Cell Biology

The Rho effector ARHGAP18 coordinates a Hippo pathway feedback loop through YAP and Merlin to regulate the cytoskeleton and epithelial cell polarity

1. Emma C. Murray
2. Gillian M. Hodge
3. Khanh Pham
4. Leighton S. Lee
5. Cameron A.R. Mitchell
6. Yongho Bae
7. Andrew T. Lombardo

Reviewed by Review Commons

Centrosome Loss in Embryonic Development Disrupts Axonal Pathfinding and Muscle Integrity

1. Beatriz González
2. Júlia Sellés-Altés
3. Jèrica Pla-Parron
4. Judith Castro-Ribera
5. Sofia J. Araújo

Reviewed by Review Commons

MAP7 directs apical cytoskeletal remodeling to establish Sertoli cell polarity and support germ cell development

1. Koji Kikuchi
2. Toshihiko Fujimori
3. Mami Nakagawa
4. Keisuke Ohta
5. Ryuki Shimada
6. Sayoko Fujimura
7. Kei-ichiro Yasunaga
8. Shingo Usuki
9. Naoki Tani
10. Akira Nakamura
11. Kimi Araki
12. Kei-ichiro Ishiguro

Reviewed by Review Commons

The Rhizarian amoeba Filoreta ramosa develops a neuron-like arborized network using conserved cytoskeletal mechanisms

1. Sarah L. Guest
2. Scott C. Dawson

Reviewed by Arcadia Science

TRIM32 controls timely cell cycle exit in muscular differentiation through c-Myc down-regulation

1. Lu Xiong
2. Elisa Lazzari
3. Sabrina Pacor
4. Simeone Dal Monego
5. Erica Piovesan
6. Danilo Licastro
7. Germana Meroni

Reviewed by Review Commons

Cross-species evaluation of TANGO2 homologs, including HRG-9 and HRG-10 in Caenorhabditis elegans, challenges a proposed role in heme trafficking

1. Sarah E Sandkuhler
2. Kayla S Youngs
3. Olivia Gottipalli
4. Laura D Owlett
5. Monica B Bandora
6. Aaliya Naaz
7. Euri Kim
8. Lili Wang
9. Andrew Wojtovich
10. Vandana Gupta
11. Michael Sacher
12. Samuel J Mackenzie

• Curated by eLife

  eLife Assessment

  This valuable study provides solid evidence that supports TANGO2 homologs, including HRG-9 and HRG-10, can play a role in cellular bioenergetics and oxidative stress homeostasis. It also challenges the previously reported role of TANGO in heme transport and paves the way for future mechanistic studies addressing the mechanisms of how TANGO2 regulates oxidative stress homeostasis. The strengths include the use of different model systems, genetic tools, behavioral assays and efforts by the authors in using the same reagents to reproduce results of other groups.

Reviewed by eLife

THE FAM53C/DYRK1A axis regulates the G1/S transition of the cell cycle

1. Taylar Hammond
2. Jong Bin Choi
3. Miles W Membreño
4. Janos Demeter
5. Roy Ng
6. Debadrita Bhattacharya
7. Thuyen N Nguyen
8. Griffin G Hartmann
9. Caterina I Colón
10. Carine Bossard
11. Jan M Skotheim
12. Peter K Jackson
13. Anca Pasca
14. Seth M Rubin
15. Julien Sage

• Curated by eLife

  eLife Assessment

  This study identifies the uncharacterised protein FAM53C as a novel, potential regulator of the G1/S cell cycle transition, linking its function to the DYRK1A kinase and the RB/p53 pathways. The work is valuable and of interest to the cell cycle field, leveraging a strong computational screen to identify a new candidate. The findings are solid, although confidence in the siRNA depletion phenotypes would have been higher with rescue experiments using an siRNA-resistant cDNA and more robust quantification of some immunoassay data.

  [Editors' note: this paper was reviewed by Review Commons.]

Reviewed by eLife, Review Commons

Extracellular vesicle-mediated release of bis(monoacylglycerol)phosphate is regulated by LRRK2 and Glucocerebrosidase activity

1. Elsa Meneses-Salas
2. Moisés Castellá
3. Marianna Arnold
4. Frank Hsieh
5. Ruben Fernández-Santiago
6. Mario Ezquerra
7. Alicia Garrido
8. María-José Martí
9. Carlos Enrich
10. Suzanne R Pfeffer
11. Kalpana Merchant
12. Albert Lu

• Curated by eLife

  eLife Assessment

  This useful study presents the potentially interesting idea that LRRK2 regulates cellular BMP levels and their release via extracellular vesicles, with GCase activity further modulating this process in mutant LRRK2-expressing cells. However, some of the evidence supporting these conclusions remains incomplete, and additional work is suggested under certain conditions. Overall, the study will be of interest to cell biologists working on Parkinson's disease.

Reviewed by eLife

Adaptation of endothelial cells to microenvironment topographical cues through lysyl oxidase like-2-mediated basement membrane scaffolding

1. Marion F Marchand
2. Noémie Brassard-Jollive
3. Claire Leclech
4. Jorge Barrasa-Fano
5. Yoann Atlas
6. Claudia Umana-Diaz
7. Apeksha Shapeti
8. Corinne Ardidie-Robouant
9. Tristan Piolot
10. Sabrina Martin
11. Philippe Mailly
12. Christophe Guilluy
13. Abdul I Bakarat
14. Catherine Monnot
15. Hans Van Oosterwyck
16. Stéphane Germain
17. Laurent Muller

• Curated by eLife

  eLife Assessment

  This study presents important findings describing the early assembly of vascular basement membrane and how vascular cells switch from responding to cues provided by the external environment to those provided by self-assembled basement membrane. The evidence supporting the claims of the authors is convincing, with state-of-the-art microscopy and several different culture conditions examined. The work will be of interest to cell biologists studying the ECM, vascular development, as well as medical scientists focused on diseases that depend on vascular growth.

Reviewed by eLife

Noncanonical amino acid incorporation enables minimally disruptive labeling of stress granule and TDP-43 proteinopathy

1. Hao Chen
2. Haocheng Wang
3. Yuning Lu
4. Peng Chen
5. Zhongfan Zheng
6. Tao Zhang
7. Jiou Wang

• Curated by eLife

  eLife Assessment

  Amyotrophic lateral sclerosis (ALS) affects nerve cells in the brain and spinal cord. The authors' approach to use genetic code expansion to tag two ALS proteins associated with stress granules has value and should be useful in the ALS field. Parts of the work are well done, but there are concerns that the evidence is incomplete overall, and additional controls would strengthen the study.

Reviewed by eLife