Cell Biology

A high-throughput platform for single-molecule tracking identifies drug interaction and cellular mechanisms

1. David Trombley McSwiggen
2. Helen Liu
3. Ruensern Tan
4. Sebastia Agramunt Puig
5. Lakshmi B Akella
6. Russell Berman
7. Mason Bretan
8. Hanzhe Chen
9. Xavier Darzacq
10. Kelsey Ford
11. Ruth Godbey
12. Eric Gonzalez
13. Adi Hanuka
14. Alec Heckert
15. Jaclyn J Ho
16. Stephanie L Johnson
17. Reed Kelso
18. Aaron Klammer
19. Ruchira Krishnamurthy
20. Jifu Li
21. Kevin Lin
22. Brian Margolin
23. Patrick McNamara
24. Laurence Meyer
25. Sarah E Pierce
26. Akshay Sule
27. Connor Stashko
28. Yangzhong Tang
29. Daniel J Anderson
30. Hilary P Beck

• Curated by eLife

  eLife assessment

  This work presents an important technological advance, in the form of a high throughput platform for Single Particle Tracking allowing us to measure millions of cells and thousands of compounds per day. Analysis of the diffusional behaviour of fluorescently-tagged targets permits the identification of, and differentiation between, small molecules that bind directly or affect the target indirectly. The methodology and metrics employed are compelling, leading to the identification of multiple compounds that effectively change the diffusive state of the estrogen receptor, the POC target of the study.

Reviewed by eLife

Microtubule-dependent orchestration of centriole amplification in brain multiciliated cells

1. Amélie-Rose Boudjema
2. Rémi Balagué
3. Cayla E Jewett
4. Gina M LoMastro
5. Olivier Mercey
6. Adel Al Jord
7. Marion Faucourt
8. Alexandre Schaeffer
9. Camille Noûs
10. Nathalie Delgehyr
11. Andrew J Holland
12. Nathalie Spassky
13. Alice Meunier

• Curated by eLife

  eLife assessment

  In this important study, Boudjema et al. use cell culture models and advanced microscopic imaging to provide detailed analyses of the cellular events underlying centriole amplification, apical migration, and assembly of hundreds of motile cilia in multi-ciliated cells. This largely descriptive work provides a better understanding of this process that is of interest to cell biologists studying centrioles and cilia. Most of the claims are supported by the data, but the study would benefit from additional analyses regarding the roles of microtubules, which are currently incomplete, and from text editing to improve accessibility and readability, especially for a wider audience.

Reviewed by eLife

ESCRT disruption provides evidence against signaling functions for synaptic exosomes

1. Erica C Dresselhaus
2. Kathryn P Harris
3. Cassandra R R Blanchette
4. Kate Koles
5. Steven J Del Signore
6. Matthew F Pescosolido
7. Biljana Ermanoska
8. Mark Rozencwaig
9. Rebecca C Soslowsky
10. Michael J Parisi
11. Bryan A Stewart
12. Timothy J Mosca
13. Avital Adah Rodal

Reviewed by Review Commons

Plasmodium NEK1 coordinates MTOC organisation and kinetochore attachment during rapid mitosis in male gamete formation

1. Mohammad Zeeshan
2. Ravish Rashpa
3. David J. Ferguson
4. George Mckeown
5. Raushan Nugmanova
6. Amit K. Subudhi
7. Raphael Beyeler
8. Sarah L. Pashley
9. Robert Markus
10. Declan Brady
11. Magali Roques
12. Andrew R. Bottrill
13. Andrew M. Fry
14. Arnab Pain
15. Sue Vaughan
16. Anthony A. Holder
17. Eelco C. Tromer
18. Mathieu Brochet
19. Rita Tewari

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Gαi2 Interaction with EB1 Controls Microtubule Dynamics and Rac1 Activity in Xenopus Neural Crest Cell Migration

1. Soraya Villaseca
2. Juan Ignacio Leal
3. Lina Mariana Tovar
4. María José Ruiz
5. Jossef Guajardo
6. Hernan Morales-Navarrete
7. Roberto Mayor
8. Marcela Torrejón

Reviewed by Review Commons

PI3K/HSCB axis facilitates FOG1 nuclear translocation to promote erythropoiesis and megakaryopoiesis

1. Gang Liu
2. Yunxuan Hou
3. Xin Jin
4. Yixue Zhang
5. Chaoyue Sun
6. Chengquan Huang
7. Yujie Ren
8. Jianmin Gao
9. Xiuli Wang
10. Xiumei Jiang

• Curated by eLife

  eLife assessment

  This fundamental work significantly advances our understanding of how FOG1 nuclear localization is regulated during erythropoiesis and megakaryopoiesis, including the role of EPO and MPL/TPO signaling in this process. The authors provide compelling evidence using both K562 and CD34+ cells that heat shock cognate B (HSCB) can promote the proteasomal degradation of TACC3 to regulate the nuclear localization of FOG1, and that this function is independent of its role in iron-sulfur cluster (ISC) biogenesis. Together these data will be of interest to the fields of hematopoiesis and cell biology.

Reviewed by eLife

Novel 3D Approach to Model Non-Alcoholic Fatty Liver Disease using human Pluripotent Stem Cells

1. Carola Maria Morell
2. Samantha Grace Tilson
3. Rute Alexandra Tomaz
4. Arash Shahsavari
5. Andi Munteanu
6. Giovanni Canu
7. Brandon Tyler Wesley
8. Marion Perrin
9. Imbisaat Geti
10. Subhankar Mukhopadhyay
11. Francesca Mazzacuva
12. Paul Gissen
13. Jose Garcia-Bernardo
14. Martin Bachman
15. Casey Allison Rimland
16. Fotios Sampaziotis
17. Irina Mohorianu
18. Ludovic Vallier

• Curated by eLife

  eLife assessment

  The authors present an important study of a multi-cellular platform involving co-culturing of various hiPSC-derived hepatocyte like cells, cholangiocytes, stellate cells and macrophages to mimic the liver microenvironment. The aggregates are then treated with fatty acids and examined through transcriptomic and functional assays. The techniques and methodologically are sound, and the evidence supporting the conclusion is convincing, although more clinically relevant data demonstrating the effect of some potential pharmacological agents on the platform would serve to strengthen the study.

Reviewed by eLife

Transdifferentiation of fibroblasts into muscle cells to constitute cultured meat with tunable intramuscular fat deposition

1. Tongtong Ma
2. Ruimin Ren
3. Jianqi Lv
4. Ruipeng Yang
5. Xinyi Zheng
6. Yang Hu
7. Guiyu Zhu
8. Heng Wang

• Curated by eLife

  eLife assessment

  This study presents an important new technology for transdifferentiation of fibroblasts into muscle cells. The data and methods used for analysis were compelling. This study will have broad interest to cellular reprogramming biologists in particular as well as the general public.

Reviewed by eLife

Botulinum toxin intoxication requires retrograde transport and membrane translocation at the ER in RenVM neurons

1. Jeremy C Yeo
2. Felicia P Tay
3. Rebecca Bennion
4. Omar Loss
5. Jacquie Maignel
6. Laurent Pons
7. Keith Foster
8. Matthew Beard
9. Frederic Bard

• Curated by eLife

  eLife assessment

  In this valuable manuscript, Yeo et al. describe new methods for assessing the intracellular itinerary of Botulinum neurotoxin A (BoNT/A), a potent toxin used in clinical and cosmetic applications. The current manuscript challenges previously held views on how the catalytic portion of the toxin makes its way from the endocytic compartment to the cytosol, to meet its substrates. The approach taken is deemed innovative and the experiments are carefully performed, presenting solid evidence for some of the drawn conclusion; however, the conclusions one may draw from the experimental results are somewhat limited, as it is possible that the scope of their findings could be restricted to the specific neuron model and molecular tools that were used. This paper could be of interest to both cell biologists and physicians.

Reviewed by eLife

Partitioning to ordered membrane domains regulates the kinetics of secretory traffic

1. Ivan Castello-Serrano
2. Frederick A Heberle
3. Barbara Diaz-Rohrer
4. Rossana Ippolito
5. Carolyn R Shurer
6. Pablo Lujan
7. Felix Campelo
8. Kandice R Levental
9. Ilya Levental

• Curated by eLife

  eLife assessment

  In this important study, Castello-Serrano and colleagues describe, model and quantify the role of transmembrane domains in protein sorting in the secretory pathway, first at the ER and subsequently at the Golgi. Convincing data support the role of a cytoplasmic motif in ER exit, while further experiments are necessary to support a direct connection between the phase partitioning capability of the transmembrane regions and the sorting potential of domains at the Golgi/TGN.

Reviewed by eLife