Cell Biology

Single-cell lineage tracing identifies hemogenic endothelial cells in the adult mouse bone marrow

1. Jing-Xin Feng
2. Mei-Ting Yang
3. Lili Li
4. Caiyi C Li
5. Ferenc Livak
6. Jack Chen
7. Yongmei Zhao
8. Dunrui Wang
9. Avinash Bhandoola
10. Naomi Taylor
11. Giovanna Tosato

• Curated by eLife

  eLife Assessment

  This manuscript by Feng et al. provides valuable evidence regarding the hematopoietic differentiation of bone marrow endothelial cells in the adult mouse. Overall, the authors have addressed our main concerns. Solid data now more strongly support long-term multi-lineage reconstitution of the adult hemogenic endothelial cells. However, critical data, especially regarding the endothelial cells' hematopoietic identity and functional capacity, remain insufficient, which limits the strength of the hemogenic claim, especially the assertion that these adult hemogenic ECs generate bona fide HSCs. Additional experiments would be necessary to fully rule out alternative explanations.

Reviewed by eLife

Cell size modulates ferroptosis susceptibility

1. Evgeny Zatulovskiy
2. Magdalena B. Murray
3. Shuyuan Zhang
4. Scott J. Dixon
5. Jan M. Skotheim

Reviewed by Review Commons

Epigenetics and chromatin structure regulate var2csa expression and the placental-binding phenotype in Plasmodium falciparum

1. Todd Lenz
2. Madle Sirel
3. Hannes Hoppe
4. Sulman Shafeeq
5. Karine G Le Roch
6. Ulf Ribacke

• Curated by eLife

  eLife Assessment

  This interesting study presents a multi-OMICs approach to unify different lines of evidence regarding the epigenetic regulation of the key virulence factor causing placental malaria during P. falciparum infection. Most results are confirmatory of previous observations; nonetheless, the claims are supported by convincing evidence. The combinatorial approach chosen here is unprecedented and therefore provides valuable new data. In addition, the comparative investigation of different DNA methylation modifications is novel and disproves a direct role in var gene regulation.

Reviewed by eLife

Quantitative comparison of PI(3,5)P ₂ biosensors reveals SnxA is the most sensitive and unbiased

1. Tiernan Swayhoover
2. Claire C. Weckerly
3. Gerald R. V. Hammond

Reviewed by Review Commons

Cell loss disrupts mechanical homeostasis to drive retinal pigment epithelium ageing-like phenotype in vitro

1. Teodora Piskova
2. Aleksandra N. Kozyrina
3. Giedrė Astrauskaitė
4. Mohamed Elsafi Mabrouk
5. Sebastian Schepl
6. Stacy Lok Sze Yam
7. Ragul Ravithas
8. Wolfgang Wagner
9. Massimo Vassalli
10. Jacopo Di Russo

Reviewed by preLights

CD8 T lymphocytes redeploy embryonic cell cycle control mechanisms to facilitate rapid cell proliferation

1. D. A. Lewis
2. A. Kar
3. A. Savage
4. V. Kelly
5. D. Wright
6. R. Zamoyska
7. T. Ly

Reviewed by Review Commons

Contractile peri-nuclear actomyosin network repositions peripheral and polar chromosomes to promote early kinetochore–microtubule interactions

1. Nooshin Sheidaei
2. John K Eykelenboom
3. Zuojun Yue
4. Graeme Ball
5. Alexander JR Booth
6. Tomoyuki U Tanaka

• Curated by eLife

  eLife Assessment

  This important study demonstrates that a peri-nuclear actomyosin network, present in some types of human cells, facilitates kinetochore-spindle attachment of chromosomes in unfavorable locations - thereby suppressing their missegregation rate. This actomyosin network and its general role have been studied previously, but this study convincingly clarifies the underlying mechanism using a light-controlled perturbation and detailed tracking of kinetochore movement. The generality of the mechanism could be further supported by confirming the findings in non-synchronized cells and additional cell lines. The results may have implications for understanding chromosome missegregation in cancer cells.

  [Editors' note: this paper was reviewed by Review Commons.]

Reviewed by eLife, Review Commons

Progenitor identification and SARS-CoV-2 infection in long-term human distal lung organoid cultures

1. Ameen A. Salahudeen
2. Shannon S. Choi
3. Arjun Rustagi
4. Junjie Zhu
5. Sean M. de la O
6. Ryan A. Flynn
7. Mar Margalef-Català
8. António J. M. Santos
9. Jihang Ju
10. Arpit Batish
11. Vincent van Unen
12. Tatsuya Usui
13. Grace X.Y. Zheng
14. Caitlin E. Edwards
15. Lisa E. Wagar
16. Vincent Luca
17. Benedict Anchang
18. Monica Nagendran
19. Khanh Nguyen
20. Daniel J. Hart
21. Jessica M. Terry
22. Phillip Belgrader
23. Solongo B. Ziraldo
24. Tarjei S. Mikkelsen
25. Pehr B. Harbury
26. Jeffrey S. Glenn
27. K. Christopher Garcia
28. Mark M. Davis
29. Ralph S. Baric
30. Chiara Sabatti
31. Manuel R. Amieva
32. Catherine A. Blish
33. Tushar J. Desai
34. Calvin J. Kuo

Reviewed by Rapid Reviews Infectious Diseases, PREreview, ScreenIT

Unconventional components complement the cryptic kinetochore of the ciliate Tetrahymena thermophila

1. Emine I. Ali
2. Maximilian W. D. Raas
3. Laura E. van Rooijen
4. Paula Sobrevals Alcaraz
5. Harmjan R. Vos
6. Eelco C. Tromer
7. Berend Snel
8. Geert J.P.L. Kops

Reviewed by Review Commons

Proteome dynamics reveal Leiomodin 1 as a key regulator of myogenic differentiation

1. Ellen Späth
2. Svenja C Schüler
3. Ivonne Heinze
4. Therese Dau
5. Alberto Minetti
6. Maleen Hofmann
7. Katja Hönzke
8. Julia von Maltzahn
9. Alessandro Ori

• Curated by eLife

  eLife Assessment

  This study provides valuable insights into the regulation of myogenic differentiation by identifying Leiomodin 1 as a modulator of proteome dynamics during myogenic differentiation. The combination of quantitative proteomics with functional perturbation experiments offers solid evidence supporting the idea that SIRT1 influences perturbations of myogenic differentiation upon LMOD1 inactivation. These findings advance our understanding of muscle differentiation and will be of interest to researchers studying muscle development and related pathologies

Reviewed by eLife