Cell Biology

Golgi compaction facilitates microtubule nucleation to drive adult vertebrate peripheral neuron regeneration

1. Alice E Mortimer
2. Adam J Reid
3. Raman M Das

Reviewed by preLights

LAPTM4B Alleviates Pulmonary Fibrosis by Enhancing NEDD4L-Mediated TGF-β Signaling Suppression

1. Kai Xu
2. Xiaoyue Pan
3. Hui Lian
4. Yaxuan Wang
5. Ruyan Wan
6. Zhongzheng Li
7. Xin Pan
8. Yajun Li
9. Juntang Yang
10. Ivan Rosas
11. Lan Wang
12. Guoying Yu

• Curated by eLife

  eLife Assessment

  In this valuable manuscript, the authors propose that the lysosomal protein LAPTM4B plays a role in suppressing the TGF-β/SMAD signaling pathway and suggest that enhancing LAPTM4B function could be a potential therapeutic strategy for alleviating bleomycin-induced lung fibrosis. The findings will be of interest to the lung disease field, and the data presented to support the authors' conclusions is solid. However, it remains unclear whether the suppressive effect of LAPTM4b on idiopathic pulmonary fibrosis is mediated by Nedd4l.

Reviewed by eLife

Cigarette smoke impairs the endocytotic process in Saccharomyces cerevisiae

1. Aditya Shukla
2. Srimonti Sarkar
3. Alok Kumar Sil

Reviewed by Arcadia Science

Global coordination of protrusive forces in migrating immune cells

1. Patricia Reis-Rodrigues
2. Nikola Canigova
3. Mario J. Avellaneda
4. Florian Gaertner
5. Kari Vaahtomeri
6. Michael Riedl
7. Jack Merrin
8. Robert Hauschild
9. Yoshinori Fukui
10. Alba Juanes Garcia
11. Michael Sixt

Reviewed by preLights

Pharmacologically inducing regenerative cardiac cells by small molecule drugs

1. Wei Zhou
2. Kezhang He
3. Chiyin Wang
4. Pengqi Wang
5. Dan Wang
6. Bowen Wang
7. Han Geng
8. Hong Lian
9. Tianhua Ma
10. Yu Nie
11. Sheng Ding

• Curated by eLife

  eLife Assessment

  This manuscript offers valuable information on the combinatory effect of small molecules, CHIR99021 and A-485 (2C), during the reprogramming of mature cardiomyocytes into regenerative cardiac cells on stimulating cardiac cell regeneration. Although the study used several hESC lines and an in vivo model of myocardial injury to demonstrate the regenerative potential of cardiac cells, the manuscript is still incomplete as several concerns remain unanswered, including the lack of validation of the conclusions from scRNA-seq. It is still unclear how a small fraction of dedifferentiating cardiac cells can offer such broad effects on regeneration both in vitro and in vivo. If validated, this study might unlock potential therapeutic strategies for cardiac regeneration.

Reviewed by eLife

Centrosome Migration and Apical Membrane Formation in Polarized Epithelial Cells: Insights from the MDCK Cyst Model

1. Po-Kai Wang
2. Keng-Hui Lin
3. Tang K Tang

• Curated by eLife

  eLife Assessment

  Wang et al's study addresses an important critical gap in our understanding of de novo epithelial polarization using MDCK cell doublets surrounded by ECM, providing convincing evidence through imaging and depletion studies on the role of conserved polarity proteins and the centrosome during this process. While the authors propose a clear hierarchical model, there is a need for further exploration of how microtubule organization contributes to this process. Specifically, live cell imaging of microtubules under mutants and their included ECM conditions, along with a more precise temporal mapping of microtubule dynamics in relation to proteins like Gp135, would strengthen the study's conclusions.

Reviewed by eLife

Ciliary length regulation by intraflagellar transport in zebrafish

1. Yi Sun
2. Zhe Chen
3. Minjun Jin
4. Haibo Xie
5. Chengtian Zhao

• Curated by eLife

  eLife Assessment

  The manuscript represents a valuable conceptual and technical contribution to our understanding of ciliogenesis and intraflagellar transport in vertebrates. Through a series of solid and technically superb live imaging experiments to directly visualize intraflagellar transport in various zebrafish ciliated tissues, the authors unveil the surprising breadth of intraflagellar transport speed among differing organs and link this to cell type-specific differences in cilia length and intraflagellar transport train size. This work will be of broad interest to researchers in numerous fields, including development, cell biology, and imaging.

Reviewed by eLife

Epigenetic deprogramming driven by disruption of CIZ1-RNA nuclear assemblies in early-stage breast cancers

1. Dawn Coverley

Reviewed by PREreview

Mechanical Centrosome Fracturing during Cell Navigation

1. Madeleine T. Schmitt
2. Mauricio J.A. Ruiz-Fernandez
3. Kasia Stefanowski
4. Janina Kroll
5. Robert Hauschild
6. Jack Merrin
7. Thomas Penz
8. Eva Kiermaier
9. Christoph Bock
10. Tobias Straub
11. Jörg Renkawitz

Reviewed by Arcadia Science

Quantitative Comparison of Monomeric StayGold Variants Using Protein Nanocages in Living Cells

1. Giulia Viola
2. Kyle A. Jacobs
3. Joël Lemière
4. Matthew L. Kutys
5. Torsten Wittmann

Reviewed by Arcadia Science