Cell Biology

The eduWOSM: a benchtop advanced microscope for education and research

1. Nicholas J Carter
2. Douglas S Martin
3. Justin E Molloy
4. Robert A Cross

• Curated by eLife

  eLife Assessment

  This is a valuable study and a promising development for the field of open-source microscopy for educational purposes. The strengths include the low cost of constructing the microscope, impressive performance and detailed resources including a dedicated website and YouTube channel. The claims are generally supported by solid evidence, however, the manuscript would be strengthened by inclusion of further details on standard performance metrics (e.g. signal to noise ratio etc.) compared to existing systems and further details and clarification on the microscope, construction and operation.

Reviewed by eLife

p53 engagement is a hallmark of an unfolded protein response in the nucleus of mammalian cells

1. Joseph H Park
2. Thomas J Wandless

• Curated by eLife

  eLife Assessment

  This paper presents useful findings that misfolded proteins in the nucleus can impair proteasomal degradation and activate p53. The results supporting the findings are largely solid, but incomplete. The manuscript could be strengthened by including more quantitative data analyses and additional experimentation/discussions on the mechanism of p53 activation by misfolded nuclear proteins. The work will be interesting primarily to scientists studying protein homeostasis.

Reviewed by eLife

Adenomatous polyposis coli (APC) regulates internalization and signaling of the chemorepellent receptor, Roundabout (ROBO) 1

1. Yi-Wei Huang
2. Jonathan St-Germain
3. Bo Wen Pang
4. Richard F Collins
5. Etienne Coyaud
6. Wenjuan Li
7. Amir Mohamed
8. Brian Raught
9. Sergio Grinstein
10. Lisa A Robinson

Reviewed by Review Commons

Holotomographic microscopy reveals label-free quantitative dynamics of endothelial cells during endothelialization

1. William D. Leineweber
2. Gabriela Acevedo Munares
3. Christian Leycam
4. Raul Michael
5. Juliette Noyer
6. Patrick Jurney

Reviewed by Arcadia Science

Restoring mechanophenotype reverts malignant properties of ECM-enriched vocal fold cancer

1. Jasmin Kaivola
2. Karolina Punovuori
3. Megan R. Chastney
4. Yekaterina A. Miroshnikova
5. Hind Abdo
6. Fabien Bertillot
7. Fabian Krautgasser
8. Jasmin Di Franco
9. James R.W. Conway
10. Gautier Follain
11. Jaana Hagström
12. Antti Mäkitie
13. Heikki Irjala
14. Sami Ventelä
15. Hellyeh Hamidi
16. Giorgio Scita
17. Roberto Cerbino
18. Sara A. Wickström
19. Johanna Ivaska

Reviewed by preLights

A FUCCI sensor reveals complex cell cycle organization of Toxoplasma endodyogeny

1. Mrinalini Batra
2. Clem Marsilia
3. Danya Awshah
4. Lauren M. Hawkins
5. Chengqi Wang
6. Dale Chaput
7. Daria A. Naumova
8. Elena S. Suvorova

Reviewed by Review Commons

Defective Neurogenesis in Lowe Syndrome is Caused by Mitochondria Loss and Cilia-related Sonic Hedgehog Defects

1. Chien-Hui Lo
2. Siyu Chen
3. Jingyu Zhao
4. Zhiquan Liu
5. Biao Wang
6. Qing Wang
7. Tia J Kowal
8. Yang Sun

• Curated by eLife

  eLife Assessment

  This study investigated mitochondrial dysfunction and the impairment of the ciliary Sonic Hedgehog signaling in Lowe syndrome (LS), a timely topic given the limited research in this area. The data from patient iPSC-derived neurons and a mouse model were collected using solid methods, but the evidence supporting key claims is incomplete, and some technical aspects fall short of expectations. Despite these limitations, the study provides a useful foundation for exploring the relationship between mitochondrial defects and primary cilia in neural development.

Reviewed by eLife

Chemotherapy resistance due to epithelial-to-mesenchymal transition is caused by abnormal lipid metabolic balance

1. Atsushi Matsumoto
2. Akihito Inoko
3. Waki Hosoda
4. Takahiro Kojima
5. Koji Ohnishi
6. Junichi Ikenouchi

• Curated by eLife

  eLife Assessment

  This paper presents the important discovery that lipid metabolic imbalance caused by Snail, an EMT-related transcription factor, contributes to the acquisition of chemoresistance in cancer cells. However, the incomplete support for the authors' claims is due to concerns about the causal relationship and lack of sufficient quantitative analysis. With strengthened evidence, this work would be of broad interest to researchers in the fields of cancer biology, lipid metabolism, and cell biology.

Reviewed by eLife

Endocytic recycling is central to circadian collagen fibrillogenesis and disrupted in fibrosis

1. Joan Chang
2. Adam Pickard
3. Jeremy A Herrera
4. Sarah O'Keefe
5. Richa Garva
6. Matthew Hartshorn
7. Anna Hoyle
8. Lewis Dingle
9. John Knox
10. Thomas A Jowitt
11. Madeleine Coy
12. Jason Wong
13. Adam Reid
14. Yinhui Lu
15. Cédric Zeltz
16. Rajamiyer V Venkateswaran
17. Patrick T Caswell
18. Stephen High
19. Donald Gullberg
20. Karl E Kadler

• Curated by eLife

  eLife Assessment

  This study describes a novel mechanism for how collagen fibrils are formed. The authors present compelling evidence that collagen-I fibrillogenesis relies on a functional endocytic system for recycling collagen-I, with circadian-regulated VPS33b and integrin-α11 being critical for fibril assembly. This is an important study for the understanding of the pathophysiology of collagen fibrillogenesis.

Reviewed by eLife

Progesterone induces meiosis through two obligate co-receptors with PLA2 activity

1. Nancy Nader
2. Lama Assaf
3. Lubna Zarif
4. Anna Halama
5. Sharan Yadav
6. Maya Dib
7. Nabeel Attarwala
8. Qiuying Chen
9. Karsten Suhre
10. Steven Gross
11. Khaled Machaca

• Curated by eLife

  eLife Assessment

  This important study provides solid evidence for a non-genomic action of progesterone in Xenopus oocyte activation. The findings demonstrate that two non-genomic progesterone receptors, ABHD2 and mPRb, function as a novel progesterone-stimulated phospholipase A2. The findings will be of broad interest to reproductive endocrinologists and physiologists.

Reviewed by eLife