Cell Biology

Enteropathogenic Escherichia coli-mediated fast and coordinated Ca²+ responses regulate NF-κB activation

1. Fangrui Guo
2. Roberto Ornelas Guevara
3. Linda Oussaedine
4. Geneviève Dupont
5. Laurent Combettes
6. Guy Tran Van Nhieu

• Curated by eLife

  eLife Assessment

  This study reports important advances in our understanding of how enteropathogenic E. coli (EPEC) interacts at the intestinal interface. Compelling data describe a novel model of spatially coordinated calcium signaling to modulate NF-kB activation. These findings, which integrate imaging, genetics, and computational modeling, provide a new way to consider host-pathogen interactions in EPEC infections that may lead to improved therapies.

Reviewed by eLife

Peripheral lysosomes recruit PLEKHG3 to focal adhesions and restrain protrusion dynamics

1. Rainer Ettelt
2. Ana-Maria Sandru
3. Georg Vucak
4. Sebastian Didusch
5. Biljana Riemelmoser
6. Karin Ehrenreiter
7. Markus Hartl
8. Lukas A. Huber
9. Manuela Baccarini

Reviewed by Review Commons

Contractile perinuclear actomyosin network promotes peripheral and polar chromosome interaction with the mitotic spindle

1. Nooshin Sheidaei
2. John K Eykelenboom
3. Zuojun Yue
4. Graeme Ball
5. Alexander JR Booth
6. Tomoyuki U Tanaka

• Curated by eLife

  eLife Assessment

  This important study demonstrates that a perinuclear actomyosin network, present in some types of human cells, facilitates kinetochore-spindle attachment of chromosomes in unfavorable locations, thereby reducing their missegregation rate. This actomyosin network and its general role have been studied previously, but this study convincingly clarifies the underlying mechanism and expands the investigation to additional cell lines. The results are relevant to understanding chromosome missegregation in cancer cells.

  [Editors' note: this paper was reviewed by Review Commons.]

Reviewed by eLife, Review Commons

Muscular dystrophy-associated lamin variants disrupt cellular organization through a nucleolar-ribosomal axis controlling cytoplasmic macromolecular crowding

1. Xiangyi Ding
2. Sweta Kumari
3. Ellen Gregory
4. Daniel A. Starr
5. G. W. Gant Luxton

Reviewed by Arcadia Science

Non-canonical amino acid incorporation enables minimally disruptive labeling of stress granule and TDP-43 proteinopathy

1. Hao Chen
2. Haocheng Wang
3. Yu-Ning Lu
4. Peng Chen
5. Zhongfan Zheng
6. Tao Zhang
7. Jiou Wang

• Curated by eLife

  eLife Assessment

  This paper demonstrates that a genetic code expansion to tag two amyotrophic lateral sclerosis (ALS) proteins associated with stress granules is useful in an experimental context. The data are solid and demonstrate the feasibility of using ANAP-fluorescence for live cell imaging.

Reviewed by eLife

Syntaxin11 Deficiency Inhibits CRAC Channel Priming to Suppress Cytotoxicity and Gene Expression in T Lymphocytes

1. Sritama Datta
2. Abhikarsh Gupta
3. Kunal Mukesh Jagetiya
4. Resmi Bera
5. Vikas Tiwari
6. Atharva Rahul Yande
7. Megumi Yamashita
8. Abdul Rishad
9. Vishal Malik
10. Sreejith Raran-Kurussi
11. Sandra Ammann
12. Mohammad Shahrooei
13. Kalyaneswar Mandal
14. Ramanathan Sowdhamini
15. Murali Prakriya
16. Adish Dani
17. Monika Vig

• Curated by eLife

  eLife Assessment

  This study reports a novel function for syntaxin 11, a specialized SNARE protein critical for the immune system whose mutations cause familial hemophagocytic lymphohistiocytosis type 4. The data convincingly show that depletion of STX11 impairs store-operated calcium entry in Jurkat T cells and that this defect is recapitulated in primary cells from a patient suffering from the disease; the authors further show that the syntaxin interacts with the pore subunit of the ORAI1 channel and propose that it primes the channel by promoting the assembly of multimers before activation by its endogenous ligand, the ER Ca2+ sensing protein STIM1. This is a conceptually important claim that challenges the prevailing view that all structural transitions in ORAI1 are STIM-driven. The data are high-quality and broadly consistent with the interpretation, but alternative mechanisms for the defects are not considered; additional work should rule out vesicular trafficking, discuss other mechanisms, and address methodological issues.

Reviewed by eLife

The functional maturation of mouse spermatozoa is underpinned by global remodeling of the cellular phosphoproteome

1. David A. Skerrett-Byrne
2. Amanda L. Anderson
3. Raffaele Teperino
4. Nathan D. Burke
5. Elizabeth G. Bromfield
6. Matthew D. Dun
7. Valérie Gailus-Durner
8. Helmut Fuchs
9. Susan Marschall
10. Martin Hrabě de Angelis
11. Sean J. Humphrey
12. Brett Nixon

Reviewed by Review Commons

Cell size modulates ferroptosis susceptibility

1. Evgeny Zatulovskiy
2. Magdalena B Murray
3. Shuyuan Zhang
4. Scott J Dixon
5. Jan M Skotheim

• Curated by eLife

  eLife Assessment

  This important study highlights how cell size influences various cellular responses, with a particular focus on ferroptosis. The evidence presented is convincing, employing multiple model systems and experimental approaches to support the conclusions. This work will be of significant interest to the fields of cell size, ferroptosis, and cancer biology.

  [Editors' note: this paper was reviewed by Review Commons.]

Reviewed by eLife, Review Commons

Fibroblast alignment coordinates epithelial migration and maintains intestinal tissue integrity

1. Jordi Comelles
2. Aina Abad-Lázaro
3. Verónica Acevedo
4. David Bartolomé-Català
5. Aitor Otero-Tarrazón
6. Anna Esteve-Codina
7. Xavier Hernando-Momblona
8. Eduard Batlle
9. Vanesa Fernández-Majada
10. Elena Martinez

Reviewed by Review Commons

Analysis of cancer mutations introduced into the Drosophila melanogaster Notch negative regulatory region uncovers a diversity of regulatory outcomes

1. Hideyuki Shimizu
2. Martin Baron

• Curated by eLife

  eLife Assessment

  This important study presents a technically rigorous and carefully controlled analysis of the signalling potential of cancer-associated gain-of-function Notch alleles. The work is clearly presented, and the experiments are robust, comprehensive, and well-controlled. While some data primarily establish the system or report negative findings, the comparative approach in a well-characterized model provides convincing mechanistic evidence for how these Notch variants function. This study will be of interest to researchers in both developmental and cancer biology.

Reviewed by eLife