Holotomographic microscopy reveals label-free quantitative dynamics of endothelial cells during endothelialization
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Abstract
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A
what is the box on the bottom right in A? Presumably it is the camera? A label would be helpful.
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Large-field tiled acquisitions highlight nuclei and clear cellboundaries, enabling the simultaneous observation of numerous cells.
how does this compare to just using standard brightfield imaging or other label-free techniques (e.g. DIC)? are there any features that quantitative phase picks up that other techniques miss?
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The precipitous drop in RI valuesmay be attributed to changes in cell density and volume as cells spread out,
Is it possible to determine computationally whether the drop in RI is only attributed to density change or something else?
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