Cell Biology

The insulin / IGF axis is critically important for controlling gene transcription in the podocyte

1. Jennifer A Hurcombe
2. Lusyan Dayalan
3. Fern Barrington
4. Frédéric Burdet
5. Lan Ni
6. Joseph T Coward
7. Mark Ibberson
8. Paul T Brinkkoetter
9. Martin Holzenberger
10. Aaron Jeffries
11. Sebastian Oltean
12. Gavin I Welsh
13. Richard JM Coward

• Curated by eLife

  eLife Assessment

  This valuable study used genetic and pharmacological manipulations of insulin/IGF signaling to address the role of insulin/IGF axis in the function of renal glomerular podocyte. Solid data are presented to demonstrate that co-inhibition of insulin/IGF signaling in podocytes led to aberrant splicing of mRNAs, which could contribute to the loss of podocytes in vitro and in vivo in mice. In light of the fact that IR/IGF-1R signaling are critically required for normal development and growth in multiple cells and organs, the lack of the assessment of developmental phenotype of podocytes in the mouse model limits the interpretation of the data.

  [Editors' note: this paper was reviewed by Review Commons.]

Reviewed by eLife

Peripheral lysosomes recruit PLEKHG3 to focal adhesions and restrain protrusion dynamics

1. Rainer Ettelt
2. Ana-Maria Sandru
3. Georg Vucak
4. Sebastian Didusch
5. Biljana Riemelmoser
6. Karin Ehrenreiter
7. Markus Hartl
8. Lukas A. Huber
9. Manuela Baccarini

Reviewed by Review Commons

Muscular dystrophy-associated lamin variants disrupt cellular organization through a nucleolar-ribosomal axis controlling cytoplasmic macromolecular crowding

1. Xiangyi Ding
2. Sweta Kumari
3. Ellen Gregory
4. Daniel A. Starr
5. G. W. Gant Luxton

Reviewed by Arcadia Science

Syntaxin11 Deficiency Inhibits CRAC Channel Priming to Suppress Cytotoxicity and Gene Expression in T Lymphocytes

1. Sritama Datta
2. Abhikarsh Gupta
3. Kunal Mukesh Jagetiya
4. Resmi Bera
5. Vikas Tiwari
6. Atharva Rahul Yande
7. Megumi Yamashita
8. Abdul Rishad
9. Vishal Malik
10. Sreejith Raran-Kurussi
11. Sandra Ammann
12. Mohammad Shahrooei
13. Kalyaneswar Mandal
14. Ramanathan Sowdhamini
15. Murali Prakriya
16. Adish Dani
17. Monika Vig

• Curated by eLife

  eLife Assessment

  This study reports a novel function for syntaxin 11, a specialized SNARE protein critical for the immune system whose mutations cause familial hemophagocytic lymphohistiocytosis type 4. The data convincingly show that depletion of STX11 impairs store-operated calcium entry in Jurkat T cells and that this defect is recapitulated in primary cells from a patient suffering from the disease; the authors further show that the syntaxin interacts with the pore subunit of the ORAI1 channel and propose that it primes the channel by promoting the assembly of multimers before activation by its endogenous ligand, the ER Ca2+ sensing protein STIM1. This is a conceptually important claim that challenges the prevailing view that all structural transitions in ORAI1 are STIM-driven. The data are high-quality and broadly consistent with the interpretation, but alternative mechanisms for the defects are not considered; additional work should rule out vesicular trafficking, discuss other mechanisms, and address methodological issues.

Reviewed by eLife

The functional maturation of mouse spermatozoa is underpinned by global remodeling of the cellular phosphoproteome

1. David A. Skerrett-Byrne
2. Amanda L. Anderson
3. Raffaele Teperino
4. Nathan D. Burke
5. Elizabeth G. Bromfield
6. Matthew D. Dun
7. Valérie Gailus-Durner
8. Helmut Fuchs
9. Susan Marschall
10. Martin Hrabě de Angelis
11. Sean J. Humphrey
12. Brett Nixon

Reviewed by Review Commons