Cell Biology

Synergistic effects of deleting the tyrosine phosphatases Shp1 and Shp2 on megakaryopoiesis and thrombopoiesis in mice

1. Elsa Barré
2. Marc-Damien Lourenco-Rodrigues
3. Lucie Zimmermann
4. Marion Pugliano
5. Cécile Loubière
6. Fabienne Proamer
7. Jean-Yves Rinckel
8. Anita Eckly
9. Zihan Qu
10. Jinmin Miao
11. Zhong-Yin Zhang
12. Yotis A Senis
13. Alexandra Mazharian

• Curated by eLife

  eLife assessment

  This manuscript provides an important contribution to the field of platelet biogenesis, and the convincing evidence will advance our understanding of signal transduction driving the development of late megakaryopoiesis and platelet reactivity that results in bleeding diathesis. The paper is noteworthy for analyzing two related, either singly or in combination, tyrosine phosphatases in this conditional, stage development gene knockout. Because SHP1 is a negative regulator and SHP2 is an activator, the synergistic effects found in the double knockout were surprising.

Reviewed by eLife

Structural insights into mitotic-centrosome assembly

1. Nada Mohamad
2. Siu-Shing Wong
3. Anupa Majumdar
4. Alan Wainman
5. Ingelise Holland-Kaye
6. Lars Hubatsch
7. Zsofia Novak
8. Andrey Pozniakovsky
9. Martine Ruer-Gruss
10. Andreas F.M. Haensele
11. Anna Caballe
12. Steven Johnson
13. Susan M. Lea
14. Anthony Hyman
15. Jordan W. Raff

Reviewed by Review Commons

ATG2A engages RAB1A and ARFGAP1 positive membranes during autophagosome biogenesis

1. Devin M Fuller
2. Yumei Wu
3. Florian Schueder
4. Burha Rasool
5. Shanta Nag
6. Justin L Korfhage
7. Rolando Garcia-Milian
8. Katerina D Melnyk
9. Joerg Bewersdorf
10. Pietro De Camilli
11. Thomas J Melia

• Curated by eLife

  eLife Assessment

  This study provides important insights into the crosstalk between ATG2A with components of the early secretory pathway. While the mechanisms governing autophagic membrane expansion remain yet to be fully understood, in this study the authors employ an elegant proximity labelling approach and identify two ER-Golgi intermediate compartment (ERGIC)-localized proteins. Through a series of complementary experiments combining microscopy and biochemistry, the authors identify ARFGAP1 and Rab1A as components of early autophagic membranes, which accumulate at the periphery of pre-autophagosomal structures induced by loss of ATG2. The overall study is well executed and the evidence supporting the claims is convincing.

Reviewed by eLife

Clathrin-independent endocytosis and retrograde transport in cancer cells tune immune synapse organization and CD8 T cell response

1. Shiqiang Xu
2. Alix Buridant
3. Thibault Hirsch
4. Céline Duhamel
5. Benjamin Ledoux
6. Massiullah Shafaq-Zadah
7. Estelle Dransart
8. Louise Thines
9. Ludger Johannes
10. Pierre Van der Bruggen
11. Pierre Morsomme
12. Henri-François Renard

• Curated by eLife

  eLife Assessment

  This study now provides solid evidence for a role of EndoA3-mediated trafficking of ICAM-1 to the immune synapse with T cells. The study will be valuable to those studying cell-cell communication in the immune system, and opens additional questions regarding the mechanisms involved and how other adhesion ligands are regulated.

Reviewed by eLife

GTPase-activating protein DLC1 spatio-temporally regulates Rho signaling

1. Lucien Hinderling
2. Max Heydasch
3. Giliane Rochat
4. Laurent Dubied
5. Jakobus van Unen
6. Maciej Dobrzynski
7. Olivier Pertz

• Curated by eLife

  eLife Assessment

  This study presents a valuable finding on how the GAP DLC1, a deactivator of the small GTPase RhoA, regulates RhoA activity globally as well as at Focal Adhesions. Using a new acute optogenetic system coupled to a RhoA activity biosensor, the authors present convincing evidence that DLC1 amplifies local Rho activity at Focal Adhesions. Thanks to modeling, they show that DLC1 is needed for a negative feedback loop that engage more RhoA deactivators upon RhoA activation, highlighting the complex regulation of RhoGTPases in space and time.

Reviewed by eLife

Single-cell lineage tracing identifies hemogenic endothelial cells in the adult mouse bone marrow

1. Jing-Xin Feng
2. Mei-Ting Yang
3. Lili Li
4. Caiyi C Li
5. Ferenc Livak
6. Jack Chen
7. Yongmei Zhao
8. Dunrui Wang
9. Avinash Bhandoola
10. Naomi Taylor
11. Giovanna Tosato

• Curated by eLife

  eLife Assessment

  This manuscript by Feng et al. provides valuable evidence regarding the hematopoietic differentiation of bone marrow endothelial cells in the adult mouse. Overall, the authors have addressed our main concerns. Solid data now more strongly support long-term multi-lineage reconstitution of the adult hemogenic endothelial cells. However, critical data, especially regarding the endothelial cells' hematopoietic identity and functional capacity, remain insufficient, which limits the strength of the hemogenic claim, especially the assertion that these adult hemogenic ECs generate bona fide HSCs. Additional experiments would be necessary to fully rule out alternative explanations.

Reviewed by eLife

Epigenetics and chromatin structure regulate var2csa expression and the placental-binding phenotype in Plasmodium falciparum

1. Todd Lenz
2. Madle Sirel
3. Hannes Hoppe
4. Sulman Shafeeq
5. Karine G Le Roch
6. Ulf Ribacke

• Curated by eLife

  eLife Assessment

  This interesting study presents a multi-OMICs approach to unify different lines of evidence regarding the epigenetic regulation of the key virulence factor causing placental malaria during P. falciparum infection. Most results are confirmatory of previous observations; nonetheless, the claims are supported by convincing evidence. The combinatorial approach chosen here is unprecedented and therefore provides valuable new data. In addition, the comparative investigation of different DNA methylation modifications is novel and disproves a direct role in var gene regulation.

Reviewed by eLife

Quantitative comparison of PI(3,5)P ₂ biosensors reveals SnxA is the most sensitive and unbiased

1. Tiernan Swayhoover
2. Claire C. Weckerly
3. Gerald R. V. Hammond

Reviewed by Review Commons

Cell loss disrupts mechanical homeostasis to drive retinal pigment epithelium ageing-like phenotype in vitro

1. Teodora Piskova
2. Aleksandra N. Kozyrina
3. Giedrė Astrauskaitė
4. Mohamed Elsafi Mabrouk
5. Sebastian Schepl
6. Stacy Lok Sze Yam
7. Ragul Ravithas
8. Wolfgang Wagner
9. Massimo Vassalli
10. Jacopo Di Russo

Reviewed by preLights

CD8 T lymphocytes redeploy embryonic cell cycle control mechanisms to facilitate rapid cell proliferation

1. D. A. Lewis
2. A. Kar
3. A. Savage
4. V. Kelly
5. D. Wright
6. R. Zamoyska
7. T. Ly

Reviewed by Review Commons