extend over almost two cell cycles, occur within a single cell cycle variant. This work provides a better understanding of the regulation and order of these processes and is of interest to all cell biologists and in particular researchers studying centrioles and cilia.

Reviewed by eLife

Cell cycle dependent variation in endocytosis drives phenotypic diversity in M. tuberculosis

1. Neeraja Subhash
2. Sandhya Krishnan Radhakrishnan
3. Hitakshi Vijay
4. Neilay Bhalerao
5. Sahanawaz Molla
6. Anton Iyer
7. Shaon Chakrabarti
8. Varadharajan Sundaramurthy

• Curated by eLife

  eLife Assessment

  This useful study explores how macrophage cell-cycle state may influence endocytosis, Mycobacterium tuberculosis uptake, and the intracellular stress experienced by bacteria. While the question is interesting and the experimental approach has promise, the evidence for the central claim that endocytic capacity is specifically regulated by cell-cycle stage is incomplete. The main concern is that fluorescence-based sorting and total-fluorescence measurements likely covary with cell size, so the reported phenotypes could reflect biomass accumulation or other cell-cycle-associated changes rather than endocytic capacity as the causal determinant. As a result, whilst the study raises a hypothesis that is of importance, additional controls are required before the proposed mechanism can be considered well supported.

Reviewed by eLife

Cytosolic Carboxypeptidase 5 maintains mammalian ependymal multicilia to ensure proper homeostasis and functions of the brain

1. Rubina Dad
2. Yujuan Wang
3. Chuyu Fang
4. Yuncan Chen
5. Yi Zheng
6. Yuan Zhang
7. Xinwen Pan
8. Xinyue Zhang
9. Emily Swanekamp
10. Krish Patel
11. Matthias TF Wolf
12. Zhiguang Yuchi
13. Xueliang Zhu
14. Hui-Yuan Wu

• Curated by eLife

  eLife Assessment

  The authors developed a new Agbl5 KO allele, extending the deletion to the N-terminus of CCP5 to explore its function in mouse ependymal cells and trachea. They show that the KO mice exhibit severe hydrocephalus due to mislocated basal bodies and impaired ciliary beating. The findings are valuable with implications in the subfield of cell biology. The evidence is solid in that the methods, data and analyses largely support the claims with only a few remaining weaknesses.

Reviewed by eLife

Apical actin filament turnover mediated by cyclase-associated protein is required for organization of non-centrosomal microtubules in epithelium

1. Arathi Preeth Babu
2. Sachin Muralidharan
3. Konstantin Kogan
4. Tommi Kotila
5. Ville Hietakangas
6. Jaakko Mattila
7. Minna Poukkula

Reviewed by Review Commons

Detecting Nuclear Pore Complex assembly in living cells

1. Annemiek C Veldsink
2. Jonas S Fischer
3. Hanna M Terpstra
4. Philip J Mannino
5. Eline MF de Lange
6. Sophie Hell
7. Koen J van Benthem
8. Leila J Saba
9. Anton Steen
10. Rifka Vlijm
11. Matthias Heinemann
12. Karsten Weis
13. C Patrick Lusk
14. Liesbeth M Veenhoff

Reviewed by Review Commons