Cell Biology

Overexpression of Ssd1 and calorie restriction extend yeast replicative lifespan by preventing deleterious age-dependent iron uptake

1. J Ignacio Gutierrez
2. Claudia Edgar
3. Jessica K Tyler

• Curated by eLife

  eLife Assessment

  This important study uses innovative microfluidics-based single-cell imaging to monitor replicative lifespan, protein localization, and intracellular iron levels in aging yeast cells. The evidence for the proposed role of Ssd1 and reduced nutrients for lifespan through limiting iron uptake is convincing, even though some mechanistic details remain unclear. This work will be of interest to cell biologists working on aging and iron metabolism.

Reviewed by eLife

A transcriptionally active lipid vesicle encloses the injected Chimalliviridae genome in early infection

1. Emily G. Armbruster
2. Phoolwanti Rani
3. Jina Lee
4. Niklas Klusch
5. Joshua Hutchings
6. Lizbeth Y. Hoffman
7. Hannah Buschkaemper
8. Eray Enustun
9. Benjamin A. Adler
10. Koe Inlow
11. Arica R. VanderWal
12. Madelynn Y. Hoffman
13. Daksh Daksh
14. Ann Aindow
15. Amar Deep
16. Zaida K. Rodriguez
17. Chase J. Morgan
18. Majid Ghassemian
19. Thomas G. Laughlin
20. Emeric Charles
21. Brady F. Cress
22. David F. Savage
23. Jennifer A. Doudna
24. Kit Pogliano
25. Kevin D. Corbett
26. Elizabeth Villa
27. Joe Pogliano

Reviewed by Rapid Reviews Infectious Diseases, Arcadia Science

Histone methyltransferase DOT1L differentially affects the development of dendritic cell subsets

1. Rianne G. Bouma
2. Willem-Jan de Leeuw
3. Aru Z. Wang
4. Muddassir Malik
5. Joeke G.C. Stolwijk
6. Veronique A.L. Konijn
7. Anne Mensink
8. Natalie Proost
9. Maarten K. Nijen Twilhaar
10. Tibor van Welsem
11. Negisa Seyed Toutounchi
12. Alsya J. Affandi
13. Jip T. van Dinter
14. Fred van Leeuwen
15. Joke M.M. den Haan

Reviewed by Review Commons

Endosome-associated Rab GTPases control distinct aspects of neural circuit assembly

1. Katherine X Dong
2. Hui Ji
3. David J Luginbuhl
4. Liqun Luo
5. Colleen N McLaughlin

• Curated by eLife

  eLife Assessment

  Dong et al. present a valuable analysis of mutant phenotypes of the Rab GTPases Rab5, Rab7, and Rab11 in Drosophila second-order olfactory neuron development. This is a solid characterization and comparison of the different Rab mutants on projection neuron development, with clear differences for the three Rabs, and by inference for the early, late, and recycling endosomal functions executed by each.

Reviewed by eLife

CIP2A Mediates the Recruitment of the SLX4-MUS81-XPF Tri-Nuclease Complex in Mitosis and Protects Against Replication Stress

1. Alice Meroni
2. Annica Pellizzari
3. Andrea Hänel
4. Nathalie Varisco
5. Pascale Brasier-Lutz
6. Isabell Witzel
7. Alessandro A Sartori
8. Manuel Stucki

Reviewed by Review Commons

Branched actin polymerization drives invasive protrusion formation to promote myoblast fusion during skeletal muscle regeneration

1. Yue Lu
2. Tezin Walji
3. Pratima Pandey
4. Chuanli Zhou
5. Christa W Habela
6. Scott B Snapper
7. Rong Li
8. Elizabeth H Chen

• Curated by eLife

  eLife Assessment

  This study presents a valuable finding regarding the role of Arp2/3 and the actin nucleators N-WASP and WAVE complexes in myoblast fusion. The data presented is convincing, and the work will be of interest to biologists studying skeletal muscle stem cell biology in the context of skeletal muscle regeneration.

Reviewed by eLife

Analysis of cancer mutations introduced into the Drosophila Notch Negative Regulatory Region uncovers a diversity of regulatory outcomes

1. Hideyuki Shimizu
2. Martin Baron

• Curated by eLife

  eLife Assessment

  This important study presents a technically rigorous and carefully controlled analysis of the signalling potential of cancer-associated gain-of-function Notch alleles. The work is clearly presented, and the experiments are robust, comprehensive, and well-controlled. While some data primarily establish the system or report negative findings, the comparative approach in a well-characterized model provides convincing mechanistic evidence for how these Notch variants function. This study will be of interest to researchers in both developmental and cancer biology.

Reviewed by eLife

A green lifetime biosensor for calcium that remains bright over its full dynamic range

1. Franka H van der Linden
2. Stephen C Thornquist
3. Rick M ter Beek
4. Jelle Y Huijts
5. Mark A Hink
6. Theodorus WJ Gadella
7. Gaby Maimon
8. Joachim Goedhart

• Curated by eLife

  eLife Assessment

  This manuscript reports on an FLIM-based calcium biosensor, G-CaFLITS. It represents an important contribution to the field of genetically-encoded fluorescent biosensors, and will serve as a practical tool for the FLIM imaging community. The paper provides convincing evidence of G-CaFLITS's photophysical properties and its advantages over previous biosensors such as Tq-Ca-FLITS. Although the benefits of G-Ca-FLITS over Tq-Ca-FLITS are limited by the relatively small wavelength shift, it presents some advantages in terms of compatibility with available instrumentation and brightness consistency.

Reviewed by eLife

NudC moonlights in ribosome biogenesis and homeostasis in Drosophila melanogaster polyploid cells

1. Duoduo Shi
2. Yuko Shimada-Niwa
3. Naoki Okamoto
4. Akira Nakamura
5. Yuya Ohhara
6. Wei Sun
7. Ryusuke Niwa

Reviewed by Review Commons

Ciliary and non-ciliary functions of CEP104 in Xenopus

1. Panagiota Louka
2. Louiza Potamiti
3. Mihalis I. Panayiotidis
4. Paris Skourides

Reviewed by Review Commons