Cell Biology

Analysis of cancer mutations introduced into the Drosophila Notch Negative Regulatory Region uncovers a diversity of regulatory outcomes

1. Hideyuki Shimizu
2. Martin Baron

• Curated by eLife

  eLife Assessment

  This important study presents a technically rigorous and carefully controlled analysis of the signalling potential of cancer-associated gain-of-function Notch alleles. The work is clearly presented, and the experiments are robust, comprehensive, and well-controlled. While some data primarily establish the system or report negative findings, the comparative approach in a well-characterized model provides convincing mechanistic evidence for how these Notch variants function. This study will be of interest to researchers in both developmental and cancer biology.

Reviewed by eLife

An ancient transcription factor functions as the master regulator of primary cilia formation

1. Weihua Wang
2. Xiqi Zhang
3. Yaxuan Qiu
4. Xiangrui Meng
5. Sitong Cheng
6. Yutong Chen
7. Siqi Liu
8. Wenhui Chen
9. Jiayan Yi
10. Xiwen You
11. Hongni Liu
12. Junqiao Xing
13. Cheng Xu
14. Haochen Jiang
15. Haibo Wang
16. Guangmei Tian
17. Zhangfeng Hu

• Curated by eLife

  eLife Assessment

  This useful study identified XAP5 as an ancient transcriptional regulator critical for primary ciliogenesis. The evidence supporting the conceptual framework linking evolutionary conservation to functional specialization in primary ciliogenesis remains incomplete. This work will be of interest to developmental biologists and to those studying diseases caused by ciliopathies.

Reviewed by eLife

Spatiotemporal dynamics of DNA repair proteins between the Golgi and nucleus maintain genomic stability

1. George Galea
2. Karolina Kuodyte
3. Muzamil M. Khan
4. Peter Thul
5. Beate Neumann
6. Emma Lundberg
7. Rainer Pepperkok

Reviewed by Review Commons

MORC2 mediates transcriptional regulation through liquid-liquid phase separation

1. Yanshen Zhang
2. Weiya Xu
3. Wenxiu Duan
4. Yu Wei
5. Wenli Jiang
6. Feng Zhu
7. Chengdong Huang
8. Chao Wang
9. Yihui Bi

• Curated by eLife

  eLife Assessment

  This important study has demonstrated that MORC2 undergoes phase separation in cells and established multiple interactions responsible for the phase separation. Upon revision, the data generally provide solid support to the claim that MORC2 condensates are functionally relevant in gene regulation and begins to demonstrate the importance of the physical properties of biological condensates. Nevertheless, there remains some weakness in the connection between condensates and function.

Reviewed by eLife

The Arp2/3 complex maintains genome integrity and survival of epidermal Langerhans cells

1. Maria-Graciela Delgado
2. Ann-Kathrin Burkhart
3. Charlotte Canet-Jourdan
4. Javiera Villar
5. Roberto Amadio
6. Vanessa S. Racz
7. Doriane Sanséau
8. Nilushi De Silva
9. Livia Lacerda Mariano
10. Anna Shmakova
11. Anna Chipont
12. Mabel San Roman
13. Giulia Bracchetti
14. Vincent Calmettes
15. Zahraa Alraies
16. Mathieu Maurin
17. Aurelien Dauphin
18. Coralie Guerin
19. Florent Ginhoux
20. Nicolas Manel
21. Daniele Fachinetti
22. Federica Benvenuti
23. Ana-Maria Lennon-Duménil
24. Sandra Iden

Reviewed by Review Commons

Application of nucleoside analogue labelling to study the cell cycle of xenografted PDAC cell lines in the chorioallantoic membrane model

1. Robin Colenbier
2. Denisa Lodoaba Jurescu
3. Jean-Pierre Timmermans
4. Johannes Bogers

Reviewed by Review Commons

LRRK2 mutations block NCOA4 trafficking upon iron overload leading to ferroptotic death

1. Andres Goldman
2. Mai Nguyen
3. Joel Lanoix
4. Chongyang Li
5. Ahmed Fahmy
6. Yong Zhong Xu
7. Erwin Schurr
8. Pierre Thibault
9. Michel Desjardins
10. Heidi M. McBride

Reviewed by Review Commons

CD90 identifies distinct fractions of muscle stem cells with different modalities of activation and quiescence maintenance

1. Eyemen Kheir
2. Michela Libergoli
3. Samuele Metti
4. Jonas Brorson Jensen
5. Jakob Wang
6. Niels Jessen
7. Francesca Florio
8. Francesca Murganti
9. Serena Di Savino
10. Marzia Belicchi
11. Chiara Villa
12. Kristian Vissing
13. Mayank Verma
14. Lorenzo Giordani
15. Paolo Bonaldo
16. Yvan Torrente
17. Jean Farup
18. Stefano Biressi

Reviewed by Review Commons

Hsp70 is phosphorylated in a conserved response to DNA damage and contributes to cell cycle control

1. Thomas Moss
2. Alexandra Wooldredge
3. Koustav Bhakta
4. Matthew Cronin
5. Jason E Gestwicki
6. Shaeri Mukherjee

• Curated by eLife

  eLife Assessment

  This potentially valuable manuscript focuses on the phosphorylation of residue T495 as a mechanism to inactivate HSP70 and disrupt cell cycle progression in response to DNA damage. The evidence supporting this model is solid, but would be significantly strengthened by additional studies defining the extent of T495 phosphorylation induced by DNA damage, identifying the kinase responsible for phosphorylating T495 of HSP70, and further elucidation of the functional implications of T495 phosphorylation in human cells. This work will be of interest to scientists focused on topics including chaperone biology, proteostasis, cell cycle progression, and DNA damage.

Reviewed by eLife, preLights

Capsaicin acts as a novel NRF2 agonist to suppress ethanol induced gastric mucosa oxidative damage by directly disrupting the KEAP1-NRF2 interaction

1. Xiaoning Gao
2. Wuyan Guo
3. Peiyuan Liu
4. Mingyue Yuwen
5. Hongyu Ren
6. Shengtao Hu
7. Zixiang Liu
8. Ruyang Tan
9. Kairui Liu
10. Zhiru Yang
11. Junli Ba
12. Xue Bai
13. Shiti Shama
14. Cong Tang
15. Kai Miao
16. Haozhi Pei
17. Liren Liu
18. Cheng Zhu
19. Tao Wang
20. Bo Zhang
21. Jun Kang

• Curated by eLife

  eLife Assessment

  This valuable study suggests that capsaicin nanoparticle administration in rats activates the transcription factor Nrf2 by directly binding to its repressor, KEAP1, leading to the induction of cytoprotective genes and preventing alcohol-induced gastric damage, offering a potential avenue for treating alcoholism-related gastric disorders. The authors provide solid evidence through a wealth of biochemical experiments in vitro, in cultured cells as well as in a rat model. The work will be of great interest to researchers studying oxidative damage in a variety of different diseases and the exploitation of molecules for therapeutic approaches.

Reviewed by eLife